Investigations have indicated that storage pests pose a great threat to global food security by damaging food crops and other food products derived from plants. Essential oils are proven to have significant effects on a large number of stored grain insects. This study evaluated the contact toxicity and fumigant activity of the essential oil extract from the aerial parts of Elsholtzia ciliata and its two major biochemical components against adults and larvae of the food storage pest beetle Tribolium castaneum. Gas chromatography–mass spectrometry analysis revealed 16 different components derived from the essential oil of E. ciliata, which included carvone (31.63%), limonene (22.05%), and α-caryophyllene (15.47%). Contact toxicity assay showed that the essential oil extract exhibited a microgram-level of killing activity against T. castaneum adults (lethal dose 50 (LD₅₀) = 7.79 μg/adult) and larvae (LD₅₀ = 24.87 μg/larva). Fumigant toxicity assay showed LD₅₀ of 11.61 mg/L air for adults and 8.73 mg/L air for larvae. Carvone and limonene also exhibited various levels of bioactivity. A binary mixture (2:6) of carvone and limonene displayed obvious contact toxicity against T. castaneum adults (LD₅₀ = 10.84 μg/adult) and larvae (LD₅₀ = 30.62 μg/larva). Furthermore, carvone and limonene exhibited synergistic fumigant activity against T. castaneum larvae at a 1:7 ratio. Altogether, our results suggest that E. ciliata essential oil and its two monomers have a potential application value to eliminate T. castaneum.

Storage pests pose a great threat to global food security. Here, we found that the essential oil (EO) extracted from E. densa possesses obvious effects against the insects that threaten stored-products. In this work, we investigated the chemical constituents of the essential oil extracted from Elsholtzia densa, and their insecticidal (contact and fumigant) toxicity against Tribolium castaneum and Lasioderma serricorne. A total of 45 compounds were identified by GC-MS, accounting for 98.74% of the total EO. Meanwhile, 11 compounds were isolated from the EO, including limonene, β-caryophyllene, ρ-cymene, trans-phytol, α-terpineol, linalool, acetophenone, 1,8-cineole, ρ-cymen-7-ol, 1-O-cerotoylgly-cerol, and palmitic acid. Furthermore, acetophenone, ρ-cymen-7-ol, and 1-O-cerotoylgly-cerol were isolated for the first time from Elsholtzia spp. The results of the bioassays indicated that the EO had the property of insecticidal toxicity against T. castaneum and L. serricorne. All of the compounds showed different levels of insecticidal toxicity against the two species of insects. Among them, 2-ethyl-1H-imidazole had no insecticidal toxicity against T. castaneum, but possessed fumigant and obvious contact toxicity against L. serricorne. ρ-Cymen-7-ol had beneficial insecticidal toxicity against the two species of insects, and fumigant toxicity against L. serricorne. ρ-Cymen-7-ol (LD₅₀ = 13.30 μg/adult), 1-octen-3-ol (LD₅₀ = 13.52 μg/adult), and 3-octanol (LD₅₀ = 17.45 μg/adult) showed significant contact toxicity against T. castaneum. Acetophenone (LD₅₀ = 7.07 μg/adult) and ρ-cymen-7-ol (LD₅₀ = 8.42 μg/adult) showed strong contact toxicity against L. serricorne. ρ-Cymene (LC₅₀ = 10.91 mg/L air) and ρ-cymen-7-ol (LC₅₀ = 10.47 mg/L air) showed powerful fumigant toxicity to T. castaneum. Limonene (LC₅₀ = 5.56 mg/L air), acetophenone (LC₅₀ = 5.47 mg/L air), and 3-octanol (LC₅₀ = 5.05 mg/L air) showed obvious fumigant toxicity against L. serricorne. In addition, the EO and its chemical compounds possessed different levels of repellent activity. This work provides some evidence of the value of exploring these materials for insecticidal activity, for human health purposes. We suggest that the EO extracted from E. densa may have the potential to be developed as an insecticidal agent against stored product insect pests.

This work investigated the chemical constituents of the essential oil (EO) extracted from different organs (whole plants, flower and fruits, leave and branches) of Caryopteris mongholica. At the same time, the fumigant, contact and repellent effect of the EO from C. mongholica against Lasioderma serricorne and Tribolium castaneum were evaluated. The main chemical components were identified from the EO included β-pinene (24.77 %), 4-terpineol (15.80 %), β-cis-ocimene (9.31 %), α-pinene (8.20 %), 2-isopropyltoluene (7.37 %). The results of bioassays indicated that EO was toxicity to T. castaneum than L. serricorne. The results also showed that possessed stronger repellent activity against T. castaneum than L. serricorne. The results provided a basis for the development and utilization in the future for the control of insects.