K (K) is of paramount importance for apple (Malus × domestica Borkh.), not only for tree growth and development but also for the size and quality of fruit yield. The apple plant’s demand for K varies, along with the progression of phenological phases, during the growing season. The K demand peaks during ripening of fruits featuring relatively high concentration of K comparable to that of the leaves. The mainstream method of apple tree K fertilization is through application of the fertilizer to the soils to improve K uptake by the roots. The bioavailability of K depends on assorted various factors, including pH, interaction with other nutrients in soil solution, temperature, and humidity. An important role in making the K from soil available for uptake by plants is played by plant growth-promoting microorganisms (PGPM), and the specific role of the PGPM is discussed. Advantages of fertigation (the combination of irrigation and fertilization) as an approach include allowing to balance application rate of K fertilizer against its variable demand by plants during the growing season. Excess K in the soil leads to competitive inhibition of calcium uptake by plants. The K-dependent deficiency of Ca leads to its predominant channeling to the leaves and hence to its decline in fruits. Consequently, the apple fruits affected by the K/Ca imbalance frequently develop physiological disorders in storage. This emphasizes the importance of the balanced K application, especially during the last months of the growing season, depending on the crop load and the actual K demand. The potential use of modern approaches to automated crop load estimation through machine vision for adjustment of K fertilization is underlined.
The proper use of potassium fertilizer can stimulate a significant yield increase. However, the application of excessively high rates of potassium can reduce the availability of soil calcium for apple trees. The potassium fertigation rate must meet the apple tree’s requirements so that the applied fertilizers can be absorbed by the roots as much as possible. Crop load in apple orchards sometimes varies significantly in different years. The potassium content in apple fruits is relatively high, and the maximum requirement for this nutrient occurs when fruits grow and ripen. Different crop loads at that time mean the various demands of trees and need for changing application rates for this nutrient. The investigation was carried out in the experimental orchard of I.V. Michurin Federal Scientific Centre (Michurinsk, Russia) in 2016 and 2017 (52.885131, 40.465613). We studied seasonal changes of potassium and calcium contents in soil, fruits, and leaves and their relationship with yield during the research. We paid much attention to the potassium rate shift on its content in leaves and fruits and cultivars “Lobo” and “Zhigulevskoye” yield. If the potassium application rate changes according to the actual crop load, it stimulates the yield growth or (if the crop load was relatively low) the reduction of the rate did not lower the productivity. Moreover, we studied the relationship between potassium and calcium nutrition. The decrease in potassium fertigation rate increased the availability of soil calcium. It was the reason for fruit calcium concentration enlargement and mitigation of the K/Ca ratio. We also specified some parameters for soil–leaf diagnosis for potassium nutrition during the growing season.
Reflected light carries ample information about the biochemical composition, tissue architecture, and physiological condition of plants. Recent technical progress has paved the way for affordable imaging hyperspectrometers (IH) providing spatially resolved spectral information on plants on different levels, from individual plant organs to communities. The extraction of sensible information from hyperspectral images is difficult due to inherent complexity of plant tissue and canopy optics, especially when recorded under ambient sunlight. We report on the changes in hyperspectral reflectance accompanying the accumulation of anthocyanins in healthy apple (cultivars Ligol, Gala, Golden Delicious) fruits as well as in fruits affected by pigment breakdown during sunscald development and phytopathogen attacks. The measurements made outdoors with a snapshot IH were compared with traditional “point-type” reflectance measured with a spectrophotometer under controlled illumination conditions. The spectra captured by the IH were suitable for processing using the approaches previously developed for “point-type” apple fruit and leaf reflectance spectra. The validity of this approach was tested by constructing a novel index mBRI (modified browning reflectance index) for detection of tissue damages on the background of the anthocyanin absorption. The index was suggested in the form of mBRI = (R640−1 + R800−1) − R678−1. Difficulties of the interpretation of fruit hyperspectral reflectance images recorded in situ are discussed with possible implications for plant physiology and precision horticulture practices.
