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The bacterial strain, EMM-1, was isolated from the rhizosphere of red maize (“Rojo Criollo”) and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production of antimicrobial compounds like 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin, and lectin-like bacteriocins. These antimicrobial compounds are also produced by other fluorescent pseudomonads alike P. protegens. Double-layer agar assay showed that P. protegens EMM-1 inhibited the growth of several multidrug-resistant (MDR) bacteria, especially clinical isolates of the genera Klebsiella and β-hemolytic Streptococcus. This strain also displayed inhibitory effects against diverse fungi, such as Aspergillus, Botrytis, and Fusarium. Besides, a crude extract of inhibitory substances secreted into agar was obtained after the cold-leaching process, and physicochemical characterization was performed. The partially purified inhibitory substances produced by P. protegens EMM-1 inhibited the growth of Streptococcus sp. and Microbacterium sp., but no inhibitory effect was noted for other bacterial or fungal strains. The molecular weight determined after ultrafiltration was between 3 and 10 kDa. The inhibitory activity was thermally stable up to 60°C (but completely lost at 100°C), and the inhibitory activity remained active in a wide pH range (from 3 to 9). After treatment with a protease from Bacillus licheniformis, the inhibitory activity was decreased by 90%, suggesting the presence of proteic natural compounds. All these findings suggested that P. protegens EMM-1 is a potential source of antimicrobials to be used against pathogens for humans and plants.
Catherine Cesa-Luna; Antonino Baez; Alberto Aguayo-Acosta; Roberto Carlos Llano-Villarreal; Víctor Rivelino Juárez-González; Paul Gaytán; María Del Rocío Bustillos-Cristales; América Rivera-Urbalejo; Jesús Muñoz-Rojas; Verónica Quintero-Hernández. Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances. 2020, 15, 1 .
AMA StyleCatherine Cesa-Luna, Antonino Baez, Alberto Aguayo-Acosta, Roberto Carlos Llano-Villarreal, Víctor Rivelino Juárez-González, Paul Gaytán, María Del Rocío Bustillos-Cristales, América Rivera-Urbalejo, Jesús Muñoz-Rojas, Verónica Quintero-Hernández. Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances. . 2020; 15 (10):1.
Chicago/Turabian StyleCatherine Cesa-Luna; Antonino Baez; Alberto Aguayo-Acosta; Roberto Carlos Llano-Villarreal; Víctor Rivelino Juárez-González; Paul Gaytán; María Del Rocío Bustillos-Cristales; América Rivera-Urbalejo; Jesús Muñoz-Rojas; Verónica Quintero-Hernández. 2020. "Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances." 15, no. 10: 1.
Water is a basic natural resource for life and the sustainable development of society. Methods to assess water quality in freshwater ecosystems based on environmental quality bioindicators have proven to be low cost, reliable, and can be adapted to ecosystems with well-defined structures. The objective of this paper is to propose an interdisciplinary approach for the assessment of water quality in freshwater ecosystems through bioindicators. From the presence/absence of bioindicator organisms and their sensitivity/tolerance to environmental stress, we constructed a bipartite network, G. In this direction, we propose a new method that combines two research approaches, Graph Theory and Random Matrix Theory (RMT). Through the topological properties of the graph G, we introduce a topological index, called J P ( G ) , to evaluate the water quality, and we study its properties and relationships with known indices, such as Biological Monitoring Working Party ( B M W P ) and Shannon diversity ( H ′ ). Furthermore, we perform a scaling analysis of random bipartite networks with already specialized parameters for our case study. We validate our proposal for its application in the reservoir of Guájaro, Colombia. The results obtained allow us to infer that the proposed techniques are useful for the study of water quality, since they detect significant changes in the ecosystem.
Jair Pineda-Pineda; Claudia-Teresa Martínez-Martínez; J. Méndez-Bermúdez; Jesús Muñoz-Rojas; José Sigarreta. Application of Bipartite Networks to the Study of Water Quality. Sustainability 2020, 12, 5143 .
AMA StyleJair Pineda-Pineda, Claudia-Teresa Martínez-Martínez, J. Méndez-Bermúdez, Jesús Muñoz-Rojas, José Sigarreta. Application of Bipartite Networks to the Study of Water Quality. Sustainability. 2020; 12 (12):5143.
Chicago/Turabian StyleJair Pineda-Pineda; Claudia-Teresa Martínez-Martínez; J. Méndez-Bermúdez; Jesús Muñoz-Rojas; José Sigarreta. 2020. "Application of Bipartite Networks to the Study of Water Quality." Sustainability 12, no. 12: 5143.
Catherine Cesa-Luna; Alberto Aguayo-Acosta; Antonino Baez; Jesús Muñoz-Rojas; Verónica Quintero-Hernández. Cold-leaching extraction. A new methodology for obtaining inhibitory substances produced by bacteria in solid media. v4. protocols.io 2020, 1 .
AMA StyleCatherine Cesa-Luna, Alberto Aguayo-Acosta, Antonino Baez, Jesús Muñoz-Rojas, Verónica Quintero-Hernández. Cold-leaching extraction. A new methodology for obtaining inhibitory substances produced by bacteria in solid media. v4. protocols.io. 2020; ():1.
Chicago/Turabian StyleCatherine Cesa-Luna; Alberto Aguayo-Acosta; Antonino Baez; Jesús Muñoz-Rojas; Verónica Quintero-Hernández. 2020. "Cold-leaching extraction. A new methodology for obtaining inhibitory substances produced by bacteria in solid media. v4." protocols.io , no. : 1.
