The gastrointestinal tract (GIT) is a complex, dynamic, and critical part of the body, which plays an important role in the digestion and absorption of ingested nutrients and excreting waste products of digestion. In addition, GIT also plays a vital role in preventing the entry of harmful substances and potential pathogens into the bloodstream. The gastrointestinal tract hosts a significant number of microbes, which throughout their metabolites, directly interact with the hosts. In modern intensive animal farming, many factors can disrupt GIT functions. As dietary nutrients and biologically active substances play important roles in maintaining homeostasis and eubiosis in the GIT, this review aims to summarize the current status of our knowledge on the most important areas.

Heat stress (HS) significantly affects the performance of pigs by its induced stressors such as inflammation, hypoxia and oxidative stress (OS), which mightily strain the intestinal integrity and function of pigs. As heat stress progresses, several mechanisms in the intestinal epithelium involved in the absorption of nutrients and its protective functions are altered. Changes in these mechanisms are mainly driven by cellular oxidative stress, which promotes disruption of intestinal homeostasis, leading to intestinal permeability, emphasizing intestinal histology and morphology with little possibility of recovering even after exposure to HS. Identification and understanding of these altered mechanisms are crucial for providing appropriate intervention strategies. Therefore, it is this papers’ objective to review the important components for intestinal integrity that are negatively affected by HS and its induced stressors. With due consideration to the amelioration of such effects through nutritional intervention, this work will also look into the capability of dietary antioxidants in mitigating such adverse effects and maintaining the intestine’s integrity and function upon the pigs’ exposure to high environmental temperature.

The physiological status of poultry can be disturbed by different stressors that may lead to oxidative stress conditions. Oxidative stress activates defense systems, which mitigates the adverse effects. Several lines of the poultry defense system exist, including enzyme systems such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and non-enzymatic antioxidants such as Glutathione (GSH). Methionine—a vital amino acid in poultry nutrition—plays a significant role in protein synthesis, transsulfuration, and transmethylation and is also involved in several biochemical pathway activations that can affect the antioxidant system. Therefore, this review aims to summarize the current knowledge on the role of methionine in poultry under heat stress or managing stress, on the antioxidants responsible for scavenging free radicals (GSH) and those responsible for detoxification (SOD, CAT, and GPx). Different levels of methionine supplementation above the requirement (up to 1% Met added on the basal diet) have been tested on the antioxidant status of poultry. It has been shown to improve the antioxidant status and reduce oxidative stress. The results of many experiments on poultry supplemented with diets of different methionine sources indicate that L-Met has good potential to stimulate the antioxidant status of poultry.

The aim of this exploratory work, because of the existing bias on the size of the sample and some of the sociodemographic characteristics of the participants, was to investigate the Eastern European consumers’ beliefs and attitudes toward animal welfare, to perform a cross-country segmentation analysis and to observe possible differences with their Western European counterparts. For this purpose, a survey was conducted with 5508 consumers from 13 Eastern European countries (Bosnia and Herzegovina, Bulgaria, Czech Republic, Croatia, North Macedonia, Hungary, Moldova, Poland, Romania, Serbia, Slovakia, Slovenia, and Ukraine) using a questionnaire with nine statements about consumers beliefs regarding animal welfare (aspects of management, ethical issues about animals, and consequences of animal welfare on meat quality and price), one statement about the willingness to pay more for meat produced under better welfare conditions, and four statements regarding attitudes toward animal welfare. Differences between countries were detected for all the statements. Moreover, three clusters of consumers were identified: one with consumers indifferent towards animal welfare; one with consumers concerned about animal welfare, but they believe it is difficult to achieve; and one with consumers concerned about animal welfare, and they believe it is possible to achieve it.

Heart rate is one of the gold standards used to assess the workload level and fitness of horses. However, when slight differences need to be detected, it is not sensitive enough. Therefore, the aim of this study was to test the effect of competition level and phase of exercise on the heart rate and heart rate variability parameters in show jumpers. Fourteen horses were examined competing on three different levels: 100 cm (n = 4), 120 cm (n = 6), and 130 cm (n = 4). The length of work (min); average and maximum heart rate; average, maximum and minimum RR intervals (ms); SD1 and SD2 (ms); RMSSD (ms) and pNN50 (%); VLF, LF, HF (%) were analyzed. The measurement was divided into four phases: warm-up, resting period, show jumping course riding, and cool-down. The level of the course had no significant effect on average and maximum heart rates throughout the entire exercise. The maximum RR interval, RMSSD, pNN50, SD1, and %VLF values were significantly different (p < 0.05) in horses competing at 100 cm height from those competing in the 120 cm group. The SD1 value was sensitive for the level of competition, while the SD2 parameter was sensitive for detecting exercise phases. In conclusion, heart rate variability parameters are more sensitive for detecting smaller differences in workload than heart rate alone in lower-level show jumpers.