This page has only limited features, please log in for full access.
Synchrotron X-ray µ- and nano-probes are increasingly affirming their relevance in cultural heritage applications, especially in material characterization of tiny and complex micro-samples which are typical from archaeological and artistic artifacts. For such purposes, synchrotron radiation facilities are tailoring and optimizing beamlines and set-ups for CH, taking also advantages from the challenges offered by the third-generation radiation sources. In ancient ceramics studies, relevant information for the identification of production centers and manufacture technology can be obtained in a non-invasive and non-destructive way at the micro-sample level by combining different SR based methods. However, the selection of appropriate beamlines, techniques and set-ups are critical for the success of the experiments. Fine and varnished wares (e.g., Attic and western-Greek colonial products) are an excellent case study for exploring challenges offered by synchrotron X-ray microprobes optimized to collect microchemical and phase-distribution maps. The determination of provenance and/or technological tracers is relevant in correctly classifying productions, often based only on ceramic paste, gloss macroscopic features or style. In addition, when these vessels are preserved in Museums as masterpieces or intact pieces the application of non-invasive approach at the micro sample is strictly required. Well-designed synchrotron µXRF and µXANES mapping experiments are able providing relevant clues for discriminating workshops and exploring technological aspects, which are fundamental in answering the current archaeological questions on varnished Greek or western-Greek colonial products.
Alessandra Gianoncelli; George Kourousias; Sebastian Schoeder; Antonella Santostefano; Maëva L’Héronde; Germana Barone; Paolo Mazzoleni; Simona Raneri. Synchrotron X-ray Microprobes: An Application on Ancient Ceramics. Applied Sciences 2021, 11, 8052 .
AMA StyleAlessandra Gianoncelli, George Kourousias, Sebastian Schoeder, Antonella Santostefano, Maëva L’Héronde, Germana Barone, Paolo Mazzoleni, Simona Raneri. Synchrotron X-ray Microprobes: An Application on Ancient Ceramics. Applied Sciences. 2021; 11 (17):8052.
Chicago/Turabian StyleAlessandra Gianoncelli; George Kourousias; Sebastian Schoeder; Antonella Santostefano; Maëva L’Héronde; Germana Barone; Paolo Mazzoleni; Simona Raneri. 2021. "Synchrotron X-ray Microprobes: An Application on Ancient Ceramics." Applied Sciences 11, no. 17: 8052.
The high resolution of synchrotron cryo-nano tomography can be easily undermined by setup instabilities and sample stage deficiencies such as runout or backlash. At the cost of limiting the sample visibility, especially in the case of bio-specimens, high contrast nano-beads are often added to the solution to provide a set of landmarks for a manual alignment. However, the spatial distribution of these reference points within the sample is difficult to control, resulting in many datasets without a sufficient amount of such critical features for tracking. Fast automatic methods based on tomography consistency are thus desirable, especially for biological samples, where regular, high contrast features can be scarce. Current off-the-shelf implementations of such classes of algorithms are slow if used on a real-world high-resolution dataset. In this paper, we present a fast implementation of a consistency-based alignment algorithm especially tailored to a multi-GPU system. Our implementation is released as open-source.
Francesco Guzzi; George Kourousias; Alessandra Gianoncelli; Lorella Pascolo; Andrea Sorrentino; Fulvio Billè; Sergio Carrato. Improving a Rapid Alignment Method of Tomography Projections by a Parallel Approach. Applied Sciences 2021, 11, 7598 .
AMA StyleFrancesco Guzzi, George Kourousias, Alessandra Gianoncelli, Lorella Pascolo, Andrea Sorrentino, Fulvio Billè, Sergio Carrato. Improving a Rapid Alignment Method of Tomography Projections by a Parallel Approach. Applied Sciences. 2021; 11 (16):7598.
Chicago/Turabian StyleFrancesco Guzzi; George Kourousias; Alessandra Gianoncelli; Lorella Pascolo; Andrea Sorrentino; Fulvio Billè; Sergio Carrato. 2021. "Improving a Rapid Alignment Method of Tomography Projections by a Parallel Approach." Applied Sciences 11, no. 16: 7598.
Progress in nanotechnology calls for material probing techniques of high sensitivity and resolution. Such techniques are also used for high-impact studies of nanoscale materials in medicine and biology. Soft X-ray microscopy has been successfully used for investigating complex biological processes occurring at micrometric and sub-micrometric length scales and is one of the most powerful tools in medicine and the life sciences. Here, we present the capabilities of the TwinMic soft X-ray microscopy end-station at the Elettra synchrotron in the context of medical and biological imaging, while we also describe novel uses and developments.
Alessandra Gianoncelli; Valentina Bonanni; Gianluca Gariani; Francesco Guzzi; Lorella Pascolo; Roberto Borghes; Fulvio Billè; George Kourousias. Soft X-ray Microscopy Techniques for Medical and Biological Imaging at TwinMic—Elettra. Applied Sciences 2021, 11, 7216 .
AMA StyleAlessandra Gianoncelli, Valentina Bonanni, Gianluca Gariani, Francesco Guzzi, Lorella Pascolo, Roberto Borghes, Fulvio Billè, George Kourousias. Soft X-ray Microscopy Techniques for Medical and Biological Imaging at TwinMic—Elettra. Applied Sciences. 2021; 11 (16):7216.
