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Cristina García-Fontana
CIBERFES, Instituto de Salud Carlos III, 28029 Madrid, Spain

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Journal article
Published: 20 May 2021 in Journal of Clinical Medicine
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Osteoglycin (OGN) could be a biomarker of mild kidney function impairment in type 2 diabetes (T2D). Our study aimed to determine the association between serum OGN and impaired kidney function risk in T2D patients and to analyze its potential role as an estimator of kidney disturbances in this population. This cross-sectional study included 147 T2D patients (65 ± 8 years, 58.5% males), and 75 healthy controls (63 ± 10 years, 36% males). Circulating OGN levels were determined by ELISA. Linear regression modeling was performed to determine the variables influencing circulating OGN, and an ROC curve was plotted to assess the usefulness of OGN as an estimator of diabetic kidney disease risk. Circulating OGN was significantly increased in T2D patients compared to controls (18.41 (14.45–23.27) ng/mL vs. 8.74 (7.03–12.35) ng/mL; p< 0.001). We found a progressive increase in serum OGN according to the severity of kidney impairment in T2D patients (normal kidney function: 16.14 (12.13–20.48) ng/mL; mildly impaired kidney function: 19.15 (15.78–25.90) ng/mL; moderate impaired kidney function: 21.80 (15.06–29.22) ng/mL; p = 0.006). Circulating OGN was an independent estimator of mildly impaired kidney function risk in T2D patients. We suggest that serum OGN could act as an albuminuria-independent biomarker of incipient kidney dysfunction in T2D patients.

ACS Style

Sheila González-Salvatierra; Cristina García-Fontana; Francisco Andújar-Vera; Alejandro Grau-Perales; Luis Martínez-Heredia; María Avilés-Pérez; María Hayón-Ponce; Iván Iglesias-Baena; Blanca Riquelme-Gallego; Manuel Muñoz-Torres; Beatriz García-Fontana. Osteoglycin as a Potential Biomarker of Mild Kidney Function Impairment in Type 2 Diabetes Patients. Journal of Clinical Medicine 2021, 10, 2209 .

AMA Style

Sheila González-Salvatierra, Cristina García-Fontana, Francisco Andújar-Vera, Alejandro Grau-Perales, Luis Martínez-Heredia, María Avilés-Pérez, María Hayón-Ponce, Iván Iglesias-Baena, Blanca Riquelme-Gallego, Manuel Muñoz-Torres, Beatriz García-Fontana. Osteoglycin as a Potential Biomarker of Mild Kidney Function Impairment in Type 2 Diabetes Patients. Journal of Clinical Medicine. 2021; 10 (10):2209.

Chicago/Turabian Style

Sheila González-Salvatierra; Cristina García-Fontana; Francisco Andújar-Vera; Alejandro Grau-Perales; Luis Martínez-Heredia; María Avilés-Pérez; María Hayón-Ponce; Iván Iglesias-Baena; Blanca Riquelme-Gallego; Manuel Muñoz-Torres; Beatriz García-Fontana. 2021. "Osteoglycin as a Potential Biomarker of Mild Kidney Function Impairment in Type 2 Diabetes Patients." Journal of Clinical Medicine 10, no. 10: 2209.

Review
Published: 21 April 2021 in International Journal of Molecular Sciences
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Hypophosphatasia (HPP) is a rare genetic disease characterized by a decrease in the activity of tissue non-specific alkaline phosphatase (TNSALP). TNSALP is encoded by the ALPL gene, which is abundantly expressed in the skeleton, liver, kidney, and developing teeth. HPP exhibits high clinical variability largely due to the high allelic heterogeneity of the ALPL gene. HPP is characterized by multisystemic complications, although the most common clinical manifestations are those that occur in the skeleton, muscles, and teeth. These complications are mainly due to the accumulation of inorganic pyrophosphate (PPi) and pyridoxal-5′-phosphate (PLP). It has been observed that the prevalence of mild forms of the disease is more than 40 times the prevalence of severe forms. Patients with HPP present at least one mutation in the ALPL gene. However, it is known that there are other causes that lead to decreased alkaline phosphatase (ALP) levels without mutations in the ALPL gene. Although the phenotype can be correlated with the genotype in HPP, the prediction of the phenotype from the genotype cannot be made with complete certainty. The availability of a specific enzyme replacement therapy for HPP undoubtedly represents an advance in therapeutic strategy, especially in severe forms of the disease in pediatric patients.

ACS Style

Juan Villa-Suárez; Cristina García-Fontana; Francisco Andújar-Vera; Sheila González-Salvatierra; Tomás de Haro-Muñoz; Victoria Contreras-Bolívar; Beatriz García-Fontana; Manuel Muñoz-Torres. Hypophosphatasia: A Unique Disorder of Bone Mineralization. International Journal of Molecular Sciences 2021, 22, 4303 .

AMA Style

Juan Villa-Suárez, Cristina García-Fontana, Francisco Andújar-Vera, Sheila González-Salvatierra, Tomás de Haro-Muñoz, Victoria Contreras-Bolívar, Beatriz García-Fontana, Manuel Muñoz-Torres. Hypophosphatasia: A Unique Disorder of Bone Mineralization. International Journal of Molecular Sciences. 2021; 22 (9):4303.

Chicago/Turabian Style

Juan Villa-Suárez; Cristina García-Fontana; Francisco Andújar-Vera; Sheila González-Salvatierra; Tomás de Haro-Muñoz; Victoria Contreras-Bolívar; Beatriz García-Fontana; Manuel Muñoz-Torres. 2021. "Hypophosphatasia: A Unique Disorder of Bone Mineralization." International Journal of Molecular Sciences 22, no. 9: 4303.

