At present, huge amounts of data are being produced every second, a situation that will gradually overwhelm current storage technology. DNA is a storage medium that features high storage density and long-term stability and is now considered to be a feasible storage solution. Errors are easily made during the sequencing and synthesis of DNA, however. In order to reduce the error rate, a novel uncorrelated address constrain are reported, and a Damping Multi-Verse Optimizer (DMVO) algorithm is proposed to construct a set of DNA coding, which is used as the non-payload. The DMVO algorithm exchanges objects through black/white holes in order to achieve a stable state and adds damping factors as disturbances. Compared with previous work, the coding set obtained by the DMVO algorithm is larger in size and of higher quality. The results of this study reveal that the size of the DNA storage coding set obtained by the DMVO algorithm increased by 416%, and the variance of the melting temperature decreased by 318%.

With the development of information technology, huge amounts of data are produced at the same time. How to store data efficiently and at low cost has become an urgent problem. DNA is a high-density and persistent medium, making DNA storage a viable solution. In a DNA data storage system, the first consideration is how to encode the data effectively into code words. However, DNA strands are prone to non-specific hybridization during the hybridization reaction process and are prone to errors during synthesis and sequencing. In order to reduce the error rate, a thermodynamic minimum free energy (MFE) constraint is proposed and applied to the construction of coding sets for DNA storage. The Brownian multi-verse optimizer (BMVO) algorithm, based on the Multi-verse optimizer (MVO) algorithm, incorporates the idea of Brownian motion and Nelder-Mead method, and it is used to design a better DNA storage coding set. In addition, compared with previous works, the coding set has been increasing by 4%-50% in size and has better thermodynamic properties. With the improvement of the quality of the DNA coding set, the accuracy of reading and writing and the robustness of the DNA storage system are also enhanced.

The rapid development of information technology has generated substantial data, which urgently requires new storage media and storage methods. DNA, as a storage medium with high density, high durability, and ultra-long storage time characteristics, is promising as a potential solution. However, DNA storage is still in its infancy and suffers from low space utilization of DNA strands, high read coverage, and poor coding coupling. Therefore, in this work, an adaptive coding DNA storage system is proposed to use different coding schemes for different coding region locations, and the method of adaptively generating coding constraint thresholds is used to optimize at the system level to ensure the efficient operation of each link. Images, videos, and PDF files of size 698 KB were stored in DNA using adaptive coding algorithms. The data were sequenced and losslessly decoded into raw data. Compared with previous work, the DNA storage system implemented by adaptive coding proposed in this paper has high storage density and low read coverage, which promotes the development of carbon-based storage systems.

The high density, large capacity, and long-term stability of DNA molecules make them an emerging storage medium that is especially suitable for the long-term storage of large datasets. The DNA sequences used in storage need to consider relevant constraints to avoid nonspecific hybridization reactions, such as the No-runlength constraint, GC-content, and the Hamming distance. In this work, a new nonlinear control parameter strategy and a random opposition-based learning strategy were used to improve the Harris hawks optimization algorithm (for the improved algorithm NOL-HHO) in order to prevent it from falling into local optima. Experimental testing was performed on 23 widely used benchmark functions, and the proposed algorithm was used to obtain better coding lower bounds for DNA storage. The results show that our algorithm can better maintain a smooth transition between exploration and exploitation and has stronger global exploration capabilities as compared with other algorithms. At the same time, the improvement of the lower bound directly affects the storage capacity and code rate, which promotes the further development of DNA storage technology.

DNA Encoding, as a key step in DNA storage, plays an important role in reading and writing accuracy and the storage error rate. However, currently, the encoding efficiency is not high enough and the encoding speed is not fast enough, which limits the performance of DNA storage systems. In this work, a DNA storage encoding system with a graph convolutional network and self-attention (GCNSA) is proposed. The experimental results show that DNA storage code constructed by GCNSA increases by 14.4% on average under the basic constraints, and by 5%-40% under other constraints. The increase of DNA storage codes effectively improves the storage density of 0.7-2.2% in the DNA storage system. The GCNSA predicted more DNA storage codes in less time while ensuring the quality of codes, which lays a foundation for higher read and write efficiency in DNA storage.

Over the past decade, the rapid development of DNA synthesis and sequencing technologies has enabled preliminary use of DNA molecules for digital data storage, overcoming the capacity and persistence bottlenecks of silicon-based storage media. DNA storage has now been fully accomplished in the laboratory through existing biotechnology, which again demonstrates the viability of carbon-based storage media. However, the high cost and latency of data reconstruction pose challenges that hinder the practical implementation of DNA storage beyond the laboratory. In this article, we review existing advanced DNA storage methods, analyze the characteristics and performance of biotechnological approaches at various stages of data writing and reading, and discuss potential factors influencing DNA storage from the perspective of data reconstruction.