Chicago/Turabian StyleAlessandra Gianoncelli; Valentina Bonanni; Gianluca Gariani; Francesco Guzzi; Lorella Pascolo; Roberto Borghes; Fulvio Billè; George Kourousias. 2021. "Soft X-ray Microscopy Techniques for Medical and Biological Imaging at TwinMic—Elettra." Applied Sciences 11, no. 16: 7216.
Soft X-ray microscopy combined with X-ray Fluorescence is a powerful tool for analysing biological samples but it can be time consuming. Intelligent methods like Compressive Sensing allow for faster acquisitions by excluding areas of lesser interest.
George Kourousias; Fulvio Billè; Roberto Borghes; Lorella Pascolo; Alessandra Gianoncelli. Megapixel scanning transmission soft X-ray microscopy imaging coupled with compressive sensing X-ray fluorescence for fast investigation of large biological tissues. The Analyst 2021, 1 .
AMA StyleGeorge Kourousias, Fulvio Billè, Roberto Borghes, Lorella Pascolo, Alessandra Gianoncelli. Megapixel scanning transmission soft X-ray microscopy imaging coupled with compressive sensing X-ray fluorescence for fast investigation of large biological tissues. The Analyst. 2021; ():1.
Chicago/Turabian StyleGeorge Kourousias; Fulvio Billè; Roberto Borghes; Lorella Pascolo; Alessandra Gianoncelli. 2021. "Megapixel scanning transmission soft X-ray microscopy imaging coupled with compressive sensing X-ray fluorescence for fast investigation of large biological tissues." The Analyst , no. : 1.
The aim of this study was the detection and quantification of the Na+ depositions in the extracellular matrix of myocardial tissue, which are suggested to be bound by negatively charged glycosaminoglycan (GAG) structures. The presented experimental results are based on high resolution X-ray fluorescence (XRF) spectromicroscopy technique used to perform a comparative analysis of sodium containment in intracellular and interstitial spaces of cardiac tissues taken from animals selected by low and high sodium intake rates. The experimental results obtained show that high sodium daily intake can result in a remarkable increase of sodium concentration in the myocardial interstitium.
Igor Artyukov; Gregory Arutyunov; Maxim A. Bobrov; Inna Bukreeva; Alessia Cedola; Dmitrii Dragunov; Ruslan Feshchenko; Michela Fratini; Vadim M. Mitrokhin; Anna V. Sokolova; Alexander Vinogradov; Alessandra Gianoncelli. The first observation of osmotically neutral sodium accumulation in the myocardial interstitium. 2021, 1 .
AMA StyleIgor Artyukov, Gregory Arutyunov, Maxim A. Bobrov, Inna Bukreeva, Alessia Cedola, Dmitrii Dragunov, Ruslan Feshchenko, Michela Fratini, Vadim M. Mitrokhin, Anna V. Sokolova, Alexander Vinogradov, Alessandra Gianoncelli. The first observation of osmotically neutral sodium accumulation in the myocardial interstitium. . 2021; ():1.
Chicago/Turabian StyleIgor Artyukov; Gregory Arutyunov; Maxim A. Bobrov; Inna Bukreeva; Alessia Cedola; Dmitrii Dragunov; Ruslan Feshchenko; Michela Fratini; Vadim M. Mitrokhin; Anna V. Sokolova; Alexander Vinogradov; Alessandra Gianoncelli. 2021. "The first observation of osmotically neutral sodium accumulation in the myocardial interstitium." , no. : 1.
Filamentous cable bacteria display long-range electron transport, generating electrical currents over centimeter distances through a highly ordered network of fibers embedded in their cell envelope. The conductivity of these periplasmic wires is exceptionally high for a biological material, but their chemical structure and underlying electron transport mechanism remain unresolved. Here, we combine high-resolution microscopy, spectroscopy, and chemical imaging on individual cable bacterium filaments to demonstrate that the periplasmic wires consist of a conductive protein core surrounded by an insulating protein shell layer. The core proteins contain a sulfur-ligated nickel cofactor, and conductivity decreases when nickel is oxidized or selectively removed. The involvement of nickel as the active metal in biological conduction is remarkable, and suggests a hitherto unknown form of electron transport that enables efficient conduction in centimeter-long protein structures.
Henricus T. S. Boschker; Perran L. M. Cook; Lubos Polerecky; Raghavendran Thiruvallur Eachambadi; Helena Lozano; Silvia Hidalgo-Martinez; Dmitry Khalenkow; Valentina Spampinato; Nathalie Claes; Paromita Kundu; Da Wang; Sara Bals; Karina K. Sand; Francesca Cavezza; Tom Hauffman; Jesper Tataru Bjerg; Andre G. Skirtach; Kamila Kochan; Merrilyn McKee; Bayden Wood; Diana Bedolla; Alessandra Gianoncelli; Nicole M. J. Geerlings; Nani Van Gerven; Han Remaut; Jeanine S. Geelhoed; Ruben Millan-Solsona; Laura Fumagalli; Lars Peter Nielsen; Alexis Franquet; Jean V. Manca; Gabriel Gomila; Filip J. R. Meysman. Efficient long-range conduction in cable bacteria through nickel protein wires. Nature Communications 2021, 12, 1 -12.