Journal article
Published: 18 August 2020 in Water
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In wastewater treatment plants, most microbial characterization has focused on bacterial, archaeal, and fungal populations. Due to the difficult isolation, quantification, and identification of viruses, only a limited number of virome studies associated with wastewater treatment plants have been carried out. However, the virus populations play an important role in the microbial dynamics in wastewater treatment systems and the biosafety of effluents. In this work, the viral members present in influent wastewater, mixed liquor (aerobic bioreactor), excess sludge, and effluent water of a conventional activated sludge system for the treatment of urban wastewater were identified. Viral members were observed by transmission electron microscopy and studied through next-generation sequencing studies. The results showed the dominance of bacteriophages in the viral community in all samples, with the dominant viral phylotype classified as Escherichia coli O157 typing phage 7. Moreover, different human viruses, such as Cynomolgus cytomegalovirus and Gammaherpesvirus, were also detected.

ACS Style

Cristina García-Fontana; Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maria Vela-Cano; Jesus Gonzalez-Lopez. Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain. Water 2020, 12, 2316 .

AMA Style

Cristina García-Fontana, Alejandro Rodriguez-Sanchez, Barbara Muñoz-Palazon, Alejandro Gonzalez-Martinez, Maria Vela-Cano, Jesus Gonzalez-Lopez. Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain. Water. 2020; 12 (8):2316.

Chicago/Turabian Style

Cristina García-Fontana; Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maria Vela-Cano; Jesus Gonzalez-Lopez. 2020. "Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain." Water 12, no. 8: 2316.

Preprint content
Published: 26 March 2020
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Desiccation-tolerant plants are able to survive for extended periods of time in the absence of water. The molecular understanding of the mechanisms used by these plants to resist droughts can be of great value for the improvement of tolerance of sensitive plants with agricultural interest. This understanding is especially relevant in an environment that tends to increase the number and intensity of droughts. The combination of certain microorganisms with drought-sensitive plants can improve their tolerance to water scarcity. One of these bacteria is Microbacterium sp. 3J1, an actinobacteria able to protect pepper plants from drought. In this study we describe the proteome of the interaction of Microbacterium sp. 3J1 with pepper plants during droughts. We also compare this proteome with the proteome found in desiccation-tolerant plants. In addition, we studied the proteome of Microbacterium sp. 3J1 subjected to drought to analyze its contribution to the plant-microbe interaction. We describe those mechanisms shared by desiccation-tolerant plants and sensitive plants protected by microorganisms focusing on protection against oxidative stress, and production of compatible solutes, plant hormones, and other more specific proteins.IMPORTANCEMaintaining agricultural production under greater number and intensity of droughts is one of the main global challenges. Some plants can survive in the absence of water for extended periods of time. The molecular understanding of the mechanisms used by these plants to resist droughts is of great interest for the development of new strategies to face this challenge. Some microorganisms protect sensitive plants to some extent from droughts. Microbacterium sp. 3J1, is an actinobacteria able to protect pepper plants from drought. In this study we describe the different protein profile under drought used by the plant during the interaction with the microorganism and compare it with the one presented by desiccation-tolerant plants and with the one presented by Microbacterium sp. 3J1 to analyze its contribution to the plant-microbe interaction. We describe those mechanisms focusing on protection against oxidative stress, and production of compatible solutes, plant-hormones, and other more specific proteins.

ACS Style

Cristina García-Fontana; Juan Ignacio Vílchez; Maximino Manzanera. Proteome comparison between natural desiccation-tolerant plants and drought-protected Caspicum annuum plants by Microbacterium sp. 3J1. 2020, 1 .

AMA Style

Cristina García-Fontana, Juan Ignacio Vílchez, Maximino Manzanera. Proteome comparison between natural desiccation-tolerant plants and drought-protected Caspicum annuum plants by Microbacterium sp. 3J1. . 2020; ():1.

Chicago/Turabian Style

Cristina García-Fontana; Juan Ignacio Vílchez; Maximino Manzanera. 2020. "Proteome comparison between natural desiccation-tolerant plants and drought-protected Caspicum annuum plants by Microbacterium sp. 3J1." , no. : 1.

Journal article
Published: 11 September 2019 in Scientific Reports
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Pseudomonas aeruginosais an opportunistic human pathogen causing infections in a variety of plant and animal hosts. The genemcpB, part of the chemosensory gene cluster II, encodes a soluble chemoreceptor whose function remains unknown. Previous studies show that thecheB2gene, also located in the chemosensory cluster II, is involved in a specific response during infection and it is required for full pathogenicity ofP.aeruginosa. To determine whether the McpB (or Aer2) chemoreceptor is involved in virulence processes, we generated amcpBmutant and tested its phenotype using a virulence-measuring system. This system was developed by our group and is based on different bioassays using organisms living at different soil trophic levels, including microbial, nematode, arthropod, annelid, and plant model systems. The deletion ofmcpBresulted in an attenuation of bacterial virulence in different infection models, and wild-type virulence was restored following genetic complementation of the mutant strain. Our study indicates that the McpB chemoreceptor is linked to virulence processes and may constitute the basis for the development of alternative strategies against this pathogen.

ACS Style

Cristina García-Fontana; Juan I. Vílchez; Marta González-Requena; Jesús González-López; Tino Krell; Miguel A. Matilla; Maximino Manzanera. The involvement of McpB chemoreceptor from Pseudomonas aeruginosa PAO1 in virulence. Scientific Reports 2019, 9, 1 -11.

