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Post Doctoral Researcher
01 May 2021 - 01 September 2021
Post Doctoral Researcher
01 August 2019 - 01 March 2021
Post Doctoral Researcher
01 April 2017 - 01 April 2019
The deduced amino acid sequences from the complete cDNA coding sequences of three antioxidant enzyme genes (cm sod1 , cm sod2, and cm cat ) demonstrated that phylogenetically the magur catfish ( Clarias magur ) is very much close to other bony fishes with complete conservation of active site residues among piscine, amphibian and mammalian species. The three-dimensional structures of three antioxidant enzyme proteins are very much similar to mammalian counterparts, thereby suggesting the functional similarities of these enzymes. Exposure to ZnO NPs resulted in an oxidative stress as evidenced by an initial sharp rise of intracellular concentrations of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA), but decreased gradually at later stages. The level of glutathione (GSH) also increased gradually in all the tissues examined after an initial decrease. Biochemical and gene expression analyses indicated that the magur catfish has the ability to defend the ZnO NP-induced oxidative stress by inducing the SOD/CAT enzyme system and also the GSH-related enzymes that are mediated through the activation of various antioxidant-related genes both at the transcriptional and translational levels in various tissues. Furthermore, it appeared that the stimulation of NO, as a consequence of induction nos2 gene, under NP-induced oxidative stress serves as a modulator to induce the SOD/CAT system in various tissues of magur catfish as an antioxidant strategy. Thus, it can be contemplated that the magur catfish possesses a very efficient antioxidant defensive mechanisms to defend against the oxidative stress during exposure to ZnO NPs into their natural environment, and also from related cellular damages.
Debaprasad Koner; Bodhisattwa Banerjee; Annu Kumari; Aquisha S. Lanong; Revelbornstar Snaitang; Nirmalendu Saha. Molecular Characterization of Superoxide Dismutase and Catalase Genes, and The Induction of Antioxidant Genes Under The Zinc Oxide Nanoparticle-Induced Oxidative Stress in Air-Breathing Magur Catfish (Clarias Magur). 2021, 1 .
AMA StyleDebaprasad Koner, Bodhisattwa Banerjee, Annu Kumari, Aquisha S. Lanong, Revelbornstar Snaitang, Nirmalendu Saha. Molecular Characterization of Superoxide Dismutase and Catalase Genes, and The Induction of Antioxidant Genes Under The Zinc Oxide Nanoparticle-Induced Oxidative Stress in Air-Breathing Magur Catfish (Clarias Magur). . 2021; ():1.
Chicago/Turabian StyleDebaprasad Koner; Bodhisattwa Banerjee; Annu Kumari; Aquisha S. Lanong; Revelbornstar Snaitang; Nirmalendu Saha. 2021. "Molecular Characterization of Superoxide Dismutase and Catalase Genes, and The Induction of Antioxidant Genes Under The Zinc Oxide Nanoparticle-Induced Oxidative Stress in Air-Breathing Magur Catfish (Clarias Magur)." , no. : 1.
Living organisms repeatedly encounter stressful events and apply various strategies to survive. Polyamines are omnipresent bioactive molecules with multiple functions. Their transient synthesis, inducible by numerous stressful stimuli, is termed the polyamine stress response. Animals developed evolutionary-conserved strategies to cope with stresses. The urea cycle is an ancient attribute that deals with ammonia excess in terrestrial species. Remarkably, most fish retain the urea cycle genes fully expressed during the early stages of development and silenced in adult animals. Environmental challenges instigate urea synthesis in fish despite substantial energetic costs, which poses a question of the urea cycle's evolutionary significance. Arginase plays a critical role in oxidative stress-dependent reactions being the final urea cycle' enzyme. Its unique subcellular localization, high inducibility, several regulation levels provide a supreme ability to control the polyamine synthesis rate. Of note, oxidative stress instigates the arginase-1 activity in mammals. Arginase is also dysregulated in aging organisms' brain and muscle tissues, indicating its role in the pathogenesis of age-associated diseases. We designed a study to investigate the levels of the urea cycle and polyamine synthesis-related enzymes in a fish model of acute hypoxia. We evidence synchronized elevation of arginase-2 and ornithine decarboxylase following oxidative stress in adult fish and aging animals that underlines the specific function of arginase-2 in fish. Moreover, we demonstrate oxidative stress-associated polyamine synthesis' induction and urea cycle' arrest in adult fish. The subcellular arginase localization found in the fish seems to correspond to its possible evolutionary roles.