AMA StyleHenricus T. S. Boschker, Perran L. M. Cook, Lubos Polerecky, Raghavendran Thiruvallur Eachambadi, Helena Lozano, Silvia Hidalgo-Martinez, Dmitry Khalenkow, Valentina Spampinato, Nathalie Claes, Paromita Kundu, Da Wang, Sara Bals, Karina K. Sand, Francesca Cavezza, Tom Hauffman, Jesper Tataru Bjerg, Andre G. Skirtach, Kamila Kochan, Merrilyn McKee, Bayden Wood, Diana Bedolla, Alessandra Gianoncelli, Nicole M. J. Geerlings, Nani Van Gerven, Han Remaut, Jeanine S. Geelhoed, Ruben Millan-Solsona, Laura Fumagalli, Lars Peter Nielsen, Alexis Franquet, Jean V. Manca, Gabriel Gomila, Filip J. R. Meysman. Efficient long-range conduction in cable bacteria through nickel protein wires. Nature Communications. 2021; 12 (1):1-12.
Chicago/Turabian StyleHenricus T. S. Boschker; Perran L. M. Cook; Lubos Polerecky; Raghavendran Thiruvallur Eachambadi; Helena Lozano; Silvia Hidalgo-Martinez; Dmitry Khalenkow; Valentina Spampinato; Nathalie Claes; Paromita Kundu; Da Wang; Sara Bals; Karina K. Sand; Francesca Cavezza; Tom Hauffman; Jesper Tataru Bjerg; Andre G. Skirtach; Kamila Kochan; Merrilyn McKee; Bayden Wood; Diana Bedolla; Alessandra Gianoncelli; Nicole M. J. Geerlings; Nani Van Gerven; Han Remaut; Jeanine S. Geelhoed; Ruben Millan-Solsona; Laura Fumagalli; Lars Peter Nielsen; Alexis Franquet; Jean V. Manca; Gabriel Gomila; Filip J. R. Meysman. 2021. "Efficient long-range conduction in cable bacteria through nickel protein wires." Nature Communications 12, no. 1: 1-12.
Hydrophobic treatment is one of the most important interventions usually carried out for the conservation of stone artefacts and monuments. The study here reported aims to answer a general question about how two polymers confer different protective performance. Two fluorinated-based polymer formulates applied on samples of Cusa’s stone confer a different level of water repellence and water vapour permeability. The observed protection action is here explained on the basis of chemico-physical interactions. The distribution of the polymer in the pore network was investigated using scanning electron microscopy and X-ray microscopy. The interactions between the stone substrate and the protective agents were investigated by means of solid state NMR spectroscopy. The ss-NMR findings reveal no significant changes in the chemical neighbourhood of the observed nuclei of each protective agent when applied onto the stone surface and provide information on the changes in the organization and dynamics of the studied systems, as well as on the mobility of polymer chains. This allowed us to explain the different macroscopic behaviours provided by each protective agent to the stone substrate.
Veronica Ciaramitaro; Alberto Spinella; Francesco Armetta; Roberto Scaffaro; Emmanuel Gulino; George Kourousias; Alessandra Gianoncelli; Eugenio Caponetti; Maria Saladino. A New Methodological Approach to Correlate Protective and Microscopic Properties by Soft X-ray Microscopy and Solid State NMR Spectroscopy: The Case of Cusa’s Stone. Applied Sciences 2021, 11, 5767 .
AMA StyleVeronica Ciaramitaro, Alberto Spinella, Francesco Armetta, Roberto Scaffaro, Emmanuel Gulino, George Kourousias, Alessandra Gianoncelli, Eugenio Caponetti, Maria Saladino. A New Methodological Approach to Correlate Protective and Microscopic Properties by Soft X-ray Microscopy and Solid State NMR Spectroscopy: The Case of Cusa’s Stone. Applied Sciences. 2021; 11 (13):5767.
Chicago/Turabian StyleVeronica Ciaramitaro; Alberto Spinella; Francesco Armetta; Roberto Scaffaro; Emmanuel Gulino; George Kourousias; Alessandra Gianoncelli; Eugenio Caponetti; Maria Saladino. 2021. "A New Methodological Approach to Correlate Protective and Microscopic Properties by Soft X-ray Microscopy and Solid State NMR Spectroscopy: The Case of Cusa’s Stone." Applied Sciences 11, no. 13: 5767.
Magnesium is an essential nutrient involved in many important processes in living organisms, including protein synthesis, cellular energy production and storage, cell growth and nucleic acid synthesis. In this study, we analysed the effect of magnesium deficiency on the proliferation of SaOS-2 osteosarcoma cells. When quiescent magnesium-starved cells were induced to proliferate by serum addition, the magnesium content was 2–3 times lower in cells maintained in a medium without magnesium compared with cells growing in the presence of the ion. Magnesium depletion inhibited cell cycle progression and caused the inhibition of cell proliferation, which was associated with mTOR hypophosphorylation at Serine 2448. In order to map the intracellular magnesium distribution, an analytical approach using synchrotron-based X-ray techniques was applied. When cell growth was stimulated, magnesium was mainly localized near the plasma membrane in cells maintained in a medium without magnesium. In non-proliferating cells growing in the presence of the ion, high concentration areas inside the cell were observed. These results support the role of magnesium in the control of cell proliferation, suggesting that mTOR may represent an important target for the antiproliferative effect of magnesium. Selective control of magnesium availability could be a useful strategy for inhibiting osteosarcoma cell growth.
Concettina Cappadone; Emil Malucelli; Maddalena Zini; Giovanna Farruggia; Giovanna Picone; Alessandra Gianoncelli; Andrea Notargiacomo; Michela Fratini; Carla Pignatti; Stefano Iotti; Claudio Stefanelli. Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells and Its Role in Proliferation. Nutrients 2021, 13, 1376 .
