MicroRNAs (miRNAs) are small (∼21-nucleotides) non-coding RNAs found in plant and animals. MiRNAs function as critical post-transcriptional regulators of gene expression by binding to complementary sequences in their target mRNAs, leading to mRNA destabilization and translational inhibition. Plant miRNAs have some distinct characteristics compared to their animal counterparts, including greater evolutionary conservation and unique miRNA processing methods. The lifecycle of a plant begins with embryogenesis and progresses through seed germination, vegetative growth, reproductive growth, flowering and fruiting, and finally senescence and death. MiRNAs participate in the transformation of plant growth and development and directly monitor progression of these processes and the expression of certain morphological characteristics by regulating transcription factor genes involved in cell growth and differentiation. In woody plants, a large and rapidly increasing number of miRNAs have been identified, but their biological functions are largely unknown. In this review, we summarize the progress of miRNA research in woody plants to date. In particular, we discuss the potential roles of these miRNAs in growth, development, and biotic and abiotic stresses responses in woody plants.

The long-term use of chemical insecticides has caused serious problems of insect resistance and environmental pollution; new insecticides are needed to solve this problem. Cyclosporin A (CsA) is a polypeptide produced by many fungi, which is used to prevent or treat immune rejection during organ transplantation. However, little is known about the utility of CsA as an insecticide. Therefore, this study evaluated the insecticidal activity of CsA using Ostrinia furnacalis as a model. The results demonstrated that CsA was toxic to O. furnacalis with LC₅₀ values of 113.02 μg/g and 198.70 μg/g for newly hatched neonates and newly molted third-instar larvae, respectively. Furthermore, CsA treatment had sublethal effects on the development of O. furnacalis, and significantly reduced the fecundity of adults; this suggests that CsA has great potential to suppress O. furnacalis populations. Further analysis revealed that CsA suppressed calcineurin activity in larvae. CsA had independent or synergistic toxic effects on O. furnacalis when combined with β-cypermethrin, indoxacarb, emamectin benzoate, azadirachtin, and the Bacillus thuringiensis toxin Cry1Ac, which suggests that CsA can help prevent or manage resistance. Our study provides detailed information on the potential of CsA as an insecticide for controlling lepidopterans.

Leaves are essential vegetative organs of plants. Studying the variations in leaf nutrient content and microbial communities of male and female plants at reproductive stages helps us understand allocation and adaptation strategies. This study aimed to determine the nutrient characteristics and microbial differences in the leaves of male and female Idesia polycarpa at reproductive stages. Seven-year-old female and male plants were used as test materials in this experiment. The samples were collected at three stages: flowering (May), fruit matter accumulation (July), and fruit ripening (October). The nitrogen (TN), phosphorus (TP), potassium (TK), carbon (TC), and the pH of the female and male leaves were analyzed. In addition, the leaf microbial diversity and differential metabolites were determined using the Illumina high-throughput sequencing method and the ultra-high performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method at the reproductive developmental stages. This study found that male and female plant leaves had different TN and TK contents over time but no difference in TC and TP content. The significant differences in bacterial diversity between male and female plants and the richness of the fungi of male plants at the flowering and fruit maturity stages were observed. Proteobacteria, Pseudomonadaceae, Ascomycota, and Aspergillus were the dominant bacteria and fungi in the Idesia polycarpa leaves. The presence of microorganisms differed in the two sexes in different periods. Alphaproteobacteria and Sordariomycetes were the indicator groups for male leaves, and Pseudomonas and Sordariomycetes were the indicator groups for female leaves. Significant differences in phenolic acid were found between male and female leaves. A KEGG enrichment analysis revealed that differential metabolites were enriched in metabolic pathways, amino acid biosynthesis, and the nucleotide metabolism. According to a correlation analysis, leaf TK and TP were strongly correlated with endophytic bacteria abundance and differential metabolite composition. This study revealed the changes in substances and microorganisms in the leaves of male and female plants in their reproductive stages. It provides a theoretical basis for developing and utilizing the leaves of Idesia polycarpa and for field management.

