Masson pine is an important afforestation species in southern China, where seasonal drought is common. The present study focused on the effects of Suillus placidus, an ectomycorrhizal fungus, inoculation on the growth and physiological and biochemical performance of masson pine seedlings under four different watering treatments (well-watered, mild drought, moderate drought, and severe drought) to evaluate the symbiotic relationship between S. placidus and masson pine seedlings. Ectomycorrhizal-inoculated (ECM) and non-inoculated (NM) seedlings were grown in pots and maintained for 60 days using the weighing method. Results showed that seedlings’ growth, dry weight, RWC, chlorophyll content, PSII efficiency, and photosynthesis decreased as drought stress intensified in both ECM and NM plants. This suggests that drought stress significantly limits the growth and photosynthetic performance of masson pine seedlings. Nevertheless, increased A_n/g_s and proline contents in both NM and ECM prevented oxidative damage caused by drought stress. In addition, increased peroxidase (POD) activity is an essential defense mechanism of ECM seedling under drought stress. Compared with NM, ECM seedlings showed faster growth, higher RWC, and photosynthetic performance, and lower lipid peroxidation in cell membranes under drought stress, as indicated by higher POD activity and lower proline and malondialdehyde (MDA). Our experiment found that S. placidus inoculation can enhance the drought resistance of masson pine seedlings by increasing antioxidant enzyme activity, water use efficiency, and proline content, thereby enhancing growth under water-deficiency conditions. S. placidus can be used to cultivate high-quality seedlings and improve their survival in regions that experience seasonal droughts.

Studies on the dynamics of non-structural carbohydrates (NSCs) play an important role in understanding the mechanisms of plant responses to drought stress. The objective of this study was to assess the influence of ectomycorrhizal fungi (ECMF) on the content and distribution of NSCs in Pinus massoniana seedlings under different drought intensities and to further explore the possible mechanism by which ECMF enhances the stress resistance of host plants. We conducted a pot experiment using P. massoniana seedlings that were inoculated (M) or non-inoculated (NM) with Suillus luteus (Sl) under well-watered, moderate, and severe drought stress conditions. The results showed that drought significantly reduced the photosynthetic capacity of P. massoniana seedlings and inhibited their growth rate. P. massoniana could respond to different degrees of drought stress by increasing the accumulation of NSCs and increasing WUE. However, compared with well-watered treatment, NSCs consumption began to appear in the roots of NM due to the decrease in starch content under severe drought, whereas NSCs content in M seedlings was higher than that in the well-watered treatment, showing that the ability to maintain C balance was higher in M seedlings. Compared with NM, inoculation with Sl increased the growth rate and biomass of roots, stems, and leaves under moderate and severe drought. In addition, Sl can also improve the gas exchange parameters (net photosynthetic rate, transpiration rate, intercellular CO₂ concentration and stomatal conductance) of P. massoniana seedlings compared with NM seedlings, which was conducive to the hydraulic regulation of seedlings and improved their C fixation capacity. Meanwhile, the content of NSCs in M seedlings was higher. Moreover, the soluble sugar content and SS/St ratio of leaves, roots, and whole plants were higher under drought stress after Sl inoculation, indicating that Sl could also change the C distribution mode, regulate more soluble sugar to respond to drought stress, which was conducive to improving the osmotic adjustment ability of seedlings, and providing more available C sources for plant growth and defense. Overall, inoculation with Sl could enhance the drought resistance of seedlings and promote their growth under drought stress by improving NSCs storage, increasing soluble sugar distribution, and improving the plant water balance of P. massoniana seedlings.

Pruning is an important technique in culturing good knot-free timber. However, to make more accurate pruning plans, it is necessary to consider the growing status of trees and set reasonable pruning intensities based on this. In a seven-year-old Pinus massoniana Lamb. plantation, we carried out pruning twice with a time interval of two years. The treatments included one unpruned treatment (CK) and five pruned treatments (from P1 to P5, representing the live branch height being kept at 68%, 55%, 60%, 55% and 45% of the tree height). CK, P1 and P2 were conducted in the first pruning in March 2019, and the remaining treatments were conducted in the second pruning in January 2021. The growth investigations were carried out in March 2019, December 2019, December 2020 and December 2021. Another investigation was carried out only for the measurement of live branch heights and crown widths in January 2021 just after the second pruning. The results showed that pruning resulted in a 15.08% to 60.62% increase in diameter growth and an 10.28% to 29.87% increase in volume growth. The stem form was also improved. Significant differences were recorded in live branch heights after green pruning but gradually recovered to the same level in two years by branch senescence. Pruning also resulted in a faster extension of the crown width with an enlarged growing space by the removal of green branches. We highlighted that trees with different growth statuses responded differently to pruning intensities: trees of weak growth statuses grew better under a light pruning intensity, while those with a strong growth status grew better under a severe pruning intensity. Overall, for the seven-year-old young mason pine plantation, keeping four rounds of branches in trees with diameters of less than 8 cm and keeping three rounds of branches in trees with diameters of more than 8 cm were appropriate measures.