This page has only limited features, please log in for full access.
Plants produce several chemically diverse bioactive substances that may influence the growth and development of other organisms when released into the environment in a phenomenon called allelopathy. Several of these allelopathic species also have reported medicinal properties. In this study, the potential allelopathic effects of more than a hundred medicinal plants from Cambodia were tested using the dish pack method. The dish pack bioassay method specifically targets volatile allelochemicals. Twenty-five species were found to have significant inhibitory effects on lettuce radicle growth. Eleven different plant families, including Iridaceae (2), Apocynaceae (2), Poaceae (2), Sapindaceae, Araceae, Combretaceae, Orchidaceae, Clusiaceae, Zingiberaceae, Rutaceae and Asparagaceae had the plant species with high inhibitory effects. Allophyllus serrulatus had the highest growth inhibitory effect on lettuce radicles more than 60%, followed by Alocasia macrorrhiza, Iris pallida, Terminalia triptera, Wrightia tomentosa, Cymbidium aloifolium, Garcinia villersiana and Kaempferia parviflora. The candidate species were subjected to further studies to identify the volatile allelochemicals in the volatile constituents.
Yourk Sothearith; Kwame Sarpong Appiah; Hossein Mardani; Takashi Motobayashi; Suzuki Yoko; Khou Eang Hourt; Akifumi Sugiyama; Yoshiharu Fujii. Determination of the Allelopathic Potential of Cambodia’s Medicinal Plants Using the Dish Pack Method. Sustainability 2021, 13, 9062 .
AMA StyleYourk Sothearith, Kwame Sarpong Appiah, Hossein Mardani, Takashi Motobayashi, Suzuki Yoko, Khou Eang Hourt, Akifumi Sugiyama, Yoshiharu Fujii. Determination of the Allelopathic Potential of Cambodia’s Medicinal Plants Using the Dish Pack Method. Sustainability. 2021; 13 (16):9062.
Chicago/Turabian StyleYourk Sothearith; Kwame Sarpong Appiah; Hossein Mardani; Takashi Motobayashi; Suzuki Yoko; Khou Eang Hourt; Akifumi Sugiyama; Yoshiharu Fujii. 2021. "Determination of the Allelopathic Potential of Cambodia’s Medicinal Plants Using the Dish Pack Method." Sustainability 13, no. 16: 9062.
SUMMARY Plants produce a large variety of lipophilic metabolites, many of which are secreted by cells and accumulated in apoplasts. The mechanism of secretion remains largely unknown, because hydrophobic metabolites, which may form oil droplets or crystals in cytosol, inducing cell death, cannot be directly secreted by transporters. Moreover, some secondary metabolic lipids react with cytosolic components leading to their decomposition. Lipophilic metabolites should thus be solubilized by matrix lipids and compartmentalized by membrane lipids. The mechanism of lipophilic metabolite secretion was assessed using shikonin, a red naphthoquinone lipid, in Lithospermum erythrorhizon. Cell secretion of shikonin also involved the secretion of about 30% of triacylglycerol (TAG), composed predominantly of saturated fatty acids. Shikonin production was associated with the induction of large amounts of the membrane lipid phosphatidylcholine. Together with in vitro reconstitution, these findings suggest a novel role for TAG as a matrix lipid for the secretion of lipophilic metabolites.
Kanade Tatsumi; Takukji Ichino; Natsumi Isaka; Akifumi Sugiyama; Yozo Okazaki; Yasuhiro Higashi; Masataka Kajikawa; Hideya Fukuzawa; Kiminori Toyooka; Mayuko Sato; Ikuyo Ichi; Koichiro Shimomura; Hiroyuki Ohta; Kazuki Saito; Kazufumi Yazaki. Excretion of triacylglycerol as a matrix lipid facilitating apoplastic accumulation of a lipophilic metabolite shikonin. 2021, 1 .
AMA StyleKanade Tatsumi, Takukji Ichino, Natsumi Isaka, Akifumi Sugiyama, Yozo Okazaki, Yasuhiro Higashi, Masataka Kajikawa, Hideya Fukuzawa, Kiminori Toyooka, Mayuko Sato, Ikuyo Ichi, Koichiro Shimomura, Hiroyuki Ohta, Kazuki Saito, Kazufumi Yazaki. Excretion of triacylglycerol as a matrix lipid facilitating apoplastic accumulation of a lipophilic metabolite shikonin. . 2021; ():1.
Chicago/Turabian StyleKanade Tatsumi; Takukji Ichino; Natsumi Isaka; Akifumi Sugiyama; Yozo Okazaki; Yasuhiro Higashi; Masataka Kajikawa; Hideya Fukuzawa; Kiminori Toyooka; Mayuko Sato; Ikuyo Ichi; Koichiro Shimomura; Hiroyuki Ohta; Kazuki Saito; Kazufumi Yazaki. 2021. "Excretion of triacylglycerol as a matrix lipid facilitating apoplastic accumulation of a lipophilic metabolite shikonin." , no. : 1.
Raspberry ketone is one of the characteristic flavors of raspberry fruits, and it is an important and expensive ingredient in the flavor and fragrance industries. It is present at low levels in plant tissues, and its occurrence is limited to a few taxa. In this context, the stable production of nature-identical raspberry ketone using heterologous synthesis in plants hosts has recently garnered the attention of plant biochemists. In this study, we demonstrate the rational switching of the metabolic flow from anthocyanin pigments to volatile phenylbutanoid production via the phenylpropanoid pathway. This shift led to the efficient and stable production of raspberry ketone and its glycosides via heterologous expression of the biosynthetic enzymes benzalacetone synthase (BAS) and raspberry ketone/zingerone synthase 1 (RZS1) in the transgenic tobacco (Nicotiana tabacum ‘Petit Havana SR-1’). Additionally, we achieved improved product titers by activating the phenylpropanoid pathway with the transcriptional factor, production of anthocyanin pigment 1 (PAP1), from Arabidopsis thaliana. We further demonstrated another metabolic-flow switching by RNA interference (RNAi)-mediated silencing of chalcone synthase (CHS) to increase pathway-intermediate p-coumaroyl-CoA in transgenic tobacco for raspberry-ketone production. The redirection of metabolic flux resulted in transgenic lines producing 0.45 μg/g of raspberry ketone and 4.5 μg/g, on the fresh weight basis, of its glycosides in the flowers. These results suggest that the intracellular enforcement of endogenous substrate supply is an important factor while engineering the phenylpropanoid pathway. This strategy might be useful for the production of other phenylpropanoids/polyketides that are produced via the pathway-intermediate p-coumaroyl-CoA, in tobacco plants.
