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Prof. Dr. Denis J Wright
Emeritus Professor of Pest Management, Department of Life Sciences, Imperial College London, Silwood Park Campus, Ascot, Berkshire SL5 7PY, UK

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0 Insecticides
0 IPM
0 Resistance
0 biocontrol
0 Multi-trophic interactions

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IPM

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Journal article
Published: 05 February 2021 in BMC Plant Biology
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Background The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants. The precise biological function of SDF2 is not clear, but its expression is required for innate immunity in plants. Here, we investigate whether a continuous expression of PxSDF2L1 in Nicotiana benthamiana can similarly confer resistance to plant pathogen, particularly, the black shank Phytophthora parasitica var. nicotianae. Results The N. benthamiana plants were inoculated with agrobacteria transformed with a PVX-based binary vector carrying the PxSDF2L1 gene; similar agroinoculation experiments with a PVX vector carrying the GFP gene were used for controls. In pot trials, agroinfected N. benthamiana plants constitutively expressing PxSDF2L1 showed a significant reduction of stem disease symptoms caused by the inoculation with P. parasitica, compared with controls. Conclusions We confirm a role of PxSDF2L1 in resistance to black shank, with a potential application to engineering active resistance against this oomycete in the commercial N. tabacum species and propose its evaluation in other crop families and plant pathogens.

ACS Style

Ivis Moran-Bertot; Lianet Rodríguez-Cabrera; Orlando Borras-Hidalgo; Siliang Huang; Yunchao Kan; Denis J. Wright; Camilo Ayra-Pardo. Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae. BMC Plant Biology 2021, 21, 1 -9.

AMA Style

Ivis Moran-Bertot, Lianet Rodríguez-Cabrera, Orlando Borras-Hidalgo, Siliang Huang, Yunchao Kan, Denis J. Wright, Camilo Ayra-Pardo. Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae. BMC Plant Biology. 2021; 21 (1):1-9.

Chicago/Turabian Style

Ivis Moran-Bertot; Lianet Rodríguez-Cabrera; Orlando Borras-Hidalgo; Siliang Huang; Yunchao Kan; Denis J. Wright; Camilo Ayra-Pardo. 2021. "Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae." BMC Plant Biology 21, no. 1: 1-9.

Journal article
Published: 19 June 2020 in Toxins
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The Vip3Aa insecticidal protein from Bacillus thuringiensis (Bt) is produced by specific transgenic corn and cotton varieties for efficient control of target lepidopteran pests. The main threat to this technology is the evolution of resistance in targeted insect pests and understanding the mechanistic basis of resistance is crucial to deploy the most appropriate strategies for resistance management. In this work, we tested whether alteration of membrane receptors in the insect midgut might explain the >2000-fold Vip3Aa resistance phenotype in a laboratory-selected colony of Heliothis virescens (Vip-Sel). Binding of 125I-labeled Vip3Aa to brush border membrane vesicles (BBMV) from 3rd instar larvae from Vip-Sel was not significantly different from binding in the reference susceptible colony. Interestingly, BBMV from Vip-Sel larvae showed dramatically reduced levels of membrane-bound alkaline phosphatase (mALP) activity, which was further confirmed by a strong downregulation of the membrane-bound alkaline phosphatase 1 (HvmALP1) gene. However, the involvement of HvmALP1 as a receptor for the Vip3Aa protein was not supported by results from ligand blotting and viability assays with insect cells expressing HvmALP1.

ACS Style

Daniel Pinos; Maissa Chakroun; Anabel Millán-Leiva; Juan Luis Jurat-Fuentes; Denis J. Wright; Patricia Hernández-Martínez; Juan Ferré. Reduced Membrane-Bound Alkaline Phosphatase Does Not Affect Binding of Vip3Aa in a Heliothis virescens Resistant Colony. Toxins 2020, 12, 409 .

