Potentiometric sensing of ions with ion-selective electrodes (ISEs) is a powerful technique for selective and sensitive measurement of ions in complex matrices. The application of ISEs is generally limited to laboratory settings, because most commercially available ISEs and reference electrodes are large, delicate, and expensive, and are not suitable for point-of-use or point-of-care measurements. This work utilizes cotton thread as a substrate for fabrication of robust and miniaturized ISEs that are suitable for point-of-care or point-of-use applications. Thread-based ISEs selective for Cl⁻, K⁺, Na⁺, and Ca²⁺ were developed. The cation-selective ISEs were fabricated by coating the thread with a surfactant-free conductive ink (made of carbon black) and then coating the tip of the conductive thread with the ion-selective membrane. The Cl⁻ ISE was fabricated by coating the thread with an Ag/AgCl ink. These sensors exhibited slopes (of electrical potential vs. log concentration of target ion), close to the theoretically-expected values, over four orders of magnitude in concentrations of ions. Because thread is mechanically strong, the thread-based electrodes can be used in multiple-use applications as well as single-use applications. Multiple thread-based sensors can be easily bundled together to fabricate a customized sensor for multiplexed ion-sensing. These electrodes require volumes of sample as low as 200 μL. The application of thread-based ISEs is demonstrated in the analysis of ions in soil, food, and dietary supplements (Cl⁻ in soil/water slurry, K⁺ and Na⁺ in coconut water, and Ca²⁺ in a calcium supplement), and in detection of physiological electrolytes (K⁺ and Na⁺ in blood serum and urine, with sufficient accuracy for clinical diagnostics).

Sustainable agricultural practices are vital to meet the needs of a rapidly growing global demand for food. Monitoring plant health is crucial for enhancing crop yields. Salicylic acid (SA) is a plant hormone that plays a key role in the defense mechanisms plants employ against various stress factors. Changes in SA levels serve as an indicator that a plant is experiencing stress. Here, we present a low-cost electrochemical sensor fabricated using porous laser-induced graphene material for the measurement of plant stress. It employs square wave voltammetry to monitor SA levels using a wireless potentiostat. The sensor shows a high sensitivity of 144.28 μA mM⁻¹ to SA, with a linear detection range of 6.6 μM to 200 μM and a limit of detection of 1.44 μM. The surface of the working electrode was modified with Nafion to enable continuous in situ stress monitoring. The sensor's practicality has been demonstrated through real-time stress monitoring in aloe vera and philodendron hederaceum plant species.

Acetylcholine (ACh) is involved in memory and learning and has implications in neurodegenerative diseases; it is therefore important to study the dynamics of ACh in the brain. This work creates a flexible solid-contact potentiometric sensor for in vitro and in vivo recording of ACh in the brain and tissue homogenate. We fabricate this sensor using a 250 μm diameter cotton yarn coated with a flexible conductive ink and an ACh sensing membrane that contains a calix[4]arene ionophore. The exposed ion-to-electron transducer was sealed with a 2.5 μm thick Parylene C coating to maintain the flexibility of the sensor. The resulting diameter of the flexible ACh sensing thread (FAST) was 400 μm. The FAST showed a linear response range from 1.0 μM to 10.0 mM in deionized water, with a near-Nernstian slope of 56.11 mV/decade and a limit of detection of 2.6 μM. In artificial cerebrospinal fluid, the limit of detection increased to 20 μM due to the background signal of ionic content of the cerebrospinal fluid. The FAST showed a signal stability of 226 μV/h over 24 h. We show that FAST can measure ACh dynamics in sheep brain tissue and sheep brain homogenate after ACh spiking. FAST is the first flexible electrochemical sensor for monitoring ACh dynamics in the brain.