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Dr. Gian Paolo Accotto
IPSP-CNR

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0 Virus Evolution
0 Virus discovery
0 virus host interaction
0 Plant viruses
0 plant virus vector relationships

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Journal article
Published: 02 July 2021 in Cells
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Seed transmission is an important factor in the epidemiology of plant pathogens. Geminiviruses are serious pests spread in tropical and subtropical regions. They are transmitted by hemipteran insects, but a few cases of transmission through seeds were recently reported. Here, we investigated the tomato seed transmissibility of the begomovirus tomato yellow leaf curl Sardinia virus (TYLCSV), one of the agents inducing the tomato yellow leaf curl disease, heavily affecting tomato crops in the Mediterranean area. None of the 180 seedlings originating from TYLCSV-infected plants showed any phenotypic alteration typical of virus infection. Moreover, whole viral genomic molecules could not be detected in their cotyledons and true leaves, neither by membrane hybridization nor by rolling-circle amplification followed by PCR, indicating that TYLCSV is not a seed-transmissible pathogen for tomato. Examining the localization of TYLCSV DNA in progenitor plants, we detected the virus genome by PCR in all vegetative and reproductive tissues, but viral genomic and replicative forms were found only in leaves, flowers and fruit flesh, not in seeds and embryos. Closer investigations allowed us to discover for the first time that these embryos were superficially contaminated by TYLCSV DNA but whole genomic molecules were not detectable. Therefore, the inability of TYLCSV genomic molecules to colonize tomato embryos during infection justifies the lack of seed transmissibility observed in this host.

ACS Style

Saeid Tabein; Laura Miozzi; Slavica Matić; Gian Accotto; Emanuela Noris. No Evidence for Seed Transmission of Tomato Yellow Leaf Curl Sardinia Virus in Tomato. Cells 2021, 10, 1673 .

AMA Style

Saeid Tabein, Laura Miozzi, Slavica Matić, Gian Accotto, Emanuela Noris. No Evidence for Seed Transmission of Tomato Yellow Leaf Curl Sardinia Virus in Tomato. Cells. 2021; 10 (7):1673.

Chicago/Turabian Style

Saeid Tabein; Laura Miozzi; Slavica Matić; Gian Accotto; Emanuela Noris. 2021. "No Evidence for Seed Transmission of Tomato Yellow Leaf Curl Sardinia Virus in Tomato." Cells 10, no. 7: 1673.

Journal article
Published: 22 June 2020 in Viruses
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Tomato plants can establish symbiotic interactions with arbuscular mycorrhizal fungi (AMF) able to promote plant nutrition and prime systemic plant defenses against pathogens attack; the mechanism involved is known as mycorrhiza-induced resistance (MIR). However, studies on the effect of AMF on viral infection, still limited and not conclusive, indicate that AMF colonization may have a detrimental effect on plant defenses against viruses, so that the term “mycorrhiza-induced susceptibility” (MIS) has been proposed for these cases. To expand the case studies to a not yet tested viral family, that is, Bromoviridae, we investigated the effect of the colonization by the AMF Funneliformis mosseae on cucumber mosaic virus (CMV) infection in tomato by phenotypic, physiological, biochemical, and transcriptional analyses. Our results showed that the establishment of a functional AM symbiosis is able to limit symptoms development. Physiological and transcriptomic data highlighted that AMF mitigates the drastic downregulation of photosynthesis-related genes and the reduction of photosynthetic CO2 assimilation rate caused by CMV infection. In parallel, an increase of salicylic acid level and a modulation of reactive oxygen species (ROS)-related genes, toward a limitation of ROS accumulation, was specifically observed in CMV-infected mycorrhizal plants. Overall, our data indicate that the AM symbiosis influences the development of CMV infection in tomato plants and exerts a priming effect able to enhance tolerance to viral infection.

ACS Style

Laura Miozzi; Anna Maria Vaira; Federico Brilli; Valerio Casarin; Mara Berti; Alessandra Ferrandino; Luca Nerva; Gian Paolo Accotto; Luisa Lanfranco. Arbuscular Mycorrhizal Symbiosis Primes Tolerance to Cucumber Mosaic Virus in Tomato. Viruses 2020, 12, 1 .

AMA Style

Laura Miozzi, Anna Maria Vaira, Federico Brilli, Valerio Casarin, Mara Berti, Alessandra Ferrandino, Luca Nerva, Gian Paolo Accotto, Luisa Lanfranco. Arbuscular Mycorrhizal Symbiosis Primes Tolerance to Cucumber Mosaic Virus in Tomato. Viruses. 2020; 12 (6):1.

Chicago/Turabian Style

Laura Miozzi; Anna Maria Vaira; Federico Brilli; Valerio Casarin; Mara Berti; Alessandra Ferrandino; Luca Nerva; Gian Paolo Accotto; Luisa Lanfranco. 2020. "Arbuscular Mycorrhizal Symbiosis Primes Tolerance to Cucumber Mosaic Virus in Tomato." Viruses 12, no. 6: 1.