AMA StyleConcettina Cappadone, Emil Malucelli, Maddalena Zini, Giovanna Farruggia, Giovanna Picone, Alessandra Gianoncelli, Andrea Notargiacomo, Michela Fratini, Carla Pignatti, Stefano Iotti, Claudio Stefanelli. Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells and Its Role in Proliferation. Nutrients. 2021; 13 (4):1376.
Chicago/Turabian StyleConcettina Cappadone; Emil Malucelli; Maddalena Zini; Giovanna Farruggia; Giovanna Picone; Alessandra Gianoncelli; Andrea Notargiacomo; Michela Fratini; Carla Pignatti; Stefano Iotti; Claudio Stefanelli. 2021. "Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells and Its Role in Proliferation." Nutrients 13, no. 4: 1376.
Background: Increasing evidences support a correlation between magnesium (Mg) homeostasis and colorectal cancer (CRC). Nevertheless, the role of Mg and its transporters as diagnostic markers in CRC is still a matter of debate. In this study we combined X-ray Fluorescence Microscopy and databases information to investigate the possible correlation between Mg imbalance and CRC. Methods: CRC tissue samples and their non-tumoural counterpart from four patients were collected and analysed for total Mg level and distribution by X-Ray Fluorescence Microscopy. We also reviewed the scientific literature and the main tissue expression databases to collect data on Mg transporters expression in CRC. Results: We found a significantly higher content of total Mg in CRC samples when compared to non-tumoural tissues. Mg distribution was also impaired in CRC. Conversely, we evidenced an uncertain correlation between Mg transporters expression and colon malignancies. Discussion: Although further studies are necessary to determine the correlation between different cancer types and stages, this is the first report proposing the measurement of Mg tissue localisation as a marker in CRC. This study represents thus a proof-of-concept that paves the way for the design of a larger prospective investigation of Mg in CRC.
Davide Schiroli; Chiara Marraccini; Eleonora Zanetti; Moira Ragazzi; Alessandra Gianoncelli; Eleonora Quartieri; Elisa Gasparini; Stefano Iotti; Roberto Baricchi; Lucia Merolle. Imbalance of Mg Homeostasis as a Potential Biomarker in Colon Cancer. Diagnostics 2021, 11, 727 .
AMA StyleDavide Schiroli, Chiara Marraccini, Eleonora Zanetti, Moira Ragazzi, Alessandra Gianoncelli, Eleonora Quartieri, Elisa Gasparini, Stefano Iotti, Roberto Baricchi, Lucia Merolle. Imbalance of Mg Homeostasis as a Potential Biomarker in Colon Cancer. Diagnostics. 2021; 11 (4):727.
Chicago/Turabian StyleDavide Schiroli; Chiara Marraccini; Eleonora Zanetti; Moira Ragazzi; Alessandra Gianoncelli; Eleonora Quartieri; Elisa Gasparini; Stefano Iotti; Roberto Baricchi; Lucia Merolle. 2021. "Imbalance of Mg Homeostasis as a Potential Biomarker in Colon Cancer." Diagnostics 11, no. 4: 727.
Radiation damage upon soft X-ray exposure is an important issue to be considered in soft X-ray microscopy. The work presented here is part of a more extended study on the topic and focuses on the effects of soft X-rays on paraffin, a common embedding medium for soft-tissues, and on ultralene and Si3N4 windows as sample supports. Our studies suggest that the sample environment indeed plays an important role in the radiation damage process and therefore should be carefully taken into account for the analysis and interpretation of new data. The radiation damage effects were followed over time using a combination of Fourier transform infrared (FTIR) microspectroscopy and X-ray fluorescence (XRF), and it was demonstrated that, for higher doses, an oxidation of both embedding medium and ultralene substrate takes place after the irradiated sample is exposed to air. This oxidation is reflected in a clear increase of C=O and O—H infrared bands and on the XRF oxygen maps, correlated with a decrease of the aliphatic infrared signal. The results also show that the oxidation process may affect quantitative evaluation of light element concentrations.
Diana E. Bedolla; Giovanni Birarda; Sabina Giannotta; Valentina Faoro; Alberto Cescato; Lisa Vaccari; Alessandra Gianoncelli. Oxidation of ultralene and paraffin due to radiation damage after exposure to soft X-rays probed by FTIR microspectroscopy and X-ray fluorescence. Journal of Synchrotron Radiation 2021, 28, 231 -239.
AMA StyleDiana E. Bedolla, Giovanni Birarda, Sabina Giannotta, Valentina Faoro, Alberto Cescato, Lisa Vaccari, Alessandra Gianoncelli. Oxidation of ultralene and paraffin due to radiation damage after exposure to soft X-rays probed by FTIR microspectroscopy and X-ray fluorescence. Journal of Synchrotron Radiation. 2021; 28 (1):231-239.
Chicago/Turabian StyleDiana E. Bedolla; Giovanni Birarda; Sabina Giannotta; Valentina Faoro; Alberto Cescato; Lisa Vaccari; Alessandra Gianoncelli. 2021. "Oxidation of ultralene and paraffin due to radiation damage after exposure to soft X-rays probed by FTIR microspectroscopy and X-ray fluorescence." Journal of Synchrotron Radiation 28, no. 1: 231-239.
