Direct-fed microbial feed additives with potential to enhance growth performance, gut health, and immunity have gained considerable popularity in neonatal calf production. Lactobacillus plantarum GB LP-1 (LP) produced by a proprietary fermentation process could be a viable direct-fed microbial feed for neonatal calves. The hypothesis was that feeding LP may ease transitioning from milk replacer (MR) to calf starter (CS) by improving gut health and appetite, while minimizing health challenges from pathogens and stress to improve growth performance. The experimental objective was to evaluate LP in an MR feeding program at 3 inclusion rates. Fifty-one 2- to 5-d-old Holstein bull calves were randomly assigned to 1 of 3 treatments using a randomized complete block design. Treatments were (1) Control (LP0): LP fed at 0 g/d; (2) LP4: LP fed at 4 g/d; and (3) LP8: LP fed at 8 g/d. Calves were fed MR at 0.57 kg/d for 14 d via bucket, which was increased to 0.85 kg/d until 35 d, and were then fed once daily at 0.425 kg/d with weaning after d 42 of the 56-d experiment. Calves were fed at 0630 and 1800 h in equal allotments, with access at all times to free-choice water and a pelleted CS with 25.5% crude protein. Calves demonstrated a linear growth response to increasing LP inclusion rate: calves fed LP8 gained more body weight (33.0, 36.9, and 37.7 kg for LP0, LP4, and LP8, respectively) than calves fed LP0, with calves fed LP4 being intermediate and similar. The 0-to-42-d (MR feeding phase) average daily gain (ADG; 562.9, 595.9, and 655.7 g/d) and 0-to-56-d ADG (588.6, 658.4, and 673.0 g/d) demonstrated linear responses, with calves fed LP8 having greater ADG than calves fed LP0, and calves fed LP4 being intermediate and similar. Total CS intake was similar among calves fed all treatments (66.3, 69.0, and 72.5 kg/56 d), which resulted in a quadratic response in feed efficiency (0.50, 0.53, and 0.52 kg of gain/kg of dry matter) for calves fed LP4 compared with calves fed LP0, with calves fed LP8 being intermediate and similar. Fecal scores improved linearly with increasing LP inclusion rate. These data demonstrate that feeding Lactobacillus plantarum GB LP-1 to neonatal calves improves gut health to increase growth performance at 4 and 8 g/d, while feed efficiency was greatest at 4 g/d.

Shifting ruminal fermentation via feeding a blend of oregano (Organum vulgare L.) essential oils and Co-lactate (EOC; Rum-A-Fresh, Ralco, Inc. Marshall, MN) could improve lamb growth and carcass performance. Eighteen Suffolk × Little Han Tail F1 male lambs (20.3 ± 0.23 kg BW and approximately 3 months old) were randomly assigned using a completely random design to one of three treatments. Treatments were (1) EOC0: basal ration without EOC, (2) EOC4: basal ration plus 4 g/d EOC, and (3) EOC7: basal ration plus 7 g/d EOC. Initial and 24 d BW was similar (P > 0.10), but at 48 and 72 d, lambs fed EOC7 demonstrated greater (P = 0.01) BW compared with EOC0 fed lambs, while lambs fed EOC4 were intermediate and similar (P > 0.05). Average daily gains (ADGs) for 0-24 and 0-72 d were greater (P < 0.05) for lambs fed EOC4 and EOC7 compared with lambs fed EOC0, while DM intake was similar (P > 0.10). Feed conversions for 0-24 d were improved (P < 0.02) for lambs fed EOC4 and EOC7 compared with lambs fed EOC0. However, 0-72-d feed conversions were greater (P < 0.01) for lambs fed EOC7 compared to lambs fed EOC0, with lambs fed EOC4 being intermediate and similar (P > 0.05). DM, NDF, and ADF digestibilities were similar (P > 0.10) among treatments, while CP digestibility was greater (P < 0.01) for lambs fed EOC4 and EOC7 compared with lambs fed EOC0. Carcass weight and dressing percentages were improved (P < 0.01) for lambs fed EOC7 compared with lambs fed EOC0 and EOC4. Head width was greater (P > 0.01) for lambs fed EOC7 compared with lambs fed EOC0 and EOC4, while rump width was greater (P > 0.01) for lambs fed EOC4 and EOC7 compared with lambs fed EOC0. Plasma triglyceride and cholesterol concentrations were lower (P < 0.05) for lambs fed EOC4 and EOC7 compared with lambs fed EOC0, while albumin, total serum protein, and glucose concentrations were greater (P < 0.05) for lambs fed EOC4 and EOC7 compared with lambs fed EOC0. Feeding an EOC blend as an alternative antibiotic growth promoter at 4 and 7 g/d linearly improved lamb growth performance, feed conversions, frame growth, carcass weights, dressing percentages, and immunity.

