MicroRNAs (miRNAs) are noncoding, small RNAs of 20–24 nucleotides (nt) and function critically at the post-transcriptional level to regulate gene expression through cleaving mRNA targets or interfering with translation of the target mRNAs. They are broadly involved in many biological processes in plants. The miR397 family in plants contains several conserved members either in 21-nt or in 22-nt that mainly target the laccase (LAC) genes functioning in lignin synthesis and are involved in the development of plants under various conditions. Recent findings showed that miR397b in Arabidopsis could also target to Casein Kinase II Subunit Beta 3 (CKB3) and mediate circadian regulation and plant flowering. This review aims to summarise recent updates on miR397 and provides the available basis for understanding the functional mechanisms of miR397 in plant growth and development regulation and in response to external adverse stimulation.

The plant-specific BURP family proteins play diverse roles in plant development and stress responses, but the function mechanism of these proteins is still poorly understood. Proteins in this family are characterized by a highly conserved BURP domain with four conserved Cys-His repeats and two other Cys, indicating that these proteins potentially interacts with metal ions. In this paper, an immobilized metal affinity chromatography (IMAC) assay showed that the soybean BURP protein SALI3-2 could bind soft transition metal ions (Cd²⁺, Co²⁺, Ni²⁺, Zn²⁺ and Cu²⁺) but not hard metal ions (Ca²⁺ and Mg²⁺) in vitro. A subcellular localization analysis by confocal laser scanning microscopy revealed that the SALI3-2-GFP fusion protein was localized to the vacuoles. Physiological indexes assay showed that Sali3-2-transgenic Arabidopsis thaliana seedlings were more tolerant to Cu²⁺ or Cd²⁺ stresses than the wild type. An inductively coupled plasma optical emission spectrometry (ICP-OES) analysis illustrated that, compared to the wild type seedlings the Sali3-2-transgenic seedlings accumulated more cadmium or copper in the roots but less in the upper ground tissues when the seedlings were exposed to excessive CuCl₂ or CdCl₂ stress. Therefore, our findings suggest that the SALI3-2 protein may confer cadmium (Cd²⁺) and copper (Cu²⁺) tolerance to plants by helping plants to sequester Cd²⁺ or Cu²⁺ in the root and reduce the amount of heavy metals transported to the shoots.