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Ming Li
Institute of Environmental Health and Ecological Security, School of the Environment and Safety Engineering, Jiangsu University Zhenjiang China

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Research article
Published: 15 January 2021 in Journal of the Science of Food and Agriculture
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BACKGROUND The ultrasensitive monitoring strategy of zearalenone (ZEN) was essential and desired for food safety and human health. In this work, a coupling of gold nanoparticles‐DNA barcode and direct competitive immunoassay based real‐time PCR signal amplification (RT–IPCR) for ZEN close to the sensitivity of PCR‐like level was described and evaluated. RESULTS The RT–IPCR was benefited from the use of DNA barcode and RT–PCR detection strategy, thus event resulting in ultrasensitive and simplicity detection for ZEN. Under the optimal RT–IPCR, the linear range of detection was from 0.5 to 1000 pg mL‐1, the limit of detection (LOD) was 0.5 pg mL‐1, which was 400‐fold lower than the ELISA. The detection procedure was simplified and the detection time was shortened. The specificity, accuracy and precision of the RT–IPCR had been confirmed high performance. The ZEN‐positive contamination levels were from 0.056 to 152.12 ng g‐1 by the RT–IPCR, which demonstrated highly reliable by the LC‐MS/MS. CONCLUSION The proposed RT–IPCR could be as an alternative for detecting ZEN with the satisfactory ultrasensitivity, simpleness, cheapness and high‐throughput. This study could provide a strategy for the ultrasensitive detection of the small molecule with a simple and practical approach, which has significant popularizing value and application prospect. This article is protected by copyright. All rights reserved.

ACS Style

Ming Li; Xia Hong; Xuchun Qiu; Chuqin Yang; Yuhao Mao; Yan Li; Zhenjiang Liu; Daolin Du. Ultrasensitive monitoring strategy of PCR ‐like levels for zearalenone contamination based DNA barcode. Journal of the Science of Food and Agriculture 2021, 1 .

AMA Style

Ming Li, Xia Hong, Xuchun Qiu, Chuqin Yang, Yuhao Mao, Yan Li, Zhenjiang Liu, Daolin Du. Ultrasensitive monitoring strategy of PCR ‐like levels for zearalenone contamination based DNA barcode. Journal of the Science of Food and Agriculture. 2021; ():1.

Chicago/Turabian Style

Ming Li; Xia Hong; Xuchun Qiu; Chuqin Yang; Yuhao Mao; Yan Li; Zhenjiang Liu; Daolin Du. 2021. "Ultrasensitive monitoring strategy of PCR ‐like levels for zearalenone contamination based DNA barcode." Journal of the Science of Food and Agriculture , no. : 1.

Research article
Published: 05 September 2020 in Journal of the Science of Food and Agriculture
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BACKGROUND The toxicity and health risks of mycotoxins have encouraged increased awareness and strict monitoring of these contaminants in agricultural byproducts. In this paper, we developed and tested a sensitive, selective, and self‐assembling immunochromatographic (IC) strip for on‐site detection of ochratoxin A (OTA). We were able to demonstrate semi‐quantitative and quantitative detection of OTA in agricultural byproduct samples. RESULTS The optimized IC strip had a limit of detection (LOD) of 0.5 ng/mL OTA using the naked eye for semi‐quantitative detection. When a digitized strip reader was used to achieve quantitative results, the LOD for OTA was reduced to 0.1 ng/mL with a linear detection range of 0.1–10 ng/mL. We also evaluated the specificity, stability, accuracy, and precision of the IC strip and demonstrated high performance in all of these areas. We then confirmed the ability of the IC strip to visually detect OTA contamination in authentic agricultural byproduct samples from the markets in China. Furthermore, quantitative detection of OTA using the IC strip showed that the concentration of OTA ranged from 1.7 to 101.3 ng/g in the positive agricultural byproduct samples, correlating well with the measurements obtained via liquid chromatography with tandem mass spectrometry. CONCLUSION The results indicated that this proposed IC strip was capable of sensitive, semi‐quantitative, quantitative, and on‐site detection of OTA contamination in agricultural byproduct samples. This article is protected by copyright. All rights reserved.

ACS Style

Yuhao Mao; Mingna Sun; Xia Hong; Sagnik Chakraborty; Jinsheng Duan; Ming Li; Daolin Du. Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip. Journal of the Science of Food and Agriculture 2020, 101, 1659 -1665.

AMA Style

Yuhao Mao, Mingna Sun, Xia Hong, Sagnik Chakraborty, Jinsheng Duan, Ming Li, Daolin Du. Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip. Journal of the Science of Food and Agriculture. 2020; 101 (4):1659-1665.

Chicago/Turabian Style

Yuhao Mao; Mingna Sun; Xia Hong; Sagnik Chakraborty; Jinsheng Duan; Ming Li; Daolin Du. 2020. "Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip." Journal of the Science of Food and Agriculture 101, no. 4: 1659-1665.

Journal article
Published: 14 August 2020 in Toxins
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Zearalenone (ZEN) is a prevalent mycotoxin that needs intensive monitoring. A semi-quantitative and quantitative immunochromatographic assay (ICA) was assembled for investigating ZEN contamination in 187 samples of cereal and their products from China in 2019. The semi-quantitative detection model had a limit of detection (LOD) of 0.50 ng/mL with visual judgment and could be completely inhibited within 5 min at 3.0 ng/mL ZEN. The quantitative detection model had a lower LOD of 0.25 ng/mL, and ZEN could be accurately and digitally detected from 0.25–4.0 ng/mL. The ICA method had a high sensitivity, specificity, and accuracy for on-site ZEN detection. For investigation of the authentic samples, the ZEN-positive rate was 62.6%, and the ZEN-positive levels ranged from 2.7 to 867.0 ng/g, with an average ZEN-positive level being 85.0 ng/g. Of the ZEN-positive samples, 6.0% exceeded the values of the limit levels. The ZEN-positive samples were confirmed to be highly correlated using LC-MS/MS (R2 = 0.9794). This study could provide an efficiency and accuracy approach for ZEN in order to achieve visual and digitized on-site investigation. This significant information about the ZEN contamination levels might contribute to monitoring mycotoxin occurrence and for ensuring food safety.

ACS Style

Xia Hong; Yuhao Mao; Chuqin Yang; Zhenjiang Liu; Ming Li; Daolin Du. Contamination of Zearalenone from China in 2019 by a Visual and Digitized Immunochromatographic Assay. Toxins 2020, 12, 521 .

AMA Style

Xia Hong, Yuhao Mao, Chuqin Yang, Zhenjiang Liu, Ming Li, Daolin Du. Contamination of Zearalenone from China in 2019 by a Visual and Digitized Immunochromatographic Assay. Toxins. 2020; 12 (8):521.

Chicago/Turabian Style

Xia Hong; Yuhao Mao; Chuqin Yang; Zhenjiang Liu; Ming Li; Daolin Du. 2020. "Contamination of Zearalenone from China in 2019 by a Visual and Digitized Immunochromatographic Assay." Toxins 12, no. 8: 521.

Journal article
Published: 01 August 2019 in Toxins
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In total, 405 samples of corn, corn products, and swine feed from China in 2016–2018 were surveyed for zearalenone (ZEN) contamination using a magnetic bead immunoassay-coupled biotin–streptavidin system (BAS-MBI). The developed BAS-MBI had a limit of detection (LOD) of 0.098 ng mL−1, with half-maximal inhibition concentration (IC50) of 0.71 ng mL−1 in working buffer, and an LOD of 0.98 ng g−1; the detection range was from 0.98 to 51.6 ng g−1 in authentic agricultural samples. The BAS-MBI has been demonstrated to be a powerful method for the rapid, sensitive, specific, and accurate determination of ZEN. The ZEN positivity rate reached the highest level of 40.6% in 133 samples in 2016; ZEN levels ranged from 1.8 to 1100.0 ng g−1, with an average level of 217.9 ng g−1. In 2017, the ZEN positivity rate was the lowest at 24.5% in 143 samples; ZEN levels ranged from 1.1 to 722.6 ng g−1, with an average of 166.7 ng g−1. In 2018, the ZEN positivity rate was 31.8% in 129 samples; ZEN levels ranged from 1.3 to 947.8 ng g−1, with an average of 157.0 ng g−1. About 20% of ZEN-positive samples exceeded maximum limit levels. An alternative method of ZEN detection and a valuable reference for ZEN contamination in corn and its related products in China are provided. This survey suggests the need for prevention of serious ZEN contamination, along with management for food safety and human health.

ACS Style

Ming Li; Chuqin Yang; Yuhao Mao; Xia Hong; Daolin Du. Zearalenone Contamination in Corn, Corn Products, and Swine Feed in China in 2016–2018 as Assessed by Magnetic Bead Immunoassay. Toxins 2019, 11, 451 .

AMA Style

Ming Li, Chuqin Yang, Yuhao Mao, Xia Hong, Daolin Du. Zearalenone Contamination in Corn, Corn Products, and Swine Feed in China in 2016–2018 as Assessed by Magnetic Bead Immunoassay. Toxins. 2019; 11 (8):451.

Chicago/Turabian Style

Ming Li; Chuqin Yang; Yuhao Mao; Xia Hong; Daolin Du. 2019. "Zearalenone Contamination in Corn, Corn Products, and Swine Feed in China in 2016–2018 as Assessed by Magnetic Bead Immunoassay." Toxins 11, no. 8: 451.

Journal article
Published: 24 December 2018 in Toxins
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A total of 328 agricultural product samples highly suspected to be contaminated, from flour companies, feed companies, and livestock farms throughout China, were surveyed for deoxynivalenol (DON) contamination using a self-assembly enzyme-linked immunosorbent assay (ELISA) kit. An ELISA kit for DON was developed with a 4.9 ng mL−1 limit of detection (LOD) in working buffer and a 200 ng g−1 LOD in authentic samples. The DON contamination detection rate was 88.7%, concentrations ranged from 200.9 to 6480.6 ng g−1, and the highest DON contamination was found in distillers’ dried grains with solubles with an average of 3204.5 ng g−1. Wheat bran and wheat were found to be the most commonly contaminated samples, and the corn meal samples had the lowest average DON level. This ELISA kit is a powerful alternative method for the rapid, sensitive, specific, accurate, and high-throughput determination of DON and can meet the maximum requirement levels. This survey suggests that DON contamination in the Chinese market is serious, and the contamination risk deserves attention. Essential preventive measures should be implemented to ensure food safety and human health.

ACS Style

Ming Li; Mingna Sun; Xia Hong; Jinsheng Duan; Daolin Du. Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit. Toxins 2018, 11, 6 .

AMA Style

Ming Li, Mingna Sun, Xia Hong, Jinsheng Duan, Daolin Du. Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit. Toxins. 2018; 11 (1):6.

Chicago/Turabian Style

Ming Li; Mingna Sun; Xia Hong; Jinsheng Duan; Daolin Du. 2018. "Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit." Toxins 11, no. 1: 6.

Journal article
Published: 06 October 2016 in Food Control
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Lanthanide ions, the excellent fluorescence markers, have the potential to simplify the performance of multi-analytes analysis. In this work, a powerful time-resolved fluoroimmunoassay (TRFIA) based on dual-labeled format were performed for detecting clenbuterol and salbutamol simultaneously in water and swine feed samples. Europium (Eu3+)-labeled goat anti-mouse IgG and Samarium (Sm3+)-labeled goat anti-rabbit IgG were prepared and used as fluorescent probes. Under optimal conditions, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC10) of the TRFIA were 0.263 and 0.007 ng/mL for clenbuterol, and 0.179 and 0.014 ng/mL for salbutamol, respectively. The cross-reactivities of the TRFIA between these two β2-agonists and with their analogues were negligible, thus indicated high specificity and promoted the simultaneous determination. The spiked recoveries were 72.2%–102.2% with relative standard deviations (RSDs) of 3.4–13.8% for clenbuterol and salbutamol in water and swine feed samples. Furthermore, the results of TRFIA correlated well with those obtained by HPLC. The proposed dual-labeled TRFIA was a satisfactory tool for simultaneous, sensitive, efficient and labour-saving detection of clenbuterol and salbutamol in water and swine feed samples.

ACS Style

Ming Li; Yuanyuan Zhang; Yonglai Xue; Xia Hong; Yin Cui; Zhenjiang Liu; Daolin Du. Simultaneous determination of β 2 -agonists clenbuterol and salbutamol in water and swine feed samples by dual-labeled time-resolved fluoroimmunoassay. Food Control 2016, 73, 1039 -1044.

AMA Style

Ming Li, Yuanyuan Zhang, Yonglai Xue, Xia Hong, Yin Cui, Zhenjiang Liu, Daolin Du. Simultaneous determination of β 2 -agonists clenbuterol and salbutamol in water and swine feed samples by dual-labeled time-resolved fluoroimmunoassay. Food Control. 2016; 73 ():1039-1044.

Chicago/Turabian Style

Ming Li; Yuanyuan Zhang; Yonglai Xue; Xia Hong; Yin Cui; Zhenjiang Liu; Daolin Du. 2016. "Simultaneous determination of β 2 -agonists clenbuterol and salbutamol in water and swine feed samples by dual-labeled time-resolved fluoroimmunoassay." Food Control 73, no. : 1039-1044.