AMA Style

Cristina García-Fontana, Juan I. Vílchez, Marta González-Requena, Jesús González-López, Tino Krell, Miguel A. Matilla, Maximino Manzanera. The involvement of McpB chemoreceptor from Pseudomonas aeruginosa PAO1 in virulence. Scientific Reports. 2019; 9 (1):1-11.

Chicago/Turabian Style

Cristina García-Fontana; Juan I. Vílchez; Marta González-Requena; Jesús González-López; Tino Krell; Miguel A. Matilla; Maximino Manzanera. 2019. "The involvement of McpB chemoreceptor from Pseudomonas aeruginosa PAO1 in virulence." Scientific Reports 9, no. 1: 1-11.

Journal article
Published: 02 July 2019 in Scientific Reports
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Hypophosphatasia (HPP) is a genetic disease caused by one or several mutations in ALPL gene encoding the tissue-nonspecific alkaline phosphatase affecting the mineralization process. Due to its low prevalence and lack of recognition, this metabolic disorder is generally confused with other more frequent bone disorders. An assessment of serum total alkaline phosphatase (ALP) levels was performed in 78,590 subjects. Pyridoxal-5′-phosphate (PLP) concentrations were determined and ALPL gene was sequenced in patients potentially affected by HPP. Functional validation of the novel mutations found was performed using a cell-based assay. Our results showed persistently low serum ALP levels in 0.12% of subjects. Among the studied subjects, 40% presented with HPP-related symptoms. Nine of them (~28%) had a history of fractures, 5 (~16%) subjects showed chondrocalcinosis and 4 (~13%) subjects presented with dental abnormalities. Eleven subjects showed increased PLP concentrations. Seven of them showed ALPL gene mutations (2 of the mutations corresponded to novel genetic variants). In summary, we identified two novel ALPL gene mutations associated with adult HPP. Using this protocol, almost half of the studied patients were diagnosed with HPP. Based on these results, the estimated prevalence of mild HPP in Spain could be up to double than previously reported.

ACS Style

Cristina Garcia Fontana; Juan M. Villa-Suárez; Francisco Andújar-Vera; Sheila González-Salvatierra; Gonzalo Martínez-Navajas; Pedro J. Real; José M. Gómez Vida; Tomás De Haro; Beatriz García Fontana; Manuel Muñoz-Torres. Epidemiological, Clinical and Genetic Study of Hypophosphatasia in A Spanish Population: Identification of Two Novel Mutations in The Alpl Gene. Scientific Reports 2019, 9, 1 -11.

AMA Style

Cristina Garcia Fontana, Juan M. Villa-Suárez, Francisco Andújar-Vera, Sheila González-Salvatierra, Gonzalo Martínez-Navajas, Pedro J. Real, José M. Gómez Vida, Tomás De Haro, Beatriz García Fontana, Manuel Muñoz-Torres. Epidemiological, Clinical and Genetic Study of Hypophosphatasia in A Spanish Population: Identification of Two Novel Mutations in The Alpl Gene. Scientific Reports. 2019; 9 (1):1-11.

Chicago/Turabian Style

Cristina Garcia Fontana; Juan M. Villa-Suárez; Francisco Andújar-Vera; Sheila González-Salvatierra; Gonzalo Martínez-Navajas; Pedro J. Real; José M. Gómez Vida; Tomás De Haro; Beatriz García Fontana; Manuel Muñoz-Torres. 2019. "Epidemiological, Clinical and Genetic Study of Hypophosphatasia in A Spanish Population: Identification of Two Novel Mutations in The Alpl Gene." Scientific Reports 9, no. 1: 1-11.

Journal article
Published: 01 September 2018 in Ecotoxicology and Environmental Safety
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Polycyclic aromatic hydrocarbons (PAHs) are widely distributed in polluted environments and are included in the priority list of toxic compounds. Previous studies have shown that the fungus Penicillium oxalicum, isolated from a hydrocarbon-polluted pond, has a great capability to transform different PAHs in short periods under submerged fermentation conditions. Although cytochrome p450s (CYPs) seems to be the main responsible enzyme in this process, changes in proteome profile remains poorly understood. The aim of this work was to characterise molecular disturbances in the cytosolic and microsomal sub-proteomes of P. oxalicum by applying two-dimensional (2D) gel electrophoresis and label-free quantitative proteomics during anthracene biodegradation. Our results showed that by using 2D-gels, 10 and 8 differential proteins were over-expressed in the cytosolic and microsomal fractions, respectively. Most of them were related to stress response. Shotgun proteomics allowed the identification of 158 and 174 unique protein species that differentially accumulated during anthracene biotransformation, such as CYPs, epoxide hydrolases and transferases enzymes, belonging to Phase I and Phase II of the metabolism of xenobiotics, contributing to the anthracene biodegradation pathway. These results confirm the biological significance of ascomycetes fungi the rol of CYPs on biodegradation and the need of a deeper knowledge on fungal proteomics for the application of the appropriate microorganisms in biodegradation processes.

ACS Style

R. Lucero Camacho-Morales; Cristina García-Fontana; Joaquín Fernández-Irigoyen; Enrique Santamaría; Jesús González-López; Maximino Manzanera; Elisabet Aranda. Anthracene drives sub-cellular proteome-wide alterations in the degradative system of Penicillium oxalicum. Ecotoxicology and Environmental Safety 2018, 159, 127 -135.

