This page has only limited features, please log in for full access.

Prof. Dr. Dusan Palic
Chair for Fish Diseases and Fisheries Biology, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, Munich, Germany

Basic Info


Research Keywords & Expertise

0 Vaccinology
0 Immunomodulators
0 immunotoxicity
0 Aquatic veterinary medicine
0 Innate immunology of aquatic animals

Fingerprints

immunotoxicity

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 10 March 2021 in Viruses
Reads 0
Downloads 0

Novirhabdoviruses cause large epizootics and economic losses of farmed trout. In this study, we surveyed Viral hemorrhagic septicemia virus and Infectious hematopoietic and necrosis virus (VHSV and IHNV) through both monitoring and investigation of clinical outbreaks reported by farmers in the regions with major rainbow trout production in Iran from 2015 to 2019. RT-PCR assays of the kidney samples and cell culture (EPC/FHM cells) samples confirmed the presence of the viruses, with 9 VHSV and 4 IHNV isolates, in both endemic and new areas of Iran. Sequence analysis of the G gene revealed that VHSV isolates belonged to genogroup Ia, and IHNV isolates were clustered into genogroup E, both typical for isolates from European countries. A haplotype analysis based on non-homologous amino acids of the G gene supports the emergence of two lineages of IHNV from clade 1 (E-1), as well as VHSV clade 2 (Ia-2) of the European genogroups, confirming that VHSV and IHNV isolates in Iran, have originated from Europe possibly via imported eggs.

ACS Style

Sohrab Ahmadivand; Dušan Palić; Manfred Weidmann. Molecular Epidemiology of Novirhabdoviruses Emerging in Iranian Trout Farms. Viruses 2021, 13, 448 .

AMA Style

Sohrab Ahmadivand, Dušan Palić, Manfred Weidmann. Molecular Epidemiology of Novirhabdoviruses Emerging in Iranian Trout Farms. Viruses. 2021; 13 (3):448.

Chicago/Turabian Style

Sohrab Ahmadivand; Dušan Palić; Manfred Weidmann. 2021. "Molecular Epidemiology of Novirhabdoviruses Emerging in Iranian Trout Farms." Viruses 13, no. 3: 448.

Journal article
Published: 24 February 2021 in Aquaculture Reports
Reads 0
Downloads 0

Increased sales of freshwater catfish fillet in global markets is causing high demand for quality fingerlings to be used in aquaculture production. Due to limited freshwater catfish broodstock availability, the gap between fingerling supply and demand drives the continuous need for improvement of production chain, including increased fertility and fecundity of broodstock. Recent technological advances support the possibility to increase hybrid catfish (Pangasius larnaudii x Pangasianodon hypophthalmus) broodstock reproductive parameters by adding small amounts of freshwater fish oil (FFO) to their diet. Catfish broodstock were fed with three different levels of FFO feed supplementation (0%, 1%, and 2% FFO) during the spawning (April-August) and post-spawning (September-December) season. Significant differences in weight gain and average daily growth were observed in fish fed with 1% FFO supplemented diet. Biomarkers of reproductive status in females (17 β-estradiol /E2; 1577 ± 5.26 pg/mL−1) and males (testosterone /T; 3.28 ± 0.41 ng/mL−1) fed with FFO supplements were significantly higher than the control (113.12 ± 43.33 pg/mL−1 and 2.56 ± 0.95 ng/mL−1), respectively). The oocytes and spermatocytes from fish fed with 1% and 2% FFO were in higher histological stages of maturity compared to control. Freshwater fish oil supplementation in fish feed during spawning season supported increased growth and enhanced reproduction indicators of hybrid catfish broodstock in aquaculture.

ACS Style

Supaporn Sattang; Doungporn Amornlerdpison; Sudaporn Tongsiri; Dušan Palić; Kriangsak Mengumphan. Effect of freshwater fish oil feed supplementation on the reproductive condition and production parameters of hybrid catfish (Pangasius larnaudii x Pangasianodon hypophthalmus, Sauvage, 1878) broodstock. Aquaculture Reports 2021, 20, 100598 .

