Knowledge of the sequencing of the 16S rRNA gene constitutes a true revolution in understanding the composition of the intestinal microbiota and its implication in health states. This study details microbial composition through next-generation sequencing (NGS) technology in children with anemia. Anemia is the most frequent hematological disorder that affects human beings. In Peru, it is one of the conditions that presents the most significant concern due to the adverse effects that cause it, such as delayed growth and psychomotor development, in addition to a deficiency in cognitive development.

Water is a finite resource. Its safety and cleanliness are highly important to meet current and future human needs. Compared to other resources, water represents a main factor to achieve development in several areas and leads to reach economic progress of a nation. However, in recent years, the excessive demographic and industrial growth has exacerbated water contamination. In this study, biodepuration process of domestic sewage (DS), textile effluents (TE) and acid mine drainage (AMD) is conducted using starch-based xerogel (XG) from potato (Solanum tuberosum) peels. Results showed that the treatment is effective to achieve the reduction of 5-day biochemical oxygen demand (BOD₅). The most important result was the achievement of heavy metals removal for the three components. Firstly, there was a reduction of barium, zinc, and cadmium (91, 60 and 46%, respectively) for raw AMD. Secondly, there was a reduction in the levels of zinc, aluminum, and barium (89, 86 and 64%, respectively) for TE biodepuration. Finally, results showed a reduction in zinc, iron and cadmium levels (81, 78 and 57%, respectively) for DS biodepuration.

Zinc (Zn) is a vital micronutrient with essential roles in biological processes like enzyme function, gene expression, and cell signaling. Disruptions in the cellular regulation of Zn²⁺ ions often lead to pathological states. Mammalian Zn transporters, such as ZIP11, play a key role in homeostasis of this ion. ZIP11 resides predominately in the nucleus and Golgi apparatus. Our laboratory reported a function of ZIP11 in maintaining nuclear Zn levels in HeLa cervical cancer cells. Analyses of cervical and ovarian cancer patients’ datasets identified four coding, single-nucleotide polymorphisms (SNPs) in SLC39A11, the gene that encodes ZIP11, correlating with disease severity. We hypothesized that these SNPs might translate to functional changes in the ZIP11 protein by modifying access to substrate availability. We also proposed that a metal binding site (MBS) in ZIP11 is crucial for transmembrane Zn²⁺ transport and required for maintenance of various pathogenic phenotypes observed in HeLa cells. Here, we investigated these claims by re-introducing single the SLC39A11 gene encoding for mutant residues associated with the SNPs, as well as MBS mutations into HeLa cells knocked down for the transporter. Some SNPs-encoding ZIP11 variants rescued Zn levels, proliferation, migration, and invasiveness of KD cells. Conversely, single MBS mutations mimicked the traits of KD cells, confirming the transporter's role in establishing and maintaining proliferative, migratory, and invasive traits. Overall, the intricate role of Zn in cellular dynamics and cancer progression underscores the significance of Zn transporters like ZIP11 in potential therapeutic interventions.

The presence of heavy metals in water represents a risk to the life of all species on the planet. Phytoremediation is an effective alternative to remove heavy metals from contaminated aqueous environments. In the present research, Chenopodium album L. was examined for the remediation of waters contaminated with Cd, Pb, and Hg. Studies were carried out in waters containing each metal separately (monometallic aqueous systems) and in mixtures (trimetallic aqueous systems). First, the adaptation of Chenopodium album to different concentrations of Hoagland’s nutrient solution (HNS) was evaluated, then, a phytotoxicity study was carried out to determine the appropriate concentrations of each metal to test the tolerance of the plant during the accumulation study, and finally, the bioaccumulation capacity of Chenopodium album for Cd, Pb, and Hg was evaluated. Chenopodium album showed tolerance to levels of 5 mg/L Hg and 10 mg/L Cd and Pb in 25% HNS. The bioaccumulation tests showed that Chenopodium album can remediate Cd, Pb, and Hg contaminated waters in both monometallic and trimetallic aqueous systems. These findings suggest important future applications in the food industry for the production of Chenopodium album as we demonstrate that this species adapts and grows in hydroponic media. In particular, the ability of Chenopodium album to adapt to extreme conditions could be exploited for further studies on phytoremediation of heavy metals in river water, irrigation water, wastewater, effluents, and mine tailings.

Copper (Cu) is an essential micronutrient that serves as a cofactor for redox enzymes but becomes toxic when unregulated. In bacteria, while Cu efflux systems are well characterized, mechanisms of Cu import remain poorly understood. Here, we characterize the major facilitator superfamily transporter CuiT (STM1486) as a key Cu importer in Salmonella enterica. Comparative genomics revealed that cuiT is evolutionarily conserved across Enterobacteriaceae, and structural modeling predicts a 12-transmembrane-helix architecture with conserved His, Met, and Cys residues suitable for Cu⁺ coordination. Functional analyses demonstrated that deletion of cuiT reduces intracellular Cu accumulation, slows Cu uptake kinetics, and diminishes expression of Cu-responsive genes, including copA, cueP, cueO, and golB. Conversely, overexpression of CuiT increases intracellular Cu but sensitizes cells to Cu stress, highlighting the need for tight regulation. Kinetic modeling indicates that CuiT mediates rapid Cu import, supporting larger intracellular Cu pools compared to Pseudomonas influx transporters. These findings position CuiT as a central component of the Salmonella Cu homeostasis network, linking Cu import to transcriptional regulation, redox balance, and stress adaptation. Our work provides mechanistic insights into bacterial Cu acquisition and suggests CuiT and associated pathways as potential targets for antimicrobial strategies.