Volatile organic compounds (VOCs) produced by rhizobacteria have been proven to stimulate plant growth during germination and seedling stages. However, the modulating effect of bacterial volatiles on the germination of seeds subjected to heavy metal stress is scarcely studied. In this work, the ability of volatiles released by Bacillus sp. MH778713 to induce seed dormancy breakage in Prosopis laevigata and Arabidopsis thaliana seeds were examined. The minimal inhibitory concentration of chromium (Cr) VI that prevents seed germination of P. laevigata and A. thaliana on water-Cr-agar plates was 2500 and 100 mg L–1, respectively. Remarkably, partitioned Petri-dish co-cultivation of Bacillus sp. MH778713 and plant seeds under Cr-stress showed the beneficial effect of volatiles emitted by Bacillus sp. MH778713, helping plant seeds to overcome Cr-stress. Among the metabolites emitted by Bacillus sp. MH778713, octadecane, heneicosane, 2,4-di-tert-butylphenol, hexadecane, eicosane, octacosane, and tetratriacontane were the most abundant. To confirm that these long-chain compounds produced by Bacillus sp. MH778713 could be responsible for the seed dormancy breakage, high pure organic compounds (2,4-di-tert-butylphenol, heneicosane, hentriacontane, and tetracosane) were used directly in germination assays of P. laevigata and A. thaliana seeds instead of volatiles emitted by Bacillus sp. MH778713. All organic compounds allowed Prosopis and Arabidopsis seeds to overcome Cr-toxicity and germinate. The results of this study provide new insight into the role of long-chain bacterial compounds produced by Bacillus sp. MH778713 as triggers of seed abiotic stress tolerance, surmounting chromium stress and stimulating seedling development.
Verónica Ramírez; José-Antonio Munive; Luis Cortes; Jesús Muñoz-Rojas; Roberto Portillo; Antonino Baez. Long-Chain Hydrocarbons (C21, C24, and C31) Released by Bacillus sp. MH778713 Break Dormancy of Mesquite Seeds Subjected to Chromium Stress. Frontiers in Microbiology 2020, 11, 741 .
AMA StyleVerónica Ramírez, José-Antonio Munive, Luis Cortes, Jesús Muñoz-Rojas, Roberto Portillo, Antonino Baez. Long-Chain Hydrocarbons (C21, C24, and C31) Released by Bacillus sp. MH778713 Break Dormancy of Mesquite Seeds Subjected to Chromium Stress. Frontiers in Microbiology. 2020; 11 ():741.
Chicago/Turabian StyleVerónica Ramírez; José-Antonio Munive; Luis Cortes; Jesús Muñoz-Rojas; Roberto Portillo; Antonino Baez. 2020. "Long-Chain Hydrocarbons (C21, C24, and C31) Released by Bacillus sp. MH778713 Break Dormancy of Mesquite Seeds Subjected to Chromium Stress." Frontiers in Microbiology 11, no. : 741.
Bacteria produce antimicrobial compounds to compete for nutrients and space in a particular habitat. Antagonistic interactions can be evaluated by several methodologies including the double-layer agar and simultaneous inhibition assays. Among the well-known inhibitory substances produced by bacteria are the broad-spectrum antibiotics, organic acids, siderophores, antifungal, and bacteriocins. The most studied bacterial genera able to produce these inhibitory substances are Enterococcus, Lactococcus, Streptomyces, Bacillus, Pseudomonas, Klebsiella, Escherichia, and Burkholderia. Some beneficial bacteria can promote plant growth and degrade toxic compounds in the environment representing an attractive solution to diverse issues in agriculture and soil pollution, particularly in fields with damaged soils where pesticides and fertilizers have been indiscriminately used. Beneficial bacteria may increase plant health by inhibiting pathogenic microorganisms; some examples include Gluconacetobacter diazotrophicus, Azospirullum brasilense, Pseudomonas fluorescens, Pseudomonas protegens, and Burkholderia tropica. However, most studies showing the antagonistic potential of these bacteria have been performed in vitro, and just a few of them have been evaluated in association with plants. Several inhibitory substances involved in pathogen antagonism have not been elucidated yet; in fact, we know only 1 % of the bacterial diversity in a natural environment leading us to assume that many other inhibitory substances remain unexplored. In this review, we will describe the characteristics of some antimicrobial compounds produced by beneficial bacteria, the principal methodologies performed to evaluate their production, modes of action, and their importance for biotechnological purposes.
Catherine Cesa-Luna; Antonino Baez; Verónica Quintero-Hernández; Joel De La Cruz-Enríquez; Ma Dolores Castañeda-Antonio; Jesús Muñoz-Rojas. The importance of antimicrobial compounds produced by beneficial bacteria on the biocontrol of phytopathogens. Acta Biológica Colombiana 2020, 25, 140 -154.
AMA StyleCatherine Cesa-Luna, Antonino Baez, Verónica Quintero-Hernández, Joel De La Cruz-Enríquez, Ma Dolores Castañeda-Antonio, Jesús Muñoz-Rojas. The importance of antimicrobial compounds produced by beneficial bacteria on the biocontrol of phytopathogens. Acta Biológica Colombiana. 2020; 25 (1):140-154.
Chicago/Turabian StyleCatherine Cesa-Luna; Antonino Baez; Verónica Quintero-Hernández; Joel De La Cruz-Enríquez; Ma Dolores Castañeda-Antonio; Jesús Muñoz-Rojas. 2020. "The importance of antimicrobial compounds produced by beneficial bacteria on the biocontrol of phytopathogens." Acta Biológica Colombiana 25, no. 1: 140-154.
Scorpion venom peptides represent a novel source of antimicrobial peptides (AMPs) with broad-spectrum activity. In this study, we determined the minimum bactericidal concentration (MBC) of three scorpion AMPs, Uy234, Uy17, and Uy192, which are found in the venomous glands of the Urodacus yaschenkoi scorpion, against the clinical isolates of multidrug-resistant (MDR) bacteria. In addition, we tested the activity of a consensus AMP designed in our laboratory based on some previously reported IsCT-type (cytotoxic linear peptide) AMPs with the aim of obtaining higher antimicrobial activity. All peptides tested showed high antimicrobial activity against MDR clinical isolates, with the highest activity against β-hemolytic Streptococcus strains. The hemolytic activity was determined against human red blood cells and was significantly lower than that of previously reported AMPs. The α-helical structure of the four AMPs was confirmed by circular dichroism (CD). These results suggest that the four peptides can be valuable tools for the design and development of AMPs for use in the inhibition of MDR pathogenic bacteria. A clear index of synergism and additivity was found for the combination of QnCs-BUAP + Uy234, which makes these peptides the most promising candidates against pathogenic bacteria.
