Ginkgo biloba is a traditional Chinese medicinal plant which has potent insecticidal activity against brown planthopper. The MeOH extract was tested in the acetylcholinesterase (AChE) inhibitory assay with IC50 values of 252.1 μg/mL. Two ginkgolides and thirteen flavonoids were isolated from the leaves of Ginkgo biloba. Their structures were established on the basis of spectroscopic data interpretation. It revealed that the 13 isolated flavonoids were found to inhibit AChE with IC50 values ranging from 57.8 to 133.1 μg/mL in the inhibitory assay. AChE was inhibited dose dependently by all tested flavonoids, and compound 6 displayed the highest inhibitory effect against AChE with IC50 values of 57.8 μg/mL.
Three active glycosides that afford protection to red blood cell membrane to resist hemolysis induced by a peroxyl radical initiator, 2,2′-azo-bis-(2-amidinopropane) dihydrochloride (AAPH) were isolated from the MeOH extract of Ligustrum sinense. The compounds are 10-hydroxyl-oleuropein (1), 3-O-α-lrhamno-pyranosyl-kaempferol-7-O-β-Dglucopyranoside (4), and 8′-α-hydroxyl-lariciresinol-4′-O-β-Dglucopyranoside (6). The structures of these glycosides were determined by 1D and 2D NMR spectral analysis.
Two new compounds named fischeacid (1) and fischexanthone (2), together with eight known compounds, were obtained from the culture of a marine-derived fungus Neosartorya fischeri strain 1008F1. The structures of the new compounds were elucidated based on the spectroscopic data. Bioassays indicated that AGI-B4 (5) and 3,4-dihydroxybenzoic acid (10) showed potent inhibitory effect on the replication of tobacco mosaic virus, and AGI-B4 also possessed an inhibition of the cell proliferation of human gastric cancer cell line SGC-7901 and hepatic cancer cells BEL-7404.
A new adenosyl-alkaloid, ostrerine A (1), has been isolated along with an amino acid, tryptophan (2) and a ribonucleoside, 2′-deoxythymidine (3) from the Quanzhou marine mollusk, Ostrea rivularis, and the structures were elucidated by 1D and 2D NMR experiments, including 1H-1H COSY, TOCSY, NOESY, HMQC, and HMBC methods.
New water-soluble phenylpropanoid constituents, ficuscarpanoside A, guaiacylglycerol 9-O-β-D-glucopyranoside, and erythro-guaiacylglycerol 9-O-β-D-glucopyranoside, along with known guaiacylglycerol, erythro-guaiacylglycerol, 4-methoxy guaiacylglycerol 7-O-β-D-glucopyranoside, and 3-(4-hydroxy-3-methoxy phenyl) propan-1,2-diol, have been isolated from the aerial roots of Ficus microcarpa. Their structures were elucidated on the basis of 1D and 2D NMR experiments.
Two new phenolic water-soluble constituents, involcranoside A (1) and involcranoside B (2) have been isolated along with five known phenolic compounds: 3,4-dimethoxyphenyl-O-β-D-gluco-pyranoside (3), picein (4), and 1,4-dihydroxy-3-methoxy-phenyl-4-O-β-D-glucopyranoside (5), leonuriside A (6) and 4-hydroxy-3-methoxybenzoic acid (7) from the branch bark of Davidia involucrata. Identification of their structures was achieved by 1D and 2D NMR experiments, including 1H-1H COSY, NOESY, HMQC and HMBC methods and FAB mass spectral data.