Water shortage and salinity are major challenges for sustaining global food security. Using nutrients in the nano-scale formulation including zinc oxide nanoparticles (ZnO NP) is a novel fertilization strategy for crops. In this study, two field-based trials were conducted during 2018 and 2019 to examine the influence of three ZnO NP concentrations (0, 50, and 100 ppm) in eggplant grown under full irrigation (100 of crop evapotranspiration; ETc) and drought stress (60% of ETc). Plant growth, yield, water productivity (WP), physiology, biochemistry, and anatomy responses were evaluated. Drought stress significantly decreased membrane stability index (MSI), relative water content (RWC), and photosynthetic efficiency, thus hampered eggplant growth and yield. In contrast, exogenous ZnO NP to water-stressed eggplant resulted in increased RWC and MSI associated with improved stem and leaf anatomical structures and enhanced photosynthetic efficiency. Under drought stress, supplementation of 50 and 100 ppm ZnO NP improved growth characteristics and increased fruit yield by 12.2% and 22.6%, respectively, compared with fully irrigated plants and nonapplied ZnO NP. The highest water productivity (WP) was obtained when eggplant was irrigated with 60% ETc and foliarly treated with 50 or 100 ppm of ZnO NP, which led to 50.8–66.1% increases in WP when compared with nontreated fully irrigated plants. Collectively, these findings demonstrated that foliar spraying ZnO NP gives the utility for alleviating drought stress effects on eggplant cultivated in saline soil.

Exogenous antioxidant applications enable salt-stressed plants to successfully cope with different environmental stresses. The objectives of this investigation were to study the effects of sequential treatments of proline (Pro), ascorbic acid (AsA), and/or glutathione (GSH) on 100 mM NaCl-stressed cucumber transplant’s physio-biochemical and growth traits as well as systems of antioxidant defense. Under salinity stress, different treatment of AsA, Pro, or/and GSH improved growth characteristics, stomatal conductance (gs), enhanced the activities of glutathione reductase (GR), superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) as well as increased contents of AsA, Pro, and GSH. However, sequential application of antioxidants (GSH-Pro- AsA) significantly exceeded all individual applications, reducing leaf and root Cd²⁺ and Na⁺ contents in comparison to the control. In plants grown under NaCl-salt stress, growth characteristics, photosynthetic efficiency, membrane stability index (MSI), relative water content (RWC), contents of root and leaf K⁺ and Ca²⁺, and ratios of K⁺/Na⁺ and Ca²⁺/Na⁺ were notably reduced, while leaf contents of non-enzymatic and enzymatic antioxidants, as well as root and leaf Cd²⁺ and Na⁺ concentrations were remarkably increased. However, AsA, Pro, or/and GSH treatments significantly improved all investigated growth characteristics, photosynthetic efficiency, RWC and MSI, as well as AsA, Pro, and GSH, and enzymatic activity, leaf and root K⁺ and Ca²⁺ contents and their ratios to Na⁺, while significantly reduced leaf and root Cd²⁺ and Na⁺ contents.

Phosphorus (P) is an essential macronutrient necessary for plant growth, development, and reproduction. Two field experiments were carried out in 2018/2019 and 2019/2020 on P-deficient soil to evaluate the impact of foliar fertilization with nanophosphorus (nP) on growth, yield, and physio-biochemical indices, as well as trigonelline content of fenugreek plants under deficient irrigation (dI) stress (a deficit of 20 and 40% of crop evapotranspiration; dI-20 and dI-40). The growth and yield traits, leaf integrity (relative water content and membrane stability index), photosynthetic pigment contents, leaf and seed P contents, and stem and leaf anatomical features significantly decreased under dI-20, with greater reductions recorded under dI-40. In contrast, water-use efficiency, osmoprotective compounds, including free amino acids, soluble sugars, proline, and trigonelline, along with antioxidant contents (ascorbate, glutathione, phenolics, and flavonoids) and their activity increased significantly under both dI-20 and dI-40. However, foliar feeding with nano-P considerably increased plant growth and yield traits, leaf integrity, photosynthetic pigments contents, leaf and seed P contents, and anatomical features. Besides, water-use efficiency, osmoprotectant contents, and antioxidant content and activity were further increased under both dI-20 and dI-40. The positive effects were more pronounced with the smaller nP (25 nm) than the larger nP (50 nm). The results of this study backed up the idea of using foliar nourishment with nP, which can be effective in modulating fenugreek plant growth and seed production.

Water deficit is a major growth limitation factor affecting plant growth and productivity. To counteract the harmful effects of drought stress, lupine plants were sprayed with two concentrations of algae extract (1 g L-1 and 2 g L-1) and hydrogen peroxide solution (0.5% and 1%) under low irrigation level. Morphological, physiological and anatomical studies were performed. It was found that all treatments enhanced plant height, root length, number of leaves, average leaf area, shoot and root fresh and dry weights. A significant increment in chlorophyll a and b, carotenoids and total pigments contents, as well as a significant decrease in electrolyte leakage was noted in treated plants compared with untreated under water deficit. Increment in levels of osmoprotectants such as proline, total free amino acids, glycine betaine and total soluble sugars contents was highly significant in case of 2 g L-1 algae extract treatment compared with untreated plants group. Anatomical studies of root, stem and leaf of treated and untreated plants revealed that 2 g L-1 algae extract treatment followed with 0.5% H2O2 induced significant increments in the thickness of the studied plant organs compared with the untreated plants under water deficit conditions. In general, the concentrations of 2 g L-1 algae extract and 0.5% H2O2 were the most effective doses regarding all the studied characteristics.

Drought stress is a major limitation to legume productivity in arid and semi-arid regions. This study investigated the effectiveness of two novel natural b