As a tool for modifying the genome, gene editing technology has developed rapidly in recent years, especially in the past two years. With the emergence of new gene editing technologies, such as transposon editing tools, numerous advancements have been made including precise editing of the genome, double base editing, and pilot editing. This report focuses on the development of gene editing tools in recent years, elaborates the progress made in classic editing tools, base editor and other new editing tools, and provides insights into challenges and opportunities.

Bolbolaimus major sp. nov. is described from Rizhao coast along the Yellow Sea. The new species is characterized by relatively large body size, cuticle strongly annulated, six outer labial sensilla setiform, four cephalic setae long, buccal cavity wide with one large dorsal tooth and two small subventral teeth, amphidial fovea unispiral shaped oval in males and circular in females, spicules curved with swollen proximal end, gubernaculum with anterior-hooked dorso-caudal apophysis, and tail conical with short spinneret. A phylogenetic analysis of maximum-likelihood and Bayesin inference based on rDNA sequences confirms the taxonomic position of Bolbolaimus major sp. nov. within the family Microlaimidae. Tree topology in Microlaimidae shows genus Microlaimus as polyphyletic group and genera in Microlaimidae are closely related.

Two new free-living nematode species, Ingenia major sp. nov. and Linhystera filiformis sp. nov., are described from the Yellow China Sea. Ingenia major sp. nov. is characterized by a large body size, six outer labial setae articulated, buccal cavity barrel-shaped with three teeth, amphidial fovea small and unispiral, spicules thin and almost straight with a pair of ventro-laterally directed teeth hooked distally, gubernaculum kidney-shaped with triangular cuticularized projection, tail conical and with swollen-terminus. Relationship between Ingenia and Bathylaimus is discussed, and Ingenia communis Gagarin & Thanh, 2007 is transferred as Bathylaimus communis comb. n. based on the buccal cavity shape. Linhystera filiformis sp. nov. is characterized by filiform body, buccal cavity minute and slit-like, six outer labial setae and four cephalic setae in the same circle and with the same length, amphidial fovea relatively small and circular, spicules short and sickle-shaped, gubernaculum boat-shaped, tail filiform with three terminal setae. An identification key for valid species of Linhystera is also given.

Two new free-living nematode species, Axonolaimus rushanensis sp. nov. and Trichromadora spheribulba sp. nov. are described from Rushan, Yellow Sea, China. Axonolaimus rushanensis sp. nov. is characterized by two cervical setae, amphidial fovea loop-shaped with rounded outline at the level of buccal cavity, spicules short and arcuate, cuticularised and with straight capitulum, gubernaculum with dorso-caudal apophyses and tail conical. Trichromadora spheribulba sp. nov. is characterized by long and slender body, buccal cavity with one large hollow dorsal tooth and two small subventral teeth, pharynx with an obvious posterior bulb, spicules long and arc-shaped, gubernaculum boat-shaped and swollen in the middle part, seven unequidistant cup-shaped supplements and tail conical. An identification key to valid species of the genus Trichromadora is also given.