Background and Objectives CSF in antibody-defined autoimmune encephalitis (AE) subtypes shows subtype-dependent degrees of inflammation ranging from rare and often mild to frequent and often robust. AEs with NMDA receptor antibodies (NMDAR-E) and leucine-rich glioma-inactivated protein 1 antibodies (LGI1-E) represent opposite ends of this spectrum: NMDAR-E with typically frequent/robust and LGI1-E with rare/mild CSF inflammation. For a more in-depth analysis, we characterized CSF findings in acute, therapy-naive NMDAR-E and LGI1-E in a multicentric, retrospective, cross-sectional setting. Methods Eighty-two patients with NMDAR-E and 36 patients with LGI1-E from the GErman NEtwork for Research of AuToimmune Encephalitis (GENERATE) with lumbar puncture within 90 days of onset and before immunotherapy were included. CSF parameters comprised leukocytes, oligoclonal bands (OCBs), and CSF/serum ratios for albumin, immunoglobulin G (IgG), A (IgA), and M (IgM), the latter 3 converted to Z scores according to Reiber formulas. The MRZ reaction was tested in 14 patients with NMDAR-E and 6 patients with LGI1-E, respectively. Results CSF was abnormal in 94% of NMDAR-E but only in 36% of LGI1-E patients. Robust quantitative intrathecal immunoglobulin synthesis (IIS, IgG > IgM >> IgA) was characteristic for NMDAR-E, but absent in LGI-E. In NMDAR-E, CSF leukocytes were higher when IIS was present or more pronounced. In addition, in NMDAR-E, CSF leukocytes were lower and IIS occurred less often and if so to a lesser degree at older age. Patients with NMDAR-E with severe functional impairment more often had positive OCBs. In CSF obtained later than 3 weeks of onset, leukocytes were lower. In parallel, the correlation of leukocytes with IIS disappeared as IIS was partially independent of disease duration. The MRZ reaction was positive in 5 (36%) patients with NMDAR-E. All these associations were completely absent in LGI1-E. Here, younger patients showed more blood-CSF barrier dysfunction. In LGI1-E, but not in NMDAR-E, the blood-CSF barrier was more dysfunctional when CSF leukocytes were higher. Discussion NMDAR-E and LGI-E differ in their typical extent of CSF inflammation. In addition, the patterns formed by the different inflammatory CSF parameters and their relationship with disease severity, age, and disease duration are subtype-characteristic. Moreover, signs for multiple sclerosis-like chronic inflammation are present in a subgroup of patients with NMDAR-E. These CSF patterns might be markers for the different immunopathogeneses of LGI1-E and NMDAR-E.

Increasing prevalences of overweight and obesity in children are known problems in industrialized countries. Early prevention is important as overweight and obesity persist over time and are related with health problems later in adulthood. "Komm mit in das gesunde Boot - Grundschule" is a school-based program to promote a healthier lifestyle. Main goals of the intervention are to increase physical activity, decrease the consumption of sugar-sweetened beverages, and to decrease time spent sedentary by promoting active choices for healthy lifestyle. The program to date is distributed by 34 project delivery consultants in the state of Baden-Württemberg and is currently implemented in 427 primary schools. The efficacy of this large scale intervention is examined via the Baden-Württemberg Study.

Only a small proportion of children and adolescents meet current recommendations of at least 60 min of moderate to vigorous physical activity (MVPA) daily. Most of the available data, however, relies on subjective reports; there is limited objective data on physical activity (PA) levels in German primary school children. The purpose of this study, therefore, was to accurately determine how much time children spend undertaking different intensities of PA and being sedentary during weekdays and weekend using objective assessment tools. Gender-specific and age-related differences were examined along with differences between normal weight and overweight/obese children.

Sedentary behaviour has become a growing public health concern. Currently, it is a common belief that screen time (SCT) is a key factor in high overall sedentary time (ST) and is often used as a primary outcome. However, the evidence is lacking. Therefore, this study investigated the association of objectively assessed total ST with SCT among children. Further, SCT was investigated separately for sedentary level, weight status, gender, and migration background. For 198 primary school children (7.1 ± 0.7 years, boys: 43.9%) ST was assessed objectively using a multi-sensor device (Actiheart®; CamNtech, Cambridge, UK). The sample was split into three groups (tertiles) to investigate SCT of children with low, medium and high ST. SCT and socio-demographic parameters, such as migration background, were assessed using a parental questionnaire; anthropometric data was collected at schools. Absolut SCT did not differ significantly among the three sedentary groups: Daily average of SCT was 83.8 ± 55.0 min (27.4% of ST) for children with high ST, 82.8 ± 50.5 min (39.8% of ST) for children with medium ST, and 77.2 ± 59.4 min (71.3% of ST) for those with low ST. However, relatively the SCT percentage of total ST was significantly higher among children with low ST (p < 0.01). Significantly higher SCT was found in children with migration background (p < 0.01), while underweight children had significantly less SCT (p < 0.05). An association of total SCT and overall ST was found for the whole sample (B = 17.11, [2.75; 31.48], p = 0.02), but did not remain when analysis were separated for the groups, except for normal weight children (B = 15.97, [0.13; 31.81], p = 0.05). The amount of SCT is the same among high, low and medium sedentary children, and high ST is largely independent of SCT. Therefore, SCT cannot be the key contributor to high ST and should not solely be used for predicting or changing children’s sedentary behaviour. Moreover, children’s weight status to classify activity levels and the role of possible compensation mechanisms should be considered in future research and when trying to intervene on ST. German Clinical Trials Register (DRKS), DRKS-ID: DRKS00000494 DATE: 25/08/2010.

Childhood obesity and its consequences are a growing threat to national economies and health services. The aim of this study was to determine associations between waist-to-height ratio (WHtR) as a measure of central obesity, and health-related quality of life (HRQoL) and absenteeism of primary school children in the state of Baden-Württemberg, Germany.