Takao Koeduka; Sachiho Takarada; Koya Fujii; Akifumi Sugiyama; Kazufumi Yazaki; Masahiro Nishihara; Kenji Matsui. Production of raspberry ketone by redirecting the metabolic flux to the phenylpropanoid pathway in tobacco plants. Metabolic Engineering Communications 2021, 13, e00180 .
AMA StyleTakao Koeduka, Sachiho Takarada, Koya Fujii, Akifumi Sugiyama, Kazufumi Yazaki, Masahiro Nishihara, Kenji Matsui. Production of raspberry ketone by redirecting the metabolic flux to the phenylpropanoid pathway in tobacco plants. Metabolic Engineering Communications. 2021; 13 ():e00180.
Chicago/Turabian StyleTakao Koeduka; Sachiho Takarada; Koya Fujii; Akifumi Sugiyama; Kazufumi Yazaki; Masahiro Nishihara; Kenji Matsui. 2021. "Production of raspberry ketone by redirecting the metabolic flux to the phenylpropanoid pathway in tobacco plants." Metabolic Engineering Communications 13, no. : e00180.
Reductive soil disinfestation (RSD) and soil solarization (SS) were evaluated based on environmental factors, microbiome, and suppression of Fusarium oxysporum in a tomato field soil. Soil environmental factors (moisture content, electric conductivity, pH, and redox potential (RP)) were measured during soil disinfestations. All factors were more strongly influenced by RSD than SS. 16S rRNA amplicon sequencing of RSD- and SS-treated soils was performed. The bacterial communities were taxonomically and functionally distinct depending on treatment methods and periods and significantly correlated with pH and RP. Fifty-four pathways predicted by PICRUSt2 (third level in MetaCyc hierarchy) were significantly different between RSD and SS. Quantitative polymerase chain reaction demonstrated that both treatments equally suppressed F. oxysporum. The growth and yield of tomato cultivated after treatments were similar between RSD and SS. RSD and SS shaped different soil bacterial communities, although the effects on pathogen suppression and tomato plant growth were comparable between treatments. The existence of pathogen-suppressive microbes, other than Clostridia previously reported to have an effect, was suggested. Comparison between RSD and SS provides new aspects of unknown disinfestation patterns and the usefulness of SS as an alternative to RSD.
Masaru Nakayasu; Kyoko Ikeda; Shinichi Yamazaki; Yuichi Aoki; Kazufumi Yazaki; Haruhiko Washida; Akifumi Sugiyama. Two Distinct Soil Disinfestations Differently Modify the Bacterial Communities in a Tomato Field. Agronomy 2021, 11, 1375 .
AMA StyleMasaru Nakayasu, Kyoko Ikeda, Shinichi Yamazaki, Yuichi Aoki, Kazufumi Yazaki, Haruhiko Washida, Akifumi Sugiyama. Two Distinct Soil Disinfestations Differently Modify the Bacterial Communities in a Tomato Field. Agronomy. 2021; 11 (7):1375.
Chicago/Turabian StyleMasaru Nakayasu; Kyoko Ikeda; Shinichi Yamazaki; Yuichi Aoki; Kazufumi Yazaki; Haruhiko Washida; Akifumi Sugiyama. 2021. "Two Distinct Soil Disinfestations Differently Modify the Bacterial Communities in a Tomato Field." Agronomy 11, no. 7: 1375.
Vegetative and reproductive characteristics, fruit yield, and biochemical compounds of six bitter melon cultivars (Iranshahr, Mestisa, No. 486, Local Japanese, Isfahan, and Ilocano) were evaluated under Karaj conditions in Iran. The phytochemical properties of the cultivars were evaluated using both shade-dried and freeze-dried samples at three fruit developmental stages (unripe, semi-ripe, and ripe). There were significant differences in the vegetative and reproductive characteristics among cultivars, where cv. No. 486 was superior to most vegetative attributes. The fruit yield of cultivars varied from 2.98–5.22 kg/plant. The number of days to male and female flower appearance ranged from 19.00–25.33 and from 25–33 days, respectively. The leaf charantin content was in the range of 4.83–11.08 μg/g. Fruit charantin content varied with developmental stage, drying method, and cultivar. The highest charantin content (13.84 ± 3.55 µg/g) was observed at the semi-ripe fruit stage, and it was much higher in the freeze-dried samples than the shade-dried samples. Cultivar No. 486 had the highest (15.43 ± 2.4 µg/g) charantin content, whereas the lowest charantin content (8.51 ± 1.15 µg/g) was recorded in cultivar cv. Local Japanese. The highest total phenol content (25.17 ± 2.27 mg GAE/g) was recorded in freeze-dried samples of ripe fruits of cv. No. 486, whereas the lowest phenol content was detected in the shade-dried samples of semi-ripe fruits of Isfahan. cv. Flavonoid content was higher with the shade-drying method, irrespective of cultivar. In conclusion, considering the fruit yield and active biological compounds in the studied cultivars, cv. No. 486 should be grown commercially because of its higher yield and production of other secondary metabolites.
Akram Valyaie; Majid Azizi; Abdolkarim Kashi; Ramaraj Sathasivam; Sang Park; Akifumi Sugiyama; Takashi Motobayashi; Yoshiharu Fujii. Evaluation of Growth, Yield, and Biochemical Attributes of Bitter Gourd (Momordica charantia L.) Cultivars under Karaj Conditions in Iran. Plants 2021, 10, 1370 .
