As international food trade increases, consumers are becoming increasingly interested in food safety and authenticity, which are linked to geographical origin. Adzuki beans (Vigna angularis) are cultivated worldwide, but there are no tools for accurately discriminating their geographical origin. Thus, our study aims to develop a method for discriminating the geographical origin of adzuki beans through targeted and non-targeted metabolite profiling with gas chromatography time-of-flight mass spectrometry combined with multivariate analysis. Orthogonal partial least squares discriminant analysis showed clear discrimination between adzuki beans cultivated in Korea and China. Non-targeted metabolite profiling showed better separation than targeted profiling. Furthermore, citric acid and malic acid were the most notable metabolites for discriminating adzuki beans cultivated in Korea and China. The geographical discrimination method combining non-targeted metabolite profiling and pareto-scaling showed excellent predictability (Q² = 0.812). Therefore, it is a suitable prediction tool for the discrimination of geographical origin and is expected to be applicable to the geographical authentication of adzuki beans.

Theory predicts that sympatric predators compete for food under conditions of limited resources. Competition would occur even within the same species, between neighboring populations, because of overlapping foraging habits. Thus, neighboring populations of the same species are hypothesized to face strong competition. To test the hypothesis that intra-specific competition is more intense than inter-specific competition owing to a lack of niche partitioning, we estimated the foraging area and diving depths of two colonial seabird species at two neighboring colonies. Using GPS and time-depth recorders, we tracked foraging space use of sympatric breeding Chinstrap and Gentoo penguins at Ardley Island (AI) and Narębski Point (NP) at King George Island, Antarctica. GPS tracks showed that there was a larger overlap in the foraging areas between the two species than within each species. In dive parameters, Gentoo penguins performed deeper and longer dives than Chinstrap penguins at the same colonies. At the colony level, Gentoo penguins from NP undertook deeper and longer dives than those at AI, whereas Chinstrap penguins did not show such intra-specific differences in dives. Stable isotope analysis of δ¹³C and δ¹⁵N isotopes in blood demonstrated both inter- and intra-specific differences. Both species of penguin at AI exhibited higher δ¹³C and δ¹⁵N values than those at NP, and in both locations, Gentoo penguins had higher δ¹³C and lower δ¹⁵N values than Chinstrap penguins. Isotopic niches showed that there were lower inter-specific overlaps than intra-specific overlaps. This suggests that, despite the low intra-specific spatial overlap, diets of conspecifics from different colonies remained more similar, resulting in the higher isotopic niche overlaps. Collectively, our results support the hypothesis that intra-specific competition is higher than inter-specific competition, leading to spatial segregation of the neighboring populations of the same species.

Eresus kollari is typically found in dry grasslands and stony steppes; however, in South Korea, it has been primarily found in the humid environments of cemeteries. These spiders have highly localized habitats, and their burrowing and nest‐building habits make them difficult to find and observe in the field. This study investigated the cemetery habitat environment, which has not been well‐researched previously. Because direct observation without separating the spiders from their nests is difficult, the relationship between nest size and individual size was studied to estimate the size of the spiders based on their nests. The cemeteries are open and sunny, with loess‐like soil. The ground surface is mainly covered with Korean lawn grass, lacking trees or shrubs, and the herbaceous layer consists of low‐growing plants. The nests of E. kollari consist of an aboveground web canopy and an underground retreat, and they were found just below the grass. The height of the web canopy and the depth of the retreat were not correlated with the individuals' body size. However, the overall nest diameter exhibited a positive exponential relationship with both cephalothorax and abdomen size. In particular, the product of cephalothorax width and length was the most informative parameter for predicting nest diameter. Remains of beetles and ants were found in the web canopy, suggesting that these insects constitute the primary prey of E. kollari. However, in the case of very small ants, it seems that Plagiolepis manczshzurica may be a predator of E. kollari, as ants were found alongside E. kollari remains within the nest. In South Korea, E. kollari is not designated as an endangered species, but its habitat and population have reportedly been in decline. Notably, the habitat observed during the study period was destroyed by construction activities. Therefore, research and conservation measures for this species are necessary.

Colorectal cancer (CRC) is a significant global health issue characterized by a high prevalence of KRAS gene mutations. The RAS/MAPK pathway, involving KRAS, plays a crucial role in CRC progression. Although some RAS inhibitors have been approved, their efficacy in CRC is limited. To overcome these limitations, pan-RAF inhibitors targeting A-Raf, B-Raf, and C-Raf have emerged as promising therapeutic strategies. However, resistance to RAF inhibition and the presence of an immunosuppressive tumor microenvironment (TME) pose additional obstacles to effective therapy. Here, we evaluated the potential of a novel pan-RAF inhibitor, SJ-C1044, for targeting mutant KRAS-mediated signaling and inhibiting CRC cell proliferation. Notably, SJ-C1044 also exhibited inhibitory effects on immunokinases, specifically, CSF1R, VEGFR2, and TIE2, which play crucial roles in immune suppression. SJ-C1044 demonstrated potent antitumor activity in xenograft models of CRC harboring KRAS or BRAF mutations. Importantly, treatment with SJ-C1044 resulted in increased infiltration of T cells and reduced presence of tumor-associated macrophages and regulatory T cells within the TME. Thus, SJ-C1044 shows immunomodulatory potential and the ability to enhance antitumor responses. The study underscores the therapeutic potential of SJ-C1044 as a novel pan-RAF inhibitor capable of targeting oncogenic signaling pathways and overcoming immune suppression in CRC.

The genus Diduga Moore, [1887] is reviewed with 11 species from Laos, including one new species, D. bantha sp. nov. Illustrations of adults and genitalia of all examined species from Laos are provided, with the checklist of Laotian species of the genus Diduga.

The Hedgehog (Hh) signaling pathway plays an essential role in the initiation and progression of prostate cancer. This is mediated by transcriptional factors belonging to the GLI (glioma-associated oncogene) family, which regulate downstream targets to drive prostate cancer progression. The activity of GLI proteins is tightly controlled by a range of mechanisms, including molecular interactions and post-translational modifications. In particular, mitogenic and oncogenic signaling pathways have been shown to regulate GLI protein activity independently of upstream Hh pathway signaling. Identifying GLI protein regulators is critical for the development of targeted therapies that can improve patient outcomes. This study aimed to identify a novel protein that directly regulates the activity of GLI transcription factors in prostate cancer. We performed gene expression, cellular analyses, and reporter assays to demonstrate that DAX1 (dosage-sensitive sex reversal adrenal hypoplasia congenital critical region on X chromosome, gene 1) interacts with GLI1 and GLI2, the master regulators of Hh signaling. Interestingly, DAX1 overexpression significantly inhibited Hh signaling by reducing GLI1 and GLI2 activity, prostate cancer cell proliferation, and viability. Our results shed light on a novel regulatory mechanism of Hh signaling in prostate cancer cells. The interaction between DAX1 and GLI transcription factors provides insight into the complex regulation of Hh signaling in prostate cancer. Given the importance of Hh signaling in prostate cancer progression, targeting DAX1–GLI interactions may represent a promising therapeutic approach against prostate cancer. Overall, this study provides new insights into the regulation of the Hh pathway and its role in prostate cancer progression. The findings suggest that DAX1 could serve as a potential therapeutic target for the treatment of prostate cancer.