Visual inspection becomes very important part of mechatronic and industry applications. The methods which replace human eye and human factor increase speed and reliability of many processes. Virtual instrumentation (LabVIEW from National Instruments e.g.) is very good environment for developing visual inspection tools integrating all basic elements: imaging sensors, I/O hardware and algorithms [1]. Paper focuses on possibilities of designing automated visual inspection link based on LabVIEW for searching and counting of selected parts of products. Algorithms such as Pattern and Geometric Matching, Color Location or Hough Transformation are key elements of all software solutions. Designed visual systems also serves like client communicating with superior system (server) via TCP IP enabling remote control
1 April 2016 in Computer Methods and Programs in Biomedicine
There is no direct evidence for the exact cilia-inhibitory effects of opioids, which are generally used to achieve general anesthesia in combination with other anesthetic drugs. These are the reasons, why we analysed direct concentration-dependent or systemic effects of anesthetics (propofol, sufentanil, and midazolam) at a recommended doses administered individually or simultaneously on the tracheal ciliary beat frequency (CBF) in in vitro experimental conditions. Brush biopsy technique was used to remove the tracheal epithelia of guinea pigs for microscopy evaluation of ciliary beating monitored by high-speed video camera and analysed by Ciliary Analysis software. The tracheal CBF was significantly lower in the presence of sufentanil (10-8 mol/L) than in the control group; similarly for midazolam-sufentanil (10-8 - 10-5 mol/L), as well as for midazolam-propofol (10-5 and 10-3 mol/L) combinations. The fact that concurrent administration of benzodiazepine significantly increased the risk of sufentanil-induced cilia-inhibition was pharmacologically confirmed using GABAA receptor antagonist, bicuculline methiodide. The benefit of propofol on the potent cilia-inhibitory effect achieved by benzodiazepine-opioid combination was non-significant. We highlight the pharmacodynamics interaction between anesthetic drugs mediated via GABAA receptor with negative impact on the CBF in a respiratory epithelium under experimental condition rather than the effect of individual anesthetic.
This paper focuses on main principles of high speed imaging used for motion analysis of microscopic biomechanical objects represented by cilia of respiratory apparatus epithelium. Frequency based analysis (FFT and its derivates) is used for searching regions with dominant movement which brings more objectivity for acquired videosequences analysis and segmentation. 3D image information (videosequence) is projected to 2D frequency maps for evaluation. Paper brings basic mathematical apparatus and elementary approaches how to solve this tasks. Results of experiments are successfully built-in to medical and pharmacological research