AMA StyleAkram Valyaie, Majid Azizi, Abdolkarim Kashi, Ramaraj Sathasivam, Sang Park, Akifumi Sugiyama, Takashi Motobayashi, Yoshiharu Fujii. Evaluation of Growth, Yield, and Biochemical Attributes of Bitter Gourd (Momordica charantia L.) Cultivars under Karaj Conditions in Iran. Plants. 2021; 10 (7):1370.
Chicago/Turabian StyleAkram Valyaie; Majid Azizi; Abdolkarim Kashi; Ramaraj Sathasivam; Sang Park; Akifumi Sugiyama; Takashi Motobayashi; Yoshiharu Fujii. 2021. "Evaluation of Growth, Yield, and Biochemical Attributes of Bitter Gourd (Momordica charantia L.) Cultivars under Karaj Conditions in Iran." Plants 10, no. 7: 1370.
Host secondary metabolites have a crucial effect on the taxonomic composition of its associated microbiota. It is estimated that a single plant species produces hundreds of secondary metabolites; however, whether different classes of metabolites have distinctive or common roles in the microbiota assembly remains unclear.
Tomohisa Shimasaki; Sachiko Masuda; Ruben Garrido-Oter; Takashi Kawasaki; Yuichi Aoki; Arisa Shibata; Wataru Suda; Ken Shirasu; Kazufumi Yazaki; Ryohei Thomas Nakano; Akifumi Sugiyama. Tobacco Root Endophytic Arthrobacter Harbors Genomic Features Enabling the Catabolism of Host-Specific Plant Specialized Metabolites. mBio 2021, 12, 1 .
AMA StyleTomohisa Shimasaki, Sachiko Masuda, Ruben Garrido-Oter, Takashi Kawasaki, Yuichi Aoki, Arisa Shibata, Wataru Suda, Ken Shirasu, Kazufumi Yazaki, Ryohei Thomas Nakano, Akifumi Sugiyama. Tobacco Root Endophytic Arthrobacter Harbors Genomic Features Enabling the Catabolism of Host-Specific Plant Specialized Metabolites. mBio. 2021; 12 (3):1.
Chicago/Turabian StyleTomohisa Shimasaki; Sachiko Masuda; Ruben Garrido-Oter; Takashi Kawasaki; Yuichi Aoki; Arisa Shibata; Wataru Suda; Ken Shirasu; Kazufumi Yazaki; Ryohei Thomas Nakano; Akifumi Sugiyama. 2021. "Tobacco Root Endophytic Arthrobacter Harbors Genomic Features Enabling the Catabolism of Host-Specific Plant Specialized Metabolites." mBio 12, no. 3: 1.
Plants are in constant interaction with a myriad of soil microorganisms in the rhizosphere, an area of soil in close contact with plant roots. Recent research has highlighted the importance of plant-specialized metabolites (PSMs) in shaping and modulating the rhizosphere microbiota; however, the molecular mechanisms underlying the establishment and function of the microbiota mostly remain unaddressed. Flavonoids and saponins are a group of PSMs whose biosynthetic pathways have largely been revealed. Although these PSMs are abundantly secreted into the rhizosphere and exert various functions, the secretion mechanisms have not been clarified. This review summarizes the roles of flavonoids and saponins in the rhizosphere with a special focus on interactions between plants and the rhizosphere microbiota. Furthermore, this review introduces recent advancements in the dynamics of these metabolites in the rhizosphere and indicates potential applications of PSMs for crop production and discusses perspectives in this emerging research field.
Akifumi Sugiyama. Flavonoids and saponins in plant rhizospheres: roles, dynamics, and the potential for agriculture. Bioscience, Biotechnology, and Biochemistry 2021, 85, 1919 -1931.
AMA StyleAkifumi Sugiyama. Flavonoids and saponins in plant rhizospheres: roles, dynamics, and the potential for agriculture. Bioscience, Biotechnology, and Biochemistry. 2021; 85 (9):1919-1931.
Chicago/Turabian StyleAkifumi Sugiyama. 2021. "Flavonoids and saponins in plant rhizospheres: roles, dynamics, and the potential for agriculture." Bioscience, Biotechnology, and Biochemistry 85, no. 9: 1919-1931.
Plants produce ∼300 aromatic compounds enzymatically linked to prenyl side chains via C–O bonds. These O-prenylated aromatic compounds have been found in taxonomically distant plant taxa, with some of them being beneficial or detrimental to human health. Although their O-prenyl moieties often play crucial roles in the biological activities of these compounds, no plant gene encoding an aromatic O-prenyltransferase (O-PT) has been isolated to date. This study describes the isolation of an aromatic O-PT gene, CpPT1, belonging to the UbiA superfamily, from grapefruit (Citrus × paradisi, Rutaceae). This gene was shown responsible for the biosynthesis of O-prenylated coumarin derivatives that alter drug pharmacokinetics in the human body. Another coumarin O-PT gene encoding a protein of the same family was identified in Angelica keiskei, an apiaceous medicinal plant containing pharmaceutically active O-prenylated coumarins. Phylogenetic analysis of these O-PTs suggested that aromatic O-prenylation activity evolved independently from the same ancestral gene in these distant plant taxa. These findings shed light on understanding the evolution of plant secondary (specialized) metabolites via the UbiA superfamily.
Ryosuke Munakata; Alexandre Olry; Tomoya Takemura; Kanade Tatsumi; Takuji Ichino; Cloé Villard; Joji Kageyama; Tetsuya Kurata; Masaru Nakayasu; Florence Jacob; Takao Koeduka; Hirobumi Yamamoto; Eiko Moriyoshi; Tetsuya Matsukawa; Jérémy Grosjean; Célia Krieger; Akifumi Sugiyama; Masaharu Mizutani; Frédéric Bourgaud; Alain Hehn; Kazufumi Yazaki. Parallel evolution of UbiA superfamily proteins into aromatic O-prenyltransferases in plants. Proceedings of the National Academy of Sciences 2021, 118, 1 .
