The environmental impacts of photoluminescence and light-emitting diode (LED) lighting technologies in horticulture are described in this paper. As a case study, the life cycle assessment (LCA) associated with the raw materials, air, water and natural resources of screw-based compact fluorescent lamps (CFLs) and screw-based horticultural LED lamps (abbreviated as H-LED) used for “night break” effect in chrysanthemum cultivation is considered. Instead of the unit of radiant power of lighting sources (lumens) used in human lighting, the photon flux (micromoles per second) of the plant light is used in this study. The results of the study show that the environmental impacts of the H-LED lighting technology are markedly less than the fluorescent lamps. Therefore, the shift to LED lighting technology in horticulture in general, and “night break” chrysanthemum cultivation in particular, brings about tremendous benefits not only for saving energy and improving crop quality, but also for protecting the air/climate, water, soil and natural resources.