The severity of osteoporosis in humans manifests in its high incidence and by its complications that diminish quality of life. A societal consequence of osteoporosis is the substantial burden that it inflicts upon patients and their families. Several bone-modifying drugs have been prescribed to patients with osteoporosis. However, evidence for their anti-fracture efficacy remains inconclusive. To the contrary, long-term use of anti-osteoporotic drugs such as bisphosphonates and Denosumab, an RANKL inhibitor, have resulted in adverse events. We now present an alternative and adjuvant approach for treatment of osteoporosis. The data derive from in vivo studies in an ovariectomized rat model and from a randomized double blind, placebo-controlled human clinical study. Both studies involved treatment with Panaceo Micro Activation (PMA)-zeolite-clinoptilolite, a defined cation exchange clinoptilolite, which clearly improved all bone histomorphometric parameters examined from ovariectomized animals, indicative for increased bone formation. Moreover, intervention with PMA-zeolite-clinoptilolite for one year proved safe in humans. Furthermore, patients treated with PMA-zeolite-clinoptilolite showed an increase in bone mineral density, an elevated level of markers indicative of bone formation, a significant reduction in pain, and significantly improved quality of life compared with patients in the control (placebo) group. These encouraging positive effects of PMA-zeolite-clinoptilolite on bone integrity and on osteoporosis warrant further evaluation of treatment with PMA-zeolite-clinoptilolite as a new alternative adjuvant therapy for osteoporosis.
Sandra Kraljević Pavelić; Vedran Micek; Dragica Bobinac; Edo Bazdulj; Alessandra Gianoncelli; Dalibor Krpan; Marta Žuvić; Sandra Eisenwagen; Peter J Stambrook; Krešimir Pavelić. Treatment of osteoporosis with a modified zeolite shows beneficial effects in an osteoporotic rat model and a human clinical trial. Experimental Biology and Medicine 2020, 246, 529 -537.
AMA StyleSandra Kraljević Pavelić, Vedran Micek, Dragica Bobinac, Edo Bazdulj, Alessandra Gianoncelli, Dalibor Krpan, Marta Žuvić, Sandra Eisenwagen, Peter J Stambrook, Krešimir Pavelić. Treatment of osteoporosis with a modified zeolite shows beneficial effects in an osteoporotic rat model and a human clinical trial. Experimental Biology and Medicine. 2020; 246 (5):529-537.
Chicago/Turabian StyleSandra Kraljević Pavelić; Vedran Micek; Dragica Bobinac; Edo Bazdulj; Alessandra Gianoncelli; Dalibor Krpan; Marta Žuvić; Sandra Eisenwagen; Peter J Stambrook; Krešimir Pavelić. 2020. "Treatment of osteoporosis with a modified zeolite shows beneficial effects in an osteoporotic rat model and a human clinical trial." Experimental Biology and Medicine 246, no. 5: 529-537.
In order to push the spatial resolution limits to the nanoscale, synchrotron-based soft X-ray microscopy (XRM) experiments require higher radiation doses to be delivered to materials. Nevertheless, the associated radiation damage impacts on the integrity of delicate biological samples. Herein, the extent of soft X-ray radiation damage in popular thin freeze-dried brain tissue samples mounted onto Si3N4 membranes, as highlighted by Fourier transform infrared microscopy (FTIR), is reported. The freeze-dried tissue samples were found to be affected by general degradation of the vibrational architecture, though these effects were weaker than those observed in paraffin-embedded and hydrated systems reported in the literature. In addition, weak, reversible and specific features of the tissue–Si3N4 interaction could be identified for the first time upon routine soft X-ray exposures, further highlighting the complex interplay between the biological sample, its preparation protocol and X-ray probe.
Artur D. Surowka; A. Gianoncelli; G. Birarda; S. Sala; N. Cefarin; A. Matruglio; M. Szczerbowska-Boruchowska; A. Ziomber-Lisiak; L. Vaccari. Soft X-ray induced radiation damage in thin freeze-dried brain samples studied by FTIR microscopy. Journal of Synchrotron Radiation 2020, 27, 1 .
AMA StyleArtur D. Surowka, A. Gianoncelli, G. Birarda, S. Sala, N. Cefarin, A. Matruglio, M. Szczerbowska-Boruchowska, A. Ziomber-Lisiak, L. Vaccari. Soft X-ray induced radiation damage in thin freeze-dried brain samples studied by FTIR microscopy. Journal of Synchrotron Radiation. 2020; 27 (5):1.
Chicago/Turabian StyleArtur D. Surowka; A. Gianoncelli; G. Birarda; S. Sala; N. Cefarin; A. Matruglio; M. Szczerbowska-Boruchowska; A. Ziomber-Lisiak; L. Vaccari. 2020. "Soft X-ray induced radiation damage in thin freeze-dried brain samples studied by FTIR microscopy." Journal of Synchrotron Radiation 27, no. 5: 1.
Low energy X-ray fluorescence reveals the effects of photobiomodulation therapy on sperm.
Luisa Zupin; Lorella Pascolo; Alessandra Gianoncelli; Gianluca Gariani; Stefania Luppi; Elena Giolo; Giulia Ottaviani; Sergio Crovella; Giuseppe Ricci. Synchrotron radiation soft X-ray microscopy and low energy X-ray fluorescence to reveal elemental changes in spermatozoa treated with photobiomodulation therapy. Analytical Methods 2020, 12, 3691 -3696.
