Plant growth is affected by various abiotic stresses, including water, temperature, light, salt, and heavy metals. Selenium (Se) is not an essential nutrient for plants but plays important roles in alleviating the abiotic stresses suffered by plants. This article summarizes the Se uptake and metabolic processes in plants and the functions of Se in response to water, temperature, light, salt, and heavy metal stresses in plants. Se promotes the uptake of beneficial substances, maintains the stability of plasma membranes, and enhances the activity of various antioxidant enzymes, thus alleviating adverse effects in plants under abiotic stresses. Future research directions on the relationship between Se and abiotic stresses in plants are proposed. This article will further deepen our understanding of the relationship between Se and plants.

Selenium (Se) is an indispensable trace element for humans and other animals. Various studies have demonstrated the beneficial effects of Se on plants, including the promotion of growth, accumulation of secondary metabolites, and enhancement of antioxidant capacity, thereby improving plant stress resistance. Consequently, Se biofortification has emerged as an effective strategy to elevate Se content and nutritional quality in plants, attracting widespread attention. The mechanism of selenium (Se) at the plant secondary metabolic level has not yet been fully elucidated, and it remains an unanswered question as to how selenium affects plant secondary metabolic pathways and how these metabolic pathways respond to selenium biofortification. Although it has been shown that selenium can affect the antioxidant system and defense mechanisms in plants, detailed mechanisms of selenium’s action on plant secondary metabolic pathways, including its effects on specific metabolic enzymes and regulatory genes, still need to be revealed by further in-depth studies. The present study aims to elucidate the mechanisms of Se absorption, transport, and metabolism in plants under Se-rich conditions and to investigate the impact of various Se biofortification methods on the content of plant secondary metabolites. By integrating existing research progress, this paper will delve into the potential molecular regulatory mechanisms of Se on plant secondary metabolism, aiming to unravel the interplay between Se and plant secondary metabolism. This study provides a novel perspective and direction for future research on plant secondary metabolism and the biological utilization of Se.

It has been challenging to simultaneously improve photosynthesis and stress tolerance in plants. Crassulacean acid metabolism (CAM) is a CO₂-concentrating mechanism that facilitates plant adaptation to water-limited environments. We hypothesized that the ectopic expression of a CAM-specific phosphoenolpyruvate carboxylase (PEPC), an enzyme that catalyzes primary CO₂ fixation in CAM plants, would enhance both photosynthesis and abiotic stress tolerance. To test this hypothesis, we engineered a CAM-specific PEPC gene (named AaPEPC1) from Agave americana into tobacco. In comparison with wild-type and empty vector controls, transgenic tobacco plants constitutively expressing AaPEPC1 showed a higher photosynthetic rate and biomass production under normal conditions, along with significant carbon metabolism changes in malate accumulation, the carbon isotope ratio δ¹³C, and the expression of multiple orthologs of CAM-related genes. Furthermore, AaPEPC1 overexpression enhanced proline biosynthesis, and improved salt and drought tolerance in the transgenic plants. Under salt and drought stress conditions, the dry weight of transgenic tobacco plants overexpressing AaPEPC1 was increased by up to 81.8% and 37.2%, respectively, in comparison with wild-type plants. Our findings open a new door to the simultaneous improvement of photosynthesis and stress tolerance in plants.

Cadmium (Cd) is a common agricultural soil pollutant, which does serious harm to the environment and the human body. In this study, Brassica juncea was treated with different concentrations of CdCl₂ and Na₂SeO₃. Then, physiological indexes and transcriptome were measured to reveal the mechanisms by which Se reduces the inhibition and toxicity of Cd in B. juncea. The results showed that Se alleviated the inhibitive Cd effects on seedling biomass, root length, and chlorophyll, and promoted the adsorption of Cd by pectin and lignin in the root cell wall (CW). Se also alleviated the oxidative stress induced by Cd, and reduced the content of MDA in cells. As a result, SeCys and SeMet alleviated the transport of Cd to the shoots. Transcriptome data showed that the bivalent cation transporter MPP and ABCC subfamily participated in the separation of Cd in vacuoles, CAL1 was related to the chelation of Cd in the cytoplasm of cells, and ZIP transporter 4 reduced the transport of Cd to the shoots. These results indicated that Se alleviated the damage of Cd in plants and decreased its transport to the shoots by improving the antioxidant system, enhancing the ability of the CW to adsorb Cd, reducing the activity of Cd transporters, and chelating Cd.

Resistant starch (RS) refers to starch varieties that resist digestion by human digestive enzymes. Owing to its distinctive physicochemical attributes and functional capabilities, RS has gained a wide range of applications as a dietary fiber and prebiotic. In terms of structure and functions, RS can be categorized into five distinct types: RS1 through RS5. These types offer dietary benefits, contributing to improved colonic health, the modulation of microbial communities, the reduction in gallstone formation, the enhancement of mineral absorption, and alterations in fat oxidation potential. From a technical standpoint, RS can be manufactured through an array of physical, enzymatic, and chemical modifications. This paper presents a comprehensive review of the existing literature, summarizing the classification, structural features, raw material origins, preparation methodologies, and functionalities of RS. Furthermore, new production technologies and applications of RS, such as 3D printing, provide valuable insights.