The particle size (PS) and encapsulation efficiency (EE%) of drug-loaded nanoparticles (NPs) may inhibit their cellular uptake and lead to possible leakage of the drug into the systemic circulation at the tumor site. In this work, ultra-high paclitaxel-loaded poly(lactide-co-glycolide) NPs (PTX-PLGA-NPs) with ultra-small sizes were prepared and optimized by adopting the principles of quality by design (QbD) approach. The optimized PTX-PLGA-NPs showed ultra-small spherical particles of about 53 nm with EE% exceeding 90%, a relatively low polydispersity index (PDI) of 0.221, an effective surface charge of −10.1 mV, and a 10-fold increase in the in vitro drug release over 72 h relative to free drug. The cellular viability of pharynx carcinoma cells decreased by almost 50% in 24 h following treatment with optimized PTX-PLGA-NPs, compared to only 20% from the free drug. The intracellular uptake of PTX-PLGA-NPs was highly favored, and the antitumor activity of PTX was remarkably improved with a reduction in its half maximal inhibitory concentration (IC₅₀), by almost 50% relative to free drug solution. These results suggest that the optimal critical formulation parameters, guided by QbD principles, could produce PLGA-NPs with remarkably high EE% and ultra-small PS, resulting in enhanced cellular uptake and efficacy of PTX.

The food industry represents a vast environmental burden to our planet. Most animal products are known to have greater environmental impacts than alternative plant-based sources of nutrition. One of the most consumed animal products are eggs, represented in most dietary habits both as a primary ingredient and processed. In the European Union (EU), eggs for consumption can be produced in four different laying systems: enriched cages, barns, free-range, and organic. In past years, discussions about the ethical perspective have been ongoing among the wider public, industry and academia. However, the essential comparison of environmental impacts of the laying systems has been missing in our region. Life Cycle Assessment (LCA) is an excellent tool for comparing environmental impacts of various scenarios. Therefore, we performed a LCA of several egg-producing subjects in the Czech Republic, representative of all four laying systems. In addition, these regulated laying systems were compared to a community garden system. Our results suggest feed conversion ratio (FCR), feed composition, and manure management to be the most important factors influencing the total environmental impacts of eggs. Moreover, environmental benefits linked to outdoor access or using organic feed over conventional were observed in our study.