AMA StyleRyosuke Munakata, Alexandre Olry, Tomoya Takemura, Kanade Tatsumi, Takuji Ichino, Cloé Villard, Joji Kageyama, Tetsuya Kurata, Masaru Nakayasu, Florence Jacob, Takao Koeduka, Hirobumi Yamamoto, Eiko Moriyoshi, Tetsuya Matsukawa, Jérémy Grosjean, Célia Krieger, Akifumi Sugiyama, Masaharu Mizutani, Frédéric Bourgaud, Alain Hehn, Kazufumi Yazaki. Parallel evolution of UbiA superfamily proteins into aromatic O-prenyltransferases in plants. Proceedings of the National Academy of Sciences. 2021; 118 (17):1.
Chicago/Turabian StyleRyosuke Munakata; Alexandre Olry; Tomoya Takemura; Kanade Tatsumi; Takuji Ichino; Cloé Villard; Joji Kageyama; Tetsuya Kurata; Masaru Nakayasu; Florence Jacob; Takao Koeduka; Hirobumi Yamamoto; Eiko Moriyoshi; Tetsuya Matsukawa; Jérémy Grosjean; Célia Krieger; Akifumi Sugiyama; Masaharu Mizutani; Frédéric Bourgaud; Alain Hehn; Kazufumi Yazaki. 2021. "Parallel evolution of UbiA superfamily proteins into aromatic O-prenyltransferases in plants." Proceedings of the National Academy of Sciences 118, no. 17: 1.
Saponins are the group of plant specialized metabolites which are widely distributed in angiosperm plants and have various biological activities. The present study focused on α-tomatine, a major saponin present in tissues of tomato (Solanum lycopersicum) plants. α-Tomatine is responsible for defense against plant pathogens and herbivores, but its biological function in the rhizosphere remains unknown. Secretion of tomatine was higher at the early growth than the green-fruit stage in hydroponically grown plants, and the concentration of tomatine in the rhizosphere of field-grown plants was higher than that of the bulk soil at all growth stages. The effects of tomatine and its aglycone tomatidine on the bacterial communities in the soil were evaluated in vitro, revealing that both compounds influenced the microbiome in a concentration-dependent manner. Numerous bacterial families were influenced in tomatine/tomatidine-treated soil as well as in the tomato rhizosphere. Sphingomonadaceae species, which are commonly observed and enriched in tomato rhizospheres in the fields, were also enriched in tomatine- and tomatidine-treated soils. Moreover, a jasmonate-responsive ETHYLENE RESPONSE FACTOR 4 mutant associated with low tomatine production caused the root-associated bacterial communities to change with a reduced abundance of Sphingomonadaceae. Taken together, our results highlight the role of tomatine in shaping the bacterial communities of the rhizosphere and suggest additional functions of tomatine in belowground biological communication.
Masaru Nakayasu; Kohei Ohno; Kyoko Takamatsu; Yuichi Aoki; Shinichi Yamazaki; Hisabumi Takase; Tsubasa Shoji; Kazufumi Yazaki; Akifumi Sugiyama. Tomato roots secrete tomatine to modulate the bacterial assemblage of the rhizosphere. Plant Physiology 2021, 186, 270 -284.
AMA StyleMasaru Nakayasu, Kohei Ohno, Kyoko Takamatsu, Yuichi Aoki, Shinichi Yamazaki, Hisabumi Takase, Tsubasa Shoji, Kazufumi Yazaki, Akifumi Sugiyama. Tomato roots secrete tomatine to modulate the bacterial assemblage of the rhizosphere. Plant Physiology. 2021; 186 (1):270-284.
Chicago/Turabian StyleMasaru Nakayasu; Kohei Ohno; Kyoko Takamatsu; Yuichi Aoki; Shinichi Yamazaki; Hisabumi Takase; Tsubasa Shoji; Kazufumi Yazaki; Akifumi Sugiyama. 2021. "Tomato roots secrete tomatine to modulate the bacterial assemblage of the rhizosphere." Plant Physiology 186, no. 1: 270-284.
Analyses of metabolite secretions by field-grown plants remain scarce. We analyzed daidzein secretion by field-grown soybean. Daidzein secretion was higher during early vegetative stages than reproductive stages, a trend that was also seen for hydroponically grown soybean. Daidzein secretion was up to 10 000-fold higher under field conditions than hydroponic conditions, leading to a more accurate simulation of rhizosphere daidzein content.
Miwako Toyofuku; Fuki Okutani; Masaru Nakayasu; Shoichiro Hamamoto; Hisabumi Takase; Kazufumi Yazaki; Akifumi Sugiyama. Enhancement of developmentally regulated daidzein secretion from soybean roots in field conditions as compared with hydroponic culture. Bioscience, Biotechnology, and Biochemistry 2021, 85, 1165 -1169.
AMA StyleMiwako Toyofuku, Fuki Okutani, Masaru Nakayasu, Shoichiro Hamamoto, Hisabumi Takase, Kazufumi Yazaki, Akifumi Sugiyama. Enhancement of developmentally regulated daidzein secretion from soybean roots in field conditions as compared with hydroponic culture. Bioscience, Biotechnology, and Biochemistry. 2021; 85 (5):1165-1169.
Chicago/Turabian StyleMiwako Toyofuku; Fuki Okutani; Masaru Nakayasu; Shoichiro Hamamoto; Hisabumi Takase; Kazufumi Yazaki; Akifumi Sugiyama. 2021. "Enhancement of developmentally regulated daidzein secretion from soybean roots in field conditions as compared with hydroponic culture." Bioscience, Biotechnology, and Biochemistry 85, no. 5: 1165-1169.