Bodhisattwa Banerjee; Iryna Khrystoforova; Baruh Polis; David Karasik. Acute Hypoxia Elevates Arginase 2 and Induces Polyamine Stress Response in Zebrafish via Evolutionary-conserved Mechanism. 2021, 1 .
AMA StyleBodhisattwa Banerjee, Iryna Khrystoforova, Baruh Polis, David Karasik. Acute Hypoxia Elevates Arginase 2 and Induces Polyamine Stress Response in Zebrafish via Evolutionary-conserved Mechanism. . 2021; ():1.
Chicago/Turabian StyleBodhisattwa Banerjee; Iryna Khrystoforova; Baruh Polis; David Karasik. 2021. "Acute Hypoxia Elevates Arginase 2 and Induces Polyamine Stress Response in Zebrafish via Evolutionary-conserved Mechanism." , no. : 1.
The recently emerged SARS-CoV-2 virus is responsible for the ongoing COVID-19 pandemic that has rapidly developed into a global public health threat. Patients severely affected with COVID-19 present distinct clinical features, including acute respiratory disorder, neutrophilia, cytokine storm, and sepsis. In addition, multiple pro-inflammatory cytokines are found in the plasma of such patients. Transcriptome sequencing of different specimens obtained from patients suffering from severe episodes of COVID-19 shows dynamics in terms of their immune responses. However, those host factors required for SARS-CoV-2 propagation and the underlying molecular mechanisms responsible for dysfunctional immune responses during COVID-19 infection remain elusive. In the present study, we analyzed the mRNA-long non-coding RNA (lncRNA) co-expression network derived from publicly available SARS-CoV-2-infected transcriptome data of human lung epithelial cell lines and bronchoalveolar lavage fluid (BALF) from COVID-19 patients. Through co-expression network analysis, we identified four differentially expressed lncRNAs strongly correlated with genes involved in various immune-related pathways crucial for cytokine signaling. Our findings suggest that the aberrant expression of these four lncRNAs can be associated with cytokine storms and anti-viral responses during severe SARS-CoV-2 infection of the lungs. Thus, the present study uncovers molecular interactions behind the cytokine storm activation potentially responsible for hyper-inflammatory responses in critical COVID-19 patients.
Sumit Mukherjee; Bodhisattwa Banerjee; David Karasik; Milana Frenkel-Morgenstern. mRNA-lncRNA Co-Expression Network Analysis Reveals the Role of lncRNAs in Immune Dysfunction during Severe SARS-CoV-2 Infection. Viruses 2021, 13, 402 .
AMA StyleSumit Mukherjee, Bodhisattwa Banerjee, David Karasik, Milana Frenkel-Morgenstern. mRNA-lncRNA Co-Expression Network Analysis Reveals the Role of lncRNAs in Immune Dysfunction during Severe SARS-CoV-2 Infection. Viruses. 2021; 13 (3):402.
Chicago/Turabian StyleSumit Mukherjee; Bodhisattwa Banerjee; David Karasik; Milana Frenkel-Morgenstern. 2021. "mRNA-lncRNA Co-Expression Network Analysis Reveals the Role of lncRNAs in Immune Dysfunction during Severe SARS-CoV-2 Infection." Viruses 13, no. 3: 402.
Long non-coding RNAs (lncRNAs) are the master regulators of numerous biological processes. Hypoxia causes oxidative stress with severe and detrimental effects on brain function and acts as a critical initiating factor in the pathogenesis of Alzheimer's disease (AD). From the RNA-Seq in the forebrain (Fb), midbrain (Mb), and hindbrain (Hb) regions of hypoxic and normoxic zebrafish, we identified novel lncRNAs, whose potential cis targets showed involvement in neuronal development and differentiation pathways. Under hypoxia, several lncRNAs and mRNAs were differentially expressed. Co-expression studies indicated that the Fb and Hb regions' potential lncRNA target genes were involved in the AD pathogenesis. In contrast, those in Mb (cry1b, per1a, cipca) was responsible for regulating circadian rhythm. We identified specific lncRNAs present in the syntenic regions between zebrafish and humans, possibly functionally conserved. We thus identified several conserved lncRNAs as the probable regulators of AD genes (adrb3b, cav1, stat3, bace2, apoeb, psen1, s100b).
Bodhisattwa Banerjee; Debaprasad Koner; David Karasik; Nirmalendu Saha. Genome-wide identification of novel long non-coding RNAs and their possible roles in hypoxic zebrafish brain. Genomics 2020, 113, 29 -43.
