Fresh peaches and nectarines are very popular for their high nutritional and therapeutic value. Unfortunately, they are prone to rapid deterioration after harvest, especially if the cold chain is not well maintained. The objective of this work is to study the environmental fluctuation and the quality change of fresh peaches and nectarines in cold chain. The temperature, relative humidity, and CO₂ level were real-time monitored by sensor nodes with a wireless sensor network (WSN). The cold chain lasted for 16.8 h and consisted of six segments. The dynamic change of temperature, relative humidity, and CO₂ level were real-time monitored and analyzed in detail in each of the six stages. The fruit quality index (fruit weight, fruit firmness, and soluble solids concentration (SSC)) were detected and analyzed immediately before the first stage (S1) and at the beginning of the last stage (S6). The results show that without good temperature control fruit softening is the most significant problem, even in a short chain; the WSN node can provide complete and accurate temperature, humidity, and gas monitoring information for cold chains, and can be used to further improve quality and safety assurance for peach fruit cold chains.

In order to fully understand the variation of the fruit alpha-glucosidase inhibitory activity-related phytochemical basis in the Chinese peach [Prunus persica (L.) Batsch], mature fruit from 33 cultivars was used for the investigation of fruit phenolic phytochemical attributes, including total phenolics, flavonoids, anthocyanins, and procyanidins, as well as the alpha-glucosidase inhibitory activity in vitro. Alpha-glucosidase inhibitory activity varied significantly among tested peach cultivars and was strongly correlated with total phenolics, total procyanidins, and total flavonoids. Untargeted UPLC-Q-TOF/MS-based metabolomics were used to comprehensively discriminate between peaches with different inhibitory activity on alpha-glucosidase. Principal component analysis (PCA) and orthogonal partial least squares discrimination analysis (OPLS-DA) were used for this process. Twenty-three differential compounds were identified between peach cultivars with high and low alpha-glucosidase inhibitory activity, and nine, including procyanidin C1, procyanidin trimer isomer 1, procyanidin trimer isomer 2, procyanidin B1, procyanidin dimer, epicatechin-epicatechin-epicatechin, phloridzin, kaempferol 3-(2’’,6’’-di-(E)-p-coumarylglucoside), and luteolin 3’-methyl ether 7-malonylglucoside, were identified as marker compounds responsible for the discrimination. Overall, variations in metabolites in peach pulp reflect the diversity in peach germplasm, and these nine compounds are good candidate markers for future genetic breeding of peach fruit with high alpha-glucosidase inhibitory activity.

Cold storage is used to delay the senescence of peaches, but it can also lead to internal browning and aroma loss. Modified atmosphere packaging (MAP) has been reported to inhibit the internal browning and prolong the storage time. Four MAP treatments in the present study were set as follows: I: O ₂ 1% to 3%, CO ₂ 3% to 5%, and N ₂ 92% to 96%; II: O ₂ 3% to 5%, CO ₂ 3% to 5%, and N ₂ 90% to 94%; III: O ₂ 6% to 8%, CO ₂ 3% to 5%, and N ₂ 87% to 91%; and control (CK): O ₂ 21%, CO ₂ 0.03%, and N ₂ 79%. The concentration of sugars, acids, aroma compounds, superoxide radical (O ₂ ⁻ ), hydrogen peroxide (H ₂ O ₂ ), and malondialdehyde (MDA), as well as the activities of enzymes, such as superoxide dismutase (SOD), peroxidase (POD), lipoxygenase (LOX), hydroperoxide lyase (HPL), alcohol dehydrogenase, and alcohol O -acyltransferase (AAT) activities, were investigated. The results revealed that MAP, especially for treatment II, could inhibit the loss of flavors such as sugars, acids, and aroma compounds; maintain higher SOD and POD activities; and inhibit the accumulation of O ₂ ⁻ , H ₂ O ₂ , and MDA during shelf life after storage at low temperature for 30 days. It could also inhibit the LOX and HPL activities at low temperature, but maintain higher LOX and HPL activities during shelf life. These findings indicated that treatment II could prolong the storage time to 30 days and shelf life for 3 days; maintain the higher content of sugars, acids, and aroma compounds; protect the cell membrane from oxidative injury; and inhibit internal browning during cold storage and shelf life.