Soil-borne pathogenic fungi (SBPF) and root-knot nematodes (RKN) co-exist in the rhizosphere and are major pathogens causing root diseases in cucurbits. Current knowledge on soil-borne pathogens of cucurbit crops grown under protected cultivation, their host-pathogen interactions, and mechanisms of resistance has been reviewed. Plant resistance is an effective and sustainable method to control soil-borne diseases and the available resistant cultivars and rootstocks to key soil-borne pathogens are reported. The importance of proper pathogen diagnosis in the right choice of cultivar or rootstock is highlighted because of the specificity in the response of the cucurbit crops to fungal and nematode species and races. Plants protect themselves through common mechanisms of resistance against SBPF and RKN including hardening of their cell walls, pathogenesis-related (PR) proteins, and production of antimicrobial molecules. The activity of some enzymes, such as peroxidases and phenylalanine lyase, is increased after pathogen infection and is higher on SBPF and RKN resistant than susceptible cucurbits. Plant hormones such as salicylic acid, jasmonic acid, and ethylene are involved in the response of cucurbits to SBPF. Most mechanisms of resistance to RKN affect post-infection development of the nematode, which results in a delay or disruption of the life cycle. Traditional and biotechnological tools used for breeding for resistance in cucurbits are described. Grafting is an effective non-host resistance method to control primarily Fusarium wilt but not to control RKN. However, new rootstocks with resistance to both pathogens have been developed recently and their effects on fruit quality and yield stability need additional studies. The impact of grafting on yield in pathogen-infested soils is discussed.

Phytophtora capsici causes major diseases in cucurbit crops worldwide. In this study, we inoculated this pathogen into Cucurbita pepo subsp. pepo susceptible MUCU-16 and C. moschata tolerant M63. The gene expression of plant pathogenesis-related proteins chitinase (CpChiIV), lignin-forming peroxidase (CpLPOX), and defensin (CpDEF) and hormone-related enzymes salicylic acid (CpPAL) and ethylene (CpACO) was analyzed for two weeks post-inoculation in root and crown tissues. Differentially expressed genes were found between genotypes, tissues, days post-inoculation, and inoculated/non-inoculated samples. After inoculation, CpPAL and CpChiIV (crown) were downregulated in MUCU-16, while CpLPOX and CpDEF were upregulated in M63. In inoculated samples, higher expression changes were presented on days 10–14 than on day 3 for CpACO, CpLPOX, and CpDEF genes. Overexpression was higher for CpDEF compared to the other tested genes, indicating good suitability as a marker of biotic stress. The overexpression of CpDEF was higher in crown than in roots for both inoculated genotypes. The basal expression of CpPAL and CpDEF was higher in MUCU-16, but after inoculation, CpPAL and CpDEF gene expression were higher in M63. These changes suggest an association between CpDEF upregulation and tolerance, and between CpPAL downregulation and susceptibility.

Cucurbita species can be affected by soil-borne pathogens, such as Phytopthora capsici and Fusarium solani f. sp. cucurbitae (Fsc). Diverse commercial and conserved lines of Cucurbita spp. were tested. C. pepo subsp. pepo genotypes showed the highest susceptibility to both pathogens. The tolerance to P. capsici and Fsc was then screened in a zucchini mutant population. Two M3 mutant lines (Cp107 and Cp116) with a high occurrence of tolerant individuals to Phytophthora capsici were obtained from a screening of 160 M2 mutant lines. The M3 lines presented higher tolerance than the background MUCU-16. Furthermore, in the inoculated samples, both mutants overexpressed CpDEF and expressed more CpPAL and CpChiIV than the susceptible control. It has been previously shown that this expression pattern could be associated with tolerance in the P. capsici - Cucurbita spp. pathosystem. The M3 lines obtained could be applied in breeding programs, as they are likely to be compatible with the highly susceptible C. pepo subsp. pepo genotype.

The study of fruit development in zucchini via gene expression has proven to be applicable in breeding programs. Phenotypic and transcriptomic studies of fruit set and parthenocarpy have been previously developed and some relevant genes have been reported. From these studies, three genotypes (MUCU-16, Whitaker, and Cavili) and six genes (CpAUX22, CpIAA4, CpIAMT-1, CpPIN5, CpCYCD6-1, and CpEXPLB1) were selected. The expression of these genes was analyzed in each genotype under three different treatments (pollination, auxin-treatment and non-treatment) during one week post anthesis. Also, a phenotyping analysis was conducted. The different nature of the samples and the genes selected allowed associations between different fruit traits and fruit development stages. There was a rapid response of CpAUX22 and CpIAA4 to the auxin treatment. Also, these genes and the CpIAMT-1 became more overexpressed in pollinated samples over time. The CpPIN5 gene increased its expression over time in all genotypes while CpCYCD6-1 was overexpressed in the early stages of fruit development in all samples. The CpEXPLB1 was highly up-regulated in non-treated samples, suggesting a relationship with fruit abortion. The overexpression of CpAUX22 and the non-overexpression of CpEXPLB1 in early stages may be associated with fruit growth in zucchini.