AMA StyleBodhisattwa Banerjee, Debaprasad Koner, David Karasik, Nirmalendu Saha. Genome-wide identification of novel long non-coding RNAs and their possible roles in hypoxic zebrafish brain. Genomics. 2020; 113 (1):29-43.
Chicago/Turabian StyleBodhisattwa Banerjee; Debaprasad Koner; David Karasik; Nirmalendu Saha. 2020. "Genome-wide identification of novel long non-coding RNAs and their possible roles in hypoxic zebrafish brain." Genomics 113, no. 1: 29-43.
Long non-coding RNAs (lncRNAs) are the master regulators of numerous biological processes. Hypoxia causes oxidative stress with severe and detrimental effects on brain function and acts as a critical initiating factor in the pathogenesis of Alzheimer’s disease (AD). However, no data are available on the regulatory roles of lncRNAs under hypoxia in the zebrafish brain. From the RNA-Seq in the forebrain (Fb), midbrain (Mb), and hindbrain (Hb) regions of hypoxic and normoxic zebrafish, we identified 8114, 7775, and 7816 novel lncRNA transcripts in the Fb, Mb, and Hb regions, respectively. In the hypoxic fish 357, 553, and 786 novel lncRNA genes were differentially expressed in the Fb, Mb, and Hb regions, respectively. Co-expression network analysis identified a series of lncRNA-mRNA pairs in all the regions, which were enriched for AD. In conclusion, functional annotation of lncRNAs induced by hypoxia provided knowledge on potential regulators for genes participating in AD pathogenesis.
Bodhisattwa Banerjee; Debaprasad Koner; David Karasik; Nirmalendu Saha. Genome-wide identification of novel long non-coding RNAs and their possible roles in hypoxic zebrafish brain. 2020, 1 .
AMA StyleBodhisattwa Banerjee, Debaprasad Koner, David Karasik, Nirmalendu Saha. Genome-wide identification of novel long non-coding RNAs and their possible roles in hypoxic zebrafish brain. . 2020; ():1.
Chicago/Turabian StyleBodhisattwa Banerjee; Debaprasad Koner; David Karasik; Nirmalendu Saha. 2020. "Genome-wide identification of novel long non-coding RNAs and their possible roles in hypoxic zebrafish brain." , no. : 1.
The air-breathing magur catfish (Clarias magur) is a potential ureogenic teleost because of its functional ornithine-urea cycle (OUC), unlike typical freshwater teleosts. The ability to convert ammonia waste to urea was a significant step towards land-based life forms from aquatic predecessors. Here we investigated the molecular characterization of some OUC genes and the molecular basis of stimulation of ureogenesis via the OUC in magur catfish. The deduced amino acid sequences from the complete cDNA coding sequences of ornithine transcarbamyolase, argininosuccinate synthase, and argininosuccinate lyase indicated that phylogenetically magur catfish is very close to other ureogenic catfishes. Ammonia exposure led to a significant induction of major OUC genes and the gene products in hepatic and in certain non-hepatic tissues of magur catfish. Hence, it is reasonable to assume that the induction of ureogenesis in magur catfish under hyper-ammonia stress is mediated through the activation of OUC genes as an adaptational strategy.
Bodhisattwa Banerjee; Debaprasad Koner; Rubaiya Hasan; Nirmalendu Saha. Molecular characterization and ornithine-urea cycle genes expression in air-breathing magur catfish (Clarias magur) during exposure to high external ammonia. Genomics 2019, 112, 2247 -2260.
AMA StyleBodhisattwa Banerjee, Debaprasad Koner, Rubaiya Hasan, Nirmalendu Saha. Molecular characterization and ornithine-urea cycle genes expression in air-breathing magur catfish (Clarias magur) during exposure to high external ammonia. Genomics. 2019; 112 (3):2247-2260.
Chicago/Turabian StyleBodhisattwa Banerjee; Debaprasad Koner; Rubaiya Hasan; Nirmalendu Saha. 2019. "Molecular characterization and ornithine-urea cycle genes expression in air-breathing magur catfish (Clarias magur) during exposure to high external ammonia." Genomics 112, no. 3: 2247-2260.