Journal article
Published: 04 March 2019 in BMC Genomics
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Small RNAs (sRNAs) are short non-coding RNA molecules (20–30 nt) that regulate gene expression at transcriptional or post-transcriptional levels in many eukaryotic organisms, through a mechanism known as RNA interference (RNAi). Recent studies have highlighted that they are also involved in cross-kingdom communication: sRNAs can move across the contact surfaces from “donor” to “receiver” organisms and, once in the host cells of the receiver, they can target specific mRNAs, leading to a modulation of host metabolic pathways and defense responses. Very little is known about RNAi mechanism and sRNAs occurrence in Arbuscular Mycorrhizal Fungi (AMF), an important component of the plant root microbiota that provide several benefits to host plants, such as improved mineral uptake and tolerance to biotic and abiotic stress. Taking advantage of the available genomic resources for the AMF Rhizophagus irregularis we described its putative RNAi machinery, which is characterized by a single Dicer-like (DCL) gene and an unusual expansion of Argonaute-like (AGO-like) and RNA-dependent RNA polymerase (RdRp) gene families. In silico investigations of previously published transcriptomic data and experimental assays carried out in this work provided evidence of gene expression for most of the identified sequences. Focusing on the symbiosis between R. irregularis and the model plant Medicago truncatula, we characterized the fungal sRNA population, highlighting the occurrence of an active sRNA-generating pathway and the presence of microRNA-like sequences. In silico analyses, supported by host plant degradome data, revealed that several fungal sRNAs have the potential to target M. truncatula transcripts, including some specific mRNA already shown to be modulated in roots upon AMF colonization. The identification of RNAi-related genes, together with the characterization of the sRNAs population, suggest that R. irregularis is equipped with a functional sRNA-generating pathway. Moreover, the in silico analysis predicted 237 plant transcripts as putative targets of specific fungal sRNAs suggesting that cross-kingdom post-transcriptional gene silencing may occur during AMF colonization.

ACS Style

Alessandro Silvestri; Valentina Fiorilli; Laura Miozzi; Gian Paolo Accotto; Massimo Turina; Luisa Lanfranco. In silico analysis of fungal small RNA accumulation reveals putative plant mRNA targets in the symbiosis between an arbuscular mycorrhizal fungus and its host plant. BMC Genomics 2019, 20, 1 -18.

AMA Style

Alessandro Silvestri, Valentina Fiorilli, Laura Miozzi, Gian Paolo Accotto, Massimo Turina, Luisa Lanfranco. In silico analysis of fungal small RNA accumulation reveals putative plant mRNA targets in the symbiosis between an arbuscular mycorrhizal fungus and its host plant. BMC Genomics. 2019; 20 (1):1-18.

Chicago/Turabian Style

Alessandro Silvestri; Valentina Fiorilli; Laura Miozzi; Gian Paolo Accotto; Massimo Turina; Luisa Lanfranco. 2019. "In silico analysis of fungal small RNA accumulation reveals putative plant mRNA targets in the symbiosis between an arbuscular mycorrhizal fungus and its host plant." BMC Genomics 20, no. 1: 1-18.

Journal article
Published: 08 January 2019 in New Disease Reports
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ACS Style

Nabil Radouane; S. Ezrari; G.P. Accotto; M. Benjelloun; R. Lahlali; A. Tahiri; A.M. Vaira. First report of Chickpea chlorotic dwarf virus in watermelon ( Citrullus lanatus ) in Morocco. New Disease Reports 2019, 39, 2 -2.

AMA Style

Nabil Radouane, S. Ezrari, G.P. Accotto, M. Benjelloun, R. Lahlali, A. Tahiri, A.M. Vaira. First report of Chickpea chlorotic dwarf virus in watermelon ( Citrullus lanatus ) in Morocco. New Disease Reports. 2019; 39 (1):2-2.

Chicago/Turabian Style

Nabil Radouane; S. Ezrari; G.P. Accotto; M. Benjelloun; R. Lahlali; A. Tahiri; A.M. Vaira. 2019. "First report of Chickpea chlorotic dwarf virus in watermelon ( Citrullus lanatus ) in Morocco." New Disease Reports 39, no. 1: 2-2.

Journal article
Published: 13 December 2018 in Nature Communications
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The propensity of viruses to acquire genetic material from relatives and possibly from infected hosts makes them excellent candidates as vectors for horizontal gene transfer. However, virus-mediated acquisition of host genetic material, as deduced from historical events, appears to be rare. Here, we report spontaneous and surprisingly efficient generation of hybrid virus/host DNA molecules in the form of minicircles during infection of Beta vulgaris by Beet curly top Iran virus (BCTIV), a single-stranded DNA virus. The hybrid minicircles replicate, become encapsidated into viral particles, and spread systemically throughout infected plants in parallel with the viral infection. Importantly, when co-infected with BCTIV, B. vulgaris DNA captured in minicircles replicates and is transcribed in other plant species that are sensitive to BCTIV infection. Thus, we have likely documented in real time the initial steps of a possible path of virus-mediated horizontal transfer of chromosomal DNA between plant species.

