This study used elemental and stable isotope composition to characterize Slovenian truffles and used multi-variate statistical analysis to classify truffles according to species and geographical origin. Despite the fact that the Slovenian truffles shared some similar characteristics with the samples originating from other countries, differences in the element concentrations suggest that respective truffle species may respond selectively to nutrients from a certain soil type under environmental and soil conditions. Cross-validation resulted in a 77% correct classification rate for determining the geographical origin and a 74% correct classification rate to discriminate between species. The critical parameters for geographical origin discriminations were Sr, Ba, V, Pb, Ni, Cr, Ba/Ca and Sr/Ca ratios, while from stable isotopes δ¹⁸O and δ¹³C values are the most important. The key variables that distinguish T. magnatum from other species are the levels of V and Zn and δ¹⁵N values. Tuber aestivum can be separated based on the levels of Ni, Cr, Mn, Mg, As, and Cu. This preliminary study indicates the possibility to differentiate truffles according to their variety and geographical origin and suggests widening the scope to include stable strontium isotopes.

This paper summarises the isotopic characteristics, i.e., oxygen and hydrogen isotopes, of Slovenian milk and its major constituents: water, casein, and lactose. In parallel, the stable oxygen isotope ratios of cow, sheep, and goat’s milk were compared. Oxygen stable isotope ratios in milk water show seasonal variability and are also ¹⁸O enriched in relation to animal drinking water. The δ¹⁸O_water values were higher in sheep and goat’s milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal’s thermoregulatory physiologies. The relationship between δ¹⁸O_milk and δ¹⁸O_lactose is an indication that even at lower amounts (>7%) of added water to milk can be determined. This procedure once validated on an international scale could become a reference method for the determination of milk adulteration with water.

This work presents the first use of Sr isotope ratios for determining the provenance of bovine milk from different regions of Slovenia. The analytical protocol for the determination of ⁸⁷Sr/⁸⁶Sr isotope ratio was optimised and applied to authentic milk samples. Considerable variability of ⁸⁷Sr/⁸⁶Sr ratios found in Slovenian milk reflects the substantial heterogeneity of the geological background of its origin. The results, although promising, cannot discount possible inter-annual or annual variation of the Sr isotopic composition of milk. The ⁸⁷Sr/⁸⁶Sr ratios of groundwater and surface waters are in good correlation with milk, indicating that the Sr isotopic fingerprint in milk is reflective of cow drinking water. The ⁸⁷Sr/⁸⁶Sr ratio has the potential to distinguish between different milk production areas as long as these areas are characterised by geo-lithology. Discriminant analysis (DA) incorporating the elemental composition and stable isotopes of light elements showed that ⁸⁷Sr/⁸⁶Sr ratio together with δ¹³C_cas and δ¹⁵N_cas values have the main discrimination power to distinguish the Quaternary group (group 6) from the others. Group 1 (Cretaceous: Carbonate Rocks and Flysch) is associated with Br content, 1/Sr and δ¹⁸O_w values. The overall prediction ability was found to be 63.5%. Pairwise comparisons using OPLS-DA confirmed that diet and geologic parameters are important for the separation.

We appreciate Dr Horacek’s interest in our paper and his feedback