The application of plasma-activated water (PAW) in agriculture has gained the attention of researchers and practitioners. In particular, treatment with PAW is a promising method for increasing scion and rootstock survival as well as augmenting the mineral nutrition applicable to tree fruit crops. However, the applications of PAW are hampered by the lack of information about the effects of PAW on apple tree condition and yield. The increase in survival rate by PAW is believed to stem from the general stimulation of physiological processes in the plant tissue. To assess the actual effect of the PAW treatments, one needs to consider an important indicator of young tree quality such as their vegetative growth. We conducted field experiments to study the possibility of use of PAW for increase in primary nutrient contents in fruits and leaves in an orchard, as well as to assess the scion survival rate and vegetative growth of young grafts in a nursery. The application of PAW influenced the fruitset, yield, leaf nitrogen (N) and potassium (K), fruit phosphorus (P), calcium (Ca) ascorbic acid (AA) and titratable acidity (TA). Treatment with PAW did not significantly reduce the negative impact of the rootstock thickness on the survival rate of bench grafts and their subsequent development. At the same time, scion survival tended to increase in the case when the scions and the rootstocks were of compatible thickness. Further studies of the PAW treatment effects are needed to better understand its applicability in diverse fields of horticulture.
Intensification of horticulture in Russia involves planting of new high-density orchards with drip irrigation and fertigation as well as intensification of the exploitation of traditional orchards. This approach involves an increase in mineral fertilizer application imposing the risk of soil fertility loss. For several reasons, the use of traditional organic fertilizers like manure in orchards is currently marginal. Although bacteria-based biofertilizers cannot substitute mineral fertilizers completely, they can significantly reduce the need for mineral fertilizer application. The effect of microbial biofertilizers of the brands “Azotovit” (Azotobacter chroococcum), “Phosphatovit” (Bacillus mucilaginosus), as well as a mixture of bacteria and the fungus, “Organic” (Azotobacter chroococcum, Bacillus subtilis, Bacillus megaterium, Trichoderma harzian) was studied in two field experiments. In the experiment #1, the preparations “Azotovit” and “Phosphatovit” were delivered through a drip irrigation system in various combinations with mineral fertilizers. In experiment #2, the preparation “Organic” was also applied to the soil with irrigation water, also in combination with the mineral fertilizer. When solely applied, none of the studied preparations changed significantly the soil nutrient content and yield as compared with the variant fertilized by the mineral fertilizer at the maximum studied application rate. The combination of the microbial biofertilizer and mineral fertilizers applied at a low rate ensured the yield commensurate to that obtained under high-rate application of the mineral fertilizer.
Foliar fertilizing is very important to supply apple plants with calcium and micronutrients. The most cost-effective approach to this is the application of the fertilizers in tank mixtures with plant protection chemicals. Plasma-activated water (PAW) has great potential for the use in the agriculture. We used two type of PAWs, PAW1 (made using underwater electrical discharge in an aqueous KNO3 solution and includes reactive nitrogen species and platinum nanoparticles) and PAW2 (made using a plasma torch with nitrogen gas makeup and contains reactive nitrogen species but not metals). We studied the impact of two PAW types on the contents of Ca, B, Mn, Fe, and Co in leaves and Ca, Mn, Fe, Zn, and Mo in fruits sprayed with tank mixtures containing the fertilizers. We also tested the efficiency of PAW in the control of apple scab when applied as tank mixtures with plant protection chemicals. The application of the PAWs significantly increased foliar Ca when the PAW was mixed with Ca-containing formulations (spraying PAW1 containing Ca increased leaf Ca by up to 21%, and PAW2 up by to 9% compared to Ca spraying without PAW). The largest fruit Ca increase was in the variant treated with PAW1 with a micronutrient spraying program (up to 143%). The PAW treatments enhanced the baseline mineral contents of the plants even when they were not sprayed with the nutrients. PAW1 mainly increased the nutrient contents of the apple fruits. PAWs have proven to be efficient for the control of apple scab, thereby reducing the demand for fungicides. The scab damage to the leaves and fruits was similar in plants treated with PAWs without fungicides (1.7–1.9% on the leaves and 1.6–1.8% on the fruits) compared to the conventional chemical scab control (0.9% leaves and 0.6% fruits) and was significantly lower than in the untreated control (9.3% on leaves and 11.9 on fruits).