Catherine Cesa-Luna; Jesús Muñoz-Rojas; Gloria Saab-Rincon; Antonino Baez; Yolanda Elizabeth Morales-García; Víctor Rivelino Juárez-González; Verónica Quintero-Hernández. Structural characterization of scorpion peptides and their bactericidal activity against clinical isolates of multidrug-resistant bacteria. PLOS ONE 2019, 14, e0222438 .
AMA StyleCatherine Cesa-Luna, Jesús Muñoz-Rojas, Gloria Saab-Rincon, Antonino Baez, Yolanda Elizabeth Morales-García, Víctor Rivelino Juárez-González, Verónica Quintero-Hernández. Structural characterization of scorpion peptides and their bactericidal activity against clinical isolates of multidrug-resistant bacteria. PLOS ONE. 2019; 14 (11):e0222438.
Chicago/Turabian StyleCatherine Cesa-Luna; Jesús Muñoz-Rojas; Gloria Saab-Rincon; Antonino Baez; Yolanda Elizabeth Morales-García; Víctor Rivelino Juárez-González; Verónica Quintero-Hernández. 2019. "Structural characterization of scorpion peptides and their bactericidal activity against clinical isolates of multidrug-resistant bacteria." PLOS ONE 14, no. 11: e0222438.
In northern Mexico, aridity, salinity and high temperatures limit areas that can be cultivated. To investigate the nature of nitrogen-fixing symbionts of Phaseolus filiformis, an adapted wild bean species native to this region, their phylogenies were inferred by MLSA. Most rhizobia recovered belong to the proposed new species Ensifer aridi. Phylogenetic analyses of nodC and nifH show that Mexican isolates carry symbiotic genes acquired through horizontal gene transfer that are divergent from those previously characterized among bean symbionts. These strains are salt tolerant, able to grow in alkaline conditions, high temperatures, and capable of utilizing a wide range of carbohydrates and organic acids as carbon sources for growth. This study improves the knowledge on diversity, geographic distribution and evolution of bean-nodulating rhizobia in Mexico and further enlarges the spectrum of microsymbiont with which Phaseolus species can interact with, including cultivated bean varieties, notably under stressed environments. Here, the species Ensifer aridi sp. nov. is proposed as strain type of the Moroccan isolate LMR001T (= LMG 31426T; = HAMBI 3707T) recovered from desert sand dune.
Guadalupe Rocha; Antoine Le Queré; Arturo Medina; Alma Cuéllar; José-Luis Contreras; Ricardo Carreño-López; Rocío Bustillos; Jesús Muñoz-Rojas; María Del Carmen Villegas; Clémence Chaintreuil; Bernard Dreyfus; José-Antonio Munive. Diversity and phenotypic analyses of salt- and heat-tolerant wild bean Phaseolus filiformis rhizobia native of a sand beach in Baja California and description of Ensifer aridi sp. nov. Archives of Microbiology 2019, 202, 309 -322.
AMA StyleGuadalupe Rocha, Antoine Le Queré, Arturo Medina, Alma Cuéllar, José-Luis Contreras, Ricardo Carreño-López, Rocío Bustillos, Jesús Muñoz-Rojas, María Del Carmen Villegas, Clémence Chaintreuil, Bernard Dreyfus, José-Antonio Munive. Diversity and phenotypic analyses of salt- and heat-tolerant wild bean Phaseolus filiformis rhizobia native of a sand beach in Baja California and description of Ensifer aridi sp. nov. Archives of Microbiology. 2019; 202 (2):309-322.
Chicago/Turabian StyleGuadalupe Rocha; Antoine Le Queré; Arturo Medina; Alma Cuéllar; José-Luis Contreras; Ricardo Carreño-López; Rocío Bustillos; Jesús Muñoz-Rojas; María Del Carmen Villegas; Clémence Chaintreuil; Bernard Dreyfus; José-Antonio Munive. 2019. "Diversity and phenotypic analyses of salt- and heat-tolerant wild bean Phaseolus filiformis rhizobia native of a sand beach in Baja California and description of Ensifer aridi sp. nov." Archives of Microbiology 202, no. 2: 309-322.
Plant growth-promoting bacteria (PGPB) have been extensively studied, because of different mechanisms to perform phytostimulation, as well as the abilities to colonize plants. The number of crop types and hectares of agricultural land, where PGPR are applied is low compared with the total crops or farming area. However, the PGPB application in crop fields is increasingly becoming more accepted due to the advantages to crops and environment such as the increment in productivity, the diminution in the use of chemical fertilizers and toxic compounds such as pesticides and herbicides. These traits make beneficial bacteria formulations the ‘right choice’ in healthy agriculture since they are highly compatible with sustainable crop production. The co-inoculation of plants with two or more beneficial bacteria apparently provides greater phytostimulation than mono-inoculation, perhaps because of the synergistic and multifarious effects occurring when two or more microorganisms are co-interacting. There is a consensus that bacterial mixtures provide greater benefits to the plants, the number of formulations containing more than three species of microorganisms in consortium is still limited. Therefore, we believe that more research and investment is needed to design and formulate multi-species inoculants containing compatible bacteria and other beneficial microorganisms in order to be capable of coexisting both in the designed formulation and associated with plants for sustainable benefits.
Yolanda Elizabeth Morales-García; Antonino Baez; Verónica Quintero-Hernández; Dalia Molina-Romero; América Paulina Rivera-Urbalejo; Laura Abisaí Pazos-Rojas; Jesús Muñoz-Rojas. Bacterial Mixtures, the Future Generation of Inoculants for Sustainable Crop Production. Sustainable Development and Biodiversity 2019, 11 -44.
AMA StyleYolanda Elizabeth Morales-García, Antonino Baez, Verónica Quintero-Hernández, Dalia Molina-Romero, América Paulina Rivera-Urbalejo, Laura Abisaí Pazos-Rojas, Jesús Muñoz-Rojas. Bacterial Mixtures, the Future Generation of Inoculants for Sustainable Crop Production. Sustainable Development and Biodiversity. 2019; ():11-44.