ACS Style

Marco Catoni; Emanuela Noris; Anna Maria Vaira; Thomas Jonesman; Slavica Matić; Reihaneh Soleimani; Seyed Ali Akbar Behjatnia; Nestor Vinals; Jerzy Paszkowski; Gian Paolo Accotto. Virus-mediated export of chromosomal DNA in plants. Nature Communications 2018, 9, 5308 .

AMA Style

Marco Catoni, Emanuela Noris, Anna Maria Vaira, Thomas Jonesman, Slavica Matić, Reihaneh Soleimani, Seyed Ali Akbar Behjatnia, Nestor Vinals, Jerzy Paszkowski, Gian Paolo Accotto. Virus-mediated export of chromosomal DNA in plants. Nature Communications. 2018; 9 (1):5308.

Chicago/Turabian Style

Marco Catoni; Emanuela Noris; Anna Maria Vaira; Thomas Jonesman; Slavica Matić; Reihaneh Soleimani; Seyed Ali Akbar Behjatnia; Nestor Vinals; Jerzy Paszkowski; Gian Paolo Accotto. 2018. "Virus-mediated export of chromosomal DNA in plants." Nature Communications 9, no. 1: 5308.

Preprint
Published: 26 November 2018
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Arundo donax L. is an invasive species recently employed for biomass production that emits large amounts of isoprene, a volatile compound having important defensive role. Here, the potential of A. donax to grow in degraded soils, characterized by poor fertility, eutrophication and/or salinization, has been evaluated at morphological, biochemical and transcriptional level. Our results highlight sensitivity of A. donax to P deficiency. Moreover, we show that A. donax response to salt stress (high sodium, Na+), which impaired plant performance causing detrimental effects on leaf cells ultrastructure, is characterized by enhanced biosynthesis of antioxidant carotenoids and sucrose. Differently from Na+, high phosphorous (P) supply did not hamper photosynthesis although it affected carbon metabolism through reduction of starch content and by lowering isoprene emission. In particular, we revealed on salt-stress leaves that high P enhanced the expression of genes involved in abiotic stress tolerance, but further increased diffusive limitations to photosynthesis and slowed-down sugar turnover without modifying isoprene emission. Therefore, despite limiting productivity, high P improved A. donax tolerance to salinity by favouring the accumulation of carbohydrates that protect cells and increase osmotic potential, and by stimulating the synthesis of antioxidants that improves photo-protection and avoids excessive accumulation of reactive oxygen species.HighlightsArundo donax is sensitive to elevated salinity. High phosphorous supply to salt-stressed A. donax enhances transcriptomic changesthat induce the onset of physiological mechanisms of stress tolerance but limits productivity.

ACS Style

Claudia Cocozza; Federico Brilli; Laura Miozzi; Sara Pignattelli; Silvia Rotunno; Cecilia Brunetti; Cristiana Giordano; Susanna Pollastri; Mauro Centritto; Gian Paolo Accotto; Roberto Tognetti; Francesco Loreto. Impact of high phosphorous and sodium on productivity and stress tolerance of Arundo donax plants. 2018, 477810 .

AMA Style

Claudia Cocozza, Federico Brilli, Laura Miozzi, Sara Pignattelli, Silvia Rotunno, Cecilia Brunetti, Cristiana Giordano, Susanna Pollastri, Mauro Centritto, Gian Paolo Accotto, Roberto Tognetti, Francesco Loreto. Impact of high phosphorous and sodium on productivity and stress tolerance of Arundo donax plants. . 2018; ():477810.

Chicago/Turabian Style

Claudia Cocozza; Federico Brilli; Laura Miozzi; Sara Pignattelli; Silvia Rotunno; Cecilia Brunetti; Cristiana Giordano; Susanna Pollastri; Mauro Centritto; Gian Paolo Accotto; Roberto Tognetti; Francesco Loreto. 2018. "Impact of high phosphorous and sodium on productivity and stress tolerance of Arundo donax plants." , no. : 477810.

Review article
Published: 15 March 2018 in Frontiers in Plant Science
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Cicer arietinum L. (chickpea) is the world's fourth most widely grown pulse. Chickpea seeds are a primary source of dietary protein for humans, and chickpea cultivation contributes to biological nitrogen fixation in the soil, given its symbiotic relationship with rhizobia. Therefore, chickpea cultivation plays a pivotal role in innovative sustainable models of agro-ecosystems inserted in crop rotation in arid and semi-arid environments for soil improvement and the reduction of chemical inputs. Indeed, the arid and semi-arid tropical zones of Africa and Asia have been primary areas of cultivation and diversification. Yet, nowadays, chickpea is gaining prominence in Canada, Australia, and South America where it constitutes a main ingredient in vegetarian and vegan diets. Viruses and plant parasitic nematodes (PPNs) have been considered to be of minor and local impact in primary areas of cultivation. However, the introduction of chickpea in new environments exposes the crop to these biotic stresses, compromising its yields. The adoption of high-throughput genomic technologies, including genome and transcriptome sequencing projects by the chickpea research community, has provided major insights into genome evolution as well as genomic architecture and domestication. This review summarizes the major viruses and PPNs that affect chickpea cultivation worldwide. We also present an overview of the current state of chickpea genomics. Accordingly, we explore the opportunities that genomics, post-genomics and novel editing biotechnologies are offering in order to understand chickpea diseases and stress tolerance and to design innovative control strategies.

