Atriplex taltalensis is an endangered shrub endemic to northern Chile. Sparse populations of this species can be found in areas with extreme edaphoclimatic conditions in the Atacama desert, and its seeds have a poor germination rate. Consequently, mass-cultivating it can be challenging. In this study, the vegetative propagation mechanisms of A. taltalensis were evaluated using an in vitro culture to aid in their conservation. A. taltalensis shoot explants were treated with two phytoregulators, indole-3-butyric acid (IBA) and 6-benzylaminopurine (BAP), to assess the morphogenic responses and their effects on the propagation of the species, based on shoot multiplication, elongation, and rooting, through subculturing. During multiplication, the treatment with IBA alone efficiently promoted explant elongation, lateral root formation, and axillary shoot proliferation, allowing for the rapid development of shoots into whole plants. Alternatively, treatment with IBA and BAP in combination stimulated the proliferation of basal shoots with little elongation and rooting and promoted shoot hyperhydricity at 0.25–1 mg L⁻¹ BAP concentrations. Thus, we conclude that A. taltalensis propagation is viable through in vitro plant tissue culture using a rapid axillary shoot multiplication system, and this method could aid in the conservation of this species through in vitro propagation and rescue programs.