AMA Style

Supaporn Sattang, Doungporn Amornlerdpison, Sudaporn Tongsiri, Dušan Palić, Kriangsak Mengumphan. Effect of freshwater fish oil feed supplementation on the reproductive condition and production parameters of hybrid catfish (Pangasius larnaudii x Pangasianodon hypophthalmus, Sauvage, 1878) broodstock. Aquaculture Reports. 2021; 20 ():100598.

Chicago/Turabian Style

Supaporn Sattang; Doungporn Amornlerdpison; Sudaporn Tongsiri; Dušan Palić; Kriangsak Mengumphan. 2021. "Effect of freshwater fish oil feed supplementation on the reproductive condition and production parameters of hybrid catfish (Pangasius larnaudii x Pangasianodon hypophthalmus, Sauvage, 1878) broodstock." Aquaculture Reports 20, no. : 100598.

Journal article
Published: 23 September 2020 in Pathogens
Reads 0
Downloads 0

From May to October 2019, multiple mass mortality events (MMEs) of Pinna nobilis were observed along Croatian coastline starting from the south-east and rapidly progressing in north-western direction. Time dynamics of the MMEs closely followed general speed and direction patterns of surface sea-currents, advancing approximately 350 km in less than 3 months. Surveillance, clinical evaluation, and sample collection were performed on multiple sites with various degrees of mortality rates. Moribund P. nobilis individuals were collected and subjected to pathological, molecular, and microscopical investigation. Affected animals were positive for Mycobacterium in 70% of the individuals, and Haplosporidium pinnae was present in 58% of the cases. Observed pathological lesions were most severe where concurrent presence of both pathogens was confirmed (in 45.8% of moribund individuals). Moderate to strong lesions were observed in animals positive for Mycobacterium only (25% of cases), and lesions were absent or minor to moderate when only H. pinnae was confirmed (16% of cases). Considering the rapid and severe spread of the MMEs, the areas less exposed to major sea currents appeared to be at lower risk of pathogen transmission. Surveillance activities along the Croatian coastline identified several P. nobilis populations in such “lower risk” areas without apparent mortality or clinical symptoms. Such areas are of particular interest as source of potentially healthy individuals to support active recovery actions.

ACS Style

Tomislav Šarić; Ivan Župan; Serena Aceto; Grazia Villari; Dušan Palić; Gionata De Vico; Francesca Carella. Epidemiology of Noble Pen Shell (Pinna nobilis L. 1758) Mass Mortality Events in Adriatic Sea Is Characterised with Rapid Spreading and Acute Disease Progression. Pathogens 2020, 9, 776 .

AMA Style

Tomislav Šarić, Ivan Župan, Serena Aceto, Grazia Villari, Dušan Palić, Gionata De Vico, Francesca Carella. Epidemiology of Noble Pen Shell (Pinna nobilis L. 1758) Mass Mortality Events in Adriatic Sea Is Characterised with Rapid Spreading and Acute Disease Progression. Pathogens. 2020; 9 (10):776.

Chicago/Turabian Style

Tomislav Šarić; Ivan Župan; Serena Aceto; Grazia Villari; Dušan Palić; Gionata De Vico; Francesca Carella. 2020. "Epidemiology of Noble Pen Shell (Pinna nobilis L. 1758) Mass Mortality Events in Adriatic Sea Is Characterised with Rapid Spreading and Acute Disease Progression." Pathogens 9, no. 10: 776.

Review article
Published: 17 July 2020 in Redox Biology
Reads 0
Downloads 0

Microplastics (MPs) and nanoplastics (NPs) have attracted considerable attention in the recent years as potential threats to the ecosystem and public health. This review summarizes current knowledge of pathological events triggered by micro- and nano-plastics (MP/NPs) with focus on oxidative damages at different levels of biological complexity (molecular, cellular, tissue, organ, individual and population). Based on published information, we matched the apical toxicity endpoints induced by MP/NPs with key event (KE) or adverse outcomes (AO) and categorized them according to the Adverse Outcome Pathway (AOP) online knowledgebase. We used existing AOPs and applied them to highlight formal mechanistic links between identified KEs and AOs in two possible scenarios: first from ecological, and second from public health perspective. Ecological perspective AOP based literature analysis revealed that MP/NPs share formation of reactive oxygen species as their molecular initiating event, leading to adverse outcomes such as growth inhibition and behavior alteration through oxidative stress cascades and inflammatory responses. Application of AOP on literature data related to public health perspective of MP/NPs showed that oxidative stress and its responding pathways, including inflammatory responses, could play the role of key events. However insufficient information prevented precise definitions of AOPs at this level. To overcome this knowledge gap, further mammalian model and epidemiological studies are necessary to support development and construction of detailed AOPs with public health focus.

