The continued dispersal of Fusarium oxysporum f. sp. cubense Tropical race 4 (FocTR4), a quarantine soil-borne pathogen that kills banana, has placed this worldwide industry on alert and triggered enormous pressure on National Plant Protection (NPOs) agencies to limit new incursions. Accordingly, biosecurity plays an important role while long-term control strategies are developed. Aiming to strengthen the contingency response plan of Ecuador against FocTR4, a population biology study—including phylogenetics, mating type, vegetative compatibility group (VCG), and pathogenicity testing—was performed on isolates affecting local bananas, presumably associated with race 1 of F. oxysporum f. sp. cubense (Foc). Our results revealed that Foc populations in Ecuador comprise a single clonal lineage, associated with VCG0120. The lack of diversity observed in Foc populations is consistent with a single introduction event from which secondary outbreaks originated. The predominance of VCG0120, together with previous reports of its presence in Latin America countries, suggests this group as the main cause of the devastating Fusarium wilt epidemics that occurred in the 1950s associated to the demise of ‘Gros Michel’ bananas in the region. The isolates sampled from Ecuador caused disease in cultivars that are susceptible to races 1 and 2 under greenhouse experiments, although Fusarium wilt symptoms in the field were only found in ‘Gros Michel’. Isolates belonging to the same VCG0120 have historically caused disease on Cavendish cultivars in the subtropics. Overall, this study shows how Foc can be easily dispersed to other areas if restriction of contaminated materials is not well enforced. We highlight the need of major efforts on awareness and monitoring campaigns to analyze suspected cases and to contain potential first introduction events of FocTR4 in Ecuador.

Cacao demand is continuously increasing, and variations in cacao prices have been associated with the aroma of fermented cacao beans. However, the role of microorganisms in the formation of volatile-aroma compounds during fermentation remains unclear. Microbial diversity in Nacional × Trinitario cacao was characterized during spontaneous fermentation by using culture-based methods and next-generation sequencing (NGS) of DNA amplicons. Cacao beans that were spontaneously fermented for 0, 24, 48, 72 and 96 h were UV-sterilized prior to the inoculation of the microbial isolates obtained by the culture-based methods. The volatile formation in inoculated cacao beans was evaluated by GC-MS. The species isolated during fermentation included yeast, such as Saccharomyces cerevisiae and Candida metapsilosis; lactic acid bacteria (LAB), such as Limosilactobacillus fermentum and Liquorilactobacillus nagelii; acetic acid bacteria (AAB), such as Acetobacter pasteurianus, Acetobacter ghanensis and Acetobacter syzygii, as well as other species, such as Bacillus subtilis and Bacillus amyloliquefaciens. Additionally, NGS revealed an abundance of environmental microorganisms, including Escherichia spp., Pantoea spp., Staphylococcus spp., Botrytis spp., Tetrapisispora spp. and Pichia spp., among others. During the lab-scale fermentation, the inoculation of S. cerevisiae mostly yielded alcohols, while LAB and AAB produced volatiles associated with floral, almond and fruity notes throughout the fermentation, but AAB also produced acetic acid with a sour aroma. Similarly, the inoculation of C. metapsilosis and Bacillus spp. in 96 h fermented cacao beans yielded esters with floral aromas. This is the first report describing the role of microorganisms in volatile formation during fine-flavor cacao fermentation.

Environmental pollution caused by mercury has received increasing attention in recent years. Several studies have warned of the high rates of biomagnification in superior levels of marine food networks affecting seabirds. Although seabird feathers are reported as bioindicators of mercury, the possibility of using the microbiota associated with them for the bioremediation of this metal has not been considered. Despite the potential of the seabird feather microbiota, the cultivable microorganisms from this sample matrix have not been identified. In this study, we isolated and identified the organisms in the feathers from three types of seabirds, two species of penguins (Pygoscelis antartica and Pygoscelis papua) and the brown skua bird (Catharacta lonnbergi) through poisoned media a final concentration of 10 mg / L Hg2+ in the culture medium for the microbial consortia. Yeast isolates belonged to the genus Debaryomyces, Meyerozyma, Papiliotrema, and Rhodotorula, and fungi genera Leiotrametes, Penicillium, Pseudogymnoascus, and Cladosporium were identified. Adult bird feathers with high mercury concentrations can serve as a matrix to isolate microorganisms capable of removing mercury. Keywords: Antarctica, bioremediation, feathers, mercury, microorganisms

Bananas (Musa spp.), often reported together with plantains, are among the most traded fruit commodities worldwide, particularly in terms of export volume and value, with Ecuador recognized as the leading global exporter, supplying approximately one quarter of the total international trade. However, its production is increasingly threatened by climate change and fungal diseases such as Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4). To support preventive management strategies against Foc TR4, this study evaluated the in vitro efficacy of nine commercial disinfectants available in Ecuador—including quaternary ammonium compounds (QACs), oxidizing agents, aldehydes, and alkaline substances—against conidia and chlamydospores of several Foc race 1 strains (VCG0120) as a model organism. Different concentrations and contact times were tested in the presence and absence of organic matter. Chlamydospores exhibited higher resistance than conidia, requiring greater concentrations for inhibition. Aldehyde formulations and QACs achieved the lowest minimum inhibitory concentrations (<1,000 ppm) and remained effective under organic load and variable contact times. These findings emphasize the need to select disinfectants with consistent efficacy under field-relevant conditions to ensure the application of proper biosecurity measures.