AMA Style

Daniel Pinos, Maissa Chakroun, Anabel Millán-Leiva, Juan Luis Jurat-Fuentes, Denis J. Wright, Patricia Hernández-Martínez, Juan Ferré. Reduced Membrane-Bound Alkaline Phosphatase Does Not Affect Binding of Vip3Aa in a Heliothis virescens Resistant Colony. Toxins. 2020; 12 (6):409.

Chicago/Turabian Style

Daniel Pinos; Maissa Chakroun; Anabel Millán-Leiva; Juan Luis Jurat-Fuentes; Denis J. Wright; Patricia Hernández-Martínez; Juan Ferré. 2020. "Reduced Membrane-Bound Alkaline Phosphatase Does Not Affect Binding of Vip3Aa in a Heliothis virescens Resistant Colony." Toxins 12, no. 6: 409.

Validation study
Published: 05 September 2017 in Insect Science
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Multitoxin Bt‐crops expressing insecticidal toxins with different modes of action, for example, Cry and Vip, are expected to improve resistance management in target pests. While Cry1A resistance has been relatively well characterized in some insect species, this is not the case for Vip3A, for which no mechanism of resistance has yet been identified. Here we applied HT‐SuperSAGE to analyze the transcriptome of the gut tissue of tobacco budworm Heliothis virescens (F.) laboratory‐selected for Vip3Aa resistance. From a total of 1 324 252 sequence reads, 5 895 126‐bp tags were obtained representing 17 751 nonsingleton unique transcripts (UniTags) from genetically similar Vip3Aa‐resistant (Vip–Sel) and susceptible control (Vip–Unsel) strains. Differential expression was significant (≥2.5 fold or ≤0.4; P < 0.05) for 1989 sequences (11.2% of total UniTags), where 420 represented overexpressed (OE) and 1569 underexpressed (UE) genes in Vip–Sel. BLASTN searches mapped 419 UniTags to H. virescens sequence contigs, of which, 416 (106 OE and 310 UE) were unambiguously annotated to proteins in NCBI nonredundant protein databases. Gene Ontology distributed 345 of annotated UniTags in 14 functional categories with metabolism (including serine‐type hydrolases) and translation/ribosome biogenesis being the most prevalent. A UniTag homologous to a particular member of the REsponse to PAThogen (REPAT) family was found among most overexpressed, while UniTags related to the putative Vip3Aa‐binding ribosomal protein S2 (RpS2) were underexpressed. qRT‐PCR of a subset of UniTags validated the HT‐SuperSAGE data. This study is the first providing lepidopteran gut transcriptome associated with Vip3Aa resistance and a foundation for future attempts to elucidate the resistance mechanism.

ACS Style

Camilo Ayra-Pardo; Maria E. Ochagavía; Ben Raymond; Asim Gulzar; Lianet Rodríguez-Cabrera; Claudia Rodríguez De La Noval; Ivis Morán Bertot; Ryohei Terauchi; Kentaro Yoshida; Hideo Matsumura; Pilar Téllez Rodríguez; Daily Hernández Hernández; Orlando Borrás-Hidalgo; Denis J. Wright. HT‐SuperSAGE of the gut tissue of a Vip3Aa‐resistantHeliothis virescens(Lepidoptera: Noctuidae) strain provides insights into the basis of resistance. Insect Science 2017, 26, 479 -498.

AMA Style

Camilo Ayra-Pardo, Maria E. Ochagavía, Ben Raymond, Asim Gulzar, Lianet Rodríguez-Cabrera, Claudia Rodríguez De La Noval, Ivis Morán Bertot, Ryohei Terauchi, Kentaro Yoshida, Hideo Matsumura, Pilar Téllez Rodríguez, Daily Hernández Hernández, Orlando Borrás-Hidalgo, Denis J. Wright. HT‐SuperSAGE of the gut tissue of a Vip3Aa‐resistantHeliothis virescens(Lepidoptera: Noctuidae) strain provides insights into the basis of resistance. Insect Science. 2017; 26 (3):479-498.