Chicago/Turabian StyleYolanda Elizabeth Morales-García; Antonino Baez; Verónica Quintero-Hernández; Dalia Molina-Romero; América Paulina Rivera-Urbalejo; Laura Abisaí Pazos-Rojas; Jesús Muñoz-Rojas. 2019. "Bacterial Mixtures, the Future Generation of Inoculants for Sustainable Crop Production." Sustainable Development and Biodiversity , no. : 11-44.
Introduction:Several plant-beneficial bacteria have the capability to promote the growth of plants through different mechanisms. The survival of such bacteria could be affected by environmental abiotic factors compromising their capabilities of phytostimulation. One of the limiting abiotic factors is low water availability.Materials and Methods:In extreme cases, bacterial cells can suffer desiccation, which triggers harmful effects on cells. Bacteria tolerant to desiccation have developed different strategies to cope with these conditions; however, the genes involved in these processes have not been sufficiently explored.Klebsiella variicolaT29A is a beneficial bacterial strain that promotes the growth of corn plants and is highly tolerant to desiccation. In the present work, we investigated genes involved in desiccation tolerance.Results & Discussion:As a result, a library of 8974 mutants of this bacterial strain was generated by random mutagenesis with mini-Tn5 transposon, and mutants that lost the capability to tolerate desiccation were selected. We found 14 sensitive mutants; those with the lowest bacterial survival rate contained mini-Tn5 transposon inserted into genes encoding a protein domain related to BetR, putative secretion ATPase and dihydroorotase. The mutant in the betR gene had the lowest survival; therefore, the mutagenized gene was validated using specific amplification and sequencing.Conclusion:Trans complementation with the wild-type gene improved the survival of the mutant under desiccation conditions, showing that this gene is a determinant for the survival ofK. variicolaT29A under desiccation conditions.
Osvaldo Rodríguez-Andrade; Andrés Corral-Lugo; Yolanda Elizabeth Morales-García; Verónica Quintero-Hernández; América P. Rivera-Urbalejo; Dalia Molina-Romero; Rebeca D. Martínez-Contreras; Patricia Bernal; Jesús Muñoz-Rojas. Identification of Klebsiella Variicola T29A Genes Involved In Tolerance To Desiccation. The Open Microbiology Journal 2019, 13, 256 -267.
AMA StyleOsvaldo Rodríguez-Andrade, Andrés Corral-Lugo, Yolanda Elizabeth Morales-García, Verónica Quintero-Hernández, América P. Rivera-Urbalejo, Dalia Molina-Romero, Rebeca D. Martínez-Contreras, Patricia Bernal, Jesús Muñoz-Rojas. Identification of Klebsiella Variicola T29A Genes Involved In Tolerance To Desiccation. The Open Microbiology Journal. 2019; 13 (1):256-267.
Chicago/Turabian StyleOsvaldo Rodríguez-Andrade; Andrés Corral-Lugo; Yolanda Elizabeth Morales-García; Verónica Quintero-Hernández; América P. Rivera-Urbalejo; Dalia Molina-Romero; Rebeca D. Martínez-Contreras; Patricia Bernal; Jesús Muñoz-Rojas. 2019. "Identification of Klebsiella Variicola T29A Genes Involved In Tolerance To Desiccation." The Open Microbiology Journal 13, no. 1: 256-267.
Heavy metal accumulation in mesquite trees (Prosopis laevigata) growing in aluminum, titanium, chromium and zirconium-polluted soils of a semi-arid region in Mexico was investigated using wavelength dispersive X-ray fluorescence analysis. The results showed that P. laevigata trees can hyper accumulate up to 4100 mg/kg of Al, 14000 mg/kg of Fe, 1600 mg/kg of Ti, 2500 mg/kg of Zn, but not chromium, regarding high chromium concentrations found in soils (435 mg/kg). Since plant-associated microorganism can modulate phytoremediation efficiency, the biodiversity of P. laevigata associated bacteria was studied. Eighty-eight isolates from P. laevigata nodules were obtained; all isolates tolerated high concentrations of Al, Fe, Zn and Cr in vitro. The top-six chromium tolerant strains were identified by 16S rRNA sequence analysis as belonging to genus Bacillus. Bacillus sp. MH778713, close to Bacillus cereus group, showed to be the most resistant strain, tolerating up to 15000 mg/L Cr (VI) and 10000 mg/L of Al. Regarding the bioaccumulation traits, Bacillus sp. MH778713 accumulated up to 100 mg Cr(VI)/g of cells when it was exposed to 1474 mg/L of Cr VI. To assess Bacillus sp. MH778713 ability to assist Prosopis laevigata phytoremediation; twenty plants were inoculated or non-inoculated with Bacillus sp. MH778713 and grown in nitrogen-free Jensen’s medium added with 0, 10 and 25 mg/L of Cr(VI). Only plants inoculated with Bacillus sp. grew in the presence of chromium showing the ability of this strain to assist chromium phytoremediation. P. laevigata and Bacillus spp. may be considered as good candidates for soil restoration of arid and semiarid sites contaminated with heavy metals.
Verónica Ramírez; Antonino Baez; Primavera López; Rocío Bustillos; Miguel Ángel Villalobos; Ricardo Carreño; José Luis Contreras; Jesús Muñoz-Rojas; Luis Ernesto Fuentes; Javier Martínez; José Antonio Munive. Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata). Frontiers in Microbiology 2019, 10, 1833 .
AMA StyleVerónica Ramírez, Antonino Baez, Primavera López, Rocío Bustillos, Miguel Ángel Villalobos, Ricardo Carreño, José Luis Contreras, Jesús Muñoz-Rojas, Luis Ernesto Fuentes, Javier Martínez, José Antonio Munive. Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata). Frontiers in Microbiology. 2019; 10 ():1833.
Chicago/Turabian StyleVerónica Ramírez; Antonino Baez; Primavera López; Rocío Bustillos; Miguel Ángel Villalobos; Ricardo Carreño; José Luis Contreras; Jesús Muñoz-Rojas; Luis Ernesto Fuentes; Javier Martínez; José Antonio Munive. 2019. "Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata)." Frontiers in Microbiology 10, no. : 1833.
