The aim of the study was to evaluate 11 cultivars of blue honeysuckle (Lonicera caerulea L.) for bioactive compounds, antioxidant capacity, and the antibacterial activity of berries. Total phenolic contents (TPCs) and total anthocyanin contents (TACs) were established by using ethanolic extracts. For contents of organic acids and saccharides, aqueous extracts were used, and vitamin C was determined by using oxalic acid solution. DPPH^• radical scavenging capacity was evaluated by using ethanolic extracts; antibacterial activity was assessed by using both ethanolic and aqueous extracts. The TPC varied from 364.02 ± 0.41 mg/100 g in ‘Vostorg’ to 784.5 ± 0.3 mg/100 g in ‘Obilnaja’, and TAC ranged from 277.8 ± 1.1 mg/100 g in ‘Čelnočnaja’ to 394.1 ± 8.4 mg/100 g in ‘Nimfa’. Anthocyanins comprised 53.8% of total phenolic contents on average. Among organic acids, citric acid was predominant, averaging 769.41 ± 5.34 mg/100 g, with malic and quinic acids amounting to 289.90 ± 2.64 and 45.00 ± 0.37 mg/100 g on average, respectively. Contents of vitamin C were 34.26 ± 0.25 mg/100 g on average. Organic acids were most effective in the inhibition of both Gram-positive and Gram-negative bacteria tested. In conclusion, berries of L. caerulea are beneficial not only for fresh consumption, but also as a raw material or ingredients of foods with high health-promoting value.

Norway spruce (Picea abies (L.) H. Karst.) is one of the most important commercial tree species distributed naturally in the Boreal and subalpine forest zone of Europe. All parts of spruce trees, including needles, accumulate essential oils with a variety of chemical properties and ecological functions, such as modulating plant–insect communication. Annual needle samples from 15 trees (five from each of three habitats) of 15–17 years old were assayed for essential oils and their major compounds, including α-pinene, β-pinene, (1S)-(−)-α-pinene, and (1R)-(+)-α-pinene across a growing season. Results showed strong positive correlation between percentages of α- and β-pinene isomers (r = 0.69, p < 0.05) and between pinene isomers and essential oils: α-pinene correlated with essential oil stronger (r = 0.62, p < 0.05) than β-pinene (r = 0.33, p < 0.05). Correlation analyses performed with some weather conditions, including average monthly temperature, growing sum of effective temperatures over 5 °C, duration of sunshine, accumulated precipitation, relative humidity, and pressure, showed that temperature is the most important weather condition related to pinene dynamics: negative correlations of moderate strength were established between percentages of α- and β- pinenes and average monthly temperatures (r = −0.36, p < 0.01, n = 75 and r = −0.33, p < 0.01, n = 75, respectively). Out of pinene enantiomers, only (1S)-(−)-α-pinene showed some negative correlation with monthly temperature (r = −0.26, p < 0.05, n = 75). Different patterns of essential oil and pinene dynamics during growing season within separate habitats suggested that some genetic variables of Picea abies might be involved.

Thirty-one raspberry (Rubus idaeus L.) plants were collected in different natural habitats of Lithuania located in 17 districts and replanted in the experimental field. The fruits from different accessions were extracted with ethanol and the extracts were tested for their antioxidant activity (AA) by using ABTS·+ radical cation decolourisation and DPPH·radical scavenging methods. All tested raspberry fruit extracts were antioxidatively active; their radical scavenging capacity at the applied concentrations varied from 52.9 to 92.6% in DPPH·reaction system and from 52.5 to 97.8% in ABTS·+system. The total amount of phenolic compounds in fruits varied from 5.6 to 13.7 mg of gallic acid equivalents in 1 g of plant extract. However, strong correlation between the radical scavenging capacity and the total amount of phenolic compounds in the fruit extracts was not observed, which indicates remarkable differences in the composition and antioxidant power of phenolic compounds present in the fruits harvested from raspberry accessions collected from different natural habitats. Most likely, environmental conditions and genetic factors should play an important role for the accumulation of active compounds in raspberry fruits.\u

Tree genetic diversity is among the most important factors determining the sustainability of forest ecosystems. The main aim of the present study was to track possible changes in genetic diversity of regenerating populations of Norway spruce (Picea abies (L.) H. Karst) and Scots pine (Pinus sylvestris L.) in areas subjected either to a natural disturbance (windthrows and subsequent clear-cutting of the affected spruce stand) or to a changed land-use legacy (pine regeneration on abandoned agricultural land) with the aim of testing whether the new forest generation retains the genetic diversity of the putative maternal stand. Eight highly polymorphic microsatellite loci were used to reveal the genetic diversity and its spatial distribution in the studied tree populations. Self-regenerating juveniles of Norway spruce and Scots pine were spatially random and as genetically diverse as in the putative maternal populations. Genetic differentiation between putatively maternal trees and regenerating juveniles was low for both species. A high genetic diversity and random spatial genetic structure revealed in the regenerating populations provides a basis for the formation of evolutionary and ecologically sound stands able to adapt to ever-changing climatic conditions. Information on the genetic dynamics of the studied natural populations of long-lived coniferous tree species may be important for evaluating possible changes in genetic diversity at a local scale following forest ecosystem disturbances and changes in land-use legacies.

Twenty six seed (genetic) sites were established for long-term in-situ conservation of medicinal and aromatic plant (MAP) genetic resources in Lithuania over the period 2006–2017. The sites vary in size from 0.4 to 38.0 ha with an average of 7.2 ha. Most of the sites (73%) occur in the existing protected areas. They represent 10 of 22 physical geographical areas of Lithuania and accommodate 120 priority MAP species, which is about 89% of the national priority list. Prioritization of MAP species was based on the monographs of European Pharmacopoeia, European Medicines Agency, World Health Organization as well as German Phytotherapeutic Monographs and other regional and national medicinal plant references. A concept was followed of the dual function and perception of food as medicine, and, vice versa, an approach based on a co-evolutionary relationship between human food and medicine. It was established that only 46 priority species, or 34% of the national priority list, were represented by five and more populations, which is considered as minimum for long-term in-situ conservation. The future conservation planning efforts should focus on at least 15 unrepresented species, 49 species represented at 1–2 sites each and 25 species represented at 3–4 sites each. The MAP site network should expand by covering preferably the remaining 12 physical geographical areas of the country.