Chicago/Turabian Style

Camilo Ayra-Pardo; Maria E. Ochagavía; Ben Raymond; Asim Gulzar; Lianet Rodríguez-Cabrera; Claudia Rodríguez De La Noval; Ivis Morán Bertot; Ryohei Terauchi; Kentaro Yoshida; Hideo Matsumura; Pilar Téllez Rodríguez; Daily Hernández Hernández; Orlando Borrás-Hidalgo; Denis J. Wright. 2017. "HT‐SuperSAGE of the gut tissue of a Vip3Aa‐resistantHeliothis virescens(Lepidoptera: Noctuidae) strain provides insights into the basis of resistance." Insect Science 26, no. 3: 479-498.

Journal article
Published: 03 September 2015 in Insect Molecular Biology
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The widespread and sustainable exploitation of the entomopathogen Bacillus thuringiensis (Bt) in pest control is threatened by the evolution of resistance. Although resistance is often associated with loss of binding of the Bt toxins to the insect midgut cells, other factors have been implicated. Here we used suppressive subtractive hybridization and gene expression suppression to identify additional molecular components involved in Bt‐resistance in Plutella xylostella. We isolated transcripts from genes that were differentially expressed in the midgut of larvae from a resistant population, following ingestion of a Bt kurstaki HD1 strain‐based commercial formulation (DiPel), and compared with a genetically similar susceptible population. Quantitative real‐time polymerase‐chain reaction (RT‐PCR) analysis confirmed the differential basal expression of a subset of these genes. Gene expression suppression of three of these genes (P. xylostella cyclin‐dependent kinase 5 regulatory subunit associated protein 1‐like 1, stromal cell‐derived factor 2‐like 1 and hatching enzyme‐like 1) significantly increased the pathogenicity of HD1 to the resistant population. In an attempt to link the multitude of factors reportedly influencing resistance to Bt with the well‐characterized loss of toxin binding, we also considered Bt‐resistance models in P. xylostella and other insects.

ACS Style

Camilo Ayra-Pardo; B. Raymond; A. Gulzar; Lianet Rodríguez-Cabrera; Ivis Morán‐Bertot; Neil Crickmore; Denis J. Wright. Novel genetic factors involved in resistance toBacillus thuringiensisinPlutella xylostella. Insect Molecular Biology 2015, 24, 589 -600.

AMA Style

Camilo Ayra-Pardo, B. Raymond, A. Gulzar, Lianet Rodríguez-Cabrera, Ivis Morán‐Bertot, Neil Crickmore, Denis J. Wright. Novel genetic factors involved in resistance toBacillus thuringiensisinPlutella xylostella. Insect Molecular Biology. 2015; 24 (6):589-600.

Chicago/Turabian Style

Camilo Ayra-Pardo; B. Raymond; A. Gulzar; Lianet Rodríguez-Cabrera; Ivis Morán‐Bertot; Neil Crickmore; Denis J. Wright. 2015. "Novel genetic factors involved in resistance toBacillus thuringiensisinPlutella xylostella." Insect Molecular Biology 24, no. 6: 589-600.

Journal article
Published: 01 January 2014 in BMC Biology
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Transgenic crops expressing Bt toxins have substantial benefits for growers in terms of reduced synthetic insecticide inputs, area-wide pest management and yield. This valuable technology depends upon delaying the evolution of resistance. The ‘high dose/refuge strategy’, in which a refuge of non-Bt plants is planted in close proximity to the Bt crop, is the foundation of most existing resistance management. Most theoretical analyses of the high dose/refuge strategy assume random oviposition across refugia and Bt crops.

ACS Style

Pilar Téllez-Rodríguez; Ben Raymond; Ivis Morán-Bertot; Lianet Rodríguez-Cabrera; Denis J Wright; Carlos G Borroto; Camilo Ayra-Pardo. Strong oviposition preference for Bt over non-Bt maize in Spodoptera frugiperda and its implications for the evolution of resistance. BMC Biology 2014, 12, 48 -48.