Two thio-urea based bifunctional probes (H2L1-S and H2L2-S) have been designed and fabricated for the colorimetric and fluorometric detection of mercury(II) and acetate ions respectively in DMSO media. Both the H2L1-S and H2L2-S were fully characterized by various spectroscopic techniques and the solid-state structure of H2L1-S was determined via single-crystal X-ray diffraction. The high sensitivity and selectivity of the probes for Hg2+ were achieved by the Hg2+ promoted desulfurization reactions utilizing the high thiophilic nature of mercury and was monitored by UV–vis spectroscopy. Most interestingly the desulfurized product was isolated and characterized by ESI-MS and single crystal X-ray diffraction. The probes H2L1-S and H2L2-S were found to be highly selective towards acetate ion (AcO-) in fluorescence channel. These probes showed a distinct fluorescence enhancement only in the presence of AcO− at 493 nm due to the inhibition of photo induced electron transfer (PET). Finally, the cytotoxicity study was carried out for biological application. The probe showed minimum cytotoxicity and was suitable for intracellular acetate ion imaging.
Sumit Kumar Patra; Bhaskar Sen; Sanjoy Kumar Sheet; Bodhisattwa Banerjee; Nirmalendu Saha; Snehadrinarayan Khatua. Single molecular dual analyte thio-urea based probes for colorimetric Hg2+ and fluorometric AcO− detection and its’ application in bioimaging. Inorganica Chimica Acta 2019, 492, 119 -130.
AMA StyleSumit Kumar Patra, Bhaskar Sen, Sanjoy Kumar Sheet, Bodhisattwa Banerjee, Nirmalendu Saha, Snehadrinarayan Khatua. Single molecular dual analyte thio-urea based probes for colorimetric Hg2+ and fluorometric AcO− detection and its’ application in bioimaging. Inorganica Chimica Acta. 2019; 492 ():119-130.
Chicago/Turabian StyleSumit Kumar Patra; Bhaskar Sen; Sanjoy Kumar Sheet; Bodhisattwa Banerjee; Nirmalendu Saha; Snehadrinarayan Khatua. 2019. "Single molecular dual analyte thio-urea based probes for colorimetric Hg2+ and fluorometric AcO− detection and its’ application in bioimaging." Inorganica Chimica Acta 492, no. : 119-130.
The facultative air-breathing magur catfish (Clarias magur) frequently face different environmental challenges, such as hyper-ammonia, and desiccation stresses in their natural habitats. All these stresses lead to higher accumulation of body ammonia, thereby causing various harmful effects to the fish due to its toxicity. Nonetheless, the mechanisms underlying ammonia-induced toxicity is yet not clear. In the present study, we used RNA sequencing and utilized a modified method for de novo assembly of the transcriptome to provide an exhaustive study on the transcriptomic alterations of magur catfish in response to high environmental ammonia (HEA; 25 mM NH4Cl). The final contig assembly produced a total of 311,076 unique transcripts (termed as unigenes) with a GC content of 48.3% and the average length of 599 bp. A considerable number of SSR marker associated with these unigenes were also detected. A total of 279,156 transcripts were successfully annotated by using various databases. Comparative transcriptomic analysis revealed a total of 3453 and 19,455 genes were differentially expressed in the liver and brain tissues, respectively, in ammonia-treated fish compared to the control. Enrichment analysis of the differentially expressed genes (DEGs) showed that several GO and KEGG pathway terms were significantly over-represented. Functional analysis of significantly elevated DEGs demonstrated that ammonia stress tolerance of the magur catfish was associated with quite a few pathways related to immune response, oxidative stress, and apoptosis, as well as few transporter proteins involved with ammonia and urea transport. Both liver and brain tissues showed HEA-mediated oxidative damage with consequent activation of antioxidant machinery. However, elevated ROS levels led to an activation of inflammatory cytokines and thus innate immune response in the liver. Conversely, in the brain ROS-mediated irreversible cell damages activated apoptosis via both p53-Bax-Bcl2 and caspase-mediated pathways. The present study provides a novel understanding of the molecular responses of this air-breathing catfish against the ammonia-induced stressors, which could elucidate the underlying mechanisms of adaptation of this facultative air-breather living under various environmental constraints.
Bodhisattwa Banerjee; Debaprasad Koner; Rubaiya Hasan; Samir Bhattacharya; Nirmalendu Saha. Transcriptome analysis reveals novel insights in air-breathing magur catfish (Clarias magur) in response to high environmental ammonia. Gene 2019, 703, 35 -49.