The dairy sector contributes to global warming through enteric methane (CH4) emissions. Methane is also a loss of energy to the ruminant. Several studies have developed CH4 prediction models to assess mitigation strategies to reduce emissions. However, the majority of these models have low predictive ability or require numerous inputs that are often not readily available in commercial dairy operations. In this context, the objective of the present paper was to develop CH4 prediction models by using varying levels of information available at the farm level. The seven complexity levels used the following information: (1) dietary nutrient composition, (2) milk yield and composition, (3) Levels 1 and 2, (4) Level 3 plus dry matter intake (DMI), (5) Level 4 plus bodyweight, (6) Level 2 plus DMI, and (7) DMI only. Models were fitted to 489 individual enteric-CH4 measurements from 30 indirect calorimetry studies and evaluated with an independent database comprising 215 treatment means from 62 studies collected from the literature. Within each complexity level, all possible mixed-effect models were fitted and those with the lowest values of Akaike or Bayesian information criteria were selected using lme4 package in R. Models were evaluated using mean square prediction error (MSPE) based statistic, root MSPE (RMSPE) to observation standard deviation ratio, concordance correlation coefficient and Nash–Sutcliffe efficiency methods. All fitted models performed well with an acceptable error estimates (RMSPE as a percentage of observed mean (RMSPE%) = 16–24%), with more than two-thirds of total error originating from random bias. Overall, models with DMI were more accurate (RMSPE% = 16–20%) than those without (RMSPE% = 20–24%). Although the best prediction model (RMSPE% = 16%) was developed using Level 5 information, a model using Level 2 information is recommended for on-farm methane estimates if DMI is not measured. The proposed models offer easy and practical tools to dairy producers for predicting CH4 emissions and evaluating CH4 mitigation strategies.

Cobalt is an essential trace mineral required for ruminal vitamin B₁₂ synthesis, but sources differ in ruminal microbial utilization, i.e., cobalt carbonate is poorly water soluble, whereas acetate and lactate forms are water soluble. Reports comparing organic cobalt lactate to other cobalt salts are lacking. The study objective was to determine if feeding cobalt lactate at two inclusion rates resulted in similar growth performance and tissue cobalt concentrations as the carbonate and acetate forms used in feeds. One hundred Angus cross bred steers weighing 385 ± 20 kg were randomly assigned to one of five treatments. Cattle were fed a basal diet plus: 1) cobalt carbonate to supply cobalt at 30 mg/steer/d, 2) cobalt acetate to supply cobalt at 30 mg/steer/d, 3) cobalt acetate to supply cobalt at 60 mg/steer/d, 4) cobalt lactate to supply cobalt at 30 mg/steer/d, and 5) cobalt lactate to supply cobalt at 60 mg/steer/d. Cattle were fed according to industry standards until body fat deposition was visually deemed to grade USDA Choice, which was 92 and 117 d for each of the 2 blocks, respectively. Steers were harvested and carcass measurements recorded along with sampling of adipose, heart, kidney, liver, and muscle for tissue cobalt concentrations. Three statistical contrasts consisted of: 1: inorganic (cobalt carbonate) vs. organic (cobalt acetate and lactate); 2: cobalt acetate vs. cobalt lactate; and 3: feeding rate of 30 vs. 60 mg/steer/d cobalt. Body weight gains, average daily gains, dry matter intake, and feed conversions were similar (P > 0.10) for steers fed all cobalt sources and feeding rates. Hot carcass weight, yield grade, back fat thickness, and ribeye area were similar (P > 0.10) among steers fed all cobalt sources and inclusion rates. Liver, kidney, muscle, and adipose cobalt concentrations were similar (P > 0.08) for steers fed inorganic vs. organic cobalt sources. Feeding cobalt lactate compared with cobalt acetate did not affect (P > 0.10) liver, kidney, heart, muscle, and adipose tissue cobalt concentrations. Feeding 60 mg/steer/d cobalt compared with 30 mg/steer/d increased (P < 0.01) liver, kidney, heart, and adipose tissue cobalt concentrations, while muscle was a tendency (P < 0.06). The study demonstrated that feeding soluble cobalt lactate, a new cobalt source, resulted in similar growth performance, carcass characteristics, and tissue cobalt concentrations when compared with cobalt acetate and carbonate.