ACS Style

Paola Leonetti; Gian Paolo Accotto; Moemen S. Hanafy; Vitantonio Pantaleo. Viruses and Phytoparasitic Nematodes of Cicer arietinum L.: Biotechnological Approaches in Interaction Studies and for Sustainable Control. Frontiers in Plant Science 2018, 9, 1 .

AMA Style

Paola Leonetti, Gian Paolo Accotto, Moemen S. Hanafy, Vitantonio Pantaleo. Viruses and Phytoparasitic Nematodes of Cicer arietinum L.: Biotechnological Approaches in Interaction Studies and for Sustainable Control. Frontiers in Plant Science. 2018; 9 ():1.

Chicago/Turabian Style

Paola Leonetti; Gian Paolo Accotto; Moemen S. Hanafy; Vitantonio Pantaleo. 2018. "Viruses and Phytoparasitic Nematodes of Cicer arietinum L.: Biotechnological Approaches in Interaction Studies and for Sustainable Control." Frontiers in Plant Science 9, no. : 1.

Note
Published: 01 February 2018 in Plant Disease
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ACS Style

G. Parrella; E. Troiano; G. Formisano; Gian Paolo Accotto; Massimo Giorgini. First Report of Tomato leaf curl New Delhi virus Associated with Severe Mosaic of Pumpkin in Italy. Plant Disease 2018, 102, 459 .

AMA Style

G. Parrella, E. Troiano, G. Formisano, Gian Paolo Accotto, Massimo Giorgini. First Report of Tomato leaf curl New Delhi virus Associated with Severe Mosaic of Pumpkin in Italy. Plant Disease. 2018; 102 (2):459.

Chicago/Turabian Style

G. Parrella; E. Troiano; G. Formisano; Gian Paolo Accotto; Massimo Giorgini. 2018. "First Report of Tomato leaf curl New Delhi virus Associated with Severe Mosaic of Pumpkin in Italy." Plant Disease 102, no. 2: 459.

Journal article
Published: 25 October 2017 in Viruses
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Chickpea chlorotic dwarf virus (CpCDV), a polyphagous mastrevirus, family Geminiviridae, has been recently linked to the onset of the “hard fruit syndrome” of watermelon, first described in Tunisia, that makes fruits unmarketable due to the presence of white hard portions in the flesh, chlorotic mottling on the rind, and an unpleasant taste. To investigate the etiological agent of this disease, total RNA extracted from symptomatic watermelon fruits was subjected to small RNA sequencing through next generation sequencing (NGS) techniques. Data obtained showed the presence of CpCDV and two other viral species. However, following validation through polymerase chain reaction (PCR), CpCDV was the only viral species consistently detected in all samples. Watermelon seedlings were then challenged by an agroinfectious CpCDV clone; several plants proved to be CpCDV-infected, and were able to produce fruits. CpCDV infected and replicated in watermelon fruits and leaves, leading to abnormality in fruits and in seed production, similar to those described in field. These results indicate that CpCDV is the etiological agent of the “hard fruit syndrome” of watermelon.

ACS Style

Takoua Zaagueri; Laura Miozzi; Monia Mnari-Hattab; Emanuela Noris; Gian Paolo Accotto; Anna Maria Vaira. Deep Sequencing Data and Infectivity Assays Indicate that Chickpea Chlorotic Dwarf Virus is the Etiological Agent of the “Hard Fruit Syndrome” of Watermelon. Viruses 2017, 9, 311 .

AMA Style

Takoua Zaagueri, Laura Miozzi, Monia Mnari-Hattab, Emanuela Noris, Gian Paolo Accotto, Anna Maria Vaira. Deep Sequencing Data and Infectivity Assays Indicate that Chickpea Chlorotic Dwarf Virus is the Etiological Agent of the “Hard Fruit Syndrome” of Watermelon. Viruses. 2017; 9 (11):311.

Chicago/Turabian Style

Takoua Zaagueri; Laura Miozzi; Monia Mnari-Hattab; Emanuela Noris; Gian Paolo Accotto; Anna Maria Vaira. 2017. "Deep Sequencing Data and Infectivity Assays Indicate that Chickpea Chlorotic Dwarf Virus is the Etiological Agent of the “Hard Fruit Syndrome” of Watermelon." Viruses 9, no. 11: 311.

Journal article
Published: 16 October 2017 in Viruses
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Beet curly top virus (BCTV) and beet curly top Iran virus (BCTIV) are known as the causal agents of curly top disease in beet and several other dicotyledonous plants in Iran. These viruses are transmitted by Circulifer species, and until now, there has been no confirmed report of their seed transmission. A percentage (38.2–78.0%) of the seedlings developed from the seeds of a petunia local cultivar under insect-free conditions showed stunting, interveinal chlorosis, leaf curling, and vein swelling symptoms, and were infected by BCTV when tested by PCR. Presence of BCTV in seed extracts of petunia local cultivar was confirmed by PCR and IC-PCR, followed by sequencing. Agroinoculation of curly top free petunia plants with a BCTV infectious clone resulted in BCTV infection of plants and their developed seeds. These results show the seed infection and transmission of BCTV in a local cultivar of petunia. Similar experiments performed with BCTIV showed that this virus is also seed transmissible in the same cultivar of petunia, although with a lower rate (8.8–18.5%). Seed transmission of curly top viruses may have significant implications in the epidemiology of these viruses.