The potential of Pseudomonas putida KT2440 to act as a plant-growth promoter or as a bioremediator of toxic compounds can be affected by desiccation. In the present work, the bacterial survival ratio (BSR) in response to air desiccation was evaluated for P. putida KT2440 in the presence of different protectors. The BSR in the presence of nonreducing disaccharides, such as trehalose, was high after 15 days of desiccation stress (occurring at 30°C and 50% relative humidity), whereas in the absence of a protector the bacterial counts diminished to nondetectable numbers (ca 2.8 log CFU/mL). The LIVE/DEAD staining method showed that bacteria protected with trehalose maintained increased numbers of green cells after desiccation while cells without protection were all observed to be red. This indicated that nonprotected bacteria had compromised membrane integrity. However, when nonprotected bacteria subjected to 18 days of desiccation stress were rehydrated for a short time with maize root exudates or for 48 h with water (prolonged rehydration), the bacterial counts were as high as that observed for those not subjected to desiccation stress, suggesting that the cells entered the viable but nonculturable (VBNC) state under desiccation and that they returned to a culturable state after those means of rehydration. Interestingly an increase in the green color intensity of cells that returned to a culturable state was observed using LIVE/DEAD staining method, indicating an improvement in their membrane integrity. Cellular activity in the VBNC state was determined. A GFP-tagged P. putida strain expressing GFP constitutively was subjected to desiccation. After 12 days of desiccation, the GFP-tagged strain lost culturability, but it exhibited active GFP expression, which in turn made the cells green. Furthermore, the expression of 16S rRNA, rpoN (housekeeping), mutL, mutS (encoding proteins from the mismatch repair complex), and oprH (encoding an outer membrane protein) were examined by RT-PCR. All evaluated genes were expressed by both types of cells, culturable and nonculturable, indicating active molecular processes during the VBNC state.
Laura Abisaí Pazos-Rojas; Ligia Catalina Muñoz-Arenas; Osvaldo Rodríguez-Andrade; Lesther Emanuel López-Cruz; Orestes López-Ortega; Fábio Lopes-Olivares; Silvia Luna-Suarez; Antonino Baez; Yolanda Elizabeth Morales-García; Verónica Quintero-Hernández; Miguel Angel Villalobos-López; Jesús De la Torre; Jesús Muñoz-Rojas. Desiccation-induced viable but nonculturable state in Pseudomonas putida KT2440, a survival strategy. PLOS ONE 2019, 14, e0219554 .
AMA StyleLaura Abisaí Pazos-Rojas, Ligia Catalina Muñoz-Arenas, Osvaldo Rodríguez-Andrade, Lesther Emanuel López-Cruz, Orestes López-Ortega, Fábio Lopes-Olivares, Silvia Luna-Suarez, Antonino Baez, Yolanda Elizabeth Morales-García, Verónica Quintero-Hernández, Miguel Angel Villalobos-López, Jesús De la Torre, Jesús Muñoz-Rojas. Desiccation-induced viable but nonculturable state in Pseudomonas putida KT2440, a survival strategy. PLOS ONE. 2019; 14 (7):e0219554.
Chicago/Turabian StyleLaura Abisaí Pazos-Rojas; Ligia Catalina Muñoz-Arenas; Osvaldo Rodríguez-Andrade; Lesther Emanuel López-Cruz; Orestes López-Ortega; Fábio Lopes-Olivares; Silvia Luna-Suarez; Antonino Baez; Yolanda Elizabeth Morales-García; Verónica Quintero-Hernández; Miguel Angel Villalobos-López; Jesús De la Torre; Jesús Muñoz-Rojas. 2019. "Desiccation-induced viable but nonculturable state in Pseudomonas putida KT2440, a survival strategy." PLOS ONE 14, no. 7: e0219554.
A random transposition mutant library of B. gladioli UAPS07070 was analyzed for searching mutants with impaired microbial antagonism. Three derivates showed diminished antimicrobial activity against a sensitive strain. The mutated loci showed high similarity to the quorum sensing genes of the AHL-synthase and its regulator. Another mutant was affected in a gene coding for a LysrR-type transcriptional regulator. The production of toxoflavin, the most well known antimicrobial-molecule and a major virulence factor of plant-pathogenic B. glumae and B. gladioli was explored. The absence of a yellowish pigment related to toxoflavin and the undetectable transcription of toxA in the mutants indicated the participation of the QS system and of the LysR-type transcriptional regulator in the regulation of toxoflavin. Additionally, those genes were found to be related to the swarming phenotype. Lettuce inoculated with the AHL synthase and the lysR mutants showed less severe symptoms. We present evidence of the participation of both, the quorum sensing and for the first time, of a LysR-type transcriptional regulator in antibiosis and swarming phenotype in a strain of B. gladioli
Eduardo Seynos; M. Castañeda-Lucio; Jesús Muñoz-Rojas; L. López-Pliego; Miguel Angel Villalobos-López; Rocío Bustillos; L. E. Fuentes-Ramírez. Loci identification of a N-acyl homoserine lactone type quorum sensing system and a new LysR-type transcriptional regulator associated with antimicrobial activity and swarming in Burkholderia gladioli UAPS07070. Open Life Sciences 2019, 14, 165 -178.
AMA StyleEduardo Seynos, M. Castañeda-Lucio, Jesús Muñoz-Rojas, L. López-Pliego, Miguel Angel Villalobos-López, Rocío Bustillos, L. E. Fuentes-Ramírez. Loci identification of a N-acyl homoserine lactone type quorum sensing system and a new LysR-type transcriptional regulator associated with antimicrobial activity and swarming in Burkholderia gladioli UAPS07070. Open Life Sciences. 2019; 14 (1):165-178.
Chicago/Turabian StyleEduardo Seynos; M. Castañeda-Lucio; Jesús Muñoz-Rojas; L. López-Pliego; Miguel Angel Villalobos-López; Rocío Bustillos; L. E. Fuentes-Ramírez. 2019. "Loci identification of a N-acyl homoserine lactone type quorum sensing system and a new LysR-type transcriptional regulator associated with antimicrobial activity and swarming in Burkholderia gladioli UAPS07070." Open Life Sciences 14, no. 1: 165-178.
