Epicoccum nigrum Link is a cosmopolitan species, and it has been described as both an in vitro and in vivo antagonist of many fungal pathogens of plants. However, there are no clear reports about the interactions between E. nigrum and various plant species, and about the effects of culture filtrates produced by this fungus on plants. Therefore, we assessed the interactions between E. nigrum and different plant species, such as sugar beet (Beta vulgaris L. ssp. vulgaris), spring wheat (Triticum aestivum L.), red clover (Trifolium pratense L.), and winter oilseed rape (Brassica napus L.). Additionally, we evaluated the effect of E. nigrum culture filtrates on garden cress (Lepidium sativum L.). Our study showed that the E. nigrum strains varied in terms of the color of excreted culture filtrates and showed different interactions with garden cress. Overall, fungal strains only affected adversely the sprout length in a significant way and, partially, the growth of the tested plant. In addition, we confirmed the suitability of the garden cress as a test plant in in vitro toxicological tests. Most strains of E. nigrum (61.1%) secreted enzymes expected to participate mainly in the later stages of the infection (amylases and proteases) and not those expected to operate in the early phases of host penetration (cellulases and pectinases) that were secreted by 33.3% of fungal strains. The group of pectinolytic enzymes represented the catalysts with the highest activity. Host specialization tests showed that E. nigrum was mainly re-isolated from the plant surface and the number of infected seedlings as well as the disease index depended on a studied plant species, with sugar beet and red clover being most sensitive to infection. In turn, the lowest value of the disease index caused by E. nigrum strains was recorded for spring wheat and winter oilseed rape. Overall, statistically significant differences in the growth of plant seedlings during the host specialization test were noted only for sugar beet and red clover seedlings. The seedlings of plants in the control group (without fungal inoculum) exhibited an increased length compared to those treated with E. nigrum inoculum. Our studies also showed that E. nigrum is probably a facultative saprotroph of plants and it may winter on red clover, which is presumably its main reservoirs, among the species considered.

The research concerned the elaborate of non-waste biogas production technology based on the development of digestate from anaerobic digestion. In the anaerobic digestion process, the substrates of plant origin in the form of silage were used. The digestate obtained after biogas production was processed using the ORTWED method into a valuable granulated organic-mineral fertilizer, which contains a solid fraction of digestate, calcium and biogenic elements. This method can be successfully applied in agriculture in the context of its sustainable development due to the growing problem of utilization of digestate forming in agricultural biogas plants.

Harrowing is one of the most popular mechanical methods to control weeds. Nevertheless, the relationship between the effect of different harrowing intensities using spike-tooth harrow in barley-pea intercrop on the yield and mycoflora of grains has not yet been studied. Therefore, the aim of this study was to assess the effect of harrow intensity using spike-tooth harrow in barley-pea mixture on the mycological quality of harvested grains, grain yield, as well as influence of barley and pea grain moisture on the abundance of fungi. The field experiment was carried out during 2010–2012, and it was conducted using a randomized complete block design with four replicates. Weed control was mechanical and chemical. In this study, we have shown that harrowing in barley-pea intercrops does not reduce the yield of either mixture components, both with respect to grain quantity or mycological quality after harvest, compared to controls—without harrowing and the herbicide MCPA (2-methyl-4-chlorophenoxyacetic acid). However, increasing the intensity of harrowing did not result in a consistently larger crop yield or reduction in fungal abundance in the grains. The grains’ internal structures and surface of both tested components of the mixture were colonized to a large extent by cosmopolitan fungi, of which Alternaria alternata (Fr.) Keissl. was the most abundant.

The aim of the conducted observations was to examine the effect of fertiliser produced based on plant digestate and resulting from the process of anaerobic fermentation of a substrate with sorghum (20%), beet (60%), rye (10%) and grass (10%) on the growth and yield of sorghum and corn. A strict, single-factor pot experiment was carried out in 2017–2018 in the Swojec Agricultural Experimental Unit at the University of Life Sciences in Wrocław. Fertilisation with digestate was the tested factor, with a focus on its influence on selected energy plants. Sucrosorgo sorghum and Atletico maize were analysed. During the research, the pots were placed in a vegetation hall. The research proved that the use of digestate-based fertiliser improves the productivity of both sorghum and maize, but only with an additional dose of basic macronutrients. The optimal variant is a fertiliser dose of 20 g enriched with 1.0 NPK. Increasing the dose to 30 g and 1.5 NPK generally did not result in a significant increase in plant height or in fresh and dry weights.

One of the most common annual overwintering weeds in various crops worldwide is silky bent grass (Aspera spica-venti (L.) Beauv). The aim of this study was to investigate the selected morphological characteristics and chemical composition of A. spica-venti from different cultivation fields in Poland, depending on the macronutrient content of the soil. The average pH values of the soil samples, and the average concentration of nitrogen (N) and phosphorus (P) in the samples were statistically different between study sites. In turn, the concentration of potassium (K) was at the same statistical level. The average values of N, P and K concentrations in the straw of A. spica-venti (% dry matter) in particular study sites were not statistically different. The mean values of the examined morphological features of A. spica-venti did not differ statistically between the individual test sites. Overall, there was no significant impact of the habitat on the chemical composition and morphology of the A. spica-venti occurring naturally in arable fields. However, an increase in soil abundance in some macronutrients (mainly K) may positively affect the morphology of this weed as opposed to an increase in nitrogen concentration in the soil, and an increase soil pH. The lack of significant influence of soil conditions and location of APESV sites on the morphology and chemical composition of the weed indicates that it has high plasticity and is able to thrive under varying habitat conditions.