AMA Style

R. Lucero Camacho-Morales, Cristina García-Fontana, Joaquín Fernández-Irigoyen, Enrique Santamaría, Jesús González-López, Maximino Manzanera, Elisabet Aranda. Anthracene drives sub-cellular proteome-wide alterations in the degradative system of Penicillium oxalicum. Ecotoxicology and Environmental Safety. 2018; 159 ():127-135.

Chicago/Turabian Style

R. Lucero Camacho-Morales; Cristina García-Fontana; Joaquín Fernández-Irigoyen; Enrique Santamaría; Jesús González-López; Maximino Manzanera; Elisabet Aranda. 2018. "Anthracene drives sub-cellular proteome-wide alterations in the degradative system of Penicillium oxalicum." Ecotoxicology and Environmental Safety 159, no. : 127-135.

Research article
Published: 21 June 2018 in PLOS ONE
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Cardiovascular diseases are a health problem throughout the world, especially in people with diabetes. The identification of cardiovascular disease biomarkers can improve risk stratification. Sclerostin is a modulator of the Wnt/β-catenin signalling pathway in different tissues, and it has recently been linked to vascular biology. The current study aimed to evaluate the relationship between circulating sclerostin levels and cardiovascular and non-cardiovascular mortality in individuals with and without type 2 diabetes. We followed up a cohort of 130 participants (mean age 56.8 years; 48.5% females; 75 with type 2 diabetes; 46 with prevalent cardiovascular disease) in which serum sclerostin levels were measured at the baseline. Time to death (both of cardiovascular and non-cardiovascular causes) was assessed to establish the relationship between sclerostin and mortality. We found that serum sclerostin concentrations were significantly higher in patients with prevalent cardiovascular disease (p<0.001), and independently associated with cardiovascular mortality (p = 0.008), showing sclerostin to be a stronger predictor of mortality than other classical risk factors (area under the curve = 0.849 vs 0.823). The survival analysis showed that an increase of 10 pmol/L in the serum sclerostin level resulted in a 31% increase in cardiovascular mortality. However, no significant association was observed between sclerostin levels and non-cardiovascular mortality (p = 0.346). From these results, we conclude that high sclerostin levels are related to mortality due to cardiovascular causes. The clinical implication of these findings is based on the possible use of serum sclerostin as a new biomarker of cardiovascular mortality risk in order to establish preventive strategies.

ACS Style

Cristina Novo-Rodríguez; Beatriz García-Fontana; Juan De Dios Luna-Del Castillo; Francisco Andújar-Vera; Verónica Ávila-Rubio; Cristina García-Fontana; Sonia Morales-Santana; Pedro Rozas-Moreno; Manuel Muñoz-Torres. Circulating levels of sclerostin are associated with cardiovascular mortality. PLOS ONE 2018, 13, e0199504 .

AMA Style

Cristina Novo-Rodríguez, Beatriz García-Fontana, Juan De Dios Luna-Del Castillo, Francisco Andújar-Vera, Verónica Ávila-Rubio, Cristina García-Fontana, Sonia Morales-Santana, Pedro Rozas-Moreno, Manuel Muñoz-Torres. Circulating levels of sclerostin are associated with cardiovascular mortality. PLOS ONE. 2018; 13 (6):e0199504.

Chicago/Turabian Style

Cristina Novo-Rodríguez; Beatriz García-Fontana; Juan De Dios Luna-Del Castillo; Francisco Andújar-Vera; Verónica Ávila-Rubio; Cristina García-Fontana; Sonia Morales-Santana; Pedro Rozas-Moreno; Manuel Muñoz-Torres. 2018. "Circulating levels of sclerostin are associated with cardiovascular mortality." PLOS ONE 13, no. 6: e0199504.

Original research article
Published: 22 December 2016 in Frontiers in Microbiology
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The DNA molecule is associated with the role of encoding information required to produce RNA which is translated into proteins needed by the cell. This encoding involves information transmission to offspring or to other organisms by horizontal transfer. However, despite the abundance of this molecule in both the cell and the environment, its physiological role seems to be restricted mainly to that of a coding and inheritance molecule. In this paper, we report a new physiological role for the DNA molecule as involved in protection against desiccation, in addition to its well-established main information transfer and other recently reported functions such as bio-film formation in eDNA form. Desiccation-tolerant microorganisms such as Microbacterium sp. 3J1 significantly upregulate genes involved in DNA synthesis to produce DNA as part of their defensive mechanisms to protect protein structures and functions from drying according to RNA-seq analysis. We have observed the intracellular overproduction of DNA in two desiccation-tolerant microorganisms, Microbacterium sp. 3J1 and Arthrobacter siccitolerans 4J27, in response to desiccation signals. In addition, this conclusion can be made from our observations that synthetic DNA protects two proteins from drying and when part of a xeroprotectant preparation, DNA from various organisms including desiccation-sensitive species, does the same. Removal of DNA by nuclease treatment results in absence of this additive protective effect. We validated this role in biochemical and biophysical assays in proteins and occurs in trans even with short, single chains of synthetically produced DNA.

ACS Style

Cristina Garcia Fontana; Juan J. Narváez-Reinaldo; Francisco Castillo; Jesús González-López; Irene Luque; Maximino Manzanera. A New Physiological Role for the DNA Molecule as a Protector against Drying Stress in Desiccation-Tolerant Microorganisms. Frontiers in Microbiology 2016, 7, 2066 .

AMA Style

Cristina Garcia Fontana, Juan J. Narváez-Reinaldo, Francisco Castillo, Jesús González-López, Irene Luque, Maximino Manzanera. A New Physiological Role for the DNA Molecule as a Protector against Drying Stress in Desiccation-Tolerant Microorganisms. Frontiers in Microbiology. 2016; 7 ():2066.

