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Dr. Mike Kaminiaris
Agricultural University of Athens

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0 Modelling
0 bacteria
0 Aspergillus
0 biocontrol
0 Aflatoxins

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Journal article
Published: 30 November 2020 in Chemosensors
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Aflatoxins are highly toxic fungal secondary metabolites that often contaminate food and feed commodities. An electrochemical immunosensor for the determination of aflatoxin B1 (AFB1) was fabricated by immobilizing monoclonal AFB1 antibodies onto a screen-printed gold electrode that was modified with carbo-methyldextran by N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide cross-linking. An electrochemical interfacial modelling of biomolecular recognition was suggested and reasonably interpreted. Impedance technology was employed for the quantitative determination of AFB1. The limit of detection concentration of AFB1 for standard solutions and spiked pistachio samples was 0.5 ng/mL and 1 ng/mL, respectively. The immunosensor was able to successfully determine AFB1 concentrations in the range of 4.56–50.86 ng/mL in unknown pistachio samples. Comparative chromatographic analysis revealed that AFB1 concentrations that were higher than 345 ng/mL were not within the immunosensor’s upper limits of detection. Selectivity studies against Ochratoxin A and Aflatoxin M1 demonstrated that the proposed AFB1 immunosensor was able to differentiate between these other fungal mycotoxins. The novel electrochemical immunosensor approach has the potential for rapid sample screening in a portable, disposable format, thus contributing to the requirement for effective prevention and the control of aflatoxin B1 in pistachios.

ACS Style

Michail D. Kaminiaris; Sophie Mavrikou; Maria Georgiadou; Georgia Paivana; Dimitrios I. Tsitsigiannis; Spyridon Kintzios. An Impedance Based Electrochemical Immunosensor For Aflatoxin B1 Monitoring in Pistachio Matrices. Chemosensors 2020, 8, 121 .

AMA Style

Michail D. Kaminiaris, Sophie Mavrikou, Maria Georgiadou, Georgia Paivana, Dimitrios I. Tsitsigiannis, Spyridon Kintzios. An Impedance Based Electrochemical Immunosensor For Aflatoxin B1 Monitoring in Pistachio Matrices. Chemosensors. 2020; 8 (4):121.

Chicago/Turabian Style

Michail D. Kaminiaris; Sophie Mavrikou; Maria Georgiadou; Georgia Paivana; Dimitrios I. Tsitsigiannis; Spyridon Kintzios. 2020. "An Impedance Based Electrochemical Immunosensor For Aflatoxin B1 Monitoring in Pistachio Matrices." Chemosensors 8, no. 4: 121.

Journal article
Published: 10 July 2020 in Toxins
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In recent years, very many incidences of contamination with aflatoxin B1 (AFB1) in pistachio nuts have been reported as a major global problem for the crop. In Europe, legislation is in force and 12 μg/kg of AFB1 is the maximum limit set for pistachios to be subjected to physical treatment before human consumption. The goal of the current study was to develop a mechanistic, weather-driven model to predict Aspergillus flavus growth and the AFB1 contamination of pistachios on a daily basis from nut setting until harvest. The planned steps were to: (i) build a phenology model to predict the pistachio growth stages, (ii) develop a prototype model named AFLA-pistachio (model transfer from AFLA-maize), (iii) collect the meteorological and AFB1 contamination data from pistachio orchards, (iv) run the model and elaborate a probability function to estimate the likelihood of overcoming the legal limit, and (v) manage a preliminary validation. The internal validation of AFLA-pistachio indicated that 75% of the predictions were correct. In the external validation with an independent three-year dataset, 95.6% of the samples were correctly predicted. According to the results, AFLA-pistachio seems to be a reliable tool to follow the dynamic of AFB1 contamination risk throughout the pistachio growing season.

ACS Style

Michail D. Kaminiaris; Marco Camardo Leggieri; Dimitrios I. Tsitsigiannis; Paola Battilani. AFLA-PISTACHIO: Development of a Mechanistic Model to Predict the Aflatoxin Contamination of Pistachio Nuts. Toxins 2020, 12, 445 .

AMA Style

Michail D. Kaminiaris, Marco Camardo Leggieri, Dimitrios I. Tsitsigiannis, Paola Battilani. AFLA-PISTACHIO: Development of a Mechanistic Model to Predict the Aflatoxin Contamination of Pistachio Nuts. Toxins. 2020; 12 (7):445.

Chicago/Turabian Style

Michail D. Kaminiaris; Marco Camardo Leggieri; Dimitrios I. Tsitsigiannis; Paola Battilani. 2020. "AFLA-PISTACHIO: Development of a Mechanistic Model to Predict the Aflatoxin Contamination of Pistachio Nuts." Toxins 12, no. 7: 445.