AMA StyleLuisa Zupin, Lorella Pascolo, Alessandra Gianoncelli, Gianluca Gariani, Stefania Luppi, Elena Giolo, Giulia Ottaviani, Sergio Crovella, Giuseppe Ricci. Synchrotron radiation soft X-ray microscopy and low energy X-ray fluorescence to reveal elemental changes in spermatozoa treated with photobiomodulation therapy. Analytical Methods. 2020; 12 (29):3691-3696.
Chicago/Turabian StyleLuisa Zupin; Lorella Pascolo; Alessandra Gianoncelli; Gianluca Gariani; Stefania Luppi; Elena Giolo; Giulia Ottaviani; Sergio Crovella; Giuseppe Ricci. 2020. "Synchrotron radiation soft X-ray microscopy and low energy X-ray fluorescence to reveal elemental changes in spermatozoa treated with photobiomodulation therapy." Analytical Methods 12, no. 29: 3691-3696.
In this study, we explore the behaviour of intracellular magnesium during bone phenotype modulation in a 3D cell model built to mimic osteogenesis. In addition, we measured the amount of magnesium in the mineral depositions generated during osteogenic induction. A two-fold increase of intracellular magnesium content was found, both at three and seven days from the induction of differentiation. By X-ray microscopy, we characterized the morphology and chemical composition of the mineral depositions secreted by 3D cultured differentiated cells finding a marked co-localization of Mg with P at seven days of differentiation. This is the first experimental evidence on the presence of Mg in the mineral depositions generated during biomineralization, suggesting that Mg incorporation occurs during the bone forming process. In conclusion, this study on the one hand attests to an evident involvement of Mg in the process of cell differentiation, and, on the other hand, indicates that its multifaceted role needs further investigation.
Giovanna Picone; Concettina Cappadone; Alice Pasini; Joseph Lovecchio; Marilisa Cortesi; Giovanna Farruggia; Marco Lombardo; Alessandra Gianoncelli; Lucia Mancini; Menk Ralf H.; Sandro Donato; Emanuele Giordano; Emil Malucelli; Stefano Iotti. Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells. International Journal of Molecular Sciences 2020, 21, 2368 .
AMA StyleGiovanna Picone, Concettina Cappadone, Alice Pasini, Joseph Lovecchio, Marilisa Cortesi, Giovanna Farruggia, Marco Lombardo, Alessandra Gianoncelli, Lucia Mancini, Menk Ralf H., Sandro Donato, Emanuele Giordano, Emil Malucelli, Stefano Iotti. Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells. International Journal of Molecular Sciences. 2020; 21 (7):2368.
Chicago/Turabian StyleGiovanna Picone; Concettina Cappadone; Alice Pasini; Joseph Lovecchio; Marilisa Cortesi; Giovanna Farruggia; Marco Lombardo; Alessandra Gianoncelli; Lucia Mancini; Menk Ralf H.; Sandro Donato; Emanuele Giordano; Emil Malucelli; Stefano Iotti. 2020. "Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells." International Journal of Molecular Sciences 21, no. 7: 2368.
Environmental contamination due to human activities is a worldwide problem that has led to the development of different remediation techniques, including biotechnological approaches such as phytoextraction and phytostabilization. These techniques take advantage of pioneer plants that naturally develop tolerance mechanisms to survive in extreme environments. A multi-technique and multi-disciplinary approach was applied for the investigation of Helichrysum microphyllum subsp. tyrrhenicum samples, bulk soil, and rhizospheres collected from a metal-extreme environment (Zn-Pb mine of Campo Pisano, SW Sardinia, Italy). Zinc, Pb, and Cd are the most abundant metals, with Zn attaining 3 w/w% in the rhizosphere solid materials, inducing oxidative stress in the roots as revealed by infrared microspectroscopy (IR). X-ray diffraction (XRD), scanning electron microscopy (SEM), and chemical analysis coupled with synchrotron radiation-based (SR) techniques demonstrate that quartz, dolomite, and weddellite biominerals precipitate in roots, stems, and leaves, likely as a response to environmental stress. In the rhizosphere, Zn chemical speciation is mainly related to the Zn ore minerals (smithsonite and hydrozincite) whereas, in plant tissues, Zn is primarily bound to organic compounds such as malate, cysteine, and histidine molecules that act as metal binders and, eventually, detoxification agents for the Zn excess. These findings suggest that H. microphyllum subsp. tyrrhenicum has developed its own adaptation strategy to survive in polluted substrates, making it a potential candidate for phytostabilization aimed at mitigating the dispersion of metals in the surrounding areas.
Maria Enrica Boi; Daniela Medas; Giuliana Aquilanti; Gianluigi Bacchetta; Giovanni Birarda; Giovanna Cappai; Ilaria Carlomagno; Maria Antonietta Casu; Alessandra Gianoncelli; Carlo Meneghini; Martina Piredda; Francesca Podda; Marco Porceddu; Valentina Rimondi; Lisa Vaccari; Giovanni De Giudici. Mineralogy and Zn Chemical Speciation in a Soil-Plant System from a Metal-Extreme Environment: A Study on Helichrysum microphyllum subsp. tyrrhenicum (Campo Pisano Mine, SW Sardinia, Italy). Minerals 2020, 10, 259 .