ACS Style

Moyan Hu; Dušan Palić. Micro- and nano-plastics activation of oxidative and inflammatory adverse outcome pathways. Redox Biology 2020, 37, 101620 .

AMA Style

Moyan Hu, Dušan Palić. Micro- and nano-plastics activation of oxidative and inflammatory adverse outcome pathways. Redox Biology. 2020; 37 ():101620.

Chicago/Turabian Style

Moyan Hu; Dušan Palić. 2020. "Micro- and nano-plastics activation of oxidative and inflammatory adverse outcome pathways." Redox Biology 37, no. : 101620.

Journal article
Published: 03 June 2020 in Toxicology Reports
Reads 0
Downloads 0

The release of nanoparticles into the environment can interfere with the health of the exposed organisms. MicroRNAs have been suggested as potential toxicology biomarkers. The expression of potential zebrafish nano-toxicity biomarker miRNAs in our previous study was validated in THP-1 human monocytic cell line after exposure to polystyrene (PSNPs) and ARS labeled Titanium dioxide nanoparticles (nano-TiO2-ARS). miRNAs expression post exposure to PLGA nanoparticles and E. coli BioParticles was used to exclude potential activation and engagement of miRNAs through phagocytosis or pro-inflammatory specific responses. miR-155-5p showed the highest potential to be used as biomarker for PSNPs and nano-TiO2-ARS induced toxicity. To determine effects of PSNPs and nano-TiO2-ARS on genotoxicity, time and dose dependent DNA damage profile was established. Severe DNA damage was triggered by both nanoparticles, and expression of DNA damage repairing genes was elevated post nano-TiO2-ARS, but not post PSNPs exposure, questioning the utility of the comet assay as universal assessment tool for genotoxicity induced by nanoparticles in general. Transfection of miR-155-5p mimic influenced the expression of miR-155-5p related, DNA damage responsible genes post both nano-TiO2-ARS and PSNPs exposure. Transfection results suggest significant involvement of miR-155-5p in gene repair mechanisms triggered by adverse effects of PSNPs and nano-TiO2-ARS on monocytes.

ACS Style

Moyan Hu; Dušan Palić. Role of MicroRNAs in regulation of DNA damage in monocytes exposed to polystyrene and TiO2 nanoparticles. Toxicology Reports 2020, 7, 743 -751.

AMA Style

Moyan Hu, Dušan Palić. Role of MicroRNAs in regulation of DNA damage in monocytes exposed to polystyrene and TiO2 nanoparticles. Toxicology Reports. 2020; 7 ():743-751.

Chicago/Turabian Style

Moyan Hu; Dušan Palić. 2020. "Role of MicroRNAs in regulation of DNA damage in monocytes exposed to polystyrene and TiO2 nanoparticles." Toxicology Reports 7, no. : 743-751.

Review article
Published: 24 May 2019 in Toxicology in Vitro
Reads 0
Downloads 0

The release of nanoparticles to the environment can affect health of the exposed organisms. MicroRNAs have been suggested as potential toxicology biomarkers, however the information about use of microRNA in aquatic organisms exposed to nanoparticles (NP) is limited. In silico analysis from publicly available gene expression data was performed. Data selection for the analysis was based on reported biological and pathological outcomes of NP induced toxicity in zebrafish. After identifying relevant genes, we constructed six miRNA-mRNA regulatory networks involved in nanoparticle induced toxicological responses in zebrafish. Based on our prediction and selection criteria we selected six miRNAs that overlapped in constructed networks with remarkable prediction score, and were validated by previous mammalian and zebrafish microRNA profiling studies: dre-miR-124, −144, −148, −155, −19a, −223. The results of this in silico analysis indicate that several highly conserved miRNAs likely have a regulatory role of organismal responses to nanoparticles, and can possibly be used as biomarkers of nanotoxicity in studies using zebrafish as model organism One health approaches.