El residuo de la cáscara de naranja es un contaminante común en México, a pesar de que puede usarse en las áreas cosmética, farmacéutica y alimenticia. Extrajimos el aceite de naranja a partir de cáscara residual y determinamos sus compuestos químicos y su actividad antimicrobiana. Utilizamos la destilación Soxhlet, extracción líquido-líquido y extracción de prensado, y cromatografía de gases acoplada a espectrometría de masas (GC-MS). Usamos una técnica de Kirby & Bauer modificada en Acremonium sp., Alternaria sp., Aspergillus niger, Candida albicans, Pseudomonas aeruginosa ATCC27853, Staphylococcus aureus ATCC 25923, Trichoderma sp. y Verticillium sp. El método Soxhlet fue el más eficiente (2,065%). Observamos valores de inhibición de 75% a 100% para S. aureus y P. auroginosa; 75 a 100% para Candida albicans; y 50 a 74% para Pseudomonas auroginosa. Actuó como fungistático para todas las cepas. El perfil CG-MS fue: D-limoneno 85,93%, β-mirceno 2,23%, linalool 1,72%, decanal 0,63%, α-pineno 0,43% y sabineno 0,20% por μL de aceite por espacio de cabeza (Head-space) y por inyección directa (principales componentes: alcanos y alcohol perílico, D-limoneno y linalool).
Dolores Castañeda-Antonio; Antonio Rivera-Tapia; Esther Choy Flores; Ricardo Munguía-Pérez; Roberto Portillo-Reyes; Jesús Muñoz. Actividad antimicrobiana del aceite de naranja residual. UNED Research Journal 2018, 10, 469 -474.
AMA StyleDolores Castañeda-Antonio, Antonio Rivera-Tapia, Esther Choy Flores, Ricardo Munguía-Pérez, Roberto Portillo-Reyes, Jesús Muñoz. Actividad antimicrobiana del aceite de naranja residual. UNED Research Journal. 2018; 10 (2):469-474.
Chicago/Turabian StyleDolores Castañeda-Antonio; Antonio Rivera-Tapia; Esther Choy Flores; Ricardo Munguía-Pérez; Roberto Portillo-Reyes; Jesús Muñoz. 2018. "Actividad antimicrobiana del aceite de naranja residual." UNED Research Journal 10, no. 2: 469-474.
Se realizó el aislamiento y selección según su mecanismo de acción de bacterias endofíticas y rizosféricas promotoras de crecimiento vegetal de poblaciones silvestres de Pinus patula y Pinus montezumae, grupos arbóreos de coníferas mexicanas utilizados con fines de reforestación por su crecimiento acelerado y alto rendimiento. Ochenta y siete cepas bacterianas fueron aisladas de plántulas de pino, purificadas y agrupadas por criterios morfológicos. Se evaluaron y seleccionaron cepas que producen fitoreguladores de crecimiento, solubilización de fosfato, determinación de la cantidad de fósforo solubilizado y producción de quitinasa. Treinta y siete cepas mostraron al menos una de las actividades evaluadas, con niveles de producción de fitorreguadores en parámetros que van de 54.4 a 139 µg/ml, halos alrededor de las colonias de 3.2 a 3.8 cm solubilizando fosfato en cantidades que varían de 0.1 a 2.4 mg/L y crecimiento completo con zonas claras alrededor de las colonias con halos de 1.2 a 2 cm para la producción de quitinasa. Los resultados de este trabajo representan el primer paso para desarrollar un sistema de aceleración de crecimiento para plantas de uso forestal.
F. David Moreno-Valencia; Miguel Ángel Plascencia-Espinosa; Jesús Muñoz-Rojas; Instituto Politécnico Nacional. Ex-Hacienda San Juan Molino Centro De Investigación En Biotecnología Aplicada (Ciba); Instituto De Ciencias Centro De Investigaciones En Ciencias Microbiológicas (Cicm). Aislamiento y selección de bacterias promotoras de crecimiento vegetal para su aplicación en especies forestales. Mexican journal of biotechnology 2018, 3, 36 -53.
AMA StyleF. David Moreno-Valencia, Miguel Ángel Plascencia-Espinosa, Jesús Muñoz-Rojas, Instituto Politécnico Nacional. Ex-Hacienda San Juan Molino Centro De Investigación En Biotecnología Aplicada (Ciba), Instituto De Ciencias Centro De Investigaciones En Ciencias Microbiológicas (Cicm). Aislamiento y selección de bacterias promotoras de crecimiento vegetal para su aplicación en especies forestales. Mexican journal of biotechnology. 2018; 3 (3):36-53.
Chicago/Turabian StyleF. David Moreno-Valencia; Miguel Ángel Plascencia-Espinosa; Jesús Muñoz-Rojas; Instituto Politécnico Nacional. Ex-Hacienda San Juan Molino Centro De Investigación En Biotecnología Aplicada (Ciba); Instituto De Ciencias Centro De Investigaciones En Ciencias Microbiológicas (Cicm). 2018. "Aislamiento y selección de bacterias promotoras de crecimiento vegetal para su aplicación en especies forestales." Mexican journal of biotechnology 3, no. 3: 36-53.
Plant growth-promoting rhizobacteria (PGPR) increase plant growth and crop productivity. The inoculation of plants with a bacterial mixture (consortium) apparently provides greater benefits to plant growth than inoculation with a single bacterial strain. In the present work, a bacterial consortium was formulated containing four compatible and desiccation-tolerant strains with potential as PGPR. The formulation had one moderately (Pseudomonas putida KT2440) and three highly desiccation-tolerant (Sphingomonas sp. OF178, Azospirillum brasilense Sp7 and Acinetobacter sp. EMM02) strains. The four bacterial strains were able to adhere to seeds and colonize the rhizosphere of plants when applied in both mono-inoculation and multi-inoculation treatments, showing that they can also coexist without antagonistic effects in association with plants. The effects of the bacterial consortium on the growth of blue maize were evaluated. Seeds inoculated with either individual bacterial strains or the bacterial consortium were subjected to two experimental conditions before sowing: normal hydration or desiccation. In general, inoculation with the bacterial consortium increased the shoot and root dry weight, plant height and plant diameter compared to the non-inoculated control or mono-inoculation treatments. The bacterial consortium formulated in this work had greater benefits for blue maize plants even when the inoculated seeds underwent desiccation stress before germination, making this formulation attractive for future field applications.