AMA StyleMaria Enrica Boi, Daniela Medas, Giuliana Aquilanti, Gianluigi Bacchetta, Giovanni Birarda, Giovanna Cappai, Ilaria Carlomagno, Maria Antonietta Casu, Alessandra Gianoncelli, Carlo Meneghini, Martina Piredda, Francesca Podda, Marco Porceddu, Valentina Rimondi, Lisa Vaccari, Giovanni De Giudici. Mineralogy and Zn Chemical Speciation in a Soil-Plant System from a Metal-Extreme Environment: A Study on Helichrysum microphyllum subsp. tyrrhenicum (Campo Pisano Mine, SW Sardinia, Italy). Minerals. 2020; 10 (3):259.
Chicago/Turabian StyleMaria Enrica Boi; Daniela Medas; Giuliana Aquilanti; Gianluigi Bacchetta; Giovanni Birarda; Giovanna Cappai; Ilaria Carlomagno; Maria Antonietta Casu; Alessandra Gianoncelli; Carlo Meneghini; Martina Piredda; Francesca Podda; Marco Porceddu; Valentina Rimondi; Lisa Vaccari; Giovanni De Giudici. 2020. "Mineralogy and Zn Chemical Speciation in a Soil-Plant System from a Metal-Extreme Environment: A Study on Helichrysum microphyllum subsp. tyrrhenicum (Campo Pisano Mine, SW Sardinia, Italy)." Minerals 10, no. 3: 259.
The use of quantum dots (QDs) is widespread and as such, the potential risk associated with their dispersion in the environment has stimulated research on their interaction with potential sensitive receptors.
Marta Marmiroli; Giovanni Orazio Lepore; Luca Pagano; Francesco D'Acapito; Alessandra Gianoncelli; Marco Villani; Laura Lazzarini; Jason C. White; Nelson Marmiroli. The fate of CdS quantum dots in plants as revealed by extended X-ray absorption fine structure (EXAFS) analysis. Environmental Science: Nano 2020, 7, 1150 -1162.
AMA StyleMarta Marmiroli, Giovanni Orazio Lepore, Luca Pagano, Francesco D'Acapito, Alessandra Gianoncelli, Marco Villani, Laura Lazzarini, Jason C. White, Nelson Marmiroli. The fate of CdS quantum dots in plants as revealed by extended X-ray absorption fine structure (EXAFS) analysis. Environmental Science: Nano. 2020; 7 (4):1150-1162.
Chicago/Turabian StyleMarta Marmiroli; Giovanni Orazio Lepore; Luca Pagano; Francesco D'Acapito; Alessandra Gianoncelli; Marco Villani; Laura Lazzarini; Jason C. White; Nelson Marmiroli. 2020. "The fate of CdS quantum dots in plants as revealed by extended X-ray absorption fine structure (EXAFS) analysis." Environmental Science: Nano 7, no. 4: 1150-1162.
Carbon nanotubes (CNTs) have been promoted as nanodevices in the biomedicine areas of diagnosis and therapy. In terms of drug delivery, CNTs could be useful in the case of hydrophobic drugs and multiple co-deliveries of therapeutic and imaging molecules. Epithelial cells, being specialized in absorption and secretion, are the first barrier to overcome. At the same time, several frequent tumors are of epithelial origin (cervix, ovarian, liver). Synchrotron-based imaging and spectroscopic techniques are suitable tools to follow the fate of nanodrugs in epithelial cells. We present an example of multi-microscopy approach combining soft X-ray spectromicroscopy at the TwinMic beamline (Elettra Synchrotron, Trieste, Italy) with light and Scanning Electron (SEM) microscopies. In particular, we show that 2–4 h exposure to SWCNTs is not toxic (no viability changes) to a model of cervical epithelial cells, even though it causes temporary perturbation in the membrane and intracellular structures of exposed cells.
A. Gianoncelli; R. Delfino; S. Sala; G. Kourousias; S. Giordani; F. Romano; G. Ricci; L. Pascolo. Synchrotron soft X-ray microscopy and XRF to image Single-walled carbon nanotubes in epithelial cells. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 2020, 465, 79 -84.
AMA StyleA. Gianoncelli, R. Delfino, S. Sala, G. Kourousias, S. Giordani, F. Romano, G. Ricci, L. Pascolo. Synchrotron soft X-ray microscopy and XRF to image Single-walled carbon nanotubes in epithelial cells. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 2020; 465 ():79-84.
Chicago/Turabian StyleA. Gianoncelli; R. Delfino; S. Sala; G. Kourousias; S. Giordani; F. Romano; G. Ricci; L. Pascolo. 2020. "Synchrotron soft X-ray microscopy and XRF to image Single-walled carbon nanotubes in epithelial cells." Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 465, no. : 79-84.
Lorella Pascolo; Luisa Zupin; Alessandra Gianoncelli; Elena Giolo; Stefania Luppi; Monica Martinelli; Daniela De Rocco; Simone Sala; Sergio Crovella; Giuseppe Ricci. XRF analyses reveal that capacitation procedures produce changes in magnesium and copper levels in human sperm. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 2019, 459, 120 -124.
AMA StyleLorella Pascolo, Luisa Zupin, Alessandra Gianoncelli, Elena Giolo, Stefania Luppi, Monica Martinelli, Daniela De Rocco, Simone Sala, Sergio Crovella, Giuseppe Ricci. XRF analyses reveal that capacitation procedures produce changes in magnesium and copper levels in human sperm. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 2019; 459 ():120-124.