Energy is a key factor regulated by the neuroendocrine system. The objective of this study was to compare growth performance, serum biochemical indices, carcass traits, meat quality, and nutrient composition of lambs fed restricted metabolizable energy (ME) intakes to mimic the seasonal changes of the natural grasslands of northwest China. Nineteen male Dorper × Small Tailed Han lambs were assigned to treatments, control (CON) fed at 1.0 MJ/W^0.75 × d⁻¹ throughout the 180 d study, and restricted ME (RES) fed by sequentially restricting ME every 30 d (0.56, 0.84, 1.0, 0.84, 0.56, and 0.28 MJ/W^0.75 × d⁻¹). Lambs were harvested at the end of the study. Lambs fed the CON diet demonstrated higher (P < 0.05) average daily gain (ADG) compared with RES-fed lambs (158.79 and 57.01 g/d, for CON and RES, respectively), although ADG were lower in each period with the last period being a negative ADG for RES-fed lambs resulting in a loss of body weight. Both treatments demonstrated lower ADG in the last 3 study periods compared with the first 2 periods, which may be explained by cold stress (<−3 °C) increasing the lamb’s energy requirement. Feeding lambs a restricted ME diet resulted in lower (P < 0.05) blood serum glucose, triglycerides, total protein, calcium, phosphorus, immunoglobulin G, immunoglobulin M, and immunoglobulin A concentrations compared with CON-fed lambs, but both treatments were similar (P > 0.05) for cholesterol and high-density lipoprotein-cholesterol. Feeding lambs a restricted ME diet increased (P < 0.05) meat pH, while reducing the cooked meat rate compared with CON-fed lambs. Thus, the carcass yield, rib eye muscle area, and shear force were lower (P < 0.05) for RES-fed lambs compared with CON-fed lambs. The meat water concentration was higher (P < 0.05), whereas the concentrations of dry matter, crude fat, crude protein, and carbohydrate were lower (P < 0.05) for lambs fed a RES ME diet compared to CON-fed lambs. These results demonstrate that sequentially restricting ME intake, as natural grasslands might experience during seasonal forage quality and quantity changes, resulted in a reduction of body weight because lambs were mobilizing body reserves as a source of ME. The reduction in concentrations of key serum nutrients, as well as carcass traits and meat composition, demonstrate the body is responding to the meet the lambs ME requirements, which has implications for both meat quality and the animal’s physiological functions.

Y Cobalt lactate is a highly soluble ruminal Co source. Prior research evaluating higher Co feeding rates demonstrated increased ruminal fiber digestion. Feeding high-forage (>70%) rations to late-lactation dairy cows to enhance income over feed cost could potentially benefit from higher ruminal-soluble Co inclusion rates to enhance ruminal fiber and nutrient digestibility. Twenty-four late-lactation [238 +/- 68.8 d in milk (DIM) and 36.4 +/- 5.4 kg/d milk] Holstein dairy cows (10 primiparous and 14 multiparous) were blocked by milk yield, DIM, and parity, and randomly assigned to 1 of 2 treatments. Treatments were (1) control, CoCO3 total mixed ration (TMR) containing 12.5 mg/cow per day of cobalt (cobalt carbonate), and (2) cobalt lactate (Co-LAC), same CoCO3 TMR but containing an additional 50 mg/cow per day of Co, via a 1% cobalt lactate product (CoMax, Ralco Inc.). The basal TMR was 70% forage [60% alfalfa baleage and 40% corn silage on a dry matter (DM) basis] and 30% of the respective experimental grain mix on a DM basis. Cows were fed the CoCO3 TMR during the 7-d covariate period, followed by 4 wk of data collection when CoCO3 and Co-LAC TMR were fed. Milk production (26.2 and 25.8 kg/d for CoCO3 and Co-LAC, respectively) and dry matter intake (DMI; 22.9 and 23.1 kg/d) were similar for cows fed both Co treatments. Production of milk fat (1.02 and 1.09 kg/d), milk protein (0.87 and 0.91 kg/d), and lactose (1.17 and 1.26 kg/d) were similar for cows fed both treatments. Body weights (684 and 674 kg) were similar for cows fed both treatments. Rumen ammonia concentrations (15.8 and 12.3 mg/dL) were lower for cows fed Co-LAC compared with cows fed CoCO3 (15.8 mg/dL). Ruminal molar acetate concentrations (59.5 and 61.1%) were greater for cows fed Co-LAC compared with cows fed CoCO3. Feeding additional Co as cobalt lactate in the TMR did not enhance lactational performance (milk production, composition, DMI, or body weight changes) when fed a high-forage TMR but altered ruminal fermentation with decreased ruminal ammonia and increased ruminal acetate concentrations.