AMA StyleBodhisattwa Banerjee, Debaprasad Koner, Rubaiya Hasan, Samir Bhattacharya, Nirmalendu Saha. Transcriptome analysis reveals novel insights in air-breathing magur catfish (Clarias magur) in response to high environmental ammonia. Gene. 2019; 703 ():35-49.
Chicago/Turabian StyleBodhisattwa Banerjee; Debaprasad Koner; Rubaiya Hasan; Samir Bhattacharya; Nirmalendu Saha. 2019. "Transcriptome analysis reveals novel insights in air-breathing magur catfish (Clarias magur) in response to high environmental ammonia." Gene 703, no. : 35-49.
The present study investigated the role of taurine in cellular volume regulation of erythrocytes isolated from freshwater air-breathing magur catfish (Clarias magur) under osmotic stress. Exposure of erythrocytes, pre-loaded with or without taurine, to hypotonic medium (− 80 mOsmol/L) led to a significant decrease in taurine level in the erythrocytes due to efflux of taurine through a band 3 transporter protein present in the plasma membrane with a slight increase in cellular volume of erythrocytes by 12–13%, whereas incubation of erythrocytes with hypertonic medium (+ 80 mOsmol/L) with taurine caused a significant uptake of taurine by the erythrocytes through the Na+-dependent pathway but without any loss of taurine from the erythrocytes which was accompanied by a slight decrease in the cellular volume of erythrocytes by 11–12%. Furthermore, a direct correlation between the osmosensitive cellular volume and taurine release could be established in the erythrocytes of magur catfish under hypotonic stress (r = 0.9921). In conclusion, the erythrocytes of air-breathing magur catfish do possess a very efficient taurine-dependent volume regulatory mechanism to resist the changes in cellular volume under anisotonic conditions as a unique adaptational strategy to defend against the osmosensitive changes in cellular volume of erythrocytes.
Bodhisattwa Banerjee; Debaprasad Koner; Priyanka Lal; Suman Kumari; Rubaiya Hasan; Nirmalendu Saha. Role of Taurine in Cellular Volume Regulation in Erythrocytes of Air-Breathing Catfish (Clarias magur) Under Osmotic Stress. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 2019, 89, 1389 -1397.
AMA StyleBodhisattwa Banerjee, Debaprasad Koner, Priyanka Lal, Suman Kumari, Rubaiya Hasan, Nirmalendu Saha. Role of Taurine in Cellular Volume Regulation in Erythrocytes of Air-Breathing Catfish (Clarias magur) Under Osmotic Stress. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 2019; 89 (4):1389-1397.
Chicago/Turabian StyleBodhisattwa Banerjee; Debaprasad Koner; Priyanka Lal; Suman Kumari; Rubaiya Hasan; Nirmalendu Saha. 2019. "Role of Taurine in Cellular Volume Regulation in Erythrocytes of Air-Breathing Catfish (Clarias magur) Under Osmotic Stress." Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 89, no. 4: 1389-1397.
The facultative air-breathing magur catfish (Clarias magur) regularly encounter various environmental challenges including the exposure to nanomaterials discarded as industrial wastes in water bodies. The present investigation aimed at determining the possible ZnO NP-induced oxidative stress and also the antioxidant strategy of nitric oxide (NO), generated endogenously, in primary hepatocytes of magur catfish. Exposure of primary hepatocytes to different concentrations of ZnO NPs (5 and 10 μg/mL) led to a sharp rise of intracellular concentrations of hydrogen peroxide (H2O2) and malondialdehyde (MDA) within 6 h, which decreased gradually at later stages. This phenomenon was accompanied by an initial decrease of superoxide dismutase (SOD) and catalase (CAT) activities, the expression of their corresponding genes and the enzyme protein levels, with a subsequent significant increase of all these parameters at later stages. Most interestingly, exposure to ZnO NPs also stimulated the NO production by the primary hepatocytes as a consequence of induction of inducible nitric oxide synthase (iNOS) activity, higher expression of nos2 gene and iNOS protein. Furthermore, when the NO production by the hepatocytes was inhibited by either aminoguanidine (inhibitor for iNOS) or BAY (inhibitor for NFκB) in the presence of ZnO NPs, the intracellular concentrations of H2O2 and MDA was significantly elevated. This elevation was accompanied by a subsequent decrease of sod and cat genes expression, thereby suggesting that the inhibition of NO production leads to oxidative stress. Thus, it is believed that the magur catfish uses the strategy of stimulation of endogenous NO production by inducing the nos2 gene and simultaneous NO-mediated induction of sod and cat genes to defend against the NP-induced oxidative stress. It is the first report of such NO-mediated antioxidant strategy in any teleost fish to defend against the NP-induced oxidative stress and corresponding cellular damages.