Phnom Kulen National Park, in north-western Cambodia, has huge richness in biodiversity and medicinal value. One hundred and ninety-five (195) medicinal plant species were collected from the national park to examine allelopathic potentials by using the sandwich method, a specific bioassay for the evaluation of leachates from plants. The study found 58 out of 195 medicinal plant species showed significant inhibitory effects on lettuce radicle elongation as evaluated by standard deviation variance based on the normal distribution. Three species including Iris pallida (4% of control), Parabarium micranthum (7.5% of control), and Peliosanthes teta (8.2% of control) showed strong inhibition of lettuce radicle elongation less than 10% of the control. The results presented could present as a benchmark for isolation and identification of allelochemicals among medicinal plants used in Cambodia.
Yourk Sothearith; Kwame Appiah; Takashi Motobayashi; Izumi Watanabe; Chan Somaly; Akifumi Sugiyama; Yoshiharu Fujii. Evaluation of Allelopathic Potentials from Medicinal Plant Species in Phnom Kulen National Park, Cambodia by the Sandwich Method. Sustainability 2020, 13, 264 .
AMA StyleYourk Sothearith, Kwame Appiah, Takashi Motobayashi, Izumi Watanabe, Chan Somaly, Akifumi Sugiyama, Yoshiharu Fujii. Evaluation of Allelopathic Potentials from Medicinal Plant Species in Phnom Kulen National Park, Cambodia by the Sandwich Method. Sustainability. 2020; 13 (1):264.
Chicago/Turabian StyleYourk Sothearith; Kwame Appiah; Takashi Motobayashi; Izumi Watanabe; Chan Somaly; Akifumi Sugiyama; Yoshiharu Fujii. 2020. "Evaluation of Allelopathic Potentials from Medicinal Plant Species in Phnom Kulen National Park, Cambodia by the Sandwich Method." Sustainability 13, no. 1: 264.
Isoflavones and soyasaponins are major specialized metabolites accumulated in soybean roots and secreted into the rhizosphere. Unlike the biosynthetic pathway, the transporters involved in metabolite secretion remain unknown. The developmental regulation of isoflavone and soyasaponin secretions has been recently reported, but the diurnal regulation of their biosynthesis and secretion still needs to be further studied. To address these challenges, we conducted transcriptome and metabolite analysis using hydroponically grown soybean plants at 6‐hr intervals for 48 hr in a 12‐hr‐light/12‐hr‐dark condition. Isoflavone and soyasaponin biosynthetic genes showed opposite patterns in the root tissues; that is, the former genes are highly expressed in the daytime, while the latter ones are strongly induced at nighttime. GmMYB176 encoding a transcription factor of isoflavone biosynthesis was upregulated from ZT0 (6:00 a.m.) to ZT6 (12:00 a.m.), followed by the induction of isoflavone biosynthetic genes at ZT6. The isoflavone aglycone content in the roots accordingly increased from ZT6 to ZT18 (0:00 a.m.). The isoflavone aglycone content in root exudates was kept consistent throughout the day, whereas that of glucosides increased at ZT6, which reflected the decreased expression of the gene encoding beta‐glucosidase involved in the hydrolysis of apoplast‐localized isoflavone conjugates. Co‐expression analysis revealed that those isoflavone and soyasaponin biosynthetic genes formed separate clusters, which exhibited a correlation to ABC and MATE transporter genes. In summary, the results in this study indicated the diurnal regulation of isoflavone biosynthesis in soybean roots and the putative transporter genes responsible for isoflavone and soyasaponin transport.
Hinako Matsuda; Masaru Nakayasu; Yuichi Aoki; Shinichi Yamazaki; Atsushi J. Nagano; Kazufumi Yazaki; Akifumi Sugiyama. Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots. Plant Direct 2020, 4, 1 .
AMA StyleHinako Matsuda, Masaru Nakayasu, Yuichi Aoki, Shinichi Yamazaki, Atsushi J. Nagano, Kazufumi Yazaki, Akifumi Sugiyama. Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots. Plant Direct. 2020; 4 (11):1.
Chicago/Turabian StyleHinako Matsuda; Masaru Nakayasu; Yuichi Aoki; Shinichi Yamazaki; Atsushi J. Nagano; Kazufumi Yazaki; Akifumi Sugiyama. 2020. "Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots." Plant Direct 4, no. 11: 1.
Soyasaponins are triterpenoid saponins widely found in legume plants. These compounds have drawn considerable attention because they have various activities beneficial for human health, and their biosynthesis has been actively studied. In our previous study, we found that legume plants including soybean secrete soyasaponins from the roots in hydroponic culture throughout the growth period, but the physiological roles of soyasaponins in the rhizosphere and their fate in soil after exudation have remained unknown. This study demonstrates that soyasaponins are secreted from the roots of field‐grown soybean, and soyasaponin Bb is the major soyasaponin detected in the rhizosphere. In vitro analysis of the distribution coefficient suggested that soyasaponin Bb can diffuse over longer distances in the soil in comparison with daidzein, which is a typical isoflavone secreted from soybean roots. The degradation rate of soyasaponin Bb in soil was slightly faster than that of daidzein, whereas no soyasaponin Bb degradation was observed in autoclaved soil, suggesting that microbes utilize soyasaponins in the rhizosphere. Bacterial community composition was clearly influenced by soyasaponin Bb, and potential plant growth–promoting rhizobacteria such as Novosphingobium were significantly enriched in both soyasaponin Bb–treated soil and the soybean rhizosphere. These results strongly suggest that soyasaponin Bb plays an important role in the enrichment of certain microbes in the soybean rhizosphere.
Teruhisa Fujimatsu; Keiji Endo; Kazufumi Yazaki; Akifumi Sugiyama. Secretion dynamics of soyasaponins in soybean roots and effects to modify the bacterial composition. Plant Direct 2020, 4, 1 .
AMA StyleTeruhisa Fujimatsu, Keiji Endo, Kazufumi Yazaki, Akifumi Sugiyama. Secretion dynamics of soyasaponins in soybean roots and effects to modify the bacterial composition. Plant Direct. 2020; 4 (9):1.