AMA Style

Pilar Téllez-Rodríguez, Ben Raymond, Ivis Morán-Bertot, Lianet Rodríguez-Cabrera, Denis J Wright, Carlos G Borroto, Camilo Ayra-Pardo. Strong oviposition preference for Bt over non-Bt maize in Spodoptera frugiperda and its implications for the evolution of resistance. BMC Biology. 2014; 12 (1):48-48.

Chicago/Turabian Style

Pilar Téllez-Rodríguez; Ben Raymond; Ivis Morán-Bertot; Lianet Rodríguez-Cabrera; Denis J Wright; Carlos G Borroto; Camilo Ayra-Pardo. 2014. "Strong oviposition preference for Bt over non-Bt maize in Spodoptera frugiperda and its implications for the evolution of resistance." BMC Biology 12, no. 1: 48-48.

Review
Published: 07 January 2013 in Annual Review of Entomology
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Agricultural intensification and greater production of Brassica vegetable and oilseed crops over the past two decades have increased the pest status of the diamondback moth (DBM), Plutella xylostella L., and it is now estimated to cost the world economy US$4-5 billion annually. Our understanding of some fundamental aspects of DBM biology and ecology, particularly host plant relationships, tritrophic interactions, and migration, has improved considerably but knowledge of other aspects, e.g., its global distribution and relative abundance, remains surprisingly limited. Biological control still focuses almost exclusively on a few species of hymenopteran parasitoids. Although these can be remarkably effective, insecticides continue to form the basis of management; their inappropriate use disrupts parasitoids and has resulted in field resistance to all available products. Improved ecological understanding and the availability of a series of highly effective selective insecticides throughout the 1990s provided the basis for sustainable and economically viable integrated pest management (IPM) approaches. However, repeated reversion to scheduled insecticide applications has resulted in resistance to these and more recently introduced compounds and the breakdown of IPM programs. Proven technologies for the sustainable management of DBM currently exist, but overcoming the barriers to their sustained adoption remains an enormous challenge.

ACS Style

Michael J. Furlong; Denis J. Wright; Lloyd M. Dosdall. Diamondback Moth Ecology and Management: Problems, Progress, and Prospects. Annual Review of Entomology 2013, 58, 517 -541.

AMA Style

Michael J. Furlong, Denis J. Wright, Lloyd M. Dosdall. Diamondback Moth Ecology and Management: Problems, Progress, and Prospects. Annual Review of Entomology. 2013; 58 (1):517-541.

Chicago/Turabian Style

Michael J. Furlong; Denis J. Wright; Lloyd M. Dosdall. 2013. "Diamondback Moth Ecology and Management: Problems, Progress, and Prospects." Annual Review of Entomology 58, no. 1: 517-541.

Journal article
Published: 29 April 2012 in Journal of Insect Behavior
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Understanding the factors influencing host-selection behavior of parasitoids is essential in studies on host-parasitoid ecology and evolution, and in combining sustainable strategies of pest management, such as host-plant resistance and biological control. The effects of host-plant resistance on the olfactory response and parasitism success by Cotesia vestalis, a parasitoid of diamondback moth (Plutella xylostella) larvae were examined. Here, it was demonstrated that host-plant resistance can strongly influence foraging behavior and parasitism success of the parasitoid. In olfactometer experiments, C. vestalis did not differentiate between crucifer plant types with similar levels of susceptibility or resistance to P. xylostella but showed a strong preference for susceptible compared with partially-resistant host plants. The influence of previous oviposition activity varied with the host-plant type experienced by the parasitoid. In cage experiments, C. vestalis preferred to parasitize P. xylostella larvae on a susceptible plant compared with larvae on a partially resistant host plant when exposed to hosts for 24 h. However, this preference appeared to be transitory, and was not found after 96 h exposure. The present study suggests that combining partial host-plant resistance with biological control by C. vestalis for the control of P. xylostella may in some circumstances be antagonistic and negatively affect parasitism success.

