The occurrence and spread of ARB due to high demand in poultry industries are of great public health concern. The widespread emergence of antibiotic resistance, particularly MDR among bacterial pathogens, poses challenges in clinical treatment.

The prevalence and persistent outbreaks of multidrug-resistant (MDR) Salmonella in low-income countries have received growing attention among the public and scientific community. Notably, the excessive use of antibiotics in chicken feed for the purpose of treatment or as prophylaxis in the poultry industry have led to a rising rate of antimicrobial resistance. Therefore, this study aimed to determine the presence of antimicrobial-resistant Salmonella species and its mobile genetic elements from soil and effluent samples of 33 randomly selected poultry farms in Selangor, Malaysia. Salmonella species were isolated on selective media (CHROMagar™ Salmonella). VITEK^® 2 system was used to identify the isolates and their antimicrobial susceptibility. Subsequently, eight isolates were subjected to the whole genome sequencing (WGS). Based on the results, Salmonella spp. was detected in 38.1% (24/63) of samples, with the highest resistance to ampicillin (62.5%), followed by ampicillin/sulbactam (50.0%) and ciprofloxacin (45.8%). Meanwhile, the identified serovars were Salmonella enterica subspecies enterica serovar Weltevreden (S. Weltevreden), S. Jedburgh, and S. Brancaster. The most prevalent resistance genes detected include qnrS1, bla_TEM-176, dfrA14, and tet(A). The IncX1 plasmid, with encoded resistance genes, was also detected in four isolates. Furthermore, mutations in the quinolone resistant-determining regions (QRDR) were discovered, specifically in the gyrA, gyrB, and parC genes. In short, surveillance such as continuous monitoring of antimicrobial resistance and emerging trends in resistance patterns through farm environmental samples could provide information to formulate public health interventions for effective infection prevention and disease control.

Background/Objectives: The increasing threat of antimicrobial resistance (AMR) to global public health urgently needs attention. Misuse of antimicrobials in sectors such as dairy farming has led to the emergence and spread of resistant bacteria and genes. This study investigated AMR patterns and profiles of Escherichia coli (E. coli) from various sources, including soil, effluent, cow dung, and milk. Methods: A total of 192 samples were collected, comprising environmental samples (soil and effluent), cow dung samples, and milk samples from eight dairy farms in Selangor, Malaysia. The spread plate method was employed to isolate E. coli, and all the isolates were subjected to Gram staining to identify Gram-negative, rod-shaped bacteria. The Vitek^® 2 system was used for E. coli identification and susceptibility testing. Results: The prevalence of E. coli identified in the eight farms was 66.1%. A total of 360 E. coli isolates were successfully isolated, and 19.7% of the isolates presented AMR with ampicillin exhibiting the highest resistance (18.3%), followed by trimethoprim–sulfamethoxazole (8.9%). Additionally, 8.9% of them were multidrug resistant, which could be divided into 16 patterns. For the extended spectrum beta-lactamase screening, nine isolates were positive. Conclusions: This finding emphasizes the rise in resistant isolates in the growing dairy industry and underscores the urgency of addressing the potential reservoir of AMR. Therefore, essential measures such as continuous surveillance and effective antimicrobial stewardship programs are crucial for regulating veterinary antimicrobial use. Research on the mechanisms driving the development and dissemination of AMR is imperative for addressing One Health concerns.

Antimicrobial resistance (AMR) poses a significant “One Health” challenge in the farming industry attributed to antimicrobial misuse and overuse, affecting the health of humans, animals, and the environment. Recognizing the crucial role of the environment in facilitating the transmission of AMR is imperative for addressing this global health issue. Despite its urgency, there remains a notable gap in understanding resistance levels in the environment. This scoping review aims to consolidate and summarize available evidence of AMR prevalence and resistance genes in dairy farm settings. This study was conducted following the PRISMA Extension checklist to retrieve relevant studies conducted in Asian countries between 2013 and 2023. An electronic literature search involving PubMed, ScienceDirect, Embase, and Scopus resulted in a total of 1126 unique articles that were identified. After a full-text eligibility assessment, 39 studies were included in this review. The findings indicate that AMR studies in dairy farm environments have primarily focused on selective bacteria, especially Escherichia coli and other bacteria such as Staphylococcus aureus, Klebsiella spp., and Salmonella spp. Antimicrobial resistance patterns were reported across 24 studies involving 78 antimicrobials, which predominantly consisted of gentamicin (70.8%), ampicillin (58.3%), and tetracycline (58.3%). This review emphasizes the current state of AMR in the environmental aspects of dairy farms across Asia, highlighting significant gaps in regional coverage and bacterial species studied. It highlights the need for broader surveillance, integration with antimicrobial stewardship, and cross-sector collaboration to address AMR through a One Health approach.

Concern about the zoonotic Hepatitis E virus (HEV) is rising. Since, food handlers are at greater risk in contracting HEV, the present study aims to determine awareness, knowledge, prevention practices against HEV, and immunization attitudes. A cross sectional study was conducted among 400 food handlers in Klang Valley, Malaysia from December 2021 to March 2022. A structured questionnaire was employed for data collection and analysis with Statistical Package for Social Science (SPSS) version 29. Approximately 4.5% of the respondents (18) reported having heard of HEV, while the median scores for the knowledge and practice domains were 0/10 and 1/5, respectively. A total of 316 (79%) respondents expressed willingness to obtain vaccination if made available. This study also found that those respondents who completed their tertiary education were significantly possessed better knowledge of the disease [odd ratio (OR) = 8.95, and 95% confidence interval (CI) 4.98-16.10]. Respondents with HEV awareness reported considerably better practices (OR = 8.24, 95% CI 1.72-39.63). Food handlers with one to five years of experience in the industry expressed notable willingness to take vaccination (OR = 7.71, 95% CI 1.79-33.18). Addressing poor HEV awareness and knowledge and poor practices against the disease is crucial in enlightening the policy makers about awareness among food handlers and general population. Nonetheless, a good immunization attitude, significant acceptance toward vaccination even with the vaccine being unavailable in Malaysia, and limited awareness of HEV highlight a promising development.

Background/Objectives: Antibiotic resistance is a growing public health concern. The One Health approach is essential in addressing antibiotic (AR) resistance. Therefore, this study aimed to determine AR among enterococcal isolates in dairy farms across various domains based on the emerging dairy industry. Methods: A total of 208 enterococcal isolates from the workers = 70, milk = 76, and environment = 62 of eight dairy farms in Selangor, Malaysia, were used in this study. The bacterial identification and antibiotic susceptibility testing (AST) were conducted utilising the Vitek-2 system. Results: Approximately 28% of the isolates exhibited susceptibility to all tested antibiotics. A relatively higher proportion of isolates demonstrated resistance to tetracycline, which was followed by erythromycin. The multidrug resistance (MDR) and multiple antibiotic resistance (MAR) index were low in this study. Conclusions: The studied dairy farms exhibited lower AR, MDR, and MAR index values. Nevertheless, ongoing surveillance is essential due to the recent expansion of the dairy farming industry.