Chicago/Turabian Style

Cristina Garcia Fontana; Juan J. Narváez-Reinaldo; Francisco Castillo; Jesús González-López; Irene Luque; Maximino Manzanera. 2016. "A New Physiological Role for the DNA Molecule as a Protector against Drying Stress in Desiccation-Tolerant Microorganisms." Frontiers in Microbiology 7, no. : 2066.

Original research article
Published: 30 September 2016 in Frontiers in Microbiology
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A collection of desiccation-tolerant xeroprotectant-producing microorganisms was screened for their ability to protect plants against drought, and their role as plant growth-promoting rhizobacteria was investigated in two different crops (tomato and pepper). The most commonly described biochemical mechanisms for plant protection against drought by microorganisms including the production of phytohormones, antioxidants and xeroprotectants were analyzed. In particular, the degree of plant protection against drought provided by these microorganisms was characterized. After studying the findings and comparing them with results of the closest taxonomic relatives at the species and strain levels, we propose that trehalose produced by these microorganisms is correlated with their ability to protect plants against drought. This proposal is based on the increased protection of plants against drought by the desiccation-sensitive microorganism Pseudomonas putida KT2440, which expresses the otsAB genes for trehalose biosynthesis in trans.

ACS Style

Juan I. Vílchez; Cristina Garcia Fontana; Desireé Román-Naranjo; Jesús González-López; Maximino Manzanera. Plant Drought Tolerance Enhancement by Trehalose Production of Desiccation-Tolerant Microorganisms. Frontiers in Microbiology 2016, 7, 1577 .

AMA Style

Juan I. Vílchez, Cristina Garcia Fontana, Desireé Román-Naranjo, Jesús González-López, Maximino Manzanera. Plant Drought Tolerance Enhancement by Trehalose Production of Desiccation-Tolerant Microorganisms. Frontiers in Microbiology. 2016; 7 ():1577.

Chicago/Turabian Style

Juan I. Vílchez; Cristina Garcia Fontana; Desireé Román-Naranjo; Jesús González-López; Maximino Manzanera. 2016. "Plant Drought Tolerance Enhancement by Trehalose Production of Desiccation-Tolerant Microorganisms." Frontiers in Microbiology 7, no. : 1577.

Journal article
Published: 27 August 2015 in Genome Announcements
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The genome sequence for Microbacterium sp. strain 3J1, a desiccation-tolerant organism isolated from the Nerium oleander rhizosphere, is reported here. The genome is estimated to be approximately 3.5 Mb in size, with an average G+C content of 67.7% and a predicted number of protein-coding sequences of 3,310.

ACS Style

Maximino Manzanera; Cristina García-Fontana; Juan Ignacio Vílchez; Juan Jesús Narváez-Reinaldo; Jesús González-López. Genome Sequence of Microbacterium sp. Strain 3J1, a Highly Desiccation-Tolerant Bacterium That Promotes Plant Growth. Genome Announcements 2015, 3, e00713-15 .

AMA Style

Maximino Manzanera, Cristina García-Fontana, Juan Ignacio Vílchez, Juan Jesús Narváez-Reinaldo, Jesús González-López. Genome Sequence of Microbacterium sp. Strain 3J1, a Highly Desiccation-Tolerant Bacterium That Promotes Plant Growth. Genome Announcements. 2015; 3 (4):e00713-15.

Chicago/Turabian Style

Maximino Manzanera; Cristina García-Fontana; Juan Ignacio Vílchez; Juan Jesús Narváez-Reinaldo; Jesús González-López. 2015. "Genome Sequence of Microbacterium sp. Strain 3J1, a Highly Desiccation-Tolerant Bacterium That Promotes Plant Growth." Genome Announcements 3, no. 4: e00713-15.

Journal article
Published: 25 June 2015 in Genome Announcements
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The genome sequence for Rhodococcus sp. 4J2A2, a newly described desiccation-tolerant strain that removes aromatic hydrocarbons, is reported here. The genome is estimated to be around 7.5 Mb in size, with an average G+C content of 60.77% and a predicted number of protein-coding sequences of 6,354.

ACS Style

Maximino Manzanera; Cristina García-Fontana; Juan Ignacio Vílchez; Jesús González-López. Genome Sequence of Rhodococcus sp. 4J2A2, a Desiccation-Tolerant Bacterium Involved in Biodegradation of Aromatic Hydrocarbons. Genome Announcements 2015, 3, e00592-15 .

AMA Style

Maximino Manzanera, Cristina García-Fontana, Juan Ignacio Vílchez, Jesús González-López. Genome Sequence of Rhodococcus sp. 4J2A2, a Desiccation-Tolerant Bacterium Involved in Biodegradation of Aromatic Hydrocarbons. Genome Announcements. 2015; 3 (3):e00592-15.

Chicago/Turabian Style

Maximino Manzanera; Cristina García-Fontana; Juan Ignacio Vílchez; Jesús González-López. 2015. "Genome Sequence of Rhodococcus sp. 4J2A2, a Desiccation-Tolerant Bacterium Involved in Biodegradation of Aromatic Hydrocarbons." Genome Announcements 3, no. 3: e00592-15.

Journal article
Published: 25 June 2015 in Genome Announcements
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Arthrobacter koreensis 5J12A is a desiccation-tolerant organism isolated from the Nerium oleander rhizosphere. Here, we report its genome sequence, which may shed light on its role in plant growth promotion. This is believed to be the first published genome of a desiccation-tolerant plant growth promoter from the genus Arthrobacter .