ACS Style

Moyan Hu; Boris Jovanović; Dušan Palić. In silico prediction of MicroRNA role in regulation of Zebrafish (Danio rerio) responses to nanoparticle exposure. Toxicology in Vitro 2019, 60, 187 -202.

AMA Style

Moyan Hu, Boris Jovanović, Dušan Palić. In silico prediction of MicroRNA role in regulation of Zebrafish (Danio rerio) responses to nanoparticle exposure. Toxicology in Vitro. 2019; 60 ():187-202.

Chicago/Turabian Style

Moyan Hu; Boris Jovanović; Dušan Palić. 2019. "In silico prediction of MicroRNA role in regulation of Zebrafish (Danio rerio) responses to nanoparticle exposure." Toxicology in Vitro 60, no. : 187-202.

Journal article
Published: 18 July 2016 in Environmental Toxicology and Chemistry
Reads 0
Downloads 0

Water pollution with large and small-scale plastic litter is an area of growing concern. Macro plastic litter is a well-known threat to aquatic wildlife; however, effects of micro and nano-sized plastic particles on the health of organisms are not well understood. Small scale plastic particles can easily be ingested by various aquatic organisms and potentially interfere with their immune system, therefore we used a freshwater fish species as a model organism for nanoplastic exposure. Characterization of polystyrene and polycarbonate nanoplastic (PS: 41.0 nm, PC: 158.7 nm) particles (PSNP and PCNP, respectively) in plasma was performed, and effects of PSNP and PCNP on the innate immune system of fathead minnow, were investigated. In vitro effects of PSNP and PCNP on neutrophil function were determined using a battery of neutrophil function assays. Exposure of neutrophils to PSNP or PCNP caused significant increase in degranulation of primary granules and neutrophil extracellular traps (NETs) release compared to a non-treated control, while oxidative burst was less affected, This study outlines the stress response of the cellular component of fish innate immune system to polystyrene and polycarbonate nanoparticles/aggregates and indicates their potential to interfere with disease resistance in fish populations. This article is protected by copyright. All rights reserved

ACS Style

Anne-Catherine Greven; Teresa Merk; Filiz Karagöz; Kristin Mohr; Markus Klapper; Boris Jovanović; Dušan Palić. Polycarbonate and polystyrene nanoplastic particles act as stressors to the innate immune system of fathead minnow (Pimephales promelas). Environmental Toxicology and Chemistry 2016, 35, 3093 -3100.

AMA Style

Anne-Catherine Greven, Teresa Merk, Filiz Karagöz, Kristin Mohr, Markus Klapper, Boris Jovanović, Dušan Palić. Polycarbonate and polystyrene nanoplastic particles act as stressors to the innate immune system of fathead minnow (Pimephales promelas). Environmental Toxicology and Chemistry. 2016; 35 (12):3093-3100.

Chicago/Turabian Style

Anne-Catherine Greven; Teresa Merk; Filiz Karagöz; Kristin Mohr; Markus Klapper; Boris Jovanović; Dušan Palić. 2016. "Polycarbonate and polystyrene nanoplastic particles act as stressors to the innate immune system of fathead minnow (Pimephales promelas)." Environmental Toxicology and Chemistry 35, no. 12: 3093-3100.

Journal article
Published: 01 August 2015 in Environmental Pollution
Reads 0
Downloads 0

Nano-TiO2 is immunotoxic to fish and reduces the bactericidal function of fish neutrophils. Here, fathead minnows (Pimephales promelas) were exposed to low and high environmentally relevant concentration of nano-TiO2 (2 ng g(-1) and 10 μg g(-1) body weight, respectively), and were challenged with common fish bacterial pathogens, Aeromonas hydrophila or Edwardsiella ictaluri. Pre-exposure to nano-TiO2 significantly increased fish mortality during bacterial challenge. Nano-TiO2 concentrated in the kidney and spleen. Phagocytosis assay demonstrated that nano-TiO2 has the ability to diminish neutrophil phagocytosis of A. hydrophila. Fish injected with TiO2 nanoparticles displayed significant histopathology when compared to control fish. The interplay between nanoparticle exposure, immune system, histopathology, and infectious disease pathogenesis in any animal model has not been described before. By modulating fish immune responses and interfering with resistance to bacterial pathogens, manufactured nano-TiO2 has the potential to affect fish survival in a disease outbreak.

