This is the first study to generate carrot plants for enhanced salinity tolerance using a single-cell in vitro system. Protoplasts of three carrot accessions were exposed to treatment by seven different concentrations of NaCl (10–400 mM). Salt concentrations higher than 50 mM decreased plating efficiency and those of 200–400 mM of NaCl completely arrested mitotic divisions of cultured cells. The protoplast-derived plants from the control and 50–100 mM NaCl treatment were subjected to an 8-week salt stress in greenhouse conditions induced by salinized soil (EC 3 and 6 mS cm⁻¹). 50 mM NaCl stress applied in vitro induced polyploidy among regenerated plants. The regenerants obtained from the 50 and 100 mM NaCl-treated protoplast cultures grown in saline soil had a higher survival rate compared to the regenerants from the control cultures. The salt-stressed plants accumulated anthocyanins in petioles and produced denser hairs on leaves and petioles in comparison to the control plants. Salt stress influenced pollen viability and seed setting of obtained regenerants. The results suggest that salt stress applied in vitro in protoplast cultures creates variation which allows alleviating the negative effects of salt stress on the development and reproduction of the carrot.

The common ice plant (Mesembryanthemum crystallinum L.) is a widely studied model due to its tolerance to numerous biotic and abiotic stresses. In this study, carried out in model pots, the plants were treated with variant doses of Cd(II) and Cr(VI) and proved resistant to extreme levels of these heavy metals. Initial toxicity symptoms were observed upon final concentrations of 818 mg Cd kg⁻¹ soil d.w., and 1699 mg Cr kg⁻¹ applied as potassium chromate. Biometric analyses revealed that none of the Cr(VI) doses affected dry weight of the plant organs thus maintaining the shoot-to-root ratio. The Cd and Cr hypertolerance strategies were divergent and resulted in different accumulation patterns. For the case of Cd(II), an excluder-like mechanism was developed to prevent the plant from toxicity. For chromate, high accumulation potential together with Cr(VI) root-to-shoot translocation at sublethal concentrations was revealed (up to 6152 mg Cr kg⁻¹ shoot at 4248 mg Cr kg⁻¹ soil). It is concluded that M. crystallinum reveals considerable phytoremediation capabilities due to unique growth potential in contaminated substrates and is suitable for bioreclamation of degraded soils. The plant is especially applicable for efficient phytoextraction of chromate-contamination, whereas for Cd-affected areas it may have a phytostabilizing effect.

Green roofs are becoming increasingly common practice of the urban sustainable environment. The growing substrate is the most important part of green-roof technology. The cost of engineering substrates can be reduced by using locally available components. Since green roofs are a relatively new concept in Poland, there is a need to examine substrate compositions and characteristics, including commonly used ingredients as well as alternative recycled/waste materials. The aim of our study was to assess the ability of locally sourced waste materials as roof-growing media amendments. In the greenhouse experiment we tested two grass and herb species mixtures and four waste substrate formulas. The locally disposed waste materials used as components of growing media included silica wastes (byproducts of metallic ferrosilicon alloys), cellulose, foundry sand, and organic waste material removed from the organic horizons of mucky peat. The engineered Si-waste substrates were compared with the commercially available media. The physico-chemical properties of components and substrates, their stability over time, and the influence on plant growth and mineral nutrient status were examined. Particle size distribution, bulk density, mass, water capacity, soil reaction, and total dissolved salt content of Si-waste-growing media were compatible with FLL standards. We found low amounts of available P and K, and high concentrations of Ca, Mg, S, and trace elements (with the exception of B) in Si-waste substrates in comparison with the control media. Silica waste materials have the potential to maintain pH with high buffering capacity. Engineered Si-waste substrates had a positive impact on plant growth and biomass. In general, these results indicate that contaminant elements contained in alkaline Si-waste substrates were not easily available to the root system, and consequently they did not restrict plant growth. We consider Si-wastes to be a valuable and environmentally responsible green roof media amendment.

Over the last decade, an increase in the use of locally available, recycled, and waste materials as growing media components have occurred in various regions of the world in extensive green roof technology. For eco-concept reasons, such a strategy appears to be appropriate, but can be problematic due to difficulties in obtaining proper parameters of growing substrate. The growing media should be properly engineered in order to enable the proper functioning of green roofs and provide suitable environment for ideal root growth. The aim of the study was to assess the utility of locally occurring waste materials for growing media composition and estimate plant- and time-dependent changes in the physico-chemical parameters of waste-based substrates in a simulated extensive green roof system during a two-year Sedum acre L. cultivation. Five different substrate compositions were prepared using silica waste, crushed brick, Ca- and Zn-aggregates, melaphyre, tuff, sand, muck soil, urban compost, spent mushroom, and coconut fibres. Optimal water capacity, particle-size distribution, pH and salts concentration were found in all substrates. A higher concentration of macronutrients (N, P, K, Mg) and trace elements (B, Cu, Fe, Mn, Zn, Cd, Ni, Pb, and Cr) was found in waste-based substrates than in the commercial medium. In comparison to the parameters determined before establish the experiment, bulk density of tested growing media decreased, except for the substrates where the source of organic matter was the rapidly mineralising spent mushroom. The organic matter content in substrates after the two-year vegetation increased in relation to the ready-made substrate, with the exception of the composition with spent mushroom. After two years of the experiment, all available macronutrients and trace elements (with the exception of mineral N, K, SO₄-S, and B) concentration were higher than in 2014, while pH, salt concentration was lower. In general, plants grown in waste substrates had lower dry matter content and higher biomass. A significantly higher biomass of S. acre L. was found in the first year of the experiment. In the second year of the research, the plants grown in the commercial medium, the substrate with silica waste, and the substrate with spent mushroom produced higher biomass than in the first year. No symptoms of abnormal growth were observed, despite the higher trace element concentrations in plants collected from waste-based substrate. Waste-based growing media can be considered as a valuable root environment for S. acre L. in an extensive green roof system.