The current farming systems strongly depend on chemical fertilizers (CF), which are widely applied to increase crop yield worldwide. However, although CF enhance crop yield in the short term, their excessive and long-term application can have adverse effects on environmental and human health. One of the most important goals of sustainable agriculture is substituting CF with organic manures. Organic manures can be used as a low-cost and safe alternative for CF. They contain essential nutrients for crop growth, improve soil conditions and nutrient availability, increase plant growth, and ultimately enhance yield. The application of organic manures to medicinal plants (MP) is more critical than to other plants, because organic manures not only enhance the growth and productivity of MP but also modify quality of their products. In this review, the effect of different types of organic manures on the biomass, content and chemical compositions of essential oil and antioxidant activity of various MP has been investigated. The included information was gathered from scientific databases such as Science Direct, Google Scholar, PubMed, and Scopus. Many of the collected studies showed that organic manures increase biomass and improve the quality of these plants. The findings of this review indicate that broiler litter (BL) and compost (C) are highly recommended as organic manures to promote biomass. Moreover, C, sheep manure, and vermicompost (VC) are suggested as the optimal organic manures for enhancing the essential oil content. Organic manures significantly changed the aroma profile of the essential oils and in many cases, they enhanced major chemical compositions. The usage of VC raised the content of the linalool of studied MP. Most of the organic manures, especially BL, VC, farmyard manure, and poultry manure increased the antioxidant activity of these plants. Hence, the utilization of organic manures can be recommended for productivity enhancement and quality improvement of MP.

Understanding the potential uptake and biodistribution of engineered nanoparticles (ENPs) in soil-grown plants is imperative for realistic toxicity and risk assessment considering the oral intake of edibles by humans. Herein, growing N-fixing symbiont (Bradyrhizobium japonicum) inoculated soybean (Glycine max (L.) Merr.) for a full lifecycle of 120 days, we assessed the potential influence of particle size (25, 50, and 250 nm) and concentration (0, 50, 100, 200, and 500 mg/kg soil) of Copper oxide nanoparticles (CuONPs) on: (1) root system architecture, (2) soil physicochemical attributes at the soil–root interface, and (3) Cu transport and accumulation in root, stem, leaf, and seed in soybean, and compared them with the soluble Cu²⁺ ions and water-only controls. Finally, we performed a comparative assessment of total seed Cu levels in soybean with other valuable food sources for Cu intake and discussed potential human health implications. Results showed particle size- and concentration-dependent influence of CuONPs on Cu uptake and distribution in root, stem, leaf, and seed. Alterations in root architecture (root biomass, length, volume, and area) were dependent on the Cu compound types, Cu concentrations, and their interactions. Concentration–response relationships for all three sizes of CuONPs and Cu²⁺ ions were found to be linear. Furthermore, CuONPs and Cu²⁺ ions had inhibitory effects on root growth and development. Overall, soybean responses to the smallest size of CuONPs–25 nm—were greater for all parameters tested compared to the two larger-sized CuONPs (50 nm, 250 nm) or Cu²⁺ ions. Results suggest that minor changes in soil-root physicochemical attributes may not be a major driver for Cu uptake in soybean. Cu bioaccumulation followed the order: root > leaf > stem > seed. Despite reduction in root architecture and seed yield, the smallest size CuONPs–25 nm led to increased total seed Cu uptake compared to the larger-sized CuONPs or Cu²⁺ ions. Our findings also suggest that soil amendment with CuONPs, and more so with the smallest size of CuONPs–25 nm—could significantly improve seed nutritional Cu value in soybean as reflected by the % Daily Values (DV) and are rated “Good” to “Very Good” according to the “World’s Healthiest Foods” rating. However, until the potential toxicity and risk from CuONP-fortified soybean seed ingestion is characterized in humans, we caution recommending such seeds for daily human consumption when addressing food Cu-deficiency and associated diseases, globally.