ACS Style

Maximino Manzanera; Juan Jesús Narváez-Reinaldo; Cristina García-Fontana; Juan Ignacio Vílchez; Jesús González-López. Genome Sequence of Arthrobacter koreensis 5J12A, a Plant Growth-Promoting and Desiccation-Tolerant Strain. Genome Announcements 2015, 3, e00648-15 .

AMA Style

Maximino Manzanera, Juan Jesús Narváez-Reinaldo, Cristina García-Fontana, Juan Ignacio Vílchez, Jesús González-López. Genome Sequence of Arthrobacter koreensis 5J12A, a Plant Growth-Promoting and Desiccation-Tolerant Strain. Genome Announcements. 2015; 3 (3):e00648-15.

Chicago/Turabian Style

Maximino Manzanera; Juan Jesús Narváez-Reinaldo; Cristina García-Fontana; Juan Ignacio Vílchez; Jesús González-López. 2015. "Genome Sequence of Arthrobacter koreensis 5J12A, a Plant Growth-Promoting and Desiccation-Tolerant Strain." Genome Announcements 3, no. 3: e00648-15.

Research article
Published: 01 July 2014 in Microbial Biotechnology
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The reason for the existence of complex sensor kinases is little understood but thought to lie in the capacity to respond to multiple signals. The complex, seven‐domain sensor kinase TodS controls in concert with the TodT response regulator the expression of the toluene dioxygenase pathway in Pseudomonas putida F1 and DOT‐T1E. We have previously shown that some aromatic hydrocarbons stimulate TodS activity whereas others behave as antagonists. We show here that TodS responds in addition to the oxidative agent menadione. Menadione but no other oxidative agent tested inhibited TodS activity in vitro and reduced PtodX expression in vivo. The menadione signal is incorporated by a cysteine‐dependent mechanism. The mutation of the sole conserved cysteine of TodS (C320) rendered the protein insensitive to menadione. We evaluated the mutual opposing effects of toluene and menadione on TodS autophosphorylation. In the presence of toluene, menadione reduced TodS activity whereas toluene did not stimulate activity in the presence of menadione. It was shown by others that menadione increases expression of glucose metabolism genes. The opposing effects of menadione on glucose and toluene metabolism may be partially responsible for the interwoven regulation of both catabolic pathways. This work provides mechanistic detail on how complex sensor kinases integrate different types of signal molecules.

ACS Style

Hortencia Silva‐Jiménez; Alvaro Ortega; Cristina Garcia Fontana; Juan L. Ramos; Tino Krell. Multiple signals modulate the activity of the complex sensor kinase T od S. Microbial Biotechnology 2014, 8, 103 -115.

AMA Style

Hortencia Silva‐Jiménez, Alvaro Ortega, Cristina Garcia Fontana, Juan L. Ramos, Tino Krell. Multiple signals modulate the activity of the complex sensor kinase T od S. Microbial Biotechnology. 2014; 8 (1):103-115.

Chicago/Turabian Style

Hortencia Silva‐Jiménez; Alvaro Ortega; Cristina Garcia Fontana; Juan L. Ramos; Tino Krell. 2014. "Multiple signals modulate the activity of the complex sensor kinase T od S." Microbial Biotechnology 8, no. 1: 103-115.

Research article
Published: 08 April 2014 in Science Signaling
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Methyltransferases of the CheR family and methylesterases of the CheB family control chemoreceptor methylation, and this dynamic posttranslational modification is necessary for proper chemotaxis of bacteria. Studies with enterobacteria that contain a single CheR or CheB show that, in addition to binding at the methylation site, some chemoreceptors bind CheR or CheB through additional high-affinity sites at distinct pentapeptide sequences in the chemoreceptors. We investigated the recognition of chemoreceptors by CheR proteins in the human pathogen Pseudomonas aeruginosa PAO1. Of the four methyltransferases in PAO1, we detected an interaction only between CheR2 and the chemoreceptor methyl-accepting chemotaxis protein B (McpB), which contains the pentapeptide GWEEF at its carboxyl terminus. Furthermore, CheR2 was also the only paralog that methylated McpB in vitro, and deletion of the pentapeptide sequence abolished both the CheR2-McpB interaction and the methylation of McpB. When clustered according to protein sequence, bacterial CheR proteins form two distinct families—those that bind pentapeptide-containing chemoreceptors and those that do not. These two families are distinguished by an insertion of three amino acids in the β-subdomain of CheR. Deletion of this insertion in CheR2 prevented its interaction with and methylation of McpB. Pentapeptide-containing chemoreceptors are common to many bacteria species; thus, these short, distinct motifs may enable the specific assembly of signaling complexes that mediate different responses.

ACS Style

Cristina García-Fontana; Andrés Corral Lugo; Tino Krell. Specificity of the CheR2 Methyltransferase in Pseudomonas aeruginosa Is Directed by a C-Terminal Pentapeptide in the McpB Chemoreceptor. Science Signaling 2014, 7, ra34 -ra34.

AMA Style

Cristina García-Fontana, Andrés Corral Lugo, Tino Krell. Specificity of the CheR2 Methyltransferase in Pseudomonas aeruginosa Is Directed by a C-Terminal Pentapeptide in the McpB Chemoreceptor. Science Signaling. 2014; 7 (320):ra34-ra34.

Chicago/Turabian Style

Cristina García-Fontana; Andrés Corral Lugo; Tino Krell. 2014. "Specificity of the CheR2 Methyltransferase in Pseudomonas aeruginosa Is Directed by a C-Terminal Pentapeptide in the McpB Chemoreceptor." Science Signaling 7, no. 320: ra34-ra34.