Chicago/Turabian StyleTeruhisa Fujimatsu; Keiji Endo; Kazufumi Yazaki; Akifumi Sugiyama. 2020. "Secretion dynamics of soyasaponins in soybean roots and effects to modify the bacterial composition." Plant Direct 4, no. 9: 1.
Plants produce approximately 300 aromatic molecules enzymatically linked to prenyl side chains via C-O bonds. These O-prenylated aromatics have been found in taxonomically distant plant taxa as compounds beneficial or detrimental to human health, with O-prenyl moieties often playing crucial roles in their biological activities. To date, however, no plant gene encoding an aromatic O-prenyltransferase (O-PT) has been described. This study describes the isolation of an aromatic O-PT gene, CpPT1, belonging to the UbiA superfamily, from grapefruit (Citrus paradisi, Rutaceae). This gene is responsible for the biosynthesis of O-prenylated coumarin derivatives that alter drug pharmacokinetics in the human body. Another coumarin O-PT gene of the same protein family was identified in Angelica keiskei, an apiaceous medicinal plant containing pharmaceutically active O-prenylated coumarins. Phylogenetic analysis of these O-PTs suggested that aromatic O-prenylation activity evolved independently from the same ancestral gene in these distant plant taxa. These findings shed light on understanding the evolution of plant secondary metabolites via the UbiA superfamily.
Ryosuke Munakata; Alexandre Olry; Tomoya Takemura; Kanade Tatsumi; Takuji Ichino; Cloé Villard; Joji Kageyama; Tetsuya Kurata; Masaru Nakayasu; Florence Jacob; Takao Koeduka; Hirobumi Yamamoto; Eiko Moriyoshi; Tetsuya Matsukawa; Jeremy Grosjean; Célia Krieger; Akifumi Sugiyama; Masaharu Mizutani; Frédéric Bourgaud; Alain Hehn; Kazufumi Yazaki. Parallel evolution of UbiA superfamily proteins into aromatic O-prenyltransferases in plants. 2020, 1 .
AMA StyleRyosuke Munakata, Alexandre Olry, Tomoya Takemura, Kanade Tatsumi, Takuji Ichino, Cloé Villard, Joji Kageyama, Tetsuya Kurata, Masaru Nakayasu, Florence Jacob, Takao Koeduka, Hirobumi Yamamoto, Eiko Moriyoshi, Tetsuya Matsukawa, Jeremy Grosjean, Célia Krieger, Akifumi Sugiyama, Masaharu Mizutani, Frédéric Bourgaud, Alain Hehn, Kazufumi Yazaki. Parallel evolution of UbiA superfamily proteins into aromatic O-prenyltransferases in plants. . 2020; ():1.
Chicago/Turabian StyleRyosuke Munakata; Alexandre Olry; Tomoya Takemura; Kanade Tatsumi; Takuji Ichino; Cloé Villard; Joji Kageyama; Tetsuya Kurata; Masaru Nakayasu; Florence Jacob; Takao Koeduka; Hirobumi Yamamoto; Eiko Moriyoshi; Tetsuya Matsukawa; Jeremy Grosjean; Célia Krieger; Akifumi Sugiyama; Masaharu Mizutani; Frédéric Bourgaud; Alain Hehn; Kazufumi Yazaki. 2020. "Parallel evolution of UbiA superfamily proteins into aromatic O-prenyltransferases in plants." , no. : 1.
Isoflavones and soyasaponins are major specialized metabolites accumulated in soybean roots and secreted into the rhizosphere. Unlike the biosynthetic pathway, the transporters involved in metabolite secretion remain unknown. The developmental regulation of isoflavone and soyasaponin secretions has been recently reported, but the diurnal regulation of their biosynthesis and secretion still needs to be further studied. To address these challenges, we conducted transcriptome and metabolite analysis using hydroponically grown soybean plants at 6-hour intervals for 48 hours in a 12-h-light/12-h-dark condition. Isoflavone and soyasaponin biosynthetic genes showed opposite patterns in the root tissues; that is, the former genes are highly expressed in daytime, while the latter ones are strongly induced at nighttime. GmMYB176 encoding a transcription factor of isoflavone biosynthesis was upregulated from ZT0 (6:00 am) to ZT6 (12:00 am), followed by the induction of isoflavone biosynthetic genes at ZT6. The isoflavone aglycone content in the roots accordingly increased from ZT6 to ZT18 (0:00 am), accompanied by an increase in glucoside levels that peaked at ZT0. The isoflavone aglycone content in root exudates was kept consistent throughout the day, whereas that of glucosides increased at ZT6, which reflected the decreased expression of the gene encoding beta-glucosidase involved in the hydrolysis of apoplast-localized isoflavone conjugates. Co-expression analysis revealed that those isoflavone and soyasaponin biosynthetic genes formed separate clusters, which exhibited a correlation to ABC and MATE transporter genes. As summary, the results in this study indicated the diurnal regulation of isoflavone biosynthesis in soybean roots and the putative transporter genes responsible for isoflavone and soyasaponin transport.
Hinako Matsuda; Masaru Nakayasu; Yuichi Aoki; Shinichi Yamazaki; Atsushi J. Nagano; Kazufumi Yazaki; Akifumi Sugiyama. Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots. 2020, 1 .
AMA StyleHinako Matsuda, Masaru Nakayasu, Yuichi Aoki, Shinichi Yamazaki, Atsushi J. Nagano, Kazufumi Yazaki, Akifumi Sugiyama. Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots. . 2020; ():1.
Chicago/Turabian StyleHinako Matsuda; Masaru Nakayasu; Yuichi Aoki; Shinichi Yamazaki; Atsushi J. Nagano; Kazufumi Yazaki; Akifumi Sugiyama. 2020. "Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots." , no. : 1.