ACS Style

Javad Karimzadeh; Jim Hardie; Denis J. Wright. Plant Resistance Affects the Olfactory Response and Parasitism Success of Cotesia vestalis. Journal of Insect Behavior 2012, 26, 35 -50.

AMA Style

Javad Karimzadeh, Jim Hardie, Denis J. Wright. Plant Resistance Affects the Olfactory Response and Parasitism Success of Cotesia vestalis. Journal of Insect Behavior. 2012; 26 (1):35-50.

Chicago/Turabian Style

Javad Karimzadeh; Jim Hardie; Denis J. Wright. 2012. "Plant Resistance Affects the Olfactory Response and Parasitism Success of Cotesia vestalis." Journal of Insect Behavior 26, no. 1: 35-50.

Research article
Published: 01 January 2012 in Nematology
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ACS Style

Denis Wright. Perry, R.N. & Wharton, D.A. (Eds). Molecular and physiological basis of nematode survival. Wallingford, UK, CAB International, 2011, 320 pp. ISBN 978-1-84593-687-7. Price: £95.00, €135.00 or US$ 180.00. Nematology 2012, 14, 643 -644.

AMA Style

Denis Wright. Perry, R.N. & Wharton, D.A. (Eds). Molecular and physiological basis of nematode survival. Wallingford, UK, CAB International, 2011, 320 pp. ISBN 978-1-84593-687-7. Price: £95.00, €135.00 or US$ 180.00. Nematology. 2012; 14 (5):643-644.

Chicago/Turabian Style

Denis Wright. 2012. "Perry, R.N. & Wharton, D.A. (Eds). Molecular and physiological basis of nematode survival. Wallingford, UK, CAB International, 2011, 320 pp. ISBN 978-1-84593-687-7. Price: £95.00, €135.00 or US$ 180.00." Nematology 14, no. 5: 643-644.

Book chapter
Published: 16 November 2009 in Environmental impact of genetically modified crops
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From their introduction in 1996-2007, the genetically modified (GM) crops grown with traits for insecticide resistance (GM IR) have been...

ACS Style

B. Raymond; D. J. Wright. Resistance management of transgenic insect-resistant crops: ecological factors. Environmental impact of genetically modified crops 2009, 101 -114.

AMA Style

B. Raymond, D. J. Wright. Resistance management of transgenic insect-resistant crops: ecological factors. Environmental impact of genetically modified crops. 2009; ():101-114.

Chicago/Turabian Style

B. Raymond; D. J. Wright. 2009. "Resistance management of transgenic insect-resistant crops: ecological factors." Environmental impact of genetically modified crops , no. : 101-114.

Journal article
Published: 27 November 2007 in Ecological Entomology
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ACS Style

Javad Karimzadeh; Denis J. Wright. Bottom-up cascading effects in a tritrophic system: interactions between plant quality and host-parasitoid immune responses. Ecological Entomology 2007, 33, 45 -52.

AMA Style

Javad Karimzadeh, Denis J. Wright. Bottom-up cascading effects in a tritrophic system: interactions between plant quality and host-parasitoid immune responses. Ecological Entomology. 2007; 33 (1):45-52.

Chicago/Turabian Style

Javad Karimzadeh; Denis J. Wright. 2007. "Bottom-up cascading effects in a tritrophic system: interactions between plant quality and host-parasitoid immune responses." Ecological Entomology 33, no. 1: 45-52.

Journal article
Published: 01 September 2006 in Journal of Applied Entomology
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ACS Style

Hugo Cerda; A. H. Sayyed; D. J. Wright. Diamondback moth resistance to Bacillus thuringiensis transgenic canola: evaluation of refugia size with non-recessive resistant insects. Journal of Applied Entomology 2006, 130, 421 -425.