ACS Style

Ameneh Anabestani; Seyed Ali Akbar Behjatnia; Keramat Izadpanah; Saeid Tabein; Gian Paolo Accotto. Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran. Viruses 2017, 9, 299 .

AMA Style

Ameneh Anabestani, Seyed Ali Akbar Behjatnia, Keramat Izadpanah, Saeid Tabein, Gian Paolo Accotto. Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran. Viruses. 2017; 9 (10):299.

Chicago/Turabian Style

Ameneh Anabestani; Seyed Ali Akbar Behjatnia; Keramat Izadpanah; Saeid Tabein; Gian Paolo Accotto. 2017. "Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran." Viruses 9, no. 10: 299.

Original paper
Published: 14 June 2017 in Australasian Plant Pathology
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Tomato leaf curl disease is one of the main constraints in tomato production worldwide. It is induced by viruses including Tomato leaf curl virus (ToLCV). Plant viruses can both induce and be themselves targeted by gene silencing machinery. Certain animal viruses were found to be targeted by host miRNAs but, there is no similar experimental report for plant viruses. In this study, we investigated if tomato-encoded miRNAs target ToLCV using an in silico analysis. Results showed that ToLCV can be targeted by three tomato miRNAs, named miR156, miR159, and miR403. Following virus infection of susceptible tomato plants cv Moneymaker, miR159 and miR403 were upregulated, while miR156 was unchanged. In the plants of the resistant tomato variety PS550, that accumulated 50-fold less viral DNA and did not show disease symptoms, miR156 was upregulated, while the two others remained unchanged. In addition, the expression level of three genes known as targets of these miRNAs, i.e. SPL6b, MYB33 and AGO2a, was measured in both susceptible and resistant tomato varieties by qRT-PCR. The possible role of the predicted miRNAs and regulation of their target genes in symptom development by ToLCV is discussed.

ACS Style

N. Tousi; Omid Eini; R. Ahmadvand; A. Carra; Laura Miozzi; E. Noris; G. P. Accotto. In silico prediction of miRNAs targeting ToLCV and their regulation in susceptible and resistant tomato plants. Australasian Plant Pathology 2017, 46, 379 -386.

AMA Style

N. Tousi, Omid Eini, R. Ahmadvand, A. Carra, Laura Miozzi, E. Noris, G. P. Accotto. In silico prediction of miRNAs targeting ToLCV and their regulation in susceptible and resistant tomato plants. Australasian Plant Pathology. 2017; 46 (4):379-386.

Chicago/Turabian Style

N. Tousi; Omid Eini; R. Ahmadvand; A. Carra; Laura Miozzi; E. Noris; G. P. Accotto. 2017. "In silico prediction of miRNAs targeting ToLCV and their regulation in susceptible and resistant tomato plants." Australasian Plant Pathology 46, no. 4: 379-386.

Note
Published: 01 February 2017 in Plant Disease
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ACS Style

T. Zaagueri; M. Mnari-Hattab; S. Zammouri; M. R. Hajlaoui; Gian Paolo Accotto; Annamaria Vaira. First Report of Chickpea chlorotic dwarf virus in Watermelon (Citrullus lanatus) in Tunisia. Plant Disease 2017, 101, 392 .

AMA Style

T. Zaagueri, M. Mnari-Hattab, S. Zammouri, M. R. Hajlaoui, Gian Paolo Accotto, Annamaria Vaira. First Report of Chickpea chlorotic dwarf virus in Watermelon (Citrullus lanatus) in Tunisia. Plant Disease. 2017; 101 (2):392.

Chicago/Turabian Style

T. Zaagueri; M. Mnari-Hattab; S. Zammouri; M. R. Hajlaoui; Gian Paolo Accotto; Annamaria Vaira. 2017. "First Report of Chickpea chlorotic dwarf virus in Watermelon (Citrullus lanatus) in Tunisia." Plant Disease 101, no. 2: 392.

Original articles
Published: 01 February 2017 in Archives of Phytopathology and Plant Protection
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Tomato yellow leaf curl disease is a major constraint for tomato production worldwide and availability of new resistant materials is of great importance for breeding programmes. A phenotypic survey was undertaken to evaluate the level of resistance to the main tomato yellow leaf curl disease-inducing viruses Tomato yellow leaf curl virus and Tomato yellow leaf curl Sardinia virus, in several commercial tomato cultivars, never characterised before. Seven weeks post inoculation, two cultivars resulted in high resistant phenotypes to both begomoviruses, and four were tolerant to at least one of them. In the two highly resistant hybrids (SJ12, RFT112), symptoms were completely absent and viral DNA was from 102 to 105 fold lower than in susceptible plants. Molecular marker analysis revealed that these genotypes harbour the resistant genes Ty-1/Ty-3 and Ty-2. Given their high resistance, they can be considered good candidates for cultivation and breeding in areas where incidence of TYLCD is very elevated.