Inter-organismal communications below ground, such as plant–microbe interactions in the rhizosphere, affect plant growth. Metabolites are shown to play important roles in biological communication, but there still remain a large number of metabolites in soil to be uncovered. Metabolomics, a technique for the comprehensive analysis of metabolites in samples, may uncover the molecules that intermediate these interactions. We conducted a multivariate analysis using liquid chromatography (LC)—mass spectrometry (MS)-based untargeted metabolomics in several soil samples and also targeted metabolome analysis for the identification of the candidate compounds in soil. We identified okaramine A, B, and C in the rhizosphere soil of hairy vetch. Okaramines are indole alkaloids first identified in soybean pulp (okara) inoculated with Penicillium simplicissimum AK-40 and are insecticidal. Okaramine B was detected in the rhizosphere from an open field growing hairy vetch. Okaramine B was also detected in both bulk and rhizosphere soils of soybean grown following hairy vetch, but not detected in soils of soybean without hairy vetch growth. These results suggested that okaramines might be involved in indirect defense of plants against insects. To our knowledge, this is the first report of okaramines in the natural environment. Untargeted and targeted metabolomics would be useful to uncover the chemistry of the rhizosphere.
Nozomu Sakurai; Hossein Mardani-Korrani; Masaru Nakayasu; Kazuhiko Matsuda; Kumiko Ochiai; Masaru Kobayashi; Yusuke Tahara; Takeshi Onodera; Yuichi Aoki; Takashi Motobayashi; Masakazu Komatsuzaki; Makoto Ihara; Daisuke Shibata; Yoshiharu Fujii; Akifumi Sugiyama. Metabolome Analysis Identified Okaramines in the Soybean Rhizosphere as a Legacy of Hairy Vetch. Frontiers in Genetics 2020, 11, 114 .
AMA StyleNozomu Sakurai, Hossein Mardani-Korrani, Masaru Nakayasu, Kazuhiko Matsuda, Kumiko Ochiai, Masaru Kobayashi, Yusuke Tahara, Takeshi Onodera, Yuichi Aoki, Takashi Motobayashi, Masakazu Komatsuzaki, Makoto Ihara, Daisuke Shibata, Yoshiharu Fujii, Akifumi Sugiyama. Metabolome Analysis Identified Okaramines in the Soybean Rhizosphere as a Legacy of Hairy Vetch. Frontiers in Genetics. 2020; 11 ():114.
Chicago/Turabian StyleNozomu Sakurai; Hossein Mardani-Korrani; Masaru Nakayasu; Kazuhiko Matsuda; Kumiko Ochiai; Masaru Kobayashi; Yusuke Tahara; Takeshi Onodera; Yuichi Aoki; Takashi Motobayashi; Masakazu Komatsuzaki; Makoto Ihara; Daisuke Shibata; Yoshiharu Fujii; Akifumi Sugiyama. 2020. "Metabolome Analysis Identified Okaramines in the Soybean Rhizosphere as a Legacy of Hairy Vetch." Frontiers in Genetics 11, no. : 114.
Plant roots nurture a wide variety of microbes via exudation of metabolites, shaping the rhizosphere#s microbial community. Despite the importance of plant specialized metabolites in the assemblage and function of microbial communities in the rhizosphere, little is known of how far the effects of these metabolites extend through the soil. We employed a fluid model to simulate the spatiotemporal distribution of daidzein, an isoflavone secreted from soybean roots, and validated using soybeans grown in a rhizobox. We then analyzed how daidzein affects bacterial communities using soils artificially treated with daidzein. Simulation of daidzein distribution showed that it was only present within a few millimeters of root surfaces. After 14 days in a rhizobox, daidzein was only present within 2 mm of root surfaces. Soils with different concentrations of daidzein showed different community composition, with reduced α‐diversity in daidzein‐treated soils. Bacterial communities of daidzein‐treated soils were closer to those of the soybean rhizosphere than those of bulk soils. This study highlighted the limited distribution of daidzein within a few millimeters of root surfaces, and demonstrated a novel role of daidzein in assembling bacterial communities in the rhizosphere by acting as more of a repellant than an attractant.
Fuki Okutani; Shoichiro Hamamoto; Yuichi Aoki; Masaru Nakayasu; Naoto Nihei; Taku Nishimura; Kazufumi Yazaki; Akifumi Sugiyama. Rhizosphere modelling reveals spatiotemporal distribution of daidzein shaping soybean rhizosphere bacterial community. Plant, Cell & Environment 2019, 43, 1036 -1046.
AMA StyleFuki Okutani, Shoichiro Hamamoto, Yuichi Aoki, Masaru Nakayasu, Naoto Nihei, Taku Nishimura, Kazufumi Yazaki, Akifumi Sugiyama. Rhizosphere modelling reveals spatiotemporal distribution of daidzein shaping soybean rhizosphere bacterial community. Plant, Cell & Environment. 2019; 43 (4):1036-1046.
Chicago/Turabian StyleFuki Okutani; Shoichiro Hamamoto; Yuichi Aoki; Masaru Nakayasu; Naoto Nihei; Taku Nishimura; Kazufumi Yazaki; Akifumi Sugiyama. 2019. "Rhizosphere modelling reveals spatiotemporal distribution of daidzein shaping soybean rhizosphere bacterial community." Plant, Cell & Environment 43, no. 4: 1036-1046.
Furanocoumarins (FCs) are plant‐specialized metabolites with potent allelochemical properties. The distribution of FCs is scattered with a chemotaxonomical tendency towards four distant families with highly similar FC pathways. The mechanism by which this pathway emerged and spread in plants has not been elucidated.Furanocoumarin biosynthesis was investigated in Ficus carica (fig, Moraceae), focusing on the first committed reaction catalysed by an umbelliferone dimethylallyltransferase (UDT). Comparative RNA‐seq analysis among latexes of different fig organs led to the identification of a UDT. The phylogenetic relationship of this UDT to previously reported Apiaceae UDTs was evaluated.The expression pattern of F. carica prenyltransferase 1 (FcPT1) was related to the FC contents in different latexes. Enzymatic characterization demonstrated that one of the main functions of FcPT1 is UDT activity. Phylogenetic analysis suggested that FcPT1 and Apiaceae UDTs are derived from distinct ancestors, although they both belong to the UbiA superfamily. These findings are supported by significant differences in the related gene structures.This report describes the identification of FcPT1 involved in FC biosynthesis in fig and provides new insights into multiple origins of the FC pathway and, more broadly, into the adaptation of plants to their environments.