AMA Style

Hugo Cerda, A. H. Sayyed, D. J. Wright. Diamondback moth resistance to Bacillus thuringiensis transgenic canola: evaluation of refugia size with non-recessive resistant insects. Journal of Applied Entomology. 2006; 130 (8):421-425.

Chicago/Turabian Style

Hugo Cerda; A. H. Sayyed; D. J. Wright. 2006. "Diamondback moth resistance to Bacillus thuringiensis transgenic canola: evaluation of refugia size with non-recessive resistant insects." Journal of Applied Entomology 130, no. 8: 421-425.

Journal article
Published: 01 December 2004 in Journal of Economic Entomology
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ACS Style

A. H. Sayyed; D. J. Wright. Fipronil Resistance in the Diamondback Moth (Lepidoptera: Plutellidae): Inheritance and Number of Genes Involved. Journal of Economic Entomology 2004, 97, 2043 -2050.

AMA Style

A. H. Sayyed, D. J. Wright. Fipronil Resistance in the Diamondback Moth (Lepidoptera: Plutellidae): Inheritance and Number of Genes Involved. Journal of Economic Entomology. 2004; 97 (6):2043-2050.

Chicago/Turabian Style

A. H. Sayyed; D. J. Wright. 2004. "Fipronil Resistance in the Diamondback Moth (Lepidoptera: Plutellidae): Inheritance and Number of Genes Involved." Journal of Economic Entomology 97, no. 6: 2043-2050.

Comparative study
Published: 03 November 2003 in Pest Management Science
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This paper describes the assessment of nematode spray application methods and details the redesign of a spinning disc applicator for the improved application of the infective juveniles of entomopathogenic nematodes to foliage. The assessment highlighted fundamental design features that prevent efficient and accurate nematode application and these are dealt with in turn specifically to enable improved nematode application. The redesigned or prototype spinning disc was shown to improve nematode carriage in droplets, leading to greater deposition of nematodes per unit area. There is a trade‐off between spray droplet coverage of the target surface and the production of a suitable droplet spectrum for nematode carriage. Copyright © 2003 Society of Chemical Industry

ACS Style

Simon J Piggott; Richard Clayton; Graham A Matthews; Denis J Wright. Development of a new application apparatus for entomopathogenic nematodes. Pest Management Science 2003, 59, 1344 -1348.

AMA Style

Simon J Piggott, Richard Clayton, Graham A Matthews, Denis J Wright. Development of a new application apparatus for entomopathogenic nematodes. Pest Management Science. 2003; 59 (12):1344-1348.

Chicago/Turabian Style

Simon J Piggott; Richard Clayton; Graham A Matthews; Denis J Wright. 2003. "Development of a new application apparatus for entomopathogenic nematodes." Pest Management Science 59, no. 12: 1344-1348.

Journal article
Published: 01 April 2003 in Journal of Applied Entomology
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ACS Style

Hugo Cerda; A. H. Sayyed; D. J. Wright. Laboratory culture conditions affect stability of resistance to Bacillus thuringiensis Cry1Ac in Plutella xylostella (Lep., Plutellidae). Journal of Applied Entomology 2003, 127, 142 -145.

AMA Style

Hugo Cerda, A. H. Sayyed, D. J. Wright. Laboratory culture conditions affect stability of resistance to Bacillus thuringiensis Cry1Ac in Plutella xylostella (Lep., Plutellidae). Journal of Applied Entomology. 2003; 127 (3):142-145.

Chicago/Turabian Style

Hugo Cerda; A. H. Sayyed; D. J. Wright. 2003. "Laboratory culture conditions affect stability of resistance to Bacillus thuringiensis Cry1Ac in Plutella xylostella (Lep., Plutellidae)." Journal of Applied Entomology 127, no. 3: 142-145.