Carboxylated single‐walled carbon nanotubes (SwCNT_COOH) were used as a support for the covalent immobilization of phenylalanine ammonia‐lyase (PAL) from parsley by two different methods. The nanostructured biocatalysts (SwCNT_COOH‐PAL^I and SwCNT_COOH‐PAL^II) with low diffusional limitation were tested in the batch‐mode kinetic resolution of racemic 2‐amino‐3‐(thiophen‐2‐yl)propanoic acid (1) to yield a mixture of (R)‐1 and (E)‐3‐(thiophen‐2‐yl)acrylic acid (2) and in ammonia addition to 2 to yield enantiopure (S)‐1. SwCNT_COOH‐PAL^II was a stable biocatalyst (>90 % of the original activity remained after six cycles with 1 and after three cycles in 6 M NH₃ with 2). The study of ammonia addition to 2 in a continuous‐flow microreactor filled with SwCNT_COOH‐PAL^II (2 M NH₃, pH 10.0, 15 bar) between 30–80 °C indicated no significant loss of activity over 72 h up to 60 °C. SwCNT_COOH‐PAL^II in the continuous‐flow system at 30 °C was more productive (specific reaction rate, r_flow=2.39 μmol min⁻¹ g⁻¹) than in the batch reaction (r_batch=1.34 μmol min⁻¹ g⁻¹).