Debaprasad Koner; Bodhisattwa Banerjee; Rubaiya Hasan; Nirmalendu Saha. Antioxidant activity of endogenously produced nitric oxide against the zinc oxide nanoparticle-induced oxidative stress in primary hepatocytes of air-breathing catfish, Clarias magur. Nitric Oxide 2018, 84, 7 -15.
AMA StyleDebaprasad Koner, Bodhisattwa Banerjee, Rubaiya Hasan, Nirmalendu Saha. Antioxidant activity of endogenously produced nitric oxide against the zinc oxide nanoparticle-induced oxidative stress in primary hepatocytes of air-breathing catfish, Clarias magur. Nitric Oxide. 2018; 84 ():7-15.
Chicago/Turabian StyleDebaprasad Koner; Bodhisattwa Banerjee; Rubaiya Hasan; Nirmalendu Saha. 2018. "Antioxidant activity of endogenously produced nitric oxide against the zinc oxide nanoparticle-induced oxidative stress in primary hepatocytes of air-breathing catfish, Clarias magur." Nitric Oxide 84, no. : 7-15.
The present study demonstrates the unique presence of three different gs genes (cmgs01, cmgs02, and cmgs03) in air-breathing ureogenic magur catfish (Clarias magur), which is otherwise reported to be encoded by a single gene in higher vertebrates. Of these three genes, two (cmgs01and cmgs03) were identified as ‘liver’ form, predominantly expressed in liver cells, and the third one as ‘brain’ form (cmgs02), expressed chiefly in brain cells. Molecular characterization studies have revealed conservation of homologous active site residues in all the three gs genes. In silico analysis, accompanied by GS enzyme assay and Western blot analysis of different GS isoforms in different subcellular fractions indicated the mitochondrial localization of cmGS01 and cmGS03 in liver and kidney cells and cytosolic localization of cmGS02 in brain cells. Further, exposure of magur catfish to high external ammonia (HEA; 25 mM NH4Cl) led to a significant induction of multiple gs genes as evidenced by higher expression of different gs mRNAs at variable levels in different tissues. The cmgs01 and cmgs03 mRNA levels elevated significantly in liver, kidney, muscle, and gills, whereas the cmgs02 mRNA level increased considerably in the brain after 14 days of exposure to HEA. These increases in mRNA levels were associated with a significant rise in cmGS01 and cmGS03 proteins in liver, kidney, muscle, and gills, and the cmGS02 protein in the brain after 14 days of exposure to HEA. Therefore, it can be concluded that the unique differential expression of three gs genes and their induction under high ammonia level probably helps in detoxification of ammonia to glutamine and further to urea via the ornithine-urea cycle in ureogenic as well as non-ureogenic tissues of these magur catfish.
Bodhisattwa Banerjee; Debaprasad Koner; Gitalee Bhuyan; Nirmalendu Saha. Differential expression of multiple glutamine synthetase genes in air-breathing magur catfish, Clarias magur and their induction under hyper-ammonia stress. Gene 2018, 671, 85 -95.
AMA StyleBodhisattwa Banerjee, Debaprasad Koner, Gitalee Bhuyan, Nirmalendu Saha. Differential expression of multiple glutamine synthetase genes in air-breathing magur catfish, Clarias magur and their induction under hyper-ammonia stress. Gene. 2018; 671 ():85-95.
Chicago/Turabian StyleBodhisattwa Banerjee; Debaprasad Koner; Gitalee Bhuyan; Nirmalendu Saha. 2018. "Differential expression of multiple glutamine synthetase genes in air-breathing magur catfish, Clarias magur and their induction under hyper-ammonia stress." Gene 671, no. : 85-95.