Chicago/Turabian StyleLorella Pascolo; Luisa Zupin; Alessandra Gianoncelli; Elena Giolo; Stefania Luppi; Monica Martinelli; Daniela De Rocco; Simone Sala; Sergio Crovella; Giuseppe Ricci. 2019. "XRF analyses reveal that capacitation procedures produce changes in magnesium and copper levels in human sperm." Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 459, no. : 120-124.
Laboratory and synchrotron X-ray tomography are powerful tools for non-invasive studies of biological samples at micrometric resolution. In particular, the development of phase contrast imaging is enabling the visualization of sample details with a small range of attenuation coefficients, thus allowing in-depth analyses of anatomical and histological structures. Reproductive medicine is starting to profit from these techniques, mainly applied to animal models. This study reports the first imaging of human ovarian tissue where the samples consisted of surgically obtained millimetre fragments, properly fixed, stained with osmium tetroxide and included in epoxydic resin. Samples were imaged by the use of propagation phase contrast synchrotron radiation micro-computed tomography (microCT), obtained at the SYRMEP beamline of Elettra light source (Trieste, Italy), and X-ray absorption microCT at the Theoretical Biology MicroCT Imaging Laboratory in Vienna, Austria. The reconstructed microCT images were compared with the soft X-ray absorption and phase contrast images acquired at the TwinMic beamline of Elettra in order to help with the identification of structures. The resulting images allow the regions of the cortex and medulla of the ovary to be distinguished, identifying early-stage follicles and visualizing the distribution of blood vessels. The study opens to further application of micro-resolved 3D imaging to improve the understanding of human ovary's structure and support diagnostics as well as advances in reproductive technologies.
Lorella Pascolo; Gabriela Sena; Alessandra Gianoncelli; Alice Cernogoraz; Geroge Kourousias; Brian D. Metscher; Federico Romano; Gabriella Zito; Serena Pacilè; Regina Barroso; Giuliana Tromba; Marina Zweyer; Giuseppe Ricci. Hard and soft X-ray imaging to resolve human ovarian cortical structures. Journal of Synchrotron Radiation 2019, 26, 1322 -1329.
AMA StyleLorella Pascolo, Gabriela Sena, Alessandra Gianoncelli, Alice Cernogoraz, Geroge Kourousias, Brian D. Metscher, Federico Romano, Gabriella Zito, Serena Pacilè, Regina Barroso, Giuliana Tromba, Marina Zweyer, Giuseppe Ricci. Hard and soft X-ray imaging to resolve human ovarian cortical structures. Journal of Synchrotron Radiation. 2019; 26 (4):1322-1329.
Chicago/Turabian StyleLorella Pascolo; Gabriela Sena; Alessandra Gianoncelli; Alice Cernogoraz; Geroge Kourousias; Brian D. Metscher; Federico Romano; Gabriella Zito; Serena Pacilè; Regina Barroso; Giuliana Tromba; Marina Zweyer; Giuseppe Ricci. 2019. "Hard and soft X-ray imaging to resolve human ovarian cortical structures." Journal of Synchrotron Radiation 26, no. 4: 1322-1329.
Juncus acutus, an halophite plant pioneer in extremely polluted mine areas, was harvested in three different locations of Sardinia (Italy), having Zn soil concentration up to 80g/kg, and Zn water concentration ranging between 10(-3)g/L and 10(-1)g/L. Rhizosphere and plant samples were investigated combining X-ray microscopy (XM)/spectroscopy (XAFS) and infrared microspectroscopy (FTIR) to elucidate the chemical composition, (bio)mineralogy and Zn coordinative environment. The multi-technique approach allowed recognizing different biomineralization processes, and Zn complexes in the plant tissues. The Zn chemical environment in root biominerals is multi-phase and, depending on the sampling site, can comprise amorphous Zn silicate, Zn apatite, hydrozincite, and Zn sulphate. Zn cysteine and Zn histidine, complexes quoted as part of a detoxification strategy, were found mainly in plants from the site where the Zn water concentration has the highest values. This different site-specific mode of Zn biomineralization has relevant implications for phytoremediation techniques and for further biotechnology development, which can be better designed and developed after knowledge of site-specific-molecular processes ruling mineral evolution and biomineralization. Carboxylic groups and organic compounds (lignin, cellulose, hemicellulose, pectin and esters) were identified by FTIR analysis, thought the Zn speciation is not apparently linked to these carboxylic group rich biopolymers.
Daniela Medas; Giovanni De Giudici; Claudia Pusceddu; Maria Antonietta Casu; Giovanni Birarda; Lisa Vaccari; Alessandra Gianoncelli; Carlo Meneghini. Impact of Zn excess on biomineralization processes in Juncus acutus grown in mine polluted sites. Journal of Hazardous Materials 2019, 370, 98 -107.
AMA StyleDaniela Medas, Giovanni De Giudici, Claudia Pusceddu, Maria Antonietta Casu, Giovanni Birarda, Lisa Vaccari, Alessandra Gianoncelli, Carlo Meneghini. Impact of Zn excess on biomineralization processes in Juncus acutus grown in mine polluted sites. Journal of Hazardous Materials. 2019; 370 ():98-107.
Chicago/Turabian StyleDaniela Medas; Giovanni De Giudici; Claudia Pusceddu; Maria Antonietta Casu; Giovanni Birarda; Lisa Vaccari; Alessandra Gianoncelli; Carlo Meneghini. 2019. "Impact of Zn excess on biomineralization processes in Juncus acutus grown in mine polluted sites." Journal of Hazardous Materials 370, no. : 98-107.