Book chapter
Published: 29 March 2014 in Advanced Structural Safety Studies
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Isothermal titration calorimetry (ITC) is based on a simple titration of one ligand with another and the small heat changes caused by the molecular interaction are detected. From one ITC experiment the complete set of thermodynamic parameters of binding including association and dissociation constants as well as changes in enthalpy, entropy, and free energy can be derived. Using this technique almost any type of molecular interaction can be analyzed. Both ligands are in solution, and there is no need for their chemical derivatization. There are no limits as to the choice of the analysis buffer, and the analysis temperature can be set between 4 and 80 °C. This technique has been primarily applied to study the interaction between various proteins of Pseudomonas with small molecule ligands. In addition, ITC has been used to study the binding of Pseudomonas proteins to target DNA fragments.

ACS Style

Tino Krell; Jesús Lacal; Cristina García-Fontana; Hortencia Silva-Jiménez; Miriam Rico-Jiménez; Andrés Corral Lugo; José Antonio Reyes Darias; Juan-Luis Ramos. Characterization of Molecular Interactions Using Isothermal Titration Calorimetry. Advanced Structural Safety Studies 2014, 1149, 193 -203.

AMA Style

Tino Krell, Jesús Lacal, Cristina García-Fontana, Hortencia Silva-Jiménez, Miriam Rico-Jiménez, Andrés Corral Lugo, José Antonio Reyes Darias, Juan-Luis Ramos. Characterization of Molecular Interactions Using Isothermal Titration Calorimetry. Advanced Structural Safety Studies. 2014; 1149 ():193-203.

Chicago/Turabian Style

Tino Krell; Jesús Lacal; Cristina García-Fontana; Hortencia Silva-Jiménez; Miriam Rico-Jiménez; Andrés Corral Lugo; José Antonio Reyes Darias; Juan-Luis Ramos. 2014. "Characterization of Molecular Interactions Using Isothermal Titration Calorimetry." Advanced Structural Safety Studies 1149, no. : 193-203.

Protocol
Published: 29 March 2014 in Methods in Molecular Biology
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A primary driving force during bacterial evolution was the capacity to access compounds necessary for growth and survival. Since the species of the genus Pseudomonas are characterized by metabolic versatility, these bacteria have developed chemotactic behaviors towards a wide range of different compounds. The specificity of a chemotactic response is determined by the chemoreceptor, which is at the beginning of the signaling cascade and to which chemoattractants and chemorepellents bind. The number of chemoreceptor genes of Pseudomonas species is significantly higher than the average number in motile bacteria. Although some of the receptors have been annotated with a function, the cognate signal molecules for the majority of them still need to be identified. Different qualitative and quantitative methods are presented that can be used to study flagellum-mediated taxis.

ACS Style

José Antonio Reyes Darias; Cristina Garcia Fontana; Andrés Corral Lugo; Miriam Rico-Jiménez; Tino Krell. Qualitative and Quantitative Assays for Flagellum-Mediated Chemotaxis. Methods in Molecular Biology 2014, 1149, 87 -97.

AMA Style

José Antonio Reyes Darias, Cristina Garcia Fontana, Andrés Corral Lugo, Miriam Rico-Jiménez, Tino Krell. Qualitative and Quantitative Assays for Flagellum-Mediated Chemotaxis. Methods in Molecular Biology. 2014; 1149 ():87-97.

Chicago/Turabian Style

José Antonio Reyes Darias; Cristina Garcia Fontana; Andrés Corral Lugo; Miriam Rico-Jiménez; Tino Krell. 2014. "Qualitative and Quantitative Assays for Flagellum-Mediated Chemotaxis." Methods in Molecular Biology 1149, no. : 87-97.

Journal article
Published: 01 June 2013 in Journal of Biological Chemistry
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Chemosensory pathways are a major signal transduction mechanism in bacteria. CheR methyltransferases catalyze the methylation of the cytosolic signaling domain of chemoreceptors and are among the core proteins of chemosensory cascades. These enzymes have primarily been studied Escherichia coli and Salmonella typhimurium, which possess a single CheR involved in chemotaxis. Many other bacteria possess multiple cheR genes. Because the sequences of chemoreceptor signaling domains are highly conserved, it remains to be established with what degree of specificity CheR paralogues exert their activity. We report here a comparative analysis of the three CheR paralogues of Pseudomonas putida. Isothermal titration calorimetry studies show that these paralogues bind the product of the methylation reaction, S-adenosylhomocysteine, with much higher affinity (KD of 0.14–2.2 μm) than the substrate S-adenosylmethionine (KD of 22–43 μm), which indicates product feedback inhibition. Product binding was particularly tight for CheR2. Analytical ultracentrifugation experiments demonstrate that CheR2 is monomeric in the absence and presence of S-adenosylmethionine or S-adenosylhomocysteine. Methylation assays show that CheR2, but not the other paralogues, methylates the McpS and McpT chemotaxis receptors. The mutant in CheR2 was deficient in chemotaxis, whereas mutation of CheR1 and CheR3 had either no or little effect on chemotaxis. In contrast, biofilm formation of the CheR1 mutant was largely impaired but not affected in the other mutants. We conclude that CheR2 forms part of a chemotaxis pathway, and CheR1 forms part of a chemosensory route that controls biofilm formation. Data suggest that CheR methyltransferases act with high specificity on their cognate chemoreceptors.