Ryosuke Munakata; Sakihito Kitajima; Andréïna Nuttens; Kanade Tatsumi; Tomoya Takemura; Takuji Ichino; Gianni Galati; Sonia Vautrin; Hélène Bergès; Jérémy Grosjean; Frédéric Bourgaud; Akifumi Sugiyama; Alain Hehn; Kazufumi Yazaki. Convergent evolution of the UbiA prenyltransferase family underlies the independent acquisition of furanocoumarins in plants. New Phytologist 2019, 225, 2166 -2182.
AMA StyleRyosuke Munakata, Sakihito Kitajima, Andréïna Nuttens, Kanade Tatsumi, Tomoya Takemura, Takuji Ichino, Gianni Galati, Sonia Vautrin, Hélène Bergès, Jérémy Grosjean, Frédéric Bourgaud, Akifumi Sugiyama, Alain Hehn, Kazufumi Yazaki. Convergent evolution of the UbiA prenyltransferase family underlies the independent acquisition of furanocoumarins in plants. New Phytologist. 2019; 225 (5):2166-2182.
Chicago/Turabian StyleRyosuke Munakata; Sakihito Kitajima; Andréïna Nuttens; Kanade Tatsumi; Tomoya Takemura; Takuji Ichino; Gianni Galati; Sonia Vautrin; Hélène Bergès; Jérémy Grosjean; Frédéric Bourgaud; Akifumi Sugiyama; Alain Hehn; Kazufumi Yazaki. 2019. "Convergent evolution of the UbiA prenyltransferase family underlies the independent acquisition of furanocoumarins in plants." New Phytologist 225, no. 5: 2166-2182.
The rhizosphere, which is the region of soil adjacent to plant roots, is affected by the activities of both plant roots and associated microorganisms which cause changes in soil properties including nutrient mineral composition. Accordingly, the actual availability of plant nutrients may not be the same as that estimated on the basis of bulk soil analysis. However, the extent and manner in which the availability of plant nutrients in bulk and rhizosphere soils differ remain unclear. Therefore, the present study defined the rhizosphere as the soil adhered to plant roots, established a set of small-scale protocols for analyzing the nutrient minerals of small soil samples, and then characterized the rhizosphere soil of sorghum, Sorghum bicolor (L.) Moench. The mineral contents of the bulk and rhizosphere soil differed significantly, with nutrient contents generally greater in the rhizosphere, and particularly remarkable accumulation was observed in regards to ammonium ion and exchangeable potassium concentrations. Such accumulation might be due, in part, to the greater per weight surface areas of rhizosphere soil particles, but other mechanisms, including the accumulation of organic matter, could also be involved.
Shinichi Yamazaki; Kumiko Ochiai; Junko Motokawa; Shoichiro Hamamoto; Akifumi Sugiyama; Masaru Kobayashi. Properties of rhizosphere soil associated with herbaceous plant roots analyzed using small-scale protocols. 2019, 800664 .
AMA StyleShinichi Yamazaki, Kumiko Ochiai, Junko Motokawa, Shoichiro Hamamoto, Akifumi Sugiyama, Masaru Kobayashi. Properties of rhizosphere soil associated with herbaceous plant roots analyzed using small-scale protocols. . 2019; ():800664.
Chicago/Turabian StyleShinichi Yamazaki; Kumiko Ochiai; Junko Motokawa; Shoichiro Hamamoto; Akifumi Sugiyama; Masaru Kobayashi. 2019. "Properties of rhizosphere soil associated with herbaceous plant roots analyzed using small-scale protocols." , no. : 800664.
Purine permeases (PUPs) mediate the proton-coupled uptake of nucleotide bases and their derivatives into cytosol. PUPs facilitate uptake of adenine, cytokinins and nicotine. Caffeine, a purine alkaloid derived from xanthosine, occurs in only a few eudicot species, including coffee, cacao, and tea. Although caffeine is not an endogenous metabolite in Arabidopsis and rice, AtPUP1 and OsPUP7 were suggested to transport caffeine. In this study, we identified 15 PUPs in the genome of Coffea canephora. Direct uptake measurements in yeast demonstrated that CcPUP1 and CcPUP5 facilitate adenine – but not caffeine – transport. Adenine uptake was pH-dependent, with increased activity at pH 3 and 4, and inhibited by nigericin, a potassium–proton ionophore, suggesting that CcPUP1 and CcPUP5 function as proton-symporters. Furthermore, adenine uptake was not competitively inhibited by an excess amount of caffeine, which implies that PUPs of C. canephora have evolved to become caffeine-insensitive to promote efficient uptake of adenine into cytosol.
Hirofumi Kakegawa; Nobukazu Shitan; Hiroaki Kusano; Shinjiro Ogita; Kazufumi Yazaki; Akifumi Sugiyama. Uptake of adenine by purine permeases of Coffea canephora. Bioscience, Biotechnology, and Biochemistry 2019, 83, 1300 -1305.
AMA StyleHirofumi Kakegawa, Nobukazu Shitan, Hiroaki Kusano, Shinjiro Ogita, Kazufumi Yazaki, Akifumi Sugiyama. Uptake of adenine by purine permeases of Coffea canephora. Bioscience, Biotechnology, and Biochemistry. 2019; 83 (7):1300-1305.
Chicago/Turabian StyleHirofumi Kakegawa; Nobukazu Shitan; Hiroaki Kusano; Shinjiro Ogita; Kazufumi Yazaki; Akifumi Sugiyama. 2019. "Uptake of adenine by purine permeases of Coffea canephora." Bioscience, Biotechnology, and Biochemistry 83, no. 7: 1300-1305.