Arginase (ARG) catalyzes the final step of ornithine-urea cycle (OUC) leading to a conversion of L-arginine to L-ornithine and urea. Several isoforms of ARG have been reported in vertebrates, out of which the two predominant isoforms are the cytosolic ARG1 and the mitochondrial ARG2. The air-breathing walking catfish (Clarias batrachus) is frequently being challenged by different environmental insults such as hyper-ammonia, dehydration and osmotic stresses in their natural habitats throughout the year. The present study investigated the active presence of ARG1 and ARG2 isoforms in hepatocytes along with unique localization of both the isoforms inside the mitochondria, and also their specific expression patterns under hyper-ammonia stress (5mM NHCl) in isolated hepatocytes of walking catfish. Initially, full length sequences of both arg1 and arg2 genes were obtained by RACE-PCR. Studies on molecular characterization demonstrated the presence of all the conserved amino acids required for stability and activity of binuclear metal center in both the isoforms. Phylogenetic analysis of the amino acid sequences of ARG isoforms showed a differentiation of the ARG1 and ARG2 into two distinct clusters with their respective isoforms from other species. Most interestingly, both the isoforms of ARG in hepatocytes were found to be localized inside the mitochondria as evidenced by the presence of mitochondrial target peptide (mTP) in N-terminal of the derived amino acid sequences, and exclusive localization of ARG activity in the mitochondrial fraction. This was additionally confirmed by Western blot analysis of ARGs in mitochondrial and cytosolic fractions, and by immunocytochemical analysis in isolated hepatocytes. Although the possible reasons associated with the presence of both the isoforms of ARGs inside the mitochondria is not clearly understood, perhaps this mitochondrial localization of ARG is functionally advantageous in this catfish for the synthesis of N-acetyl-l-glutamate, the allosteric regulator for the first OUC enzyme, the carbamoyl phosphate synthetase III, and for supplying ornithine required for citrulline synthesis intramitochondrially. Furthermore, the ammonia stress, due to exposure to high external ammonia, led to greater synthesis of urea-N probably as a consequence of induction of ureogenesis, as evidenced by a larger accumulation of urea-N in hepatocytes and higher secretion in culture media parallel to the increased concentration of ammonia-N in hepatocytes. Ammonia stress also led to specific coordinated patterns of induction of both the arg genes in isolated hepatocytes of walking catfish.
Bodhisattwa Banerjee; Debaprasad Koner; Priyanka Lal; Nirmalendu Saha. Unique mitochondrial localization of arginase 1 and 2 in hepatocytes of air-breathing walking catfish, Clarias batrachus and their differential expression patterns under hyper-ammonia stress. Gene 2017, 622, 13 -22.
AMA StyleBodhisattwa Banerjee, Debaprasad Koner, Priyanka Lal, Nirmalendu Saha. Unique mitochondrial localization of arginase 1 and 2 in hepatocytes of air-breathing walking catfish, Clarias batrachus and their differential expression patterns under hyper-ammonia stress. Gene. 2017; 622 ():13-22.
Chicago/Turabian StyleBodhisattwa Banerjee; Debaprasad Koner; Priyanka Lal; Nirmalendu Saha. 2017. "Unique mitochondrial localization of arginase 1 and 2 in hepatocytes of air-breathing walking catfish, Clarias batrachus and their differential expression patterns under hyper-ammonia stress." Gene 622, no. : 13-22.
The obligatory air-breathing mud eel (Monopterus cuchia) is frequently being challenged with high environmental ammonia (HEA) exposure in its natural habitats. The present study investigated the possible induction of heat shock protein 70 and 90 (hsp70, hsc70, hsp90α and hsp90β) genes and more expression of Hsp70 and Hsp90 proteins under ammonia stress in different tissues of the mud eel after exposure to HEA (50 mM NH4Cl) for 14 days. HEA resulted in significant accumulation of toxic ammonia in different body tissues and plasma, which was accompanied with the stimulation of oxidative stress in the mud eel as evidenced by more accumulation of malondialdehyde (MDA) and hydrogen peroxide (H2O2) during exposure to HEA. Further, hyper-ammonia stress led to significant increase in the levels of mRNA transcripts for inducible hsp70 and hsp90α genes and also their translated proteins in different tissues probably as a consequence of induction of hsp70 and hsp90α genes in the mud eel. However, hyper-ammonia stress was neither associated with any significant alterations in the levels of mRNA transcripts for constitutive hsc70 and hsp90β genes nor their translated proteins in any of the tissues studied. More abundance of Hsp70 and Hsp90α proteins might be one of the strategies adopted by the mud eel to defend itself from the ammonia-induced cellular damages under ammonia stress. Further, this is the first report of ammonia-induced induction of hsp70 and hsp90α genes under hyper-ammonia stress in any freshwater air-breathing teleost.
Hnunlalliani Hangzo; Bodhisattwa Banerjee; Shrabani Saha; Nirmalendu Saha. Ammonia stress under high environmental ammonia induces Hsp70 and Hsp90 in the mud eel, Monopterus cuchia. Fish Physiology and Biochemistry 2016, 43, 77 -88.
