In recent years, with the development of the nanomaterials discipline, many new pesticide drug-carrying systems—such as pesticide nano-metal particles, nano-metal oxides, and other drug-carrying materials—had been developed and applied to pesticide formulations. Although these new drug-loading systems are relatively friendly to the environment, the direct exposure of many metal nanoparticles to the environment will inevitably lead to potential effects. In response to these problems, organic nanomaterials have been rapidly developed due to their high-quality biodegradation and biocompatibility. Most of these organic nanomaterials were mainly polysaccharide materials, such as chitosan, carboxymethyl chitosan, sodium alginate, β-cyclodextrin, cellulose, starch, guar gum, etc. Some of these materials could be used to carry inorganic materials to develop a temperature- or pH-sensitive pesticide drug delivery system. Herein, the pesticide drug-carrying system developed based on polysaccharide materials, such as chitosan, was referred to as the pesticide polymer drug-carrying system based on polysaccharide materials. This kind of drug-loading system could be used to protect the pesticide molecules from harsh environments, such as pH, light, temperature, etc., and was used to develop the function of a sustained release, targeted release of pesticides in the intestine of insects, and achieve the goal of precise application, reduction, and efficiency of pesticides. In this review, the recent progress in the field of polysaccharide-based polymer drug delivery systems for pesticides has been discussed, and suggestions for future development were proposed based on the current situation.

Berberine (Ber) is easy to synthesize and has a variety of biological and pharmacological activities. At present, the existing studies on berberine have focused predominantly on its antibacterial activity; its herbicidal activity is rarely reported. In addition, there are a number of preparations of berberine, which are not enough to solve its shortcomings of low solubility and biological activity and the difficult storage of berberine. Here, berberine was combined with carbon dots to obtain carbon dots-berberine (CDs-Ber) nano formulation. The fluorescence quenching results showed that the CDs-Ber nano drug delivery system was successfully constructed, and the fluorescence quenching mechanism of the two was static quenching. The bioassay results showed that CDs had no adverse effects on the growth of barnyard grass (Echinochloa crus-galli) and redroot pigweed (Amaranthus retroflexus), and had high biocompatibility. Berberine and CDs-Ber predominantly affected the root growth of barnyard grass and redroot pigweed and could enhance the growth inhibition effect on weeds, to some extent. The results of the protective enzyme system showed that both berberine and CDs-Ber could increase the activities of Superoxide dismutase (SOD), Peroxidase (POD), and Catalase (CAT) in barnyard grass, and CDs-Ber had a stronger stress effect on barnyard grass than berberine. The determination of the number of bacterial communities in the soil after the berberine and CDs-Ber treatments showed that there was no significant difference in the effects of the two, indicating that CDs-Ber would not have more negative impacts on the environment. The CDs-Ber nano formulation improved the biological activity of berberine, enhanced the herbicidal effect, and was relatively safe for soil colonies.

Understanding plant-insect interactions can help control the harm of herbivorous pests. According to transcriptome data, transcripts of Solanum lycopersicum responding to feeding by Tuta absoluta were screened for important endopeptidase inhibitors. These genes were annotated as serine-type endopeptidase inhibitors from the potato inhibitor I family, potato type II proteinase inhibitor family, and soybean trypsin inhibitor (Kunitz) family. Based on the analysis of expression patterns, Solyc09g084480.2, Solyc03g020080.2, Solyc03g098760.1, and Solyc01g009020.1 were identified as key genes in the defense system of S. lycopersicum. The major endopeptidase genes such as Tabs008250, Tabs007396, and Tabs005701 in the larval stages of T. absoluta were also detected as potential targets of the plant endopeptidase inhibitors. The interaction mode between these endopeptidase and endopeptidase inhibitors was predicted based on the protein structure construction. This study aims to reveal the molecular response of S. lycopersicum to feeding by T. absoluta with high throughput sequencing and bioinformatics analysis.

It has long been disputed whether Tetranychus cinnabarinus and Tetranychus urticae belong to the same genus, with T. cinnabarinus regarded as a red form of T. urticae. However, it is unclear why T. urticae and T. cinnabarinus have different body colors. Since carotenoids are responsible for the color of many organisms, the carotenoid profiles of T. cinnabarinus and T. urticae were compared by HPLC. There was no difference in carotenoid type, but T. cinnabarinus contained significantly more neoxanthin, astaxanthin, α-carotene, β-carotene, and γ-carotene, which may contribute to the deep red color. The transcriptome sequencing of both species identified 4079 differentially expressed genes (DEGs), of which 12 were related to carotenoid metabolism. RNA interference (RNAi) experiments demonstrated that silencing seven of these DEGs resulted in the different accumulation of carotenoid compounds in T. cinnabarinus and T. urticae. In addition, the body of T. urticae turned yellow after two days of feeding with UGT double-stranded RNAs and β-UGT small interfering RNAs. In conclusion, differences in the carotenoid profiles of T. urticae and T. cinnabarinus may be responsible for the different body colors.