Phosphorus accumulation in soil leads to soil quality degradation and yield reduction. Therefore, effective phosphorus management is essential for maintaining a sustainable agroecosystem and ensuring crop productivity. This study aimed to evaluate the effects of various organic materials on phosphate availability in phosphorus-accumulated soil, identify suitable organic materials, and determine optimal application rates for solubilizing accumulated phosphate. The materials tested including organic acid, peat moss, zeolite, sulfur, hairy vetch, rice straw, wheat bran, expeller cake, and DTPA (a chelating agent). The treatment rates were applied on a weight/weight (w/w) basis, with DTPA and distilled water serving as the controls. Soil pH, available phosphate, water-soluble phosphate, and phosphatase activity were analyzed at 0, 7, 14, and 30 days through incubation experiments. The results indicated that sulfur, expeller cake, DTPA and hairy vetch effectively reduced soil pH. Available phosphate increased by 25% and 17% compared to the control with sulfur and expeller cake, respectively. Water-soluble phosphate increased by 21% to 75% compared to the control with sulfur, rice straw (1%), zeolite, expeller cake (2%), and wheat bran (2%). Acid phosphatase activity was enhanced by organic acid (0.5%, 1%), zeolite (1%), rice straw (2%), wheat bran, and hairy vetch (0.5%, 1%, 2%). This study demonstrated that organic materials have phosphate solubilization effects comparable to DTPA.