The variation of substrate concentration in anode chamber directly affects the power generation efficiency and decontamination performance of microbial fuel cell (MFC). In this study, three concentrations of swine wastewater with 800 mg/L, 1600 mg/L and 2500 mg/L were selected as substrates, and the performance of MFC and response characteristics of anode microbial community were investigated. The results show that the concentration of a selected substrate is positively correlated with the output voltage of MFC and chemical oxygen demand (COD) removal rate. The microbial community diversity in the anode chamber and the performance of battery can be significantly affected when concentration changes in different ways, which helps to selectively cultivate the adaptable dominant bacteria to enhance the stability and decontamination performance of MFC. The community structure of anodic biofilm is mainly composed of Proteobacteria, Bacteroidetes, Firmicutes, Chloroflexi and Spirochaetae. These findings are meaningful to improve the treatment effects of swine wastewater and can help to find out the mechanism of varying concentration that influences the production of microorganisms in MFC.

Microbial fuel cells (MFCs) have emerged as a sustainable technology for wastewater treatment that has potential to recycle bioelectricity from livestock wastewater. The performance of MFCs is influenced by the synergistic effect of anode material with nearby microorganisms. In this study, three identical double-chambered MFCs with different anode carbon clothes using swine wastewater are established. The optimization mechanism of MFC performance is analyzed by anode characteristics, cell performance, and microbial community, respectively. The results show that the surface structure and properties of the anode carbon cloth can be obviously improved by the acid–heat-modified treatment. The community structure of anodic biofilm, which varied with different modification methods, was mainly dominated by Proteobacteria, Firmicutes, and Bacteroidetes. These findings demonstrate efficient and simple methods for improving the performance of MFCs based on swine wastewater and may help to explore the influence mechanism of different modified anodes on the exoelectrogens.