The phytohormone auxin is essential for plant growth and development and YUCCA (YUC) proteins catalyze a rate-limiting step for endogenous auxin biosynthesis. Despite YUC family genes have been isolated from several species, systematic expression analyses of YUCs in response to abiotic stress are lacking and little is known about the function of YUC homologs in agricultural crops. Cucumber (Cucumis sativus L.) is a world cultivated vegetable crop with great economical and nutritional value. In this study, we isolated 10 YUC family genes (CsYUCs) from cucumber and explored their expression pattern under four types of stress treatments. Our data showed that CsYUC8 and CsYUC9 were specifically upregulated to elevate the auxin level under high temperature. CsYUC10b was dramatically increased but CsYUC4 was repressed in response to low temperature. CsYUC10a and CsYUC11 act against the upregulation of CsYUC10b under salinity stress, suggesting that distinct YUC members participate in different stress response and may even antagonize each other to maintain the proper auxin levels in cucumber. Further, CsYUC11 was specifically expressed in the male flower in cucumber and enhanced tolerance to salinity stress and regulated pedicel and stamen development through auxin biosynthesis in Arabidopsis.

Leaf size and flatness directly affect photosynthesis and are closely related to agricultural yield. The final leaf size and shape are coordinately determined by cell proliferation, differentiation, and expansion during leaf development. Lettuce (Lactuca sativa L.) is one of the most important leafy vegetables worldwide, and lettuce leaves vary in shape and size. However, the molecular mechanisms of leaf development in lettuce are largely unknown. In this study, we showed that the lettuce APETALA2 (LsAP2) gene regulates leaf morphology. LsAP2 encodes a transcriptional repressor that contains the conserved EAR motif, which mediates interactions with the TOPLESS/TOPLESS-RELATED (TPL/TPR) corepressors. Overexpression of LsAP2 led to small and crinkly leaves, and many bulges were seen on the surface of the leaf blade. LsAP2 physically interacted with the CINCINNATA (CIN)-like TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factors and inhibited their transcriptional activation activity. RNA sequencing analysis showed that LsAP2 affected the expression of auxin- and polarity-related genes. In addition, LsAP2 directly repressed the abaxial identity gene KANADI2 (LsKAN2). Together, these results indicate that LsAP2 regulates leaf morphology by inhibiting CIN-like TCP transcription factors and repressing LsKAN2, and our work provides insights into the regulatory mechanisms of leaf development in lettuce.

Lettuce (Lactuca sativa L.) is a very important leafy vegetable in China and is commonly grown using furrow irrigation. In order to improve production efficiency, greenhouse experiments were conducted at Experimental Station, China Agricultural University, Beijing, China using furrow irrigation (FI), micro-sprinkler irrigation (MS), plastic film mulching irrigation (PF) and a combined plastic film mulching–micro-sprinkler irrigation system (PF+MS) to study their effects on soil physical characteristics, water distribution, root morpho-physiological traits, nutrition absorption, lettuce yield and water use efficiency for a spring crop and autumn crop in 2015 (Fig 1). Root length, root surface area, and root density were significantly higher under PF and PF+MS than under FI. Moreover, these traits were higher under MS than under FI but these differences were not significant. The soluble protein, soluble sugar, and Vitamin C content of lettuce decreased in the order PF+MS > PF > MS > FI in both crops. In the spring crop, the biological yield of MS, PF, and PF+MS was 7.22%、36.77%、43.20% higher than FI, respectively. In the spring crop, biological water use efficiency (BWUE) of FI, MS, PF and PF+MS was 20.93, 25.24, 36.81 and 38.54 kg m⁻³, respectively. The BWUE of MS, PF, and PF+MS was 20.59%, 75.87% and 84.14% higher than FI. Economic water use efficiency (EWUE) of FI, MS, PF and PF+MS was 17.06, 21.31, 31.11 and 32.31 kg m⁻³, respectively. The EWUE of MS, PF, and PF+MS was 24.91%, 82.36% and 89.39% higher than FI, respectively. The autumn crop achieved a higher WUE than the spring crop. The results suggested that the combined plastic film mulching-micro-sprinkler irrigation was the most suitable irrigation approach for increasing lettuce yield.