ACS Style

Saeid Tabein; Seyed Ali Akbar Behjatnia; Luca Laviano; Nicola Pecchioni; Gian Paolo Accotto; Emanuela Noris; Laura Miozzi. Pyramiding Ty-1/Ty-3 and Ty-2 in tomato hybrids dramatically inhibits symptom expression and accumulation of tomato yellow leaf curl disease inducing viruses. Archives of Phytopathology and Plant Protection 2017, 50, 213 -227.

AMA Style

Saeid Tabein, Seyed Ali Akbar Behjatnia, Luca Laviano, Nicola Pecchioni, Gian Paolo Accotto, Emanuela Noris, Laura Miozzi. Pyramiding Ty-1/Ty-3 and Ty-2 in tomato hybrids dramatically inhibits symptom expression and accumulation of tomato yellow leaf curl disease inducing viruses. Archives of Phytopathology and Plant Protection. 2017; 50 (5-6):213-227.

Chicago/Turabian Style

Saeid Tabein; Seyed Ali Akbar Behjatnia; Luca Laviano; Nicola Pecchioni; Gian Paolo Accotto; Emanuela Noris; Laura Miozzi. 2017. "Pyramiding Ty-1/Ty-3 and Ty-2 in tomato hybrids dramatically inhibits symptom expression and accumulation of tomato yellow leaf curl disease inducing viruses." Archives of Phytopathology and Plant Protection 50, no. 5-6: 213-227.

Journal article
Published: 01 February 2016 in Sensors and Actuators B: Chemical
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The measurement of the intensity of light reflected by interfaces with extremely low reflectivity in water enables the label-free, multiplex detection of bio-molecular interactions. The binding of targets in solution to surface-immobilized probes (e.g. antibodies) can be quantified using a simple instrumental set-up, comprising a LED source and a camera. The condition of low reflectivity can be achieved by using a perfluorinated plastic substrate with refractive index very close to that of water. Here we show that, despite its simplicity, the method enables label-free detection in complex samples with high absorbance and turbidity. Diagnostic markers of Tomato spotted wilt virus are revealed in crude plant extracts of Datura stramonium leaves at early stage of infection. The measuring procedure requires only the addition of the sample into a cuvette and the acquisition of a series of images for a few minutes. The light reflectivity of the spots of specific antibodies increases more rapidly if the sample is prepared with infected plants.

ACS Style

Matteo Salina; Fabio Giavazzi; Erica Ceccarello; Francesco Damin; Marcella Chiari; Marina Ciuffo; Gian Paolo Accotto; Marco Buscaglia. Multi-spot, label-free detection of viral infection in complex media by a non-reflecting surface. Sensors and Actuators B: Chemical 2016, 223, 957 -962.

AMA Style

Matteo Salina, Fabio Giavazzi, Erica Ceccarello, Francesco Damin, Marcella Chiari, Marina Ciuffo, Gian Paolo Accotto, Marco Buscaglia. Multi-spot, label-free detection of viral infection in complex media by a non-reflecting surface. Sensors and Actuators B: Chemical. 2016; 223 ():957-962.

Chicago/Turabian Style

Matteo Salina; Fabio Giavazzi; Erica Ceccarello; Francesco Damin; Marcella Chiari; Marina Ciuffo; Gian Paolo Accotto; Marco Buscaglia. 2016. "Multi-spot, label-free detection of viral infection in complex media by a non-reflecting surface." Sensors and Actuators B: Chemical 223, no. : 957-962.

Research article
Published: 28 February 2014 in PLOS ONE
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Tomato yellow leaf curl Sardinia virus (TYLCSV), a DNA virus belonging to the genus Begomovirus, causes severe losses in tomato crops. It infects only a limited number of cells in the vascular tissues, making difficult to detect changes in host gene expression linked to its presence. Here we present the first microarray study of transcriptional changes induced by the phloem-limited geminivirus TYLCSV infecting tomato, its natural host. The analysis was performed on the midrib of mature leaves, a material naturally enriched in vascular tissues. A total of 2206 genes were up-regulated and 1398 were down-regulated in infected plants, with an overrepresentation of genes involved in hormone metabolism and responses, nucleic acid metabolism, regulation of transcription, ubiquitin-proteasome pathway and autophagy among those up-regulated, and in primary and secondary metabolism, phosphorylation, transcription and methylation-dependent chromatin silencing among those down-regulated. Our analysis showed a series of responses, such as the induction of GA- and ABA-responsive genes, the activation of the autophagic process and the fine tuning of the plant immune system, observed only in TYLCSV-tomato compatible interaction so far. On the other hand, comparisons with transcriptional changes observed in other geminivirus-plant interactions highlighted common host responses consisting in the deregulation of biotic stress responsive genes, key enzymes in the ethylene biosynthesis and methylation cycle, components of the ubiquitin proteasome system and DNA polymerases II. The involvement of conserved miRNAs and of solanaceous- and tomato-specific miRNAs in geminivirus infection, investigated by integrating differential gene expression data with miRNA targeting data, is discussed.

