Although most plastic pollution originates on land, current research largely remains focused on aquatic ecosystems. Studies pioneering terrestrial microplastic research have adapted analytical methods from aquatic research without acknowledging the complex nature of soil. Meanwhile, novel methods have been developed and further refined. However, methodical inconsistencies still challenge a comprehensive understanding of microplastic occurrence and fate in and on soil. This review aims to disentangle the variety of state-of-the-art sample preparation techniques for heterogeneous solid matrices to identify and discuss best-practice methods for soil-focused microplastic analyses. We show that soil sampling, homogenization, and aggregate dispersion are often neglected or incompletely documented. Microplastic preconcentration is typically performed by separating inorganic soil constituents with high-density salt solutions. Not yet standardized but currently most used separation setups involve overflowing beakers to retrieve supernatant plastics, although closed-design separation funnels probably reduce the risk of contamination. Fenton reagent may be particularly useful to digest soil organic matter if suspected to interfere with subsequent microplastic quantification. A promising new approach is extraction of target polymers with organic solvents. However, insufficiently characterized soils still impede an informed decision on optimal sample preparation. Further research and method development thus requires thorough validation and quality control with well-characterized matrices to enable robust routine analyses for terrestrial microplastics.

Microplastics are the new emerging pollutants ubiquitously detectable in aquatic and terrestrial ecosystems. Fate and behavior, as well as ecotoxicity, are of increasing environmental concern, particularly in sediments and soils as natural sinks. For a global environmental risk assessment, reliable and easy to apply analytical methods are mandatory to obtain comparable data. This is based on the isolation of microplastics out of the solid sample matrices prior to instrumental detection. Thus, this study provides an easy to apply approach for density separation. The technique emerged from a comparative study using different salt solutions to isolate conventional, and for the first time biodegradable, microplastics from different solid sample matrices, i.e., sand, artificial soil, and compost. Four solutions (water, sodium chloride, sodium hexametaphosphate, and sodium bromide) of different densities were applied followed by oxidizing digestion. Finally, the impact of the procedures on size and surface properties of microplastics was tested. Dependent on the sample matrix, the highest recovery rates of 87.3–100.3% for conventional polymers, and 38.2–78.2% for biodegradable polymers, were determined with sodium bromide. It could be shown that the type of solid sample matrix influences the recovery rates and has to be considered when choosing a sample preparation technique. Graphical abstract

Agricultural soils have been identified as potential reservoirs for plastic pollution, with adverse effects on soil properties. Primary sources of plastic input in agricultural landscapes are associated with the application of sewage sludge or compost. Understanding the sources and anticipated plastic content is crucial in mitigating plastic pollution in agricultural fields. This study presents one of the first investigations into the plastic content and other impurities, e.g. glass, of seven organic fertilizers (biowaste compost, digested pig slurry, sewage sludge compost, dry chicken manure, green waste compost, sewage sludge, and a mixed digestate comprising pig slurry, chicken manure, and 74 % renewable raw materials). Potentially visible foreign substances were assessed on the surface of each fertilizer pile. No impurities could be detected in digested pig slurry, chicken manure, and mixed digestate. For the remaining fertilizers, visible potential foreign substances were collected, cleaned, visually described, weighed, photographed, size measured, and chemically characterized using ATR-FTIR. The quantification revealed that plastic particles are the most abundant and are contained in all other fertilizers, in contrast to glass and metal. An increasing trend in plastic particle number per m²: green waste < biowaste < sewage sludge compost < sewage sludge, which is about 4 times greater in sewage sludge than in green waste compost, could be observed. However, sewage sludge compost has the largest plastic mass and surface area per square meter. This illustrates that sewage sludge compost application can be a significant entry pathway for visual plastics into agricultural soils.

Plastic pollution is increasingly recognized as a threat to terrestrial environments, particularly soil. As plastics enter agricultural, roadside, urban, n

Microplastics are the new emerging pollutants ubiquitously detectable in aquatic and terrestrial ecosystems. Fate and behavior as well as ecotoxicity are of increasing environmental concern particularly in sediments and soils as natural sinks. For a global environmental risk assessment reliable and easy to apply analytical methods are mandatory to obtain comparable data. This is based on the isolation of microplastics out of the solid sample matrices prior to instrumental detection. Thus, this study provides a validated approach for density separation, which emerged from a comparative study using different salt solutions to isolate conventional and for the first time biodegradable microplastics from different solid sample matrices, i.e., sand, artificial soil, and compost. Four solutions (water, sodium chloride, sodium hexametaphosphate and sodium bromide) of different densities were applied followed by oxidizing digestion. Finally, the impact of the procedures on size and surface properties of microplastics was tested. Dependent on the sample matrix, highest recovery rates of 87.3-100.3 % for conventional polymers and 38.2–78.2 % for biodegradable polymers were determined with sodium bromide. It could be shown that the type of solid sample matrix influences the recovery rates and has to be considered when choosing a sample preparation technique.