Dalia Molina-Romero; Antonino Baez; Verónica Quintero-Hernández; Miguel Castañeda-Lucio; Luis Fuentes; Rocío Bustillos; Osvaldo Rodríguez-Andrade; Yolanda Elizabeth Morales-García; José-Antonio Munive; Jesús Muñoz-Rojas. Compatible bacterial mixture, tolerant to desiccation, improves maize plant growth. PLOS ONE 2017, 12, e0187913 .
AMA StyleDalia Molina-Romero, Antonino Baez, Verónica Quintero-Hernández, Miguel Castañeda-Lucio, Luis Fuentes, Rocío Bustillos, Osvaldo Rodríguez-Andrade, Yolanda Elizabeth Morales-García, José-Antonio Munive, Jesús Muñoz-Rojas. Compatible bacterial mixture, tolerant to desiccation, improves maize plant growth. PLOS ONE. 2017; 12 (11):e0187913.
Chicago/Turabian StyleDalia Molina-Romero; Antonino Baez; Verónica Quintero-Hernández; Miguel Castañeda-Lucio; Luis Fuentes; Rocío Bustillos; Osvaldo Rodríguez-Andrade; Yolanda Elizabeth Morales-García; José-Antonio Munive; Jesús Muñoz-Rojas. 2017. "Compatible bacterial mixture, tolerant to desiccation, improves maize plant growth." PLOS ONE 12, no. 11: e0187913.
The manuscript published by Microb Biotechnol, volume 10, highlights the relevance of the fungus Nigrospora sphaerica, an endophyte isolated from Catharanthus roseus, as an alternative source to obtain vinblastine, a compound used in chemotherapy schemes to treat several types of cancer. Authors showed that purification of vinblastine from extracts of the fungus has higher activity and yield in comparison with that obtained from the plant Catharanthus roseus. This work represents a biotechnological approach to obtain vinblastine with promising results to decrease the production cost.
Jesús Muñoz‐Rojas. Importance of producing economic compounds to combat cancer. Microbial Biotechnology 2017, 10, 683 -684.
AMA StyleJesús Muñoz‐Rojas. Importance of producing economic compounds to combat cancer. Microbial Biotechnology. 2017; 10 (4):683-684.
Chicago/Turabian StyleJesús Muñoz‐Rojas. 2017. "Importance of producing economic compounds to combat cancer." Microbial Biotechnology 10, no. 4: 683-684.
Methanol-consuming culturable bacteria were isolated from the plant surface, rhizosphere, and inside the stem of Neobuxbaumia macrocephala. All 38 isolates were facultative methylotrophic microorganisms. Their classification included the Classes Actinobacteria, Sphingobacteriia, Alpha-, Beta-, and Gammaproteobacteria. The deduced amino acid sequences of methanol dehydrogenase obtained by PCR belonging to Actinobacteria, Alpha-, Beta-, and Gammaproteobacteria showed high similarity to rare-earth element (REE)-dependent XoxF methanol dehydrogenases, particularly the group XoxF5. The sequences included Asp301, the REE-coordinating amino acid, present in all known XoxF dehydrogenases and absent in MxaF methanol dehydrogenases. The quantity of the isolates showed positive hybridization with a xoxF probe, but not with a mxaF probe. Isolates of all taxonomic groups showed methylotrophic growth in the presence of Ce3+ or Ca2+. The presence of xoxF-like sequences in methylotrophic bacteria from N. macrocephala and its potential relationship with their adaptability to xerophytic plants are discussed.
Rocío Bustillos; Ivan Corona-Gutierrez; Miguel Castañeda-Lucio; Carolina Águila-Zempoaltécatl; Eduardo Seynos; Ismael Hernández-Lucas; Jesús Muñoz-Rojas; Liliana Medina-Aparicio; Luis Ernesto Fuentes-Ramírez. Culturable Facultative Methylotrophic Bacteria from the Cactus Neobuxbaumia macrocephala Possess the Locus xoxF and Consume Methanol in the Presence of Ce3+ and Ca2+. Microbes and Environments 2017, 32, 244 -251.
AMA StyleRocío Bustillos, Ivan Corona-Gutierrez, Miguel Castañeda-Lucio, Carolina Águila-Zempoaltécatl, Eduardo Seynos, Ismael Hernández-Lucas, Jesús Muñoz-Rojas, Liliana Medina-Aparicio, Luis Ernesto Fuentes-Ramírez. Culturable Facultative Methylotrophic Bacteria from the Cactus Neobuxbaumia macrocephala Possess the Locus xoxF and Consume Methanol in the Presence of Ce3+ and Ca2+. Microbes and Environments. 2017; 32 (3):244-251.
Chicago/Turabian StyleRocío Bustillos; Ivan Corona-Gutierrez; Miguel Castañeda-Lucio; Carolina Águila-Zempoaltécatl; Eduardo Seynos; Ismael Hernández-Lucas; Jesús Muñoz-Rojas; Liliana Medina-Aparicio; Luis Ernesto Fuentes-Ramírez. 2017. "Culturable Facultative Methylotrophic Bacteria from the Cactus Neobuxbaumia macrocephala Possess the Locus xoxF and Consume Methanol in the Presence of Ce3+ and Ca2+." Microbes and Environments 32, no. 3: 244-251.
In this Crystal Ball we describe the negative effects of the scheme of intensive agriculture of the green revolution technology. To recover the contaminated soils derived from intensive farming is necessary introduce new successful technologies to replace the use of chemical fertilizer and toxic pesticides by organic fertilizers and biological control agents. Our principal speculation is that in a short time authors in the field of PGPB and bioremediation will be expanding the knowledge on the development of different formulations containing super-bacteria or a mixture of super-bacteria able to provide beneficial effect for agriculture and bioremediation.
Antonino Baez; Yolanda Elizabeth Morales-García; Verónica Quintero-Hernández; Jesús Muñoz-Rojas. Next generation of microbial inoculants for agriculture and bioremediation. Microbial Biotechnology 2016, 10, 19 -21.