ACS Style

Boris Jovanović; Elizabeth M. Whitley; Kayoko Kimura; Adam Crumpton; Dušan Palić. Titanium dioxide nanoparticles enhance mortality of fish exposed to bacterial pathogens. Environmental Pollution 2015, 203, 153 -164.

AMA Style

Boris Jovanović, Elizabeth M. Whitley, Kayoko Kimura, Adam Crumpton, Dušan Palić. Titanium dioxide nanoparticles enhance mortality of fish exposed to bacterial pathogens. Environmental Pollution. 2015; 203 ():153-164.

Chicago/Turabian Style

Boris Jovanović; Elizabeth M. Whitley; Kayoko Kimura; Adam Crumpton; Dušan Palić. 2015. "Titanium dioxide nanoparticles enhance mortality of fish exposed to bacterial pathogens." Environmental Pollution 203, no. : 153-164.

Review
Published: 01 August 2012 in Aquatic Toxicology
Reads 0
Downloads 0

The rapid increase in use of nanotechnology products is increasing the presence of metal, metal-oxide and carbon-based nanoparticles in the aquatic environment. These non-functionalized engineered nanoparticles can interact with the immune system of fish and invertebrates, and tip the ecological balance of population sustainability. Most nanoparticle types present in the aquatic environment, such as titanium dioxide, do not exhibit or have very low direct toxicity, but instead display silent or concealed sub-lethal effects on the immune system with serious implications. There is a gap in current available information regarding the immunotoxic potential of engineered nanoparticles toward aquatic organisms. Therefore, there is a critical need to provide the first comprehensive review of the effects of engineered non-functionalized nanoparticles on the immune system of aquatic animals, address the major gaps in current existing information, and recommend the future focus of research. This manuscript identifies cell mediated immunity and the phagocytic cells as the primary target of nanoparticle immunotoxicity. The immunotoxicity is primarily govern by lysosomal destabilization, frustrated phagocytosis, and change in function of the phagocytic cells, which decrease the ability of animals to defend themselves against pathogens and infectious diseases. Humoral immune system is a lesser target of direct immunotoxicity, but plays a critical role in dissemination of the nanoparticles through the body and their presentation to the phagocytic cells. The external innate immunity and the acquired immunity have not been connected with overly important and direct immunotoxic effects, but instead a big gap in current targeted research has been acknowledged.

ACS Style

Boris Jovanović; Dušan Palić. Immunotoxicology of non-functionalized engineered nanoparticles in aquatic organisms with special emphasis on fish—Review of current knowledge, gap identification, and call for further research. Aquatic Toxicology 2012, 118-119, 141 -151.

AMA Style

Boris Jovanović, Dušan Palić. Immunotoxicology of non-functionalized engineered nanoparticles in aquatic organisms with special emphasis on fish—Review of current knowledge, gap identification, and call for further research. Aquatic Toxicology. 2012; 118-119 ():141-151.

Chicago/Turabian Style

Boris Jovanović; Dušan Palić. 2012. "Immunotoxicology of non-functionalized engineered nanoparticles in aquatic organisms with special emphasis on fish—Review of current knowledge, gap identification, and call for further research." Aquatic Toxicology 118-119, no. : 141-151.

Journal article
Published: 01 May 2011 in Ecotoxicology and Environmental Safety
Reads 0
Downloads 0