ACS Style

Cristina Garcia Fontana; José Antonio Reyes-Darias; Francisco Munoz-Martinez; Carlos Alfonso; Bertrand Morel; Juan L. Ramos; Tino Krell. High Specificity in CheR Methyltransferase Function. Journal of Biological Chemistry 2013, 288, 18987 -18999.

AMA Style

Cristina Garcia Fontana, José Antonio Reyes-Darias, Francisco Munoz-Martinez, Carlos Alfonso, Bertrand Morel, Juan L. Ramos, Tino Krell. High Specificity in CheR Methyltransferase Function. Journal of Biological Chemistry. 2013; 288 (26):18987-18999.

Chicago/Turabian Style

Cristina Garcia Fontana; José Antonio Reyes-Darias; Francisco Munoz-Martinez; Carlos Alfonso; Bertrand Morel; Juan L. Ramos; Tino Krell. 2013. "High Specificity in CheR Methyltransferase Function." Journal of Biological Chemistry 288, no. 26: 18987-18999.

Journal article
Published: 22 May 2013 in Molecular Microbiology
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The paralogous receptors PctA, PctB and PctC of Pseudomonas aeruginosa were reported to mediate chemotaxis to amino acids, intermediates of amino acid metabolism and chlorinated hydrocarbons. We show that the recombinant ligand binding regions (LBRs) of PctA, PctB and PctC bind 17, 5 and 2 l-amino acids respectively. In addition, PctC-LBR recognized GABA but not any other structurally related compound. l-Gln, one of the three amino acids that is not recognized by PctA-LBR, was the most tightly binding ligand to PctB suggesting that PctB has evolved to mediate chemotaxis primarily towards l-Gln. Bacteria were efficiently attracted to l-Gln and GABA, but mutation of pctB and pctC, respectively, abolished chemoattraction. The physiological relevance of taxis towards GABA is proposed to reside in an interaction with plants. LBRs were predicted to adopt double PDC (PhoQ/DcuS/CitA) like structures and site-directed mutagenesis studies showed that ligands bind to the membrane-distal module. Analytical ultracentrifugation studies have shown that PctA-LBR and PctB-LBR are monomeric in the absence and presence of ligands, which is in contrast to the enterobacterial receptors that require sensor domain dimers for ligand recognition.

ACS Style

Miriam Rico-Jiménez; Francisco Munoz-Martinez; Cristina Garcia Fontana; Matilde Fernandez; Bertrand Morel; Alvaro Ortega; Juan L. Ramos; Tino Krell. Paralogous chemoreceptors mediate chemotaxis towards protein amino acids and the non-protein amino acid gamma-aminobutyrate (GABA). Molecular Microbiology 2013, 88, 1230 -1243.

AMA Style

Miriam Rico-Jiménez, Francisco Munoz-Martinez, Cristina Garcia Fontana, Matilde Fernandez, Bertrand Morel, Alvaro Ortega, Juan L. Ramos, Tino Krell. Paralogous chemoreceptors mediate chemotaxis towards protein amino acids and the non-protein amino acid gamma-aminobutyrate (GABA). Molecular Microbiology. 2013; 88 (6):1230-1243.

Chicago/Turabian Style

Miriam Rico-Jiménez; Francisco Munoz-Martinez; Cristina Garcia Fontana; Matilde Fernandez; Bertrand Morel; Alvaro Ortega; Juan L. Ramos; Tino Krell. 2013. "Paralogous chemoreceptors mediate chemotaxis towards protein amino acids and the non-protein amino acid gamma-aminobutyrate (GABA)." Molecular Microbiology 88, no. 6: 1230-1243.

Review article
Published: 19 November 2012 in Journal of Applied Microbiology
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A significant number of bacterial strains are able to use toxic aromatic hydrocarbons as carbon and energy sources. In a number of cases, the evolution of the corresponding degradation pathway was accompanied by the evolution of tactic behaviours either towards or away from these toxic carbon sources. Reports are reviewed which show that a chemoattraction to heterogeneously distributed aromatic pollutants increases the bioavailability of these compounds and their biodegradation efficiency. An extreme form of chemoattraction towards aromatic pollutants, termed ‘hyperchemotaxis’, was described for Pseudomonas putida DOT‐T1E, which is based on the action of the plasmid‐encoded McpT chemoreceptor. Cells with this phenotype were found of being able to approach and of establishing contact with undiluted crude oil samples. Although close McpT homologues are found on other degradation plasmids, the sequence of their ligand‐binding domains does not share significant similarity with that of NahY, the other characterized chemoreceptor for aromatic hydrocarbons. This may suggest the existence of at least two families of chemoreceptors for aromatic pollutants. The use of receptor chimers comprising the ligand‐binding region of McpT for biosensing purposes is discussed.

ACS Style

J. Lacal; J.A. Reyes-Darias; Cristina Garcia Fontana; Juan L. Ramos; T. Krell. Tactic responses to pollutants and their potential to increase biodegradation efficiency. Journal of Applied Microbiology 2012, 114, 923 -933.

AMA Style

J. Lacal, J.A. Reyes-Darias, Cristina Garcia Fontana, Juan L. Ramos, T. Krell. Tactic responses to pollutants and their potential to increase biodegradation efficiency. Journal of Applied Microbiology. 2012; 114 (4):923-933.

Chicago/Turabian Style

J. Lacal; J.A. Reyes-Darias; Cristina Garcia Fontana; Juan L. Ramos; T. Krell. 2012. "Tactic responses to pollutants and their potential to increase biodegradation efficiency." Journal of Applied Microbiology 114, no. 4: 923-933.