ACS Style

Laura Miozzi; Chiara Napoli; Luca Sardo; Gian Paolo Accotto. Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection. PLOS ONE 2014, 9, e89951 .

AMA Style

Laura Miozzi, Chiara Napoli, Luca Sardo, Gian Paolo Accotto. Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection. PLOS ONE. 2014; 9 (2):e89951.

Chicago/Turabian Style

Laura Miozzi; Chiara Napoli; Luca Sardo; Gian Paolo Accotto. 2014. "Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection." PLOS ONE 9, no. 2: e89951.

Journal article
Published: 01 January 2014 in Procedia Engineering
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ACS Style

M. Salina; Fabio Giavazzi; E. Ceccarello; Francesco Damin; Marcella Chiari; Marina Ciuffo; Gian Paolo Accotto; M. Buscaglia. Multi-spot, Label-free Detection of Biomarkers in Complex Media by Reflectionless Surfaces. Procedia Engineering 2014, 87, 58 -61.

AMA Style

M. Salina, Fabio Giavazzi, E. Ceccarello, Francesco Damin, Marcella Chiari, Marina Ciuffo, Gian Paolo Accotto, M. Buscaglia. Multi-spot, Label-free Detection of Biomarkers in Complex Media by Reflectionless Surfaces. Procedia Engineering. 2014; 87 ():58-61.

Chicago/Turabian Style

M. Salina; Fabio Giavazzi; E. Ceccarello; Francesco Damin; Marcella Chiari; Marina Ciuffo; Gian Paolo Accotto; M. Buscaglia. 2014. "Multi-spot, Label-free Detection of Biomarkers in Complex Media by Reflectionless Surfaces." Procedia Engineering 87, no. : 58-61.

Journal article
Published: 01 December 2013 in Virus Research
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RNA silencing is a defense mechanism exploited by plants against viruses. Upon infection, viral genomes and their transcripts are processed by Dicer-like (DCL) ribonucleases into viral small interfering RNAs (vsRNAs) of 21-24 nucleotides that further guide silencing of viral transcripts. To get an insight into the molecular interaction between tomato and the monopartite phloem-limited begomovirus tomato yellow leaf curl Sardinia virus (TYLCSV), a pathogen inducing a devastating disease of tomato in the Mediterranean region, we characterized by deep sequencing the vsRNA population in virus-infected tomato plants, using a Solexa/Illumina platform. TYLCSV-sRNAs spanned the entire viral genome but were discontinuously distributed throughout it, with a prevalence from the transcribed regions. TYLCSV-sRNAs were mainly 21-22 nucleotides in length and their polarity was asymmetrically distributed along the genome. The most abundant vsRNAs originated from a narrow region overlapping the Rep/C4 genes and from a broader region including the end of the V2 and the beginning of the coat protein genes. Deep sequencing results were validated by different hybridization techniques. Comparisons with the data available on vsRNAs for other begomoviruses highlighted both similarities and differences. Host-derived RNA species cross-reacting with a portion of the viral genome corresponding to the most abundant vsRNAs hotspot were detected. Bioinformatics analyses were carried out to investigate the nature of these host molecules.

ACS Style

Laura Miozzi; Vitantonio Pantaleo; József Burgyán; Gian Paolo Accotto; Emanuela Noris. Analysis of small RNAs derived from tomato yellow leaf curl Sardinia virus reveals a cross reaction between the major viral hotspot and the plant host genome. Virus Research 2013, 178, 287 -296.

AMA Style

Laura Miozzi, Vitantonio Pantaleo, József Burgyán, Gian Paolo Accotto, Emanuela Noris. Analysis of small RNAs derived from tomato yellow leaf curl Sardinia virus reveals a cross reaction between the major viral hotspot and the plant host genome. Virus Research. 2013; 178 (2):287-296.

Chicago/Turabian Style

Laura Miozzi; Vitantonio Pantaleo; József Burgyán; Gian Paolo Accotto; Emanuela Noris. 2013. "Analysis of small RNAs derived from tomato yellow leaf curl Sardinia virus reveals a cross reaction between the major viral hotspot and the plant host genome." Virus Research 178, no. 2: 287-296.

Journal article
Published: 27 September 2013 in Mycorrhiza
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The arbuscular mycorrhizal (AM) symbiosis is considered a natural instrument to improve plant health and productivity since mycorrhizal plants often show higher tolerance to abiotic and biotic stresses. However, the impact of the AM symbiosis on infection by viral pathogens is still largely uncertain and little explored. In the present study, tomato plants were grown under controlled conditions and inoculated with the AM fungus Funneliformis mosseae. Once the mycorrhizal colonization had developed, plants were inoculated with the Tomato yellow leaf curl Sardinia virus (TYLCSV), a geminivirus causing one of the most serious viral diseases of tomatoes in Mediterranean areas. Biological conditions consisted of control plants (C), TYLCSV-infected plants (V), mycorrhizal plants (M), and TYLCSV-infected mycorrhizal plants (MV). At the time of analysis, the level of mycorrhiza development and the expression profiles of mycorrhiza-responsive selected genes were not significantly modified by virus infection, thus indicating that the AM symbiosis was unaffected by the presence and spread of the virus. Viral symptoms were milder, and both shoot and root concentrations of viral DNA were lower in MV plants than in V plants. Overall F. mosseae colonization appears to exert a beneficial effect on tomato plants in attenuating the disease caused by TYLCSV.