The fall armyworm (FAW), Spodoptera frugiperda, causes substantial annual agricultural production losses worldwide due to its resistance to many insecticides. Therefore, new insecticides are urgently needed to more effectively control FAW. Cyclosporin A (CsA) is a secondary metabolite of fungi; little is known about its insecticidal activity, especially for the control of FAW. In this study, we demonstrate that CsA shows excellent insecticidal activity (LC₅₀ = 9.69 μg/g) against FAW through significant suppression of calcineurin (CaN) activity, which is a new target for pest control. Combinations of CsA and indoxacarb, emamectin benzoate, or Vip3Aa showed independent or synergistic toxicity against FAW; however, the combination of CsA and chlorantraniliprole showed no toxicity. Sublethal doses of CsA led to decreases in FAW larval and pupal weight, pupation, emergence, mating rates, adult longevity, extended development of FAW larvae and pupae and the pre-oviposition period of adults, and increases in the proportion of pupal malformation. Importantly, CsA treatment reduced FAW ovarian size and female fecundity, which suggests that it has great potential to suppress FAW colony formation. Taken together, these results indicate that CsA has high potential as an insecticide for controlling FAW.

Idesia polycarpa Maxim is a native dioecious tree from East Asia cultivated for its fruits and as an ornamental plant throughout temperate regions. Given the economic potential, comparative studies on cultivated genotypes are of current interest. This study aims to discover the dynamic changes and potential functions of endogenous hormones in I. polycarpa, as well as the differences in endogenous hormone contents in different growth stages among different I. polycarpa provenances. We used High-Performance Liquid Chromatography (HPLC) to measure and compare the levels of abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellin A3 (GA3), and trans-Zeatin-riboside (tZR) in the leaves, flowers, and fruits of I. polycarpa from various provenances between April and October. Our findings indicated that changes in the ABA and GA3 content of plants from Jiyuan and Tokyo were minimal from April to October. However, the levels of these two hormones in Chengdu plants vary greatly at different stages of development. The peak of IAA content in the three plant materials occurred primarily during the early fruit stage and the fruit expansion stage. The concentration of tZR in the three plant materials varies greatly. Furthermore, we discovered that the contents of endogenous hormones in I. polycarpa leaves, flowers, and fruits from Chengdu provenances were slightly higher than those from Tokyo and Jiyuan provenances. The content of IAA was higher in male flowers than in female flowers, and the content of ABA, GA3, and tZR was higher in female flowers than in male flowers. According to the findings, the contents of these four endogenous hormones in I. polycarpa are primarily determined by the genetic characteristics of the trees and are less affected by cultivation conditions. The gender of I. polycarpa had a great influence on these four endogenous hormones. The findings of this study will provide a theoretical foundation and practical guidance for artificially regulating the flowering and fruiting of I. polycarpa.

Porosity is the key factor in determining the CO₂ capture capacity for porous carbon-based adsorbents, especially narrow micropores of less than 1.0 nm. Unfortunately, this desired feature is still a great challenge to tailor micropores by an effective, low-corrosion, and environmentally friendly activating agent. Herein, we reported a suitable dynamic porogen of CuCl₂ to engineer microporous carbons rich in narrow micropores of <1.0 nm for solving the above problem. The porosity can be easily tuned by varying the concentration of the CuCl₂ porogen. The resultant porous carbons exhibited a multiscale micropore size, high micropore volume, and suitable surface nitrogen doping content, especially high-proportioned ultromicropores of <0.7 nm. As adsorbents for capturing CO₂, the obtained microporous carbons possess satisfactory CO₂ uptake, moderate heat of CO₂ adsorption, reasonable CO₂/N₂ selectivity, and easy regeneration. Our work proposes an alternative way to design porous carbon-based adsorbents for efficiently capturing CO₂ from the postcombustion flue gases. More importantly, this work opens up an almost-zero cost and industrially friendly route to convert biowaste into high-added-value adsorbents for CO₂ capture in an industrial practical application.