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Watermelon is the most important horticultural crop in Texas and is grown across the state under diverse environments. Our study was conducted in the southern region of Texas to understand genotype-by-environment interactions and the contribution of yield components to yield. To accomplish this, twenty genotypes were evaluated for important traits and characteristics at two locations, Uvalde and Weslaco TX, for two years, 2018 and 2019. The genotypes were evaluated for total yield, total fruit count, total soluble solids, rind thickness, fruit length, diameter and weight. Genotype-by-environment (G x E) interaction was not significant, possibly due to similarity in climatic conditions and nutrient management practices. In the grouped analysis, cultivars Crimson Diamond, Sunshade and the breeding line TAM 2 had a higher total yield. Path analysis showed a high direct effect for total fruit count and fruit diameter of 0.89 and 0.85, respectively. However, total fruit count had a high indirect effect of −0.44. Fruit weight was the only trait that showed a significant (p < 0.01) correlation towards total yield at r = 0.58. Neither of the high direct effects, total fruit count and fruit diameter, had a significant correlation. The study inferred that breeding resources could be optimized by reducing the testing location to only one representative location for measured traits in southern Texas. The indirect selection of total fruit or fruit diameter could result in better yield. The study suggested selecting for optimum total fruit and fruit diameter for higher yield.
Edgar Correa; Subas Malla; Kevin Crosby; Carlos Avila. Evaluation of Genotypes and Association of Traits in Watermelon Across Two Southern Texas Locations. Horticulturae 2020, 6, 67 .
AMA StyleEdgar Correa, Subas Malla, Kevin Crosby, Carlos Avila. Evaluation of Genotypes and Association of Traits in Watermelon Across Two Southern Texas Locations. Horticulturae. 2020; 6 (4):67.
Chicago/Turabian StyleEdgar Correa; Subas Malla; Kevin Crosby; Carlos Avila. 2020. "Evaluation of Genotypes and Association of Traits in Watermelon Across Two Southern Texas Locations." Horticulturae 6, no. 4: 67.
Horticultural crops include a diverse array of crops comprising fruits, vegetables, nuts, flowers, aromatic and medicinal plants. They provide nutritional, medicinal, and aesthetic benefits to mankind. However, these crops undergo many biotic (e.g., diseases, pests) and abiotic stresses (e.g., drought, salinity). Conventional breeding strategies to improve traits in crops involve the use of a series of backcrossing and selection for introgression of a beneficial trait into elite germplasm, which is time and resource consuming. Recent new plant breeding tools such as clustered regularly interspaced short palindromic repeats (CRISPR) /CRISPR-associated protein-9 (Cas9) technique have the potential to be rapid, cost-effective, and precise tools for crop improvement. In this review article, we explore the CRISPR/Cas9 technology, its history, classification, general applications, specific uses in horticultural crops, challenges, existing resources, associated regulatory aspects, and the way forward.
Bed Prakash Bhatta; Subas Malla. Improving Horticultural Crops via CRISPR/Cas9: Current Successes and Prospects. Plants 2020, 9, 1360 .
AMA StyleBed Prakash Bhatta, Subas Malla. Improving Horticultural Crops via CRISPR/Cas9: Current Successes and Prospects. Plants. 2020; 9 (10):1360.
Chicago/Turabian StyleBed Prakash Bhatta; Subas Malla. 2020. "Improving Horticultural Crops via CRISPR/Cas9: Current Successes and Prospects." Plants 9, no. 10: 1360.
Use of genetic resistance is one of the most important strategies to manage the devastating disease Fusarium Head Blight (FHB) in wheat. Numerous QTL having varying effects on reducing FHB and mycotoxin deoxynivalenol (DON) accumulation have been reported from Asian, European or distant sources such as wild relatives of wheat. However, coming from non‐adapted backgrounds, the incorporation of such QTL into regional breeding programs has often resulted in the simultaneous transfer of other undesirable traits. Therefore, it is important to identify, characterize and deploy sources of genetic resistance that do not suffer from poor adaptability and/or linkage drag. In the present work, QTL associated with FHB resistance in a high yielding, moderately resistant soft red winter wheat variety Jamestown were mapped and validated. QTL mapping was done using a Recombinant Inbred Line (RIL) population of Pioneer‘25R47’ × ‘Jamestown’ having 186 individuals. Phenotyping over two years at three locations, and genotyping using the 90K‐SNP platform identified two new QTL, named QFHB.vt‐1B.1 and QFHB.vt‐1B.2, on chromosome 1B long arm. The QTL contributed to FHB incidence, FHB severity, Fusarium Damaged Kernels, and DON content. Independent mapping of these QTL using two additional RIL populations of FG95195 × Jamestown (170 RILs) and Jamestown × LA97113UC‐124 (77 RILs) validated their stability and effectiveness in different genetic backgrounds. KASP assays were developed using linked SNPs for Marker assisted selection of the QTL. These QTL are being used in breeding programs to develop FHB resistant high yielding varieties. This article is protected by copyright. All rights reserved
Neal R. Carpenter; Emily Wright; Subas Malla; Lovepreet Singh; David Van Sanford; Anthony Clark; Stephen Harrison; J. Paul Murphy; Jose Costa; Shiaoman Chao; Gina L. Brown‐Guedira; Nicole McMaster; David G. Schmale; Carl A. Griffey; Nidhi Rawat. Identification and validation of Fusarium head blight resistance QTL in the U.S. soft red winter wheat cultivar ‘Jamestown’. Crop Science 2020, 60, 2919 -2930.
AMA StyleNeal R. Carpenter, Emily Wright, Subas Malla, Lovepreet Singh, David Van Sanford, Anthony Clark, Stephen Harrison, J. Paul Murphy, Jose Costa, Shiaoman Chao, Gina L. Brown‐Guedira, Nicole McMaster, David G. Schmale, Carl A. Griffey, Nidhi Rawat. Identification and validation of Fusarium head blight resistance QTL in the U.S. soft red winter wheat cultivar ‘Jamestown’. Crop Science. 2020; 60 (6):2919-2930.
Chicago/Turabian StyleNeal R. Carpenter; Emily Wright; Subas Malla; Lovepreet Singh; David Van Sanford; Anthony Clark; Stephen Harrison; J. Paul Murphy; Jose Costa; Shiaoman Chao; Gina L. Brown‐Guedira; Nicole McMaster; David G. Schmale; Carl A. Griffey; Nidhi Rawat. 2020. "Identification and validation of Fusarium head blight resistance QTL in the U.S. soft red winter wheat cultivar ‘Jamestown’." Crop Science 60, no. 6: 2919-2930.