AMA StyleHnunlalliani Hangzo, Bodhisattwa Banerjee, Shrabani Saha, Nirmalendu Saha. Ammonia stress under high environmental ammonia induces Hsp70 and Hsp90 in the mud eel, Monopterus cuchia. Fish Physiology and Biochemistry. 2016; 43 (1):77-88.
Chicago/Turabian StyleHnunlalliani Hangzo; Bodhisattwa Banerjee; Shrabani Saha; Nirmalendu Saha. 2016. "Ammonia stress under high environmental ammonia induces Hsp70 and Hsp90 in the mud eel, Monopterus cuchia." Fish Physiology and Biochemistry 43, no. 1: 77-88.
Bodhisattwa Banerjee; Gitalee Bhuyan; Nirmalendu Saha. Influence of environmental hypertonicity on the induction of ureogenesis and amino acid metabolism in air-breathing walking catfish (Clarias batrachus, Bloch). Indian journal of experimental biology 2014, 52, 1 .
AMA StyleBodhisattwa Banerjee, Gitalee Bhuyan, Nirmalendu Saha. Influence of environmental hypertonicity on the induction of ureogenesis and amino acid metabolism in air-breathing walking catfish (Clarias batrachus, Bloch). Indian journal of experimental biology. 2014; 52 (7):1.
Chicago/Turabian StyleBodhisattwa Banerjee; Gitalee Bhuyan; Nirmalendu Saha. 2014. "Influence of environmental hypertonicity on the induction of ureogenesis and amino acid metabolism in air-breathing walking catfish (Clarias batrachus, Bloch)." Indian journal of experimental biology 52, no. 7: 1.
The air-breathing singhi catfish (Heteropneustes fossilis) is frequently being challenged by different environmental insults such as hyper-ammonia, dehydration and osmotic stresses in their natural habitats throughout the year. The present study investigated the effect of hyperosmotic stress, due to exposure to hypertonic environment (300 mM mannitol) for 14 days, on gluconeogenesis in this catfish. In situ exposure to hypertonic environment led to significant stimulation of gluconeogenic fluxes from the perfused liver after 7 days of exposure, followed by further increase after 14 days in presence of three different potential gluconeogenic substrates (lactate, pyruvate and glutamate). Environmental hypertonicity also caused a significant increase of activities of key gluconeogenic enzymes, namely phosphoenolpyruvate carboxykinase, fructose 1, 6-bisphosphatase and glucose 6-phosphatase by about 2-6 fold in liver, and 3-6 fold in kidney tissues. This was accompanied by more abundance of enzyme proteins by about 1.8–3.7 fold and mRNAs by about 2.2–5.2 fold in both the tissues with a maximum increase after 14 days of exposure. Hence, the increase in activities of key gluconeogenic enzymes under hypertonic stress appeared to be as a result of transcriptional regulation of genes. Immunocytochemical analysis further confirmed the tissue specific localized expression of these enzymes in both the tissues with the possibility of expressing more in the same localized places. The induction of gluconeogenesis during exposure to environmental hypertonicity possibly occurs as a consequence of changes in hydration status/cell volume of different cell types. Thus, these adaptational strategies related to gluconeogenesis that are observed in this catfish under hypertonic stress probably help in maintaining glucose homeostasis and also for a proper energy supply to support metabolic demands mainly for ion transport and other altered metabolic processes under various environmental hypertonic stress-related insults.
Manas Das; Bodhisattwa Banerjee; Mahua G. Choudhury; Nirmalendu Saha. Environmental Hypertonicity Causes Induction of Gluconeogenesis in the Air-Breathing Singhi Catfish, Heteropneustes fossilis. PLOS ONE 2013, 8, e85535 .
AMA StyleManas Das, Bodhisattwa Banerjee, Mahua G. Choudhury, Nirmalendu Saha. Environmental Hypertonicity Causes Induction of Gluconeogenesis in the Air-Breathing Singhi Catfish, Heteropneustes fossilis. PLOS ONE. 2013; 8 (12):e85535.
Chicago/Turabian StyleManas Das; Bodhisattwa Banerjee; Mahua G. Choudhury; Nirmalendu Saha. 2013. "Environmental Hypertonicity Causes Induction of Gluconeogenesis in the Air-Breathing Singhi Catfish, Heteropneustes fossilis." PLOS ONE 8, no. 12: e85535.