Schistosomiasis is a disease of great medical and veterinary importance in tropical and subtropical regions, caused by parasitic flatworms of the genus Schistosoma (subclass Digenea). Following major water development schemes in the 1980s, schistosomiasis has become an important parasitic disease of children living in the Senegal River Basin (SRB). During molecular parasitological surveys, nuclear and mitochondrial markers revealed unexpected natural interactions between a bovine and human Schistosoma species: S. bovis and S. haematobium, respectively. Hybrid schistosomes recovered from the urine and faeces of children and the intermediate snail hosts of both parental species, Bulinus truncatus and B. globosus, presented a nuclear ITS rRNA sequence identical to S. haematobium, while the partial mitochondrial cox1 sequence was identified as S. bovis. Molecular data suggest that the hybrids are not 1st generation and are a result of parental and/or hybrid backcrosses, indicating a stable hybrid zone. Larval stages with the reverse genetic profile were also found and are suggested to be F1 progeny. The data provide indisputable evidence for the occurrence of bidirectional introgressive hybridization between a bovine and a human Schistosoma species. Hybrid species have been found infecting B. truncatus, a snail species that is now very abundant throughout the SRB. The recent increase in urinary schistosomiasis in the villages along the SRB could therefore be a direct effect of the increased transmission through B. truncatus. Hybridization between schistosomes under laboratory conditions has been shown to result in heterosis (higher fecundity, faster maturation time, wider intermediate host spectrum), having important implications on disease prevalence, pathology and treatment. If this new hybrid exhibits the same hybrid vigour, it could develop into an emerging pathogen, necessitating further control strategies in zones where both parental species overlap. Schistosome blood flukes cause significant disease in humans and their livestock in tropical and subtropical regions of the world. They have a two host-life cycle with a sexual stage within the mammalian host and are transmitted through water contact. Understanding the biology of these dioecious parasites is essential for developing strategies for control of schistosomiasis. Hybridization between schistosome species can occur, but in most cases host specificity and ecology are thought to maintain species barriers. Here, we report on the emergence of a new hybrid strain of schistosome found in northern Senegalese children, resulting from introgressive hybridization between a bovine and human parasite. This situation may have arisen due to the increased number of water contact sites commonly used by both cattle and people linked to recent major water development projects. Our findings have come to light due to optimized sampling and genotyping techniques of individual schistosome larval stages. Gene exchange following hybridization can lead to phenotypic innovations that can ultimately lead to the emergence of new diseases. The impact on disease epidemiology is only now unfolding, and it is essential to monitor the situation closely and move swiftly to control this rapidly evolving situation.

The phylogenetic relationships of 46 echinoids, with representatives from 13 of the 14 ordinal-level clades and about 70% of extant families commonly recognized, have been established from 3 genes (3,226 alignable bases) and 119 morphological characters. Morphological and molecular estimates are similar enough to be considered suboptimal estimates of one another, and the combined data provide a tree that, when calibrated against the fossil record, provides paleontological estimates of divergence times and completeness of their fossil record. The order of branching on the cladogram largely agrees with the stratigraphic order of first occurrences and implies that their fossil record is more than 85% complete at family level and at a resolution of 5-Myr time intervals. Molecular estimates of divergence times derived from applying both molecular clock and relaxed molecular clock models are concordant with estimates based on the fossil record in up to 70% of cases, with most concordant results obtained using Sanderson's semiparametric penalized likelihood method and a logarithmic-penalty function. There are 3 regions of the tree where molecular and fossil estimates of divergence time consistently disagree. Comparison with results obtained when molecular divergence dates are estimated from the combined (morphology + gene) tree suggests that errors in phylogenetic reconstruction explain only one of these. In another region the error most likely lies with the paleontological estimates because taxa in this region are demonstrated to have a very poor fossil record. In the third case, morphological and paleontological evidence is much stronger, and the topology for this part of the molecular tree differs from that derived from the combined data. Here the cause of the mismatch is unclear but could be methodological, arising from marked inequality of molecular rates. Overall, the level of agreement reached between these different data and methodological approaches leads us to believe that careful application of likelihood and Bayesian methods to molecular data provides realistic divergence time estimates in the majority of cases (almost 80% in this specific example), thus providing a remarkably well-calibrated phylogeny of a character-rich clade of ubiquitous marine benthic invertebrates.

Author Summary Schistosome blood flukes are transmitted through water contact and cause a severe debilitating disease in humans and their livestock. Understanding the biology and epidemiology of these parasites is essential to enable the development of better control strategies in endemic areas. Several species of schistosome exist and species barriers are normally maintained by differences in ecology, host specificity, and evolutionary history. However, hybridization between closely related species can occur if parasites infect the same definitive host. Here we report on the introgression between a human ( S. haematobium ) and two ruminant schistosomes ( S. bovis and S. curassoni ), the prevalence and distribution of their hybrids and the novel evidence for the presence of S. haematobium / S. curassoni hybrids in Senegalese children. Modern sampling and genotyping techniques have brought to light the extent of these hybridization events which could have been facilitated by the natural progression in farming, population (both human and livestock) movements and expansion, as well as changes in snail distribution, creating areas of sympatric transmission. Hybridization can lead to phenotypic characteristics that can influence disease epidemiology and control success, highlighting the importance of monitoring these evolving hybrid zones.