Effects of nanosized (<100 nm) titanium dioxide (TiO(2)) particles on fish neutrophils and immune gene expression was investigated using the fathead minnow (Pimpehales promelas). Expanded use of TiO(2) in the cosmetic industry has increased the potential exposure risk to aquatic ecosystems and human health. Effects of nano-TiO(2) on neutrophil function of the fathead minnow was investigated using oxidative burst, neutrophil extracellular traps (NETs) release and degranulation of primary granules. The innate immune gene expression was determined with quantitative PCR (qPCR). Application of 0.1 μg mL(-1) of nano-TiO(2) in vitro stimulated oxidative burst and NET release. Intraperitoneal injection of 10 μg g(-1) of nano-TiO(2) caused a significant decrease in oxidative burst, NETs release and degranulation (21%; 11%; and 30%, decrease, respectively). Fish exposed to nano-TiO(2) for 48 h in vivo had significantly increased expression of interleukin 11, macrophage stimulating factor 1, and neutrophil cytosolic factor 2 (4; 2.5; and 2 fold increase, respectively). Nano-TiO(2) has potential to interfere with the evolutionary conserved innate immune system responses, as evidenced with observed changes in gene expression and neutrophil function. This finding encourages the use of fish models in the studies of nanoparticle immunotoxicity. The lowest significant response concentration studied in vitro is four times greater than the estimated environmental concentration for TiO(2) (0.025 μg mL(-1)) causing concern about potential impact of nano-TiO(2) on aquatic animals and ecosystems.

ACS Style

Boris Jovanović; Lora Anastasova; Eric W. Rowe; Yanjie Zhang; Aaron R. Clapp; Dušan Palić. Effects of nanosized titanium dioxide on innate immune system of fathead minnow (Pimephales promelas Rafinesque, 1820). Ecotoxicology and Environmental Safety 2011, 74, 675 -683.

AMA Style

Boris Jovanović, Lora Anastasova, Eric W. Rowe, Yanjie Zhang, Aaron R. Clapp, Dušan Palić. Effects of nanosized titanium dioxide on innate immune system of fathead minnow (Pimephales promelas Rafinesque, 1820). Ecotoxicology and Environmental Safety. 2011; 74 (4):675-683.

Chicago/Turabian Style

Boris Jovanović; Lora Anastasova; Eric W. Rowe; Yanjie Zhang; Aaron R. Clapp; Dušan Palić. 2011. "Effects of nanosized titanium dioxide on innate immune system of fathead minnow (Pimephales promelas Rafinesque, 1820)." Ecotoxicology and Environmental Safety 74, no. 4: 675-683.

Journal article
Published: 25 January 2011 in Aquatic Toxicology
Reads 0
Downloads 0

Hydroxylated fullerenes act as potent inhibitors of cytochrome P450-dependent monooxygenases, and are reported to be very strong antioxidants quenching reactive oxygen species (ROS) production. Effects of nanosized hydroxylated fullerenes on fish neutrophil function and immune gene transcription was investigated using fathead minnow (Pimephales promelas). Neutrophil function assays were used to determine the effects of fullerene exposure in vitro and in vivo on oxidative burst, degranulation and extracellular trap (NETs) release, and the innate immune gene trascription was determined with quantitative PCR (qPCR). Application of fullerenes (0.2–200 μg mL−1in vitro) caused concentration dependent inhibition of oxidative burst and suppressed the release of NETs and degranulation of primary granules (up to 70, 40, and 50% reduction in activity compared to non-treated control, respectively). Transcription of interleukin 11 and myeloperoxidase genes was significantly increased and transcription of elastase 2 gene was significantly decreased in fish exposed to hydroxylated fullerenes for 48 h in vivo (12 and 3 fold increase, and 5 fold decrease, respectively). Observed changes in gene transcription and neutrophil function indicate potential for hydroxylated fullerenes to interfere with the evolutionary conserved innate immune system responses and encourages the use of fish models in studies of nanoparticle immunotoxicity.

ACS Style

Boris Jovanović; Lora Anastasova; Eric W. Rowe; Dušan Palić. Hydroxylated fullerenes inhibit neutrophil function in fathead minnow (Pimephales promelas Rafinesque, 1820). Aquatic Toxicology 2011, 101, 474 -482.

AMA Style

Boris Jovanović, Lora Anastasova, Eric W. Rowe, Dušan Palić. Hydroxylated fullerenes inhibit neutrophil function in fathead minnow (Pimephales promelas Rafinesque, 1820). Aquatic Toxicology. 2011; 101 (2):474-482.

Chicago/Turabian Style

Boris Jovanović; Lora Anastasova; Eric W. Rowe; Dušan Palić. 2011. "Hydroxylated fullerenes inhibit neutrophil function in fathead minnow (Pimephales promelas Rafinesque, 1820)." Aquatic Toxicology 101, no. 2: 474-482.