ACS Style

Giulia Maffei; Laura Miozzi; Valentina Fiorilli; Mara Novero; Luisa Lanfranco; Gian Paolo Accotto. The arbuscular mycorrhizal symbiosis attenuates symptom severity and reduces virus concentration in tomato infected by Tomato yellow leaf curl Sardinia virus (TYLCSV). Mycorrhiza 2013, 24, 179 -186.

AMA Style

Giulia Maffei, Laura Miozzi, Valentina Fiorilli, Mara Novero, Luisa Lanfranco, Gian Paolo Accotto. The arbuscular mycorrhizal symbiosis attenuates symptom severity and reduces virus concentration in tomato infected by Tomato yellow leaf curl Sardinia virus (TYLCSV). Mycorrhiza. 2013; 24 (3):179-186.

Chicago/Turabian Style

Giulia Maffei; Laura Miozzi; Valentina Fiorilli; Mara Novero; Luisa Lanfranco; Gian Paolo Accotto. 2013. "The arbuscular mycorrhizal symbiosis attenuates symptom severity and reduces virus concentration in tomato infected by Tomato yellow leaf curl Sardinia virus (TYLCSV)." Mycorrhiza 24, no. 3: 179-186.

Journal article
Published: 20 July 2013 in The Plant Journal
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Tomato line 30.4 was obtained engineering the nucleocapsid (N) gene of tomato spotted wilt virus into plant genome, and immunity to tomato spotted wilt virus infection of its self‐pollinated homozygous progeny was observed. Despite the presence of a high amount of transgenic transcripts, transgenic proteins have not been detected, suggesting a mechanism of resistance mediated by RNA. In the present study, we identify post‐transcriptional gene silencing as the main mechanism of resistance, which is able to spread systemically through grafting, and show that the line 30.4 resistant plants produce both 24 and 21–22 nt N‐gene specific siRNA classes. The transgenic locus in chromosome 4 shows complex multiple insertions of four T‐DNA copies in various orientations, all with 3′ end deletions in the terminator and part of the N gene. However, for three of them, polyadenylated transcripts are produced, due to flanking tomato genome sequences acting as alternative terminators. Interestingly, starting at the fifth generation after the transformation event, some individual plants show a tomato spotted wilt virus‐susceptible phenotype. The change is associated with the disappearance of transgene‐specific transcripts and siRNAs, and with hyper‐methylation of the transgene, which proceeds gradually through the generations. Once it reaches a critical threshold, the shift from post‐transcriptional gene silencing to transcriptional silencing of the transgene eliminates the previously well established virus resistance.

ACS Style

Marco Catoni; Alessandra Lucioli; Paula Doblas-Ibáñez; Gian Paolo Accotto; Annamaria Vaira. From immunity to susceptibility: virus resistance induced in tomato by a silenced transgene is lost as TGS overcomes PTGS. The Plant Journal 2013, 75, 941 -953.

AMA Style

Marco Catoni, Alessandra Lucioli, Paula Doblas-Ibáñez, Gian Paolo Accotto, Annamaria Vaira. From immunity to susceptibility: virus resistance induced in tomato by a silenced transgene is lost as TGS overcomes PTGS. The Plant Journal. 2013; 75 (6):941-953.

Chicago/Turabian Style

Marco Catoni; Alessandra Lucioli; Paula Doblas-Ibáñez; Gian Paolo Accotto; Annamaria Vaira. 2013. "From immunity to susceptibility: virus resistance induced in tomato by a silenced transgene is lost as TGS overcomes PTGS." The Plant Journal 75, no. 6: 941-953.

Journal article
Published: 06 December 2012 in Annals of Applied Biology
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ACS Style

R. Soleimani; S. Matic; H. Taheri; S. A. A. Behjatnia; M. Vecchiati; K. Izadpanah; Gian Paolo Accotto. The unconventional geminivirusBeet curly top Iran virus: satisfying Koch's postulates and determining vector and host range. Annals of Applied Biology 2012, 162, 174 -181.

AMA Style

R. Soleimani, S. Matic, H. Taheri, S. A. A. Behjatnia, M. Vecchiati, K. Izadpanah, Gian Paolo Accotto. The unconventional geminivirusBeet curly top Iran virus: satisfying Koch's postulates and determining vector and host range. Annals of Applied Biology. 2012; 162 (2):174-181.

Chicago/Turabian Style

R. Soleimani; S. Matic; H. Taheri; S. A. A. Behjatnia; M. Vecchiati; K. Izadpanah; Gian Paolo Accotto. 2012. "The unconventional geminivirusBeet curly top Iran virus: satisfying Koch's postulates and determining vector and host range." Annals of Applied Biology 162, no. 2: 174-181.