The prevalence of nappy rash or napkin dermatitis in Thailand infants aged 1–24 months is 36.1%. Dexpanthenol-containing nappy creams are clinical validated and effective in treating napkin dermatitis. This study explores the real-world usage satisfaction and perceived effectiveness of a 5% Dexpanthenol-containing product as a preventive strategy for napkin dermatitis. Respondents were mothers with babies between 6–12 months of age, who purchased and used a 5% Dexpanthenol-containing product (Bepanthen^® Ointment) in the past one month. An online study collected respondents’ satisfaction with the product and its benefits and properties. A total of 300 respondents (52% frequent users and 48% as-needed users) completed the study. Over 90% of respondents reported that their babies experienced less nappy-related discomfort, crying, fidgeting and sleep disruption after applying the nappy care product. Almost all respondents (96%) were satisfied with the product. Notably, frequent users of the product reported higher rates of satisfaction than as-needed users (62% vs. 43%; p < 0.05). This high level of satisfaction was associated with the moisturizing properties and long-lasting skin protection provided by the dexpanthenol-containing product. Our findings from a real-world setting further validate the use of dexpanthenol-containing products as a preventive strategy for napkin dermatitis in Thai infants.