A 60-day trial was conducted in rainbow trout (Oncorhynchus mykiss) fry (initial weight = 2.5 ± 0.6 g) to evaluate the potential use of a bacterial single-cell protein (SCP) as an alternative protein source. Five experimental diets with different levels of fishmeal replacement (0, 25, 50, 75 and 100%) and no amino acid supplementation were tested. At the end of the trial, we found that fry fed diets, replacing 25 and 50% of fishmeal with bacterial SCP, were 9.1 and 21.8% heavier, respectively, than those fed the control diet (p < 0.05), while Feed Conversion Ratio (FCR) values were also lower in comparison to the reference group. These results were also supported by Protein Efficiency Ratio (PER) and Lipid Efficiency Ratio (LER) values that improved in fish fed diets replacing 50% fishmeal by bacterial SCP. The inclusion of SCP enhanced Feed intake (FI) (p < 0.05), although FI was reduced at higher inclusion levels (>50%), which was associated to feed palatability. High levels of bacterial SCP (>50%) affected the muscular amino acid and fatty acid profiles, imbalances that were associated to their dietary content. The broken-line regression analysis using muscle DHA content and weight gain data showed that the maximum levels of fishmeal replacement by bacterial SCP were 46.9 and 52%, respectively.

This work aimed to determine the physicochemical and biochemical properties of trypsin from beluga Huso huso and sevruga Acipenser stellatus, two highly valuable sturgeon species. According to the results obtained from the methods of casein-zymogram and inhibitory activity staining, the molecular weight of trypsin for sevruga and beluga was 27.5 and 29.5 kDa, respectively. Optimum pH and temperature values for both trypsins were recorded at 8.5 and 55 °C by BAPNA (a specific substrate), respectively. The stability of both trypsins was well-preserved at pH values from 6.0 to 11.0 and temperatures up to 50 °C. TLCK and SBTI, two specific trypsin inhibitors, showed a significant inhibitory effect on the enzymatic activity of both trypsins (p < 0.05). The enzyme activity was significantly increased in the presence of Ca⁺² and surfactants and decreased by oxidizing agents, Cu⁺², Zn⁺², and Co⁺² (p < 0.05). However, univalent ions Na⁺ and K⁺ did not show any significant effect on the activity of both trypsins (p > 0.05). The results of our study show that the properties of trypsin from beluga and sevruga are in agreement with data reported in bony fish and can contribute to the clear understanding of trypsin activity in these primitive species.

A 60-day feeding trial was conducted to determine the effects of different levels of grape seed oil (GO) on growth performance, digestive enzyme activity, fillet proximate and fatty acid composition of rainbow trout (Oncorhynchus mykiss) juveniles (40.17 ± 0.04 g). Five experimental diets were formulated where fish oil (FO) was replaced with 0 (D1), 25 (D2), 50 (D3), 75 (D4) and 100 (D5) % GO. The growth performance was significantly improved with increasing GO levels up to 50% after which fish growth declined (P < .05). Fillet fatty acid composition was showed significantly highest n-6 PUFA levels with increasing GO in diets, while n-3 HUFA levels especially EPA and DHA were significantly decreased (P < .05). Fish fed on diets containing higher levels of GO revealed a decrease in α-amylase activity, whereas trypsin, total alkaline protease, and lipase activities increased significantly with increasing GO levels up to 50% and then decreased. Based on the findings of the present study, it could be concluded that GO could be included in diets up to 50% where it had the best performance over the other diets tested in the present experiment.