AMA StyleAntonino Baez, Yolanda Elizabeth Morales-García, Verónica Quintero-Hernández, Jesús Muñoz-Rojas. Next generation of microbial inoculants for agriculture and bioremediation. Microbial Biotechnology. 2016; 10 (1):19-21.
Chicago/Turabian StyleAntonino Baez; Yolanda Elizabeth Morales-García; Verónica Quintero-Hernández; Jesús Muñoz-Rojas. 2016. "Next generation of microbial inoculants for agriculture and bioremediation." Microbial Biotechnology 10, no. 1: 19-21.
It has been established that a decrease in the population of Gluconacetobacter diazotrophicus associated with sugarcane occurs after nitrogen fertilization. This fact could be due to a direct influence of NH4NO3 on bacterial cells or to changes in plant physiology after fertilizer addition, affecting bacterial establishment. In this work, we observed that survival of G. diazotrophicus was directly influenced when 44.8mM of NH4NO3 (640mgN/plant) was used for in vitro experiments. Furthermore, micropropagated sugarcane plantlets were inoculated with G. diazotrophicus and used for split root experiments, in which both ends of the system were fertilized with a basal level of NH4NO3 (0.35mM; 10mgN/plant). Twenty days post inoculation (dpi) one half of the plants were fertilized with a high dose of NH4NO3 (6.3mM; 180mgN/plant) on one end of the system. This nitrogen level was lower than that directly affecting G. diazotrophicus cells; however, it caused a decrease in the bacterial population in comparison with control plants fertilized with basal nitrogen levels. The decrease in the population of G. diazotrophicus was higher in pots fertilized with a basal nitrogen level when compared with the corresponding end supplied with high levels of NH4NO3 (100dpi; 80 days post fertilization) of the same plant system. These observations suggest that the high nitrogen level added to the plants induce systemic physiological changes that affect the establishment of G. diazotrophicus
Osvaldo Rodríguez-Andrade; Luis E. Fuentes-Ramírez; Yolanda E. Morales-García; Dalia Molina-Romero; María R. Bustillos-Cristales; Rebeca D. Martínez-Contreras; Jesús Muñoz-Rojas. The decrease in the population of Gluconacetobacter diazotrophicus in sugarcane after nitrogen fertilization is related to plant physiology in split root experiments. Revista Argentina de Microbiología 2015, 47, 335 -343.
AMA StyleOsvaldo Rodríguez-Andrade, Luis E. Fuentes-Ramírez, Yolanda E. Morales-García, Dalia Molina-Romero, María R. Bustillos-Cristales, Rebeca D. Martínez-Contreras, Jesús Muñoz-Rojas. The decrease in the population of Gluconacetobacter diazotrophicus in sugarcane after nitrogen fertilization is related to plant physiology in split root experiments. Revista Argentina de Microbiología. 2015; 47 (4):335-343.
Chicago/Turabian StyleOsvaldo Rodríguez-Andrade; Luis E. Fuentes-Ramírez; Yolanda E. Morales-García; Dalia Molina-Romero; María R. Bustillos-Cristales; Rebeca D. Martínez-Contreras; Jesús Muñoz-Rojas. 2015. "The decrease in the population of Gluconacetobacter diazotrophicus in sugarcane after nitrogen fertilization is related to plant physiology in split root experiments." Revista Argentina de Microbiología 47, no. 4: 335-343.
The PctC chemoreceptor of Pseudomonas aeruginosa mediates chemotaxis with high specificity to gamma‐aminobutyric acid (GABA). This compound is present everywhere in nature and has multiple functions, including being a human neurotransmitter or plant signaling compound. Because P. aeruginosa is ubiquitously distributed in nature and able to infect and colonize different hosts, the physiological relevance of GABA taxis is unclear, but it has been suggested that bacterial attraction to neurotransmitters may enhance virulence. We report the identification of McpG as a specific GABA chemoreceptor in non‐pathogenic Pseudomonas putida KT2440. As with PctC, GABA was found to bind McpG tightly. The analysis of chimeras comprising the PctC and McpG ligand‐binding domains fused to the Tar signaling domain showed very high GABA sensitivities. We also show that PctC inactivation does not alter virulence in Caenorhabditis elegans. Significant amounts of GABA were detected in tomato root exudates, and deletion of mcpG reduced root colonization that requires chemotaxis through agar. The C. elegans data and the detection of a GABA receptor in non‐pathogenic species indicate that GABA taxis may not be related to virulence in animal systems but may be of importance in the context of colonization and infection of plant roots by soil‐dwelling pseudomonads.
Jose Antonio Reyes-Darias; Vanina García; Miriam Rico-Jiménez; Andrés Corral-Lugo; Olivier Lesouhaitier; Dalia Juárez-Hernández; Yiling Yang; Shuangyu Bi; Marc Feuilloley; Jesús Muñoz-Rojas; Victor Sourjik; Tino Krell. Specific gamma-aminobutyrate chemotaxis in pseudomonads with different lifestyle. Molecular Microbiology 2015, 97, 488 -501.
AMA StyleJose Antonio Reyes-Darias, Vanina García, Miriam Rico-Jiménez, Andrés Corral-Lugo, Olivier Lesouhaitier, Dalia Juárez-Hernández, Yiling Yang, Shuangyu Bi, Marc Feuilloley, Jesús Muñoz-Rojas, Victor Sourjik, Tino Krell. Specific gamma-aminobutyrate chemotaxis in pseudomonads with different lifestyle. Molecular Microbiology. 2015; 97 (3):488-501.
Chicago/Turabian StyleJose Antonio Reyes-Darias; Vanina García; Miriam Rico-Jiménez; Andrés Corral-Lugo; Olivier Lesouhaitier; Dalia Juárez-Hernández; Yiling Yang; Shuangyu Bi; Marc Feuilloley; Jesús Muñoz-Rojas; Victor Sourjik; Tino Krell. 2015. "Specific gamma-aminobutyrate chemotaxis in pseudomonads with different lifestyle." Molecular Microbiology 97, no. 3: 488-501.