Nitrogen (N) is important for the growth of crops. Estimating leaf nitrogen concentration (LNC) accurately and nondestructively is important for precision agriculture, reduces environmental pollution, and helps model global carbon and N cycles. Leaf reflectance, especially in the visible and near-infrared regions, has been identified as a useful indicator of LNC. Except reflectance passively acquired by spectrometers, the newly developed multispectral LiDAR and hyperspectral LiDAR provide possibilities for measuring leaf spectra actively. The regression relationship between leaf reflectance spectra and rice (Oryza sativa) LNC relies greatly on the algorithm adopted. It would be preferable to find one algorithm that performs well with respect to passive and active leaf spectra. Thus, this study assesses the influence of six popular linear and nonlinear methods on rice LNC retrieval, namely, partial least-square regression, least squares boosting, bagging, random forest, back-propagation neural network (BPNN), and support vector regression of different types/kernels/parameter values. The R², root mean square error and relative error in rice LNC estimation using these different methods were compared through the passive and active spectral measurements of rice leaves of different varieties at different locations and time (Yongyou 4949, Suizhou, 2014, Yangliangyou 6, Wuhan, 2015). Results demonstrate that BPNN provided generally satisfactory performance in estimating rice LNC using the three kinds of passive and active reflectance spectra.

Target classification techniques using spectral imagery and light detection and ranging (LiDAR) are widely used in many disciplines. However, none of the existing methods can directly capture spectral and 3D spatial information simultaneously. Multispectral LiDAR was proposed to solve this problem as its data combines spectral and 3D spatial information. Point-based classification experiments have been conducted with the use of multispectral LiDAR; however, the low signal to noise ratio creates salt and pepper noise in the spectral-only classification, thus lowering overall classification accuracy. In our study, a two-step classification approach is proposed to eliminate this noise during target classification: routine classification based on spectral information using spectral reflectance or a vegetation index, followed by neighborhood spatial reclassification. In an experiment, a point cloud was first classified with a routine classifier using spectral information and then reclassified with the k-nearest neighbors (k-NN) algorithm using neighborhood spatial information. Next, a vegetation index (VI) was introduced for the classification of healthy and withered leaves. Experimental results show that our proposed two-step classification method is feasible if the first spectral classification accuracy is reasonable. After the reclassification based on the k-NN algorithm was combined with neighborhood spatial information, accuracies increased by 1.50–11.06%. Regarding identification of withered leaves, VI performed much better than raw spectral reflectance, with producer accuracy increasing from 23.272% to 70.507%.

The abilities of multispectral LiDAR (MSL) as a new high-potential active instrument for remote sensing have not been fully revealed. This study demonstrates the potential of using the spectral and spatial features derived from a novel MSL to discriminate surface objects. Data acquired with the MSL include distance information and the intensities of four wavelengths at 556, 670, 700, and 780 nm channels. A support vector machine was used to classify diverse objects in the experimental scene into seven types: wall, ceramic pots, Cactaceae, carton, plastic foam block, and healthy and dead leaves of E. aureum. Different features were used during classification to compare the performance of different detection systems. The spectral backscattered reflectance of one wavelength and distance represented the features from an equivalent single-wavelength LiDAR system; reflectance of the four wavelengths represented the features from an equivalent multispectral image with four bands. Results showed that the overall accuracy of using MSL data was as high as 88.7%, this value was 9.8%–39.2% higher than those obtained using a single-wavelength LiDAR, and 4.2% higher than for multispectral image.

Nitrogen is an essential nutrient element in crop photosynthesis and yield improvement. Thus, it is urgent and important to accurately estimate the leaf nitrogen contents (LNC) of crops for precision nitrogen management. Based on the correlation between LNC and reflectance spectra, the hyperspectral LiDAR (HSL) system can determine three-dimensional structural parameters and biochemical changes of crops. Thereby, HSL technology has been widely used to monitor the LNC of crops at leaf and canopy levels. In addition, the laser-induced fluorescence (LIF) of chlorophyll, related to the histological structure and physiological conditions of green plants, can also be utilized to detect nutrient stress in crops. In this study, four regression algorithms, support vector machines (SVMs), partial least squares (PLS) and two artificial neural networks (ANNs), back propagation NNs (BP-NNs) and radial basic function NNs (RBF-NNs), were selected to estimate rice LNC in booting and heading stages based on reflectance and LIF spectra. These four regression algorithms were used for 36 input variables, including the reflectance spectral variables on 32 wavelengths and four peaks of the LIF spectra. A feature weight algorithm was proposed to select different band combinations for the LNC retrieval models. The determination coefficient (R²) and the root mean square error (RMSE) of the retrieval models were utilized to compare their abilities of estimating the rice LNC. The experimental results demonstrate that (I) these four regression methods are useful for estimating rice LNC in the order of RBF-NNs > SVMs > BP-NNs > PLS; (II) The LIF data in two forms, including peaks and indices, display potential in rice LNC retrieval, especially when using the PLS regression (PLSR) model for the relationship of rice LNC with spectral variables. The feature weighting algorithm is an